FastQCFastQC Report
Thu 26 May 2016
SRR2099202_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099202_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128178
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTATGGATGGGCGTTGGGTGGAGGAGAAGGACTCTCCTG59520.5275763221761105No Hit
ATCGGTACACCCAACCGCGACGCGGCGCGTGCGCGGGCGGGGC27170.24083079088583537No Hit
ATCGGTACACGTTGGGCGGATCACGAGGTCAAGAGATCGAGAC22020.19518196596636347No Hit
ATCGGTATGGATGGGGCGTTGGGTGGAGGAGAAGGACTCTCCT15670.13889652164817962No Hit
ATCGGCGCGGTAGGAGATCAGCACGACGCCATCGGGCGATTCA14810.13127361107910276No Hit
AACGGCACATTGCGCGGGTGCGGCGTAGCGGAGCTTTGCTCAC13000.11523004348604564No Hit
ATCGGTCAAGCGCCTCGGTGCGCAGCGAAGGGCAGGGCGGCGC11610.10290929268253768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG308.2728275E-637.025399
CGTTGCG850.037.0171748
ACGCGGA750.037.0171747
TCGCGCA308.3056275E-637.00403610
GGTACGG77500.036.892854
CGGTACG238950.036.8866963
CGGTACA237500.036.8781433
GGTACGT65400.036.8707664
GTACCGG28400.036.818395
CGGTACC284600.036.7960323
TACACCG6100.036.7121286
GGTACAC71400.036.649384
TACCGCG10050.036.6472176
GTACCGT19500.036.634265
GGTACGC66800.036.596694
GTACGGG17000.036.5784345
GTACGCG16900.036.5758555
GGTACCG92800.036.5735244
GGTACTC61300.036.5594024
GGTACCC82300.036.517554
Produced by FastQC (version 0.11.5)