##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099203_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28651 Sequences flagged as poor quality 0 Sequence length 43 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.23981710935046 37.0 37.0 37.0 35.0 37.0 2 36.120554256395934 37.0 37.0 37.0 35.0 37.0 3 35.60221283724827 37.0 35.0 37.0 35.0 37.0 4 37.19573487836376 39.0 37.0 39.0 34.0 39.0 5 36.99120449548009 39.0 37.0 39.0 34.0 39.0 6 37.19946947750515 39.0 37.0 39.0 34.0 39.0 7 37.79131618442637 39.0 38.0 39.0 35.0 39.0 8 37.646644096192105 39.0 38.0 39.0 35.0 39.0 9 32.1540260374856 33.0 31.0 34.0 31.0 34.0 10 32.33189068444383 34.0 31.0 34.0 31.0 34.0 11 32.35796307284213 34.0 31.0 34.0 31.0 34.0 12 35.865379916931346 37.0 35.0 37.0 35.0 37.0 13 35.878293951345505 37.0 35.0 37.0 35.0 37.0 14 31.026281805172594 31.0 30.0 31.0 30.0 34.0 15 30.616592789082407 31.0 30.0 31.0 27.0 34.0 16 29.600712017032563 31.0 28.0 31.0 25.0 34.0 17 32.579142089281355 35.0 30.0 35.0 26.0 37.0 18 34.01668353635126 35.0 33.0 37.0 30.0 37.0 19 32.529091480227564 35.0 30.0 36.0 25.0 37.0 20 32.03399532302537 35.0 30.0 36.0 25.0 37.0 21 32.255837492583154 35.0 30.0 36.0 25.0 37.0 22 33.54856724023594 35.0 31.0 39.0 25.0 39.0 23 34.139715891242886 37.0 32.0 39.0 27.0 39.0 24 34.67875466824893 37.0 33.0 39.0 27.0 39.0 25 35.18903354158668 37.0 34.0 39.0 30.0 39.0 26 35.341384244878014 37.0 34.0 39.0 30.0 39.0 27 36.66706223168476 38.0 35.0 40.0 31.0 41.0 28 37.06575686712506 38.0 36.0 40.0 32.0 41.0 29 37.37551917908625 38.0 36.0 40.0 32.0 41.0 30 37.5341872884018 39.0 36.0 40.0 33.0 41.0 31 37.79962304980629 39.0 36.0 40.0 33.0 41.0 32 38.16983700394402 40.0 37.0 40.0 34.0 41.0 33 38.36291926983351 40.0 37.0 40.0 35.0 41.0 34 38.465603294823914 40.0 37.0 40.0 35.0 41.0 35 38.60168929531255 40.0 38.0 40.0 35.0 41.0 36 38.66297860458623 40.0 38.0 40.0 36.0 41.0 37 38.70660709922865 40.0 38.0 40.0 36.0 41.0 38 38.59435970821263 40.0 38.0 40.0 35.0 41.0 39 38.64388677533071 40.0 38.0 40.0 36.0 41.0 40 38.65631217060486 40.0 38.0 40.0 35.0 41.0 41 38.61495235768385 40.0 38.0 40.0 35.0 41.0 42 38.606924714669645 40.0 38.0 40.0 35.0 41.0 43 38.61648808069526 40.0 38.0 40.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4640499080779392 1101 2 0.11418679386952135 1101 3 0.007743799767936821 1101 4 0.9042685351575983 1101 5 0.59434810349061 1101 6 0.7808641422577907 1101 7 0.1975499141157826 1101 8 0.6066006684732201 1101 9 0.19083123509141586 1101 10 0.5613263053073183 1101 11 0.33726935258242463 1101 12 0.43957567547231946 1101 13 0.4794621857447652 1101 14 0.11721096632007644 1101 15 0.5649313014594242 1101 16 -0.015264318916347719 1101 17 0.35529371127522325 1101 18 -0.6500716203484131 1101 19 -0.4304993184059924 1101 20 0.5054199406874886 1101 21 0.6136900927186275 1101 22 1.3615677990157025 1101 23 -0.17823379706575793 1101 24 -1.063575288793288 1101 25 -0.8537822627722846 1101 26 0.6101388127886054 1101 27 0.36604988204842215 1101 28 0.8091873914020127 1101 29 0.827236170010508 1101 30 0.7235071995212863 1101 31 0.6109996018727202 1101 32 0.46252105665263343 1101 33 0.9201796424216226 1101 34 0.3455713962994764 1101 35 -0.17303340140310297 1101 36 0.20492114916301318 1101 37 -0.059761435360279336 1101 38 0.38787452890358765 1101 39 0.45362299806164685 1101 40 0.4097948410205845 1101 41 -0.25509690097376136 1101 42 0.37916159050386256 1101 43 0.2684496359455224 1102 1 -0.19749800346996693 1102 2 -0.09772966804693795 1102 3 -0.541396249372113 1102 4 -0.3174759365868738 1102 5 -0.49164698250447714 1102 6 0.1076454690391202 1102 7 0.14226735883323016 1102 8 0.40451221638476653 1102 9 -0.028456234196056585 1102 10 0.27877102275203214 1102 11 0.28505805037112 1102 12 0.5243422602389032 1102 13 -0.2170980108154339 1102 14 0.06438541349452365 1102 15 0.18962417615229654 1102 16 -0.03184908550111487 1102 17 -0.5765244705429566 1102 18 0.08395786368107139 1102 19 0.7568471189404491 1102 20 1.0686877539553024 1102 21 0.12228960131813693 1102 22 0.9653761528240565 1102 23 0.3770487582167945 1102 24 0.7349505097325064 1102 25 -0.4324063413963657 1102 26 -0.30755160490181765 1102 27 -0.6609771449786024 1102 28 0.17589500810962733 1102 29 -0.6205525277781874 1102 30 0.2173646933787836 1102 31 -0.4848234939503726 1102 32 -0.3777737836422048 1102 33 0.42693639917838055 1102 34 -0.21953916881108881 1102 35 0.26799853962883446 1102 36 0.27740272164458446 1102 37 -0.4485820741809121 1102 38 -0.16187977085071026 1102 39 0.42352471796336744 1102 40 0.0885417697675166 1102 41 0.12635273047587248 1102 42 0.3932893546316265 1102 43 0.5411769086727958 1103 1 -0.3819588914192309 1103 2 -0.5401186743894328 1103 3 -0.44731591110573277 1103 4 0.07523019449135404 1103 5 0.17008663271751345 1103 6 -0.16508180368815317 1103 7 -4.38772244997665E-4 1103 8 -0.012506811099378012 1103 9 0.30082494973629537 1103 10 0.10752366545816727 1103 11 -8.827530115453897E-4 1103 12 0.0687397232410234 1103 13 -0.10821851821501127 1103 14 0.11406828875879427 1103 15 0.6742964805920444 1103 16 0.42533907517541536 1103 17 1.169247199647316 1103 18 0.36249908989671553 1103 19 0.9032530386021804 1103 20 0.2320069928559363 1103 21 0.10960461188896176 1103 22 0.140323298701432 1103 23 0.4383595404578102 1103 24 0.35440082685724406 1103 25 0.0739361533182219 1103 26 0.46306150292059556 1103 27 0.5018135526958147 1103 28 -0.3309760278311771 1103 29 0.8165510663021465 1103 30 0.25964799147603657 1103 31 -0.05776218316813697 1103 32 -0.08179069696144126 1103 33 -0.06724964733324867 1103 34 0.019361465438379355 1103 35 0.24368564322291775 1103 36 -6.099633448428676E-4 1103 37 0.28027627677045786 1103 38 0.45387075769051677 1103 39 0.5900152042212952 1103 40 0.4801908183933037 1103 41 0.4249785232877059 1103 42 0.44138660621725023 1103 43 0.4312403336199395 1104 1 0.19773481249455216 1104 2 -0.8798804441001522 1104 3 -0.1722166484851968 1104 4 0.06445310997631992 1104 5 0.39527097758421803 1104 6 -0.23825253539546765 1104 7 -0.33112510236411197 1104 8 -0.5904988700897391 1104 9 0.397818710371574 1104 10 0.2765537278518124 1104 11 0.21465893728908014 1104 12 0.5054952535870214 1104 13 0.003522831756633593 1104 14 -0.3646611496983816 1104 15 0.12920289012125608 1104 16 -0.9592326775188553 1104 17 -0.23007214238331386 1104 18 -0.28355876603068 1104 19 0.33944135397370445 1104 20 -0.17355171389392154 1104 21 0.862866097992196 1104 22 0.71094156302361 1104 23 0.1309290242921861 1104 24 -0.1453155656555154 1104 25 0.30706150782758357 1104 26 0.019499392880888422 1104 27 -0.7097576327834787 1104 28 0.4835446755153967 1104 29 -0.1964948781107836 1104 30 -1.8447195638274252 1104 31 -0.628947662464789 1104 32 -1.0806562670124933 1104 33 -0.37184408862649576 1104 34 -0.321534734221288 1104 35 -0.2719127685751559 1104 36 -0.2802469457611849 1104 37 -0.2529057992363448 1104 38 -0.3220793273917266 1104 39 -0.3209320447860762 1104 40 -0.06944049187328716 1104 41 -0.5926051409210231 1104 42 -0.2846485611111689 1104 43 0.3172301237503987 1105 1 0.15985209324183103 1105 2 0.4781890940420368 1105 3 0.39187260942865976 1105 4 0.2429689376877917 1105 5 0.45806288209900714 1105 6 0.047684153758652315 1105 7 0.12969482498409945 1105 8 0.12356444075614093 1105 9 0.4507507290534605 1105 10 0.19559887575010038 1105 11 0.36774663837886123 1105 12 0.37057050008417036 1105 13 -0.2606183202931902 1105 14 0.28972390479045984 1105 15 -0.03426363816105038 1105 16 0.6069323458335063 1105 17 0.03614477510500791 1105 18 0.27689790236578915 1105 19 0.6741585309327078 1105 20 -0.08169909324005786 1105 21 1.0081696786875796 1105 22 -0.11054260926491821 1105 23 0.7406851218531614 1105 24 1.0560336818795122 1105 25 1.0501855348125346 1105 26 0.8752336949047645 1105 27 0.5730654082128837 1105 28 0.7160304046280004 1105 29 -0.35217728599869247 1105 30 -0.8348596779945225 1105 31 0.04515716368985778 1105 32 0.2141024252552839 1105 33 0.37906405875284577 1105 34 0.23113781378078357 1105 35 0.4411126595514645 1105 36 0.061735410232593324 1105 37 0.6785939412929736 1105 38 0.5736134593233686 1105 39 0.4771997663192664 1105 40 0.6306114022626801 1105 41 0.09395427786465405 1105 42 0.005958600279591053 1105 43 0.5450453806263766 1106 1 -0.14372860213958916 1106 2 -0.07500239531966457 1106 3 -0.342948355802271 1106 4 0.23518481729339413 1106 5 0.4684417092915325 1106 6 0.42028404997038393 1106 7 0.20546026348954882 1106 8 -0.2490354554555907 1106 9 -0.16315689938452493 1106 10 -0.11369017458721231 1106 11 -0.4682678919792096 1106 12 -0.2506023073885899 1106 13 0.02791307565907175 1106 14 -0.4378318814056996 1106 15 -0.06591906109825629 1106 16 0.40662098101773125 1106 17 -0.13251116677355412 1106 18 0.15546562421322108 1106 19 -0.660558646026292 1106 20 0.3142531641548558 1106 21 -1.3078656093248782 1106 22 -0.728082827220291 1106 23 -0.2593148781468386 1106 24 -0.950193614436003 1106 25 0.1119395566080712 1106 26 1.0438896367833266 1106 27 -0.44146494985665186 1106 28 -0.12621142204557856 1106 29 0.462041707255068 1106 30 0.47235360690427797 1106 31 -0.5313866868550292 1106 32 -0.03187577920761697 1106 33 -0.7864782349679587 1106 34 -0.3703152220261643 1106 35 -0.149961549062958 1106 36 -0.060734750639227286 1106 37 -0.7894911650900056 1106 38 -0.224518351781974 1106 39 -0.22337106917631644 1106 40 -0.24017219919035426 1106 41 0.35861437127409346 1106 42 0.03242460962054139 1106 43 0.29283987984796056 1107 1 0.17618064346449103 1107 2 0.43662551165707697 1107 3 0.18059106563845972 1107 4 -0.4596535264388777 1107 5 -0.41130871611969866 1107 6 0.5325926149164957 1107 7 0.4181658789177902 1107 8 -0.15204169482031205 1107 9 -0.21080295724044973 1107 10 -0.008755404309276571 1107 11 -0.14041763673247232 1107 12 -0.21033004420083756 1107 13 0.05457217945941295 1107 14 -0.07197822286911304 1107 15 0.39522671315018343 1107 16 0.8439437263382068 1107 17 -0.06331093988756464 1107 18 -0.3584311426614235 1107 19 0.5776716432533462 1107 20 -0.0935744024929015 1107 21 0.57472089095873 1107 22 -0.16200228269391204 1107 23 -0.4686172037282361 1107 24 -0.43629684756135845 1107 25 0.4227733626205463 1107 26 0.32352661919966863 1107 27 1.0832089015330268 1107 28 1.0411169954246375 1107 29 1.188644089557961 1107 30 1.1433689217085927 1107 31 0.8492145610179094 1107 32 0.7321627914106514 1107 33 0.07228523638768536 1107 34 -0.3062199299104549 1107 35 -0.291198077707314 1107 36 -6.099633448428676E-4 1107 37 -0.09181674648535676 1107 38 0.15154517629516562 1107 39 0.3109454367794342 1107 40 -0.24073941416483535 1107 41 -0.27269589531694294 1107 42 -0.39582269610833265 1107 43 -0.03387594544982875 1108 1 0.0366457597435641 1108 2 -0.028490767412691298 1108 3 0.36663757726636703 1108 4 0.42406740379367847 1108 5 0.8677610513221623 1108 6 0.6721274986374297 1108 7 0.4879333207782608 1108 8 0.708423421458761 1108 9 0.18454587996885152 1108 10 -0.12503447407672041 1108 11 -0.21018507859293578 1108 12 0.34780949068287725 1108 13 0.24061869108731315 1108 14 0.20709154457274792 1108 15 -0.04663375196609465 1108 16 -0.039777203894349356 1108 17 0.052968129879872095 1108 18 -0.6375009101032845 1108 19 -0.7944213800024684 1108 20 0.30177443471639975 1108 21 -0.05318608578545536 1108 22 -0.8364208873450778 1108 23 -0.0965241804724215 1108 24 0.19161012918282694 1108 25 0.2832384788996194 1108 26 0.532828944781059 1108 27 0.7343716922307024 1108 28 -0.35423184178466016 1108 29 -0.2299605616048268 1108 30 -0.7868636364309438 1108 31 -0.5461342761913883 1108 32 -0.45388372021725587 1108 33 -1.0672496473332487 1108 34 -0.7713362089802231 1108 35 -0.5935236591026651 1108 36 -0.3727029866006575 1108 37 -0.04530511857837638 1108 38 -0.03450133533274169 1108 39 0.008619855384090158 1108 40 -0.10120453044390132 1108 41 0.35521108142724245 1108 42 0.2553400945893429 1108 43 0.2451938219920322 1109 1 0.3706838147118461 1109 2 0.49317942286215555 1109 3 0.29951284153697344 1109 4 0.13706951795858657 1109 5 0.28108029022279624 1109 6 0.6303727417663936 1109 7 0.5513582679241367 1109 8 0.6317849436574932 1109 9 0.4942710385312168 1109 10 0.6651346591156724 1109 11 0.1941489594620336 1109 12 0.22888771478436354 1109 13 0.6238110800936596 1109 14 0.24620359531270353 1109 15 0.21235144887956992 1109 16 0.17269636904433838 1109 17 -0.5537971978156833 1109 18 -0.029678499955288373 1109 19 0.07502893712226921 1109 20 0.6368695721371225 1109 21 -1.309528580500043 1109 22 0.55628524373315 1109 23 0.3770487582167945 1109 24 -0.4241403993584001 1109 25 0.09032093133090768 1109 26 -0.3302788776290839 1109 27 0.4299319459304911 1109 28 -0.11955953734491942 1109 29 0.26581110858544577 1109 30 -0.009908033893943013 1109 31 0.03790377877689366 1109 32 0.23586257999416205 1109 33 0.1314818537238338 1109 34 0.16682446755254432 1109 35 -0.14109236946207204 1109 36 -0.3589609147190558 1109 37 0.14232701672817427 1109 38 0.542665683694743 1109 39 0.40079744523609406 1109 40 0.4067235879493296 1109 41 0.48998909411223224 1109 42 0.18874390008617326 1109 43 -0.004277636781750971 1110 1 -0.5256798216517851 1110 2 -0.5750023953196646 1110 3 -0.9668507948266623 1110 4 -0.6038395729505055 1110 5 -0.14619243704993323 1110 6 -0.6850818036881563 1110 7 -0.3613690048031373 1110 8 -0.523669601797053 1110 9 -0.44754714328696465 1110 10 -0.37759261361160057 1110 11 -0.37948740417433413 1110 12 -0.6047486488520022 1110 13 -0.6189161926336126 1110 14 0.06802177713088753 1110 15 -0.11128491475679425 1110 16 -0.3909399945920242 1110 17 0.05529371127522609 1110 18 -0.07331486359165496 1110 19 -0.038607426514097654 1110 20 -0.03403951877196931 1110 21 1.487744146772684 1110 22 -2.1173511199032156 1110 23 -0.5393148781468398 1110 24 0.6985868733688747 1110 25 0.05486638587635895 1110 26 -0.08391524126545136 1110 27 0.13902285502139478 1110 28 0.5913495535641715 1110 29 0.2839929267672687 1110 30 0.11918287519696236 1110 31 0.11154014241326138 1110 32 0.49495348908507 1110 33 0.516936399178384 1110 34 0.9077335584616364 1110 35 -0.006546914916619073 1110 36 -0.08805182380996257 1110 37 -0.4494911650900022 1110 38 -0.419152498123438 1110 39 -0.2555661911275422 1110 40 -0.6123673211415763 1110 41 -1.1145563604332196 1110 42 -1.494892463550194 1110 43 -0.6515503640544793 1111 1 -1.0434575994295656 1111 2 -0.4083357286530003 1111 3 -0.8535174614933254 1111 4 -0.7371729062838384 1111 5 -0.7906368814943718 1111 6 -0.6539706925770403 1111 7 -0.3435912270253567 1111 8 -0.3303362684637179 1111 9 -0.3875471432869624 1111 10 -0.13537039138937956 1111 11 -0.375042959729889 1111 12 -0.7291930932964448 1111 13 -0.25447174818916807 1111 14 0.21691066601977482 1111 15 -0.07350713697901412 1111 16 0.2646155609635308 1111 17 0.35529371127522325 1111 18 -0.048870419147213795 1111 19 1.416948129041458 1111 20 0.7792938145613633 1111 21 1.1788552578837965 1111 22 1.8648711023190074 1111 23 0.7629073440753871 1111 24 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2057.0 34 3795.0 35 5931.0 36 6615.0 37 4252.0 38 3797.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.17102369899828976 0.6073086454224983 0.28620292485428084 98.93546473072493 2 98.03497260130536 0.9319046455621096 0.7469198282782451 0.28620292485428084 3 0.631740602422254 0.8376670971344804 98.20948657987505 0.3211057205682175 4 0.2547904087117378 98.62133956929951 0.3350668388537922 0.7888031831349691 5 0.537503053994625 98.66322292415623 0.43977522599560226 0.3594987958535479 6 98.18156434330389 0.5549544518515933 0.42930438728142123 0.8341768175630868 7 0.4711877421381453 0.37345991413912255 0.7992740218491501 98.35607832187358 8 0.9074726885623539 1.5496841296987889 96.40501204146452 1.137831140274336 9 30.231421690111347 20.684840657614576 27.672868162937625 21.410869489336452 10 28.543506334857423 20.624061987365188 26.13870371016718 24.693727967610204 11 28.310119733305406 19.621600865710196 26.78814535553461 25.28013404544979 12 25.860383944153575 20.57940663176265 28.516579406631763 25.043630017452006 13 20.13612565445026 23.158813263525303 30.153577661431065 26.55148342059337 14 52.37197612315426 26.23311341501728 9.64847977100569 11.746430690822773 15 25.691947925028796 24.131792956615826 37.51701511291054 12.659244005444837 16 13.078534031413614 16.164048865619545 57.326352530541016 13.43106457242583 17 12.645282887159262 14.666154758996194 57.64196712156644 15.046595232278106 18 16.024432809773124 19.965095986038396 13.507853403141363 50.50261780104712 19 38.638505847442836 29.753883749345434 13.154128120090766 18.453482283120966 20 24.157068062827225 30.240837696335078 23.99301919720768 21.609075043630018 21 21.618791665212385 26.871662420159854 24.794946075180622 26.71459983944714 22 25.130012914034417 27.025234721301178 25.0811490000349 22.763603364629507 23 22.66936581620188 28.683117517713168 26.969390248158874 21.678126417926073 24 22.655404697916303 26.58894977487697 28.3340895605738 22.421555966632926 25 20.32040766465394 25.311507451746884 28.257303410003143 26.110781473596035 26 21.384942933929008 26.60291089316254 28.355031238002166 23.657114934906286 27 23.21384942933929 26.288785731737114 27.478971065582353 23.018393773341245 28 21.667655579211896 27.76517399043663 27.370772398869146 23.19639803148232 29 24.48780147289798 27.332379323583815 26.13870371016718 22.04111549335102 30 22.610031063488183 25.64308401102928 28.969320442567447 22.777564482915082 31 21.96781962235175 26.15964538759555 27.51387386129629 24.358661128756413 32 24.39007364489896 26.12125231231022 26.564517817877213 22.924156224913617 33 22.096959966493316 27.00080276430142 30.17695717426966 20.725280094935606 34 21.238351191930473 29.68133747513176 29.09148022756623 19.988831105371542 35 20.938187148790618 30.02338487312834 26.194548183309486 22.84387979477156 36 21.412865170500154 27.66744616243761 29.684827754703154 21.23486091235908 37 26.16147160459353 27.662396593249326 26.31505462668854 19.8610771754686 38 21.48965132107082 27.768664270008024 29.40211510942026 21.33956929950089 39 21.590869428641234 29.964050120414644 27.1788070224425 21.266273428501623 40 24.381304757583163 27.73220705783797 27.840413277950365 20.0460749066285 41 22.097731239092496 30.62129144851658 27.57417102966841 19.706806282722514 42 21.765383407210916 30.895954765976757 27.328889044012428 20.009772782799903 43 20.679906460507485 27.890824055006807 28.798296743569157 22.630972740916548 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.0 9 6.0 10 8.5 11 11.0 12 11.0 13 17.0 14 23.0 15 32.5 16 42.0 17 62.5 18 83.0 19 83.0 20 119.5 21 156.0 22 177.0 23 198.0 24 290.0 25 382.0 26 382.0 27 440.0 28 498.0 29 559.0 30 620.0 31 846.5 32 1073.0 33 1073.0 34 1126.0 35 1179.0 36 1311.5 37 1444.0 38 1632.5 39 1821.0 40 1821.0 41 1978.5 42 2136.0 43 2287.5 44 2439.0 45 2517.5 46 2596.0 47 2596.0 48 2612.0 49 2628.0 50 2681.0 51 2734.0 52 2631.5 53 2529.0 54 2529.0 55 2221.0 56 1913.0 57 1713.0 58 1513.0 59 1286.0 60 1059.0 61 1059.0 62 868.5 63 678.0 64 532.0 65 386.0 66 264.0 67 142.0 68 142.0 69 129.5 70 117.0 71 106.5 72 96.0 73 63.0 74 30.0 75 30.0 76 32.0 77 34.0 78 50.5 79 67.0 80 40.5 81 14.0 82 14.0 83 9.0 84 4.0 85 2.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.006980559142787337 10 0.0 11 0.013961118285574675 12 0.0034902795713936687 13 0.0034902795713936687 14 0.013961118285574675 15 0.0 16 0.0034902795713936687 17 0.0 18 0.0034902795713936687 19 0.02094167742836201 20 0.0034902795713936687 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.006980559142787337 38 0.0 39 0.0 40 0.006980559142787337 41 0.0034902795713936687 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 28651.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.83222226100311 #Duplication Level Percentage of deduplicated Percentage of total 1 74.60541669225572 42.399916233290284 2 12.841613953202726 14.596349167568324 3 4.85168580728367 8.271962584202996 4 2.3152981637290426 5.263341593661652 5 1.2589817601179145 3.5775365606785106 6 0.9519130381379353 3.2459600013961114 7 0.6878339372351532 2.736379183972636 8 0.4360375852115703 1.9824787965516038 9 0.3869065896947737 1.97898851698021 >10 1.633605600933489 14.749921468709642 >50 0.024565497758398332 0.8446476562772679 >100 0.006141374439599583 0.3525182367107605 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTAAGCTGCGACAATTAGGGCAGGGGCAAGTGTAGGGGCAGC 101 0.3525182367107605 No Hit CGTAAGCTCGATCGTTCCGACAAGTGCAGCTGCTGTGCCTGAA 74 0.2582806882831315 No Hit CGTAAGCTCCAACGTTTCAGAAAGGCCTGAATGAGCTCTTGAA 62 0.21639733342640743 No Hit CGTAAGCTGTGCGAATTCGAAGCTTTCTTCAAAAAGCTCTCAC 54 0.18847509685525812 No Hit CGGCAGCTTCGCGGCCACGCCGTGTCGGCGCCGAACACCGGCG 52 0.18149453771247076 No Hit CGTAAGCTCGTCTGTTGCCTAATGGTGGCCTTTCTCATCCTCT 45 0.1570625807127151 No Hit CTTAAGCTATCGTAGTCCGACGATCTTCGTCGTACTGTAGAAC 44 0.15357230114132142 No Hit CGTAAGCTTTGTTGATTGGGCTACATCCATCTGTCATCTGAAG 42 0.1465917419985341 No Hit CGTAAGCTCGTGCGTTTGGCGTGGCTGAGCCAAGAAAGAAGGC 41 0.14310146242714042 No Hit CGTAAGCTTGCCTAGTCCGACGATCAGTGCCGTAAGCTTTTTT 41 0.14310146242714042 No Hit CGTAAGCTTTGATGGGTTACGAGCCTACATTCAAGGAACTGAA 40 0.13961118285574675 No Hit CGTAAGCTATTATGTTTCTAACATAGTTCATTCGCAGGGTTGT 38 0.13263062371295942 No Hit CGTAAGCTCTGAAATTCCGACGATCACCGTCGTAATGTAGAAC 36 0.12565006457017205 No Hit CGTAAGCTATGCGGGTCCGACGATCGTCGTCGTACTGTAGAAC 36 0.12565006457017205 No Hit CGTAAGCAGAAGTAATACGACTCACTATAGTGAGTCGTATTAC 35 0.1221597849987784 No Hit CGTAAGCTGATCCGTTGCGTTCTTGATCTTGTTTGCCATGTAA 35 0.1221597849987784 No Hit CGTAAGCTGCATAAATTCGGGAAAAGCTGGCCAAAATGTACAA 33 0.11517922585599107 No Hit CGTAAGCTTATTCGGTTTGTATCTTAGGAGCAATAGGCTCTTC 31 0.10819866671320372 No Hit CGTAAGCTAAACTGCTTCGGAGGTCGTGGGTGACAAGTTGGTG 31 0.10819866671320372 No Hit CGTAAGCTACAGCAGCTCAGGTGAACCCAGTAATAATTTTATA 31 0.10819866671320372 No Hit CGTAAGCTAGTTTAAGTGCTGATGTGTATTACAACGCAAGCTT 30 0.10470838714181005 No Hit CGTAAGCTACAACGGTCCGACGATCATTGTCGTACTGTAGAAC 30 0.10470838714181005 No Hit CGTAAGCTGCTCCGTTTAGATCTAAAAAGTCCCACCTCAGTTT 29 0.10121810757041638 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.02792223657114935 0.0 0.0 16 0.0 0.0 0.038393075285330355 0.0 0.0 17 0.0 0.0 0.038393075285330355 0.0 0.0 18 0.0 0.0 0.04188335485672402 0.0 0.0 19 0.0 0.0 0.04537363442811769 0.0 0.0 20 0.0 0.0 0.05933475271369237 0.0 0.0 21 0.0 0.0 0.08376670971344805 0.0 0.0 22 0.0 0.0 0.08376670971344805 0.0 0.0 23 0.0 0.0 0.1465917419985341 0.0 0.0 24 0.0 0.0 0.1465917419985341 0.0 0.0 25 0.0 0.0 0.23733901085476947 0.0 0.0 26 0.0 0.0 0.32808627971100485 0.0 0.0 27 0.0 0.0 0.3664793549963352 0.0 0.0 28 0.0 0.0 0.42930438728142123 0.0 0.0 29 0.0 0.0 0.5514641722801996 0.0 0.0 30 0.0 0.0 0.6596628389934034 0.0 0.0 31 0.0 0.0 0.684094795993159 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCTTGC 55 1.0913936E-11 37.0 5 AGCTCCT 55 1.0913936E-11 37.0 5 AGCTCCG 40 3.6150595E-8 37.0 5 AGCTTCC 35 5.3802796E-7 37.0 5 AGCTTAG 80 0.0 37.0 5 TAAGCTG 920 0.0 37.0 3 TAAGCTC 550 0.0 37.0 3 TAAGCTA 440 0.0 37.0 3 ATTAGGG 20 0.0018121459 37.0 15 AGCTATC 25 1.2038397E-4 37.0 5 AGCTAGC 35 5.3802796E-7 37.0 5 AGCTAGA 20 0.0018121459 37.0 5 GCTGGTA 20 0.0018121459 37.0 6 AGCTACT 55 1.0913936E-11 37.0 5 AGCTAAG 25 1.2038397E-4 37.0 5 AGCTAAA 20 0.0018121459 37.0 5 GCTGGCG 30 8.032481E-6 37.0 6 TGTCATG 20 0.0018121459 37.0 8 AATTAGG 25 1.2038397E-4 37.0 14 GAAGATT 20 0.0018121459 37.0 11 >>END_MODULE