FastQCFastQC Report
Thu 26 May 2016
SRR2099201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24169
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTATGGATGGGCGTTGGGTGGAGGAGAAGGACTCTCCTG1520.628904795399065No Hit
ATCGGTACCGCCAGTTCCATGACCAAGATCTATGGAGGACGGC1500.6206297323017088No Hit
ATCGGTACGGTTCATTTAGCTCCCAAGCCATAATGCCGAACAG1490.6164922007530308No Hit
ATCGGTACACTTAGTTTCCTTAGGAGCTTGGGCACAAAGGCAA1120.4634035334519426No Hit
ATCGGTACACAGCGATTCAGTCTTAAGTTCTTAGATTTTTTTT790.32686499234556665No Hit
ATCGGTACTCATCGATTCTCCACCCTGGAGATCTGTGCTCCTG620.2565269560180396No Hit
ATCGGTACACGTTGGGCGGATCACGAGGTCAAGAGATCGAGAC590.24411436137200546No Hit
ATCGGTACACCCAACCGCGACGCGGCGCGTGCGCGGGCGGGGC520.21515164053125904No Hit
AACGGCACATTGCGCGGGTGCGGCGTAGCGGAGCTTTGCTCAC510.211014108982581No Hit
TTCGCTACCTGGGCTCGACACCGCCAGTCTCCCAGGCCCGCAC510.211014108982581No Hit
ATCGGTACCGCCAGGTTGGTTCCATGACCAAGATCTATGGAGG470.19446398278786878No Hit
ATCGGTACCCCAGGTTTCAGCCTCAACCCAGGGGAATCGTTAC460.1903264512391907No Hit
ATCGGTACTTTCAAGTCCGACGATCCAAGGATCGTCGGACTGT430.17791385659315653No Hit
ATCGGCGCGGTAGGAGATCAGCACGACGCCATCGGGCGATTCA390.1613637303984443No Hit
ATCGGTACCTACGGTTTCTGAAGATGTAGCAGAAACTGTCGTT350.14481360420373204No Hit
ATCGGTACATACCCATGCACACACAAACCTGGTTTATGTGGCG340.140676072655054No Hit
ATCGGTCAAGCGCCTCGGTGCGCAGCGAAGGGCAGGGCGGCGC320.13240100955769787No Hit
ATCGGTATGGATGGGGCGTTGGGTGGAGGAGAAGGACTCTCCT300.12412594646034175No Hit
ATCGGTACGGGGTAGTCCGACGATCATTGCATCGGACTGTAGA290.1199884149116637No Hit
ATCGGTACCTAAGGATCCGCCCACACCCACGTGTCCCTGTCCA290.1199884149116637No Hit
ATCGGTACGGTTTCTTTCGACAACGAAAGTCATATCGAAGAAA290.1199884149116637No Hit
ATCGGTACCCGTCAATTTGAGGGAGAGCGTCATATTGAAGGAA290.1199884149116637No Hit
ATCGGTACGCGGGGCTTAGCACCGTGGCTAGGCTGGGAAGTAA280.11585088336298564No Hit
ATCGGTACACTATAATTTGTGAAAGCTTTGGATTCAGGTGATG260.10757582026562952No Hit
ATCGGTACTGGCTGGTCCGACGATCTCGTCATCGGACTGTAGA260.10757582026562952No Hit
ATCGGTACACTACGTTTCTCCTTACTCATCCAATTGGTACCCA250.10343828871695146No Hit
ATCGGTACCGAGCGGTTCAGAGTACGTTCACAGCCTTCTTACA250.10343828871695146No Hit
ATCGGTACTTATTGCTTCAGGACACCTCCCTGAAGCTGGAAAA250.10343828871695146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACTA200.001791566337.0622416
GTTCCAT251.1863396E-437.0622419
TAGCTCC307.887731E-637.0622417
CATTTTG200.001791566337.0622413
TTTCACT200.001791566337.0622415
GGTTCGA251.1863396E-437.0622414
GTTTCAA251.1863396E-437.0622414
TTCCTTA251.1863396E-437.0622416
TATTTCA200.001791566337.0622413
CACTAAT200.001791566337.0622418
TCACTAA200.001791566337.0622417
GCTCCCA307.887731E-637.0622419
TGTATCG200.001791566337.0622413
TTAGCTC307.887731E-637.0622416
ATTTCAC200.001791566337.0622414
AGTTTCC251.1863396E-437.0622413
ATTTAGC355.262955E-737.0622414
GTACGGG452.410161E-936.985515
TACAGGC200.001809969536.9855086
TACAGCG200.001809969536.9855086
Produced by FastQC (version 0.11.5)