FastQCFastQC Report
Thu 26 May 2016
SRR2099197_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099197_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30117
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTCAGGCTTTGGTTGGTGAAGAGATTCTCTCGCGTTTCAG1820.6043098582196101No Hit
CATGTCAGAGCAAGTATGGTAGAATTCACTGTGATTGTACGTG1020.3386791513098914No Hit
CATGTCAGTTGAACGTTCGTGCCCAATGATGGCTGCCTGAACT980.3253976159644055No Hit
CATGCCTGCTCACAGAAAGCAAGGCTGCTTTCTGCTCTTGCTT920.3054753129461766No Hit
CATGTCAGTGATTGTTTCTTCCCTCCTGACCACCATCGGTTGT910.30215492910980507No Hit
CATGCCGGTGCCAGGCCAGGGCGCGATCGTCACCGTCAGTTCG910.30215492910980507No Hit
CATGTCAGAAGGCGTTTTGGATCTGTATATAATTGTTGCTGGT850.2822326260915762No Hit
CATGTCAGCCTTAGATTCTTCAAGTTTACAGTGGGTTTGTTGC660.219145333200518No Hit
CATGTCAGTCACAATTGCACTTTCCGTTCTAAGTGCAATTTCC620.20586379785503206No Hit
CATGTCAGTGTACGTTGGGAAATGCCAGGGCCAGAAGAGGGAG600.19922303018228907No Hit
CATGTCAGTCTGCGATTCTCTCGCGTTTCAGCATATTGAAAGG600.19922303018228907No Hit
CATGTCAGTTTTAGTATCAACCCCTCAAGGGTTGGTGAAGAGA580.1925822625095461No Hit
CATGTCAGGTGTAAGTCCGACGATCAGTCGGACTGTAGAATTC570.18926187867317462No Hit
CATGTCAGTACCAGTTTCATTTCTCATGTTAATGTGAGAGACG530.1759803433276887No Hit
CATGTCAGGTTTAGTTTGGCAGTGCCAGCTAGTCTAGCTAACT510.1693395756549457No Hit
CATGTCAGTTTTCGTTTCTTAATTGGTTGTATTTCTTTTGTTA510.1693395756549457No Hit
CATGTCAGTATCTGTTTCTAGGGTCCAACTACAGGATAGATTA480.15937842414583125No Hit
CATGTCAGCATTGATTTTGAATTGGGAGGCGACAAGAAGAGAA470.15605804030945977No Hit
CATGTCAGGAGCCAATTAAGATTCTTTAACTTTTTTGGAACCT440.1460968888003453No Hit
CATGTCAGTCCAGGTAGCGCCTCGGACGAATACCATAGGGGGT440.1460968888003453No Hit
CATGTCAGACGGCGTTTGGCTGGGGTAGGTTGTCTGAAGGTTT430.14277650496397384No Hit
CATGTCAGTTCTCAATTCTTTGAGGGCTGCCCAGATAAGGAGC430.14277650496397384No Hit
CATGTCAGACATAACTTAGAACGGAAATTGCACTTAGAACGGA430.14277650496397384No Hit
CATGTCAGTCTCGGAAACGCGAGAGAAACCGCGTATCAGCATA420.13945612112760236No Hit
CATGTCAGCACTCGGTTGGTACGCGGTTTCTCTCGCGTTTCAG410.13613573729123085No Hit
CATGTCAGAATATGATTGGATCTGAGGCCTTGTTTTTTCTTTT390.1294949696184879No Hit
CATGTCAGCATTGGTTTTGTTGTGTTCTGTTGAAGGTGCCATT380.1261745857821164No Hit
CATGTCAGTATTAGCTTCGAAGCCAGAAGCCTGTGGTGGTGAA380.1261745857821164No Hit
CATGTCAGCTGTTGATTCAGCCTCATGACTTAGGAAAAGTTGG360.11953381810937344No Hit
CATGTCAGGCTTTGTCTGGACACCGCCGCAGGGGTGGGGTGAG360.11953381810937344No Hit
CATGTCAGTTTTTGATTCTTCCCACTCTGACTTTCAGCTCTTC350.11621343427300194No Hit
CATGTCAGCTTTACAGTCCGACGATCGTTGACATGACTTGGAT340.11289305043663048No Hit
CATGTCAGAGTCAGTTTAGTAACTTTGTGGCAGAAAGCAGTTA330.109572666600259No Hit
CATGTCAGTCAAAATTTCACTAATTCCAATACTTATTATTATT330.109572666600259No Hit
CATGGAAGGCTGCCACGCCGTTCTTGTAAGCGGTGACGTCGAT320.1062522827638875No Hit
CATGTCAGTAGTGGGTGCGCTGGGACATGTCCTCCCTGTACCC320.1062522827638875No Hit
AATGACAGCAGCGGGCCATCGGAGATGGTTCCGTCAAGGGCAA320.1062522827638875No Hit
CATGTCAGAATTTGGTTCAGCATCGCAGACAAGAAGCGCAGAA310.10293189892751603No Hit
CATGTCAGTAGATATTTAGGGACATTTTAAAACCTTGTAAACA310.10293189892751603No Hit
CATGTCAGACGGCGGTTTGGCTGGGGTAGGTTGTCTGAAGGTT310.10293189892751603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCAGC4550.037.0000043
GTCAGGT1450.037.0000044
TTGTTTC355.392467E-737.012
TTTGAGT200.001813610437.010
TTCACTA200.001813610437.016
CAGTCTA200.001813610437.06
CCAATAC200.001813610437.026
CAGTTGG308.046116E-637.06
CAGCATT355.392467E-737.06
CAGTTGC308.046116E-637.06
CAGTCAT308.046116E-637.06
CAGCAGT200.001813610437.06
AGTAGTG200.001813610437.07
GATACGA200.001813610437.014
TCAGGTT700.037.05
TCAGGTG308.046116E-637.05
TCAGGTC355.392467E-737.05
GAGTGAA200.001813610437.026
GGCAATT200.001813610437.07
TCAGGCT800.037.05
Produced by FastQC (version 0.11.5)