FastQCFastQC Report
Thu 26 May 2016
SRR2099204_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099204_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757016
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAAGCTTTGATGGGTTACGAGCCTACATTCAAGGAACTGAA24480.3233749352721739No Hit
CGGCAGCTTCGCGGCCACGCCGTGTCGGCGCCGAACACCGGCG16870.22284865841673096No Hit
CGTAAGCTACAGCAGCTCAGGTGAACCCAGTAATAATTTTATA13440.17753918014942882No Hit
CGTAAGCTAGTTTAAGTGCTGATGTGTATTACAACGCAAGCTT11290.14913819522969132No Hit
CGTAAGCCTTACTGGATTACAGGCGTGCGCCACCACGCCCGGC11240.14847770720830206No Hit
CGTCAGCCAGGGGAGACGGCTCGCGCGATGATGGGCAAGCACC10240.13526794678051718No Hit
CGTAAGCTGTTATGGAGAAGAAAGTGGAGGCAAAGAAGGAAGA9940.13130501865218172No Hit
CGTAAGCTTATTCGGTTTGTATCTTAGGAGCAATAGGCTCTTC9930.13117292104790387No Hit
CGTAAGCTGGTGCCTGGGTTATGGGCTTTGCCATTGACTCATA9900.13077662823507033No Hit
CGTAAGCTACCCATAATGATGTGCCAATGGAGGTCCATAAATT9230.12192608874845447No Hit
CGTAAGCTAGTTCGGTGCTTAGCACATAGTGAATGGAGATATT8930.117963160620119No Hit
CGTAAGCTTATGCGTGCGCTTTGGCGAAGAGCTCGATCGCGTC8730.11532120853456201No Hit
CGTAAGCTTGTTAGGCGCCTGTAGTCCCAGCTACTTGGGAGGC7900.10435710737950056No Hit
CGTAAGCTAGGTAAGTTTGTGACCAGCCTGGCCAACATGACAA7610.10052627685544295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGG251.229471E-437.019189
GAGCGGA200.001836857837.019189
TTTACGC200.001837454437.016738
TTGTACG200.001837454437.016738
TCTAGCG501.7826096E-1037.016738
TGCGATA403.8151484E-837.016738
TGCGAAG251.2299496E-437.016738
CTAGCCG900.037.016737
AACCGTA2600.037.0167274
TAGCGGG650.037.0167278
AACGTAC251.234264E-436.99471710
CGCTAAG251.234264E-436.99471710
GGCCGAA403.8351573E-836.99471310
AGGGCGC403.8351573E-836.99471313
ATAAGCG2400.036.9727252
TAAGCTA130100.036.9289323
TAAGCTG199650.036.819623
TAAGCTC127350.036.5056463
CGTAAGC646650.036.4181181
GTAAGCT635150.036.390612
Produced by FastQC (version 0.11.5)