SRR2099203_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2099203_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28651
Sequences flagged as poor quality	0
Sequence length	43
%GC	46

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTAAGCTGCGACAATTAGGGCAGGGGCAAGTGTAGGGGCAGC	101	0.3525182367107605	No Hit
CGTAAGCTCGATCGTTCCGACAAGTGCAGCTGCTGTGCCTGAA	74	0.2582806882831315	No Hit
CGTAAGCTCCAACGTTTCAGAAAGGCCTGAATGAGCTCTTGAA	62	0.21639733342640743	No Hit
CGTAAGCTGTGCGAATTCGAAGCTTTCTTCAAAAAGCTCTCAC	54	0.18847509685525812	No Hit
CGGCAGCTTCGCGGCCACGCCGTGTCGGCGCCGAACACCGGCG	52	0.18149453771247076	No Hit
CGTAAGCTCGTCTGTTGCCTAATGGTGGCCTTTCTCATCCTCT	45	0.1570625807127151	No Hit
CTTAAGCTATCGTAGTCCGACGATCTTCGTCGTACTGTAGAAC	44	0.15357230114132142	No Hit
CGTAAGCTTTGTTGATTGGGCTACATCCATCTGTCATCTGAAG	42	0.1465917419985341	No Hit
CGTAAGCTCGTGCGTTTGGCGTGGCTGAGCCAAGAAAGAAGGC	41	0.14310146242714042	No Hit
CGTAAGCTTGCCTAGTCCGACGATCAGTGCCGTAAGCTTTTTT	41	0.14310146242714042	No Hit
CGTAAGCTTTGATGGGTTACGAGCCTACATTCAAGGAACTGAA	40	0.13961118285574675	No Hit
CGTAAGCTATTATGTTTCTAACATAGTTCATTCGCAGGGTTGT	38	0.13263062371295942	No Hit
CGTAAGCTCTGAAATTCCGACGATCACCGTCGTAATGTAGAAC	36	0.12565006457017205	No Hit
CGTAAGCTATGCGGGTCCGACGATCGTCGTCGTACTGTAGAAC	36	0.12565006457017205	No Hit
CGTAAGCAGAAGTAATACGACTCACTATAGTGAGTCGTATTAC	35	0.1221597849987784	No Hit
CGTAAGCTGATCCGTTGCGTTCTTGATCTTGTTTGCCATGTAA	35	0.1221597849987784	No Hit
CGTAAGCTGCATAAATTCGGGAAAAGCTGGCCAAAATGTACAA	33	0.11517922585599107	No Hit
CGTAAGCTTATTCGGTTTGTATCTTAGGAGCAATAGGCTCTTC	31	0.10819866671320372	No Hit
CGTAAGCTAAACTGCTTCGGAGGTCGTGGGTGACAAGTTGGTG	31	0.10819866671320372	No Hit
CGTAAGCTACAGCAGCTCAGGTGAACCCAGTAATAATTTTATA	31	0.10819866671320372	No Hit
CGTAAGCTAGTTTAAGTGCTGATGTGTATTACAACGCAAGCTT	30	0.10470838714181005	No Hit
CGTAAGCTACAACGGTCCGACGATCATTGTCGTACTGTAGAAC	30	0.10470838714181005	No Hit
CGTAAGCTGCTCCGTTTAGATCTAAAAAGTCCCACCTCAGTTT	29	0.10121810757041638	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTTGC	55	1.0913936E-11	37.0	5
AGCTCCT	55	1.0913936E-11	37.0	5
AGCTCCG	40	3.6150595E-8	37.0	5
AGCTTCC	35	5.3802796E-7	37.0	5
AGCTTAG	80	0.0	37.0	5
TAAGCTG	920	0.0	37.0	3
TAAGCTC	550	0.0	37.0	3
TAAGCTA	440	0.0	37.0	3
ATTAGGG	20	0.0018121459	37.0	15
AGCTATC	25	1.2038397E-4	37.0	5
AGCTAGC	35	5.3802796E-7	37.0	5
AGCTAGA	20	0.0018121459	37.0	5
GCTGGTA	20	0.0018121459	37.0	6
AGCTACT	55	1.0913936E-11	37.0	5
AGCTAAG	25	1.2038397E-4	37.0	5
AGCTAAA	20	0.0018121459	37.0	5
GCTGGCG	30	8.032481E-6	37.0	6
TGTCATG	20	0.0018121459	37.0	8
AATTAGG	25	1.2038397E-4	37.0	14
GAAGATT	20	0.0018121459	37.0	11

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content