FastQCFastQC Report
Thu 26 May 2016
SRR2099196_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099196_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1256632
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAGTCCGGCAGTCCGACGATCGAGTAAGCTAGTCTTTTTT31020.24685031099001142No Hit
AGCAGGTCTCCCCGCGACGGCCTGCGCCAGCGCTGGTTCACGC30570.24326931034702282No Hit
AGCTAGTCTGACTCGTAAGCGAGACTATCTGCGTATTGTAGTA22270.17721974293190051No Hit
AGCTAGTCGGCACGGGAGGGATGGGGAGTCGCCCTCCCCCGGA21650.17228591982378294No Hit
AGCTAGTCAGCCCGGGCTGGGGAGACTCCCAGGTTTTGTCAGT17580.13989775845275307No Hit
AGCTAGTCTCATACAATAACCCACCCCCCCATAGAGGCTGGGG16800.1336906906715729No Hit
AGCAGGTCTCCCCCGACGGCCTGCGCCAGCGCTGGTTCACGCT16030.12756320068245916No Hit
AGCTAGTCGTGAGGGGGAGAGCACTTCGATGGCGATGCCGTAA13330.10607719682452779No Hit
AGCTAGTCTGGCAAGCTAGTCTTTTTTTTTTTTTTTTTTTTTT13260.1055201522800629No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATA200.00184218636.9992411
TAGTCGC72650.036.9120864
CTAGTCA283750.036.8690263
TAGTCGA59300.036.8267254
CTAGTCC324600.036.778763
TAGTCCA77000.036.7255634
TAGTCAC86200.036.6275254
TAGTCCT64450.036.5832564
TAGTCAT52100.036.481154
TAGTCCC92750.036.395424
TAGTCTC75350.036.3753854
TAGTCAA76250.036.285844
TAGTCTA54250.036.127014
TAGTCCG95200.036.0225374
GTCACCG7050.035.9668056
AGTCCCG24650.035.965745
TAGTCAG75600.035.9613384
AGTCGCA22900.035.885415
GCTAGTC1133600.035.7323532
AGTCCGT16950.035.7065965
Produced by FastQC (version 0.11.5)