FastQCFastQC Report
Thu 26 May 2016
SRR2099199_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099199_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45958
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACAGTCTGTTAGTTCGAGGAGGAGGAGGGTGAAGATGAGG4330.9421645850559206No Hit
TCGACAGTACACCGCGTCGGCGGCTCGGACTCCGATCTGAACG1650.35902345619913834No Hit
TCAACAGGCTGGCGCGGTGCCGCCCGCGCCGGTGATCGAGCGG1350.2937464641629314No Hit
GCTACAGTGTGGTGTTTCACTAGATCGTGTTATGGACGATGAC1240.2698115670829888No Hit
GCTACAGTCATATGTTGCAGTGAGCCGAGATCATGCCACTGCA1230.2676356673484486No Hit
GCTACAGTGCGATTTCGAAGCGGGATCTCGCCCACCATTTGGG1140.2480525697375865No Hit
GCTACAGTCCCTTGTTTGGTTTGCATGACTCTTTCCTCAATGA1030.22411767265764393No Hit
GCTACAGTCTGATGTTTACTTTGAAAAAATTAGAGTGTTCAAA920.20018277557770137No Hit
GCTACAGTTGCTTGGTTCGATCAGGCTACTACAAAGTTCTGGG810.17624787849775883No Hit
TCGACAGTTGGTTGTTTTGGGCAAAATTGAGAGAGCCATCGGC780.16972017929413813No Hit
GCTACAGTTGGCTGATTAGTTTAATTAAACTAACGAATAGTTT730.15884068062143697No Hit
TCGACAGTAGTCAGGTTCCTGTTGATCTTGACCTTCTCTTTGG700.15231298141781627No Hit
GCTACAGTCAAATGCTTCGGAGGTCGTGGGTGACAAGTTGGTG680.1479611819487358No Hit
GCTACAGTGGGATGTTGCAGAAAAGTCATTGCATGGAAAGATG660.14360938247965532No Hit
GCTACAGTCATATGTTTACTTTGAAAAAATTAGAGTGTTCAAA650.1414334827451151No Hit
TCGACAGTAATGCGATTCATGAATGGGTTCCACCTTCGACACC630.13708168327603462No Hit
GCTACAGTATCGTGTTTCAGTCTCGACGGGCTAGCACACATCT550.11967448539971279No Hit
GCTACAGTGGGCTGATTCCCCATGGCTAAGATCTCACACTGCT540.11749858566517254No Hit
GCTACAGTGGGTTGGAGCGGACTTGTAAGGACCGCCGCTTTCG530.11532268593063232No Hit
GCTACAGTTGCTTGGGGCGCATTGCGATCCAACCATCGGCAGC500.10879498672701161No Hit
GCTACAGTTGTATGCGACGATCGGACTGTAGAACTCTGAACCC500.10879498672701161Illumina DpnII expression Adapter 1 (95% over 23bp)
GCTACAGTGTGCTGTTTACTTTGAAAAAATTAGAGTGTTCAAA490.1066190869924714No Hit
GCTACAGTTTTGTGTTTACTTTGAAAAAATTAGAGTGTTCAAA480.10444318725793116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG452.4610927E-937.06543413
ATATGTT452.4610927E-937.06543410
TGGTCCG355.4004886E-737.0654313
AATTCTT200.001807815237.0654314
ATGATTC200.001807815237.0654312
GGTCCGA501.6734703E-1037.0654314
TTATTTC251.2026653E-437.0654312
TGATTCC251.2026653E-437.0654313
GTATTTC403.6407073E-837.0654312
TCGTGGT200.001807815237.0654310
TAGTTCG501.6734703E-1037.0654313
TGTTAGT501.6734703E-1037.0654310
CGTGGTT251.2026653E-437.0654311
TCTGTTT355.4004886E-737.0654311
CTAATTA251.2026653E-437.0654312
CGTATTT308.041088E-637.0654311
TAATTAG200.001807815237.0654313
TATTTCA650.037.0654313
GATTTCG200.001807815237.0654311
GCGTATT251.2026653E-437.0654310
Produced by FastQC (version 0.11.5)