FastQCFastQC Report
Thu 26 May 2016
SRR2099195_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099195_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35069
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTAGGCGCTACGGAGTGAGGGAGAGGCTTGGGACAGGAAGA1690.4819070974364824No Hit
AGCTAGTCCCCAGGTTTATGGAACCCGGTGGCTCCCGTCATGT1600.4562434058570247No Hit
AGCTAGTCCAAATGGTTCGATCAGGCTACTACAAAGTTCTGGG1150.3279249479597365No Hit
AGCTAGTCCCAGGGTTCCATCCAGTACTAAATGCTTACCGTGT1070.30511277766688527No Hit
AGCTAGTCCCACAGGATCGGCTCCTAATAGGAGGTGAATTAGA820.23382474550172516No Hit
AGCTAGTCACTGGGTTTTGTAAGACAGAGCTTACTGTGTGACC800.22812170292851236No Hit
AGCTAGTCTGAACGTAAAAGAAGCGGATTTAATTCTGCATTTA800.22812170292851236No Hit
AGCTAGTCGAAACGTTTAGTCCCAGCCTTTAAGCATGATCCTG780.22241866035529959No Hit
AGCAGGTCTCCCCGCGACGGCCTGCGCCAGCGCTGGTTCACGC750.21386409649548033No Hit
AGCTAGTCACCCCGGTTCGATCAGGCTACTACAAAGTTCTGGG730.20816105392226755No Hit
AGCTAGTCCTTCTAATTCGACAACGAAAGTCATATCGAAGAAA690.19675496877584192No Hit
AGCTAGTCCAAGGGGTTCGATCAGGCTACTACAAAGTTCTGGG690.19675496877584192No Hit
AGCTAGTCTCATAGTGACATACTTCCTGTTGCTGTACTTCCAC690.19675496877584192No Hit
AGCTAGTCACAGCGTTTACAGTCCTGACTGAAAGTCACCCTGC670.1910519262026291No Hit
AGCTAGTCACTCTGATTAGATAGGGAAAAGATCGGGATGCTAC550.15683367076335225No Hit
AGCTAGTCACACGGTGTGGGCCAGGCACTATTCCAGCAGATTT540.15398214947674585No Hit
AGCTAGTCAAGCCGGTTGGTTGCCAGAAACTCTTAGGTGAGGG530.15113062819013945No Hit
AGCTAGTCGCTGGGTGAAACCCCGTCTCTACTAAAAATACAAA510.14542758561692662No Hit
AGCTAGTCGTCGCGTTTGGCCGTCGCTGTTGGCCACGTGAAGA510.14542758561692662No Hit
AGCTAGTCGGCACGGGAGGGATGGGGAGTCGCCCTCCCCCGGA500.14257606433032022No Hit
AGCTAGTCTGACTCGTAAGCGAGACTATCTGCGTATTGTAGTA480.13687302175710742No Hit
AGCTAGTCCGGCAGTCCGACGATCGAGTAAGCTAGTCTTTTTT450.12831845789728819No Hit
AGCTAGTCTGATAGTTACATTTGGATGGCTTATGTAACCACAA450.12831845789728819No Hit
AGCTAGTCACACTAGTCCGACGATCTAAACAGCTAGTCTTTTT440.12546693661068178No Hit
AGCTAGTCTTGTTGTTTCTGGAACTATCAAGGAGATCCTGGGT440.12546693661068178No Hit
AGCTAGTCTGTCCGATAAGGAAGACCACTTGCCAAAATCTGAG430.1226154153240754No Hit
AGCTAGTCAGACCGTAGATTTTGTCTTTTCAAGATATAATAAG410.11691237275086258No Hit
AGCTAGTCTGATTGTGTCAAAGTCTGAGTGACTGGGTAGTTGA400.11406085146425618No Hit
AGCTAGTCCCACCGGGTCGGTCATGTCTGTATGTTCTTGTTTG400.11406085146425618No Hit
AGCTAGTCTCGGGGTAACTTTTGTGTATGTACCTAGCTGTACT400.11406085146425618No Hit
AGCTAGTCCGGACGGAGCGCCAGGGGCGGAAATTCGCCTGAAA390.11120933017764979No Hit
AGCTAGTCGGTTGGTTTCTTCCTGTCACCCCCATTCTGGAAGG380.10835780889104336No Hit
AGCTAGTCTTCCTGTTCCAGAATGTCTGGTATCTTCACACAGA380.10835780889104336No Hit
AGCGAGTGCCGCCGATTGCAGCAGCAATCGGCGTATACCCTGT360.10265476631783058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCT200.001797061937.0858413
CGTTTAG200.001797061937.0858413
GAGTGAG251.1929255E-437.0858415
AGGATCG251.1929255E-437.0858413
GTTCCAT251.1929255E-437.0858414
GTTCTAC251.1929255E-437.0858415
TGTTTGA200.001797061937.0858413
GGATCGG251.1929255E-437.0858414
GGTTCGA251.1929255E-437.0858414
GGTTTCT251.1929255E-437.0858413
GGTTTAT307.9561105E-637.0858413
TTGGGAA200.001797061937.0858416
TTTAGAT200.001797061937.0858415
CTTCGGT200.001797061937.0858415
GCTTCGG251.1929255E-437.0858414
TTCGGTT200.001797061937.0858416
ATTTAGC307.9561105E-637.0858414
CGATTGG200.001797061937.0858413
GTTCGAT355.328384E-737.0858415
AGGGGTT200.001809758337.0328611
Produced by FastQC (version 0.11.5)