##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099195_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35069 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.548803786820265 37.0 35.0 37.0 33.0 37.0 2 35.72816447574781 37.0 35.0 37.0 35.0 37.0 3 36.06125067723631 37.0 37.0 37.0 35.0 37.0 4 37.844506544241355 39.0 39.0 39.0 35.0 39.0 5 36.7253129544612 37.0 37.0 39.0 34.0 39.0 6 37.354757763266704 39.0 37.0 39.0 35.0 39.0 7 36.68841426901252 39.0 37.0 39.0 33.0 39.0 8 37.4667655194046 39.0 37.0 39.0 35.0 39.0 9 32.16190937865351 33.0 31.0 34.0 31.0 34.0 10 32.419173629131144 34.0 31.0 34.0 31.0 34.0 11 32.41757677721064 34.0 31.0 34.0 31.0 34.0 12 35.897687416236565 37.0 37.0 37.0 35.0 37.0 13 35.87330690923608 37.0 37.0 37.0 35.0 37.0 14 30.788188998830876 31.0 30.0 31.0 30.0 34.0 15 30.299181613390743 31.0 30.0 31.0 27.0 34.0 16 29.47395135304685 30.0 28.0 31.0 25.0 34.0 17 32.38843422966153 35.0 30.0 35.0 26.0 37.0 18 33.48447346659443 35.0 33.0 35.0 28.0 37.0 19 31.413071373577804 35.0 30.0 35.0 25.0 37.0 20 30.779121161139468 33.0 28.0 35.0 17.0 37.0 21 30.805155550486184 33.0 28.0 35.0 17.0 37.0 22 31.77875046337221 34.0 28.0 37.0 17.0 39.0 23 32.23627705380821 34.0 30.0 37.0 23.0 39.0 24 32.66160996891842 35.0 30.0 37.0 25.0 39.0 25 33.031138612449745 35.0 30.0 37.0 25.0 39.0 26 33.373121560352445 35.0 31.0 37.0 26.0 39.0 27 34.669936411075305 36.0 32.0 39.0 27.0 40.0 28 35.23687587327839 36.0 33.0 39.0 27.0 40.0 29 35.7791781915652 37.0 34.0 40.0 30.0 40.0 30 36.23773132966438 37.0 34.0 40.0 31.0 40.0 31 36.720978642105564 38.0 36.0 40.0 32.0 41.0 32 37.17439904188885 38.0 36.0 40.0 32.0 41.0 33 37.51447147052953 39.0 36.0 40.0 33.0 41.0 34 37.76965411046793 39.0 36.0 40.0 34.0 41.0 35 38.02053095326357 39.0 37.0 40.0 34.0 40.0 36 38.199121731443725 40.0 37.0 40.0 35.0 40.0 37 38.33248738201831 40.0 37.0 40.0 35.0 40.0 38 38.32438906156435 40.0 37.0 40.0 35.0 40.0 39 38.44817360061593 40.0 37.0 40.0 35.0 40.0 40 38.44113034303801 40.0 37.0 40.0 35.0 40.0 41 38.438877641221595 40.0 38.0 40.0 35.0 40.0 42 38.42826998203542 40.0 38.0 40.0 35.0 40.0 43 38.35564173486555 40.0 37.0 40.0 35.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4398464274495453 1101 2 0.13222648688120842 1101 3 -0.024711997019807086 1101 4 0.21023279718521337 1101 5 0.6407923137074718 1101 6 0.4647351283007666 1101 7 -0.058611303096675726 1101 8 0.18373634027314978 1101 9 0.7359701335599738 1101 10 0.5406804251550739 1101 11 0.3894380433888074 1101 12 0.6428286811379706 1101 13 0.5981993465350044 1101 14 0.5326469601432002 1101 15 0.9949207087017626 1101 16 0.5630829709780869 1101 17 1.4545544406894138 1101 18 -0.5290274428217003 1101 19 -0.33114522087692677 1101 20 2.5827628361923303 1101 21 1.0821944793065832 1101 22 0.8376314760568988 1101 23 0.5607408337606401 1101 24 0.04387261913338847 1101 25 0.3558163454428609 1101 26 -0.4383297501758534 1101 27 0.8950086927734731 1101 28 0.7378518182811362 1101 29 0.5613423454292317 1101 30 1.2057661517159275 1101 31 1.4327055831841804 1101 32 0.46041135793254995 1101 33 0.6856345511448012 1101 34 0.6540222816631882 1101 35 0.9644684583588017 1101 36 0.7364250228213933 1101 37 -0.07705153271708554 1101 38 0.5808514926411235 1101 39 0.6068946256462695 1101 40 -0.1969215433709408 1101 41 0.37867719574403225 1101 42 0.009125028675200042 1101 43 0.4920391875375998 1102 1 0.34530097290409145 1102 2 0.3940446686993866 1102 3 0.5325607302529178 1102 4 0.6047782517306644 1102 5 0.9771559500711078 1102 6 0.37473512830076317 1102 7 0.8186614241760495 1102 8 0.5782817948186008 1102 9 0.3777883153781545 1102 10 0.6488622433368931 1102 11 0.6539834979342629 1102 12 0.7801014084106939 1102 13 0.6663811647168245 1102 14 0.35173786923411043 1102 15 0.5594661632472153 1102 16 0.6376284255235412 1102 17 0.6081908043257798 1102 18 -0.38902744282169976 1102 19 -0.21932703905874362 1102 20 -0.5136008001713037 1102 21 -0.9023509752388712 1102 22 -0.900550342124923 1102 23 -1.7856228026029939 1102 24 -0.17430919904843023 1102 25 1.252179981806492 1102 26 0.6243975225514191 1102 27 -0.3522640344992567 1102 28 -0.28487545444613716 1102 29 -0.6650212909344049 1102 30 -0.9342338482840731 1102 31 0.16816012863872487 1102 32 0.5531386306598236 1102 33 -0.2561836306733767 1102 34 0.3303859180268205 1102 35 -0.18734972345937706 1102 36 0.40460684100321487 1102 37 0.6156757400101895 1102 38 0.6399424017320285 1102 39 0.7296218983735443 1102 40 0.541260274810881 1102 41 0.45413174119858013 1102 42 0.6227613923115669 1102 43 0.7165846420830562 1103 1 0.3872617572178143 1103 2 -0.15654356659472768 1103 3 0.38393327927252585 1103 4 -0.0313001796418817 1103 5 0.27715595007110494 1103 6 0.2441468930066506 1103 7 0.16375946339173453 1103 8 0.5010268928578157 1103 9 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1104 2 0.3765446686993883 1104 3 0.6608940635862552 1104 4 0.8106115850640023 1104 5 0.7146559500711049 1104 6 0.9855684616341023 1104 7 0.9394947575093866 1104 8 0.638281794818603 1104 9 0.06362164871148934 1104 10 0.02552891000355828 1104 11 0.30231683126759634 1104 12 0.3492680750773616 1104 13 0.6455478313834888 1104 14 0.3792378692341103 1104 15 0.40779949658054804 1104 16 0.559295092190208 1104 17 0.3181908043257806 1104 18 0.5543058905116354 1104 19 0.1840062942745888 1104 20 1.7797325331620328 1104 21 -0.5181843085722022 1104 22 -0.27221700879158917 1104 23 -1.3672894692696609 1104 24 -0.8368091990484317 1104 25 0.05467998180649403 1104 26 -0.7072691441152443 1104 27 0.5010692988340821 1104 28 -0.034875454446137155 1104 29 0.17497870906559854 1104 30 0.4974328183825918 1104 31 1.0406601286387271 1104 32 1.0248052973264876 1104 33 0.33714970265995703 1104 34 0.873719251360157 1104 35 1.1576502765406218 1104 36 0.32544017433654915 1104 37 0.25817574001018784 1104 38 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-0.671898494402253 2216 15 -0.027806564025510738 2216 16 1.4721738800689934 2216 17 1.1818271679621475 2216 18 0.13006346626920617 2216 19 1.55521841548671 2216 20 0.1054901089196072 2216 21 0.30946720657931337 2216 22 1.7012678396932586 2216 23 0.3107408337606401 2216 24 0.38478171004247486 2216 25 1.3103617999883141 2216 26 0.19803388618778683 2216 27 -0.059536761771980196 2216 28 -0.14851181808250402 2216 29 -0.09774856366167484 2216 30 -1.0896883937386193 2216 31 -0.8627489622703663 2216 32 0.028593176114370067 2216 33 0.3447254602357148 2216 34 -0.732341354700452 2216 35 -0.3764406325502847 2216 36 -0.5590295226331534 2216 37 -0.5770515327170855 2216 38 -0.2827848709952434 2216 39 -0.6431053743537305 2216 40 -1.1969215433709408 2216 41 -0.5076864406196009 2216 42 -0.14996588041570647 2216 43 0.06022100571941991 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 3.0 26 2.0 27 49.0 28 138.0 29 282.0 30 610.0 31 1130.0 32 2243.0 33 4164.0 34 6795.0 35 8001.0 36 5720.0 37 3155.0 38 2777.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.1853488836294163 98.76529128289943 0.8069805241096125 0.2423793093615444 2 98.82517322991816 0.21956713906869313 0.26804300094100203 0.6872166300721435 3 0.1882004049160227 0.3649947246856198 0.13972454304371382 99.30708032735464 4 0.655849895919473 0.7898713963899742 98.20924463201118 0.34503407567937494 5 0.9181898542872623 98.01534118452194 0.42772819299096065 0.6387407681998346 6 98.74818215517979 0.35073711825258774 0.5047192677293336 0.3963614588382902 7 0.8469018221221021 1.220451110667541 96.77492942484815 1.1577176423622002 8 0.9752480894262576 0.5161400707197444 1.3744724535188777 97.13413938633512 9 22.961758918641458 26.033022499786124 23.671828214560698 27.333390367011724 10 25.28516026006616 23.57134709706855 19.90418615261777 31.23930649024752 11 24.722657921003847 25.9917296449451 21.060886924283473 28.224725509767573 12 25.17394775864036 22.778601574084636 19.21694992585833 32.83050074141667 13 23.17146197496364 19.527217770680657 20.174513102740313 37.12680715161539 14 59.004705546841585 22.9459575074861 7.486097247968059 10.563239697704264 15 23.904302945621488 22.7066640052468 42.51333086201489 10.875702187116827 16 14.24416117717512 17.98271879544871 56.40915960874897 11.3639604186272 17 13.693005218283954 19.492999515241383 53.77398842282357 13.040006843651089 18 16.61913995665564 26.020873731036843 12.12216265541234 45.23782365689517 19 42.79194477722631 28.529864795481775 13.283701294996863 15.394489132295053 20 25.19961218204631 32.23166419527775 21.4012775179651 21.167446104710848 21 20.670677806609827 27.65690495879552 24.52308306481508 27.149334169779575 22 22.63537597308164 28.977159314494283 24.089651829250904 24.297812883173172 23 23.305483475434144 29.747070061878013 25.1561207904417 21.791325672246142 24 23.57637799766175 27.080897658901026 26.44215689070119 22.900567452736034 25 23.890045339188458 24.12672160597679 26.93261855199749 25.050614502837266 26 20.408337848242038 26.852775955972515 27.75670820382674 24.98217799195871 27 22.156320396931765 28.803216516011293 27.323276968262565 21.717186118794377 28 22.612563802788788 28.224357694830193 27.3375345746956 21.82554392768542 29 20.693489976902676 28.076078587926656 28.386894408166757 22.843537027003908 30 23.36536542245288 25.98876500613077 29.399184464912032 21.24668510650432 31 22.338817759274573 31.110097236875873 26.795745530240385 19.755339473609173 32 23.64263716208509 27.30409490133455 26.308885593703664 22.744382342876694 33 22.621118366648606 26.80715161538681 28.07037554535345 22.501354472611137 34 22.310302546408508 26.658872508483277 30.71658729932419 20.314237645784026 35 20.8816903818187 30.84490575722148 28.426815706179248 19.846588154780576 36 24.859562576634634 26.978242892583193 28.207248567110554 19.95494596367162 37 23.06156785581886 26.791570422334388 31.223087233011093 18.923774488835658 38 21.996635204881805 26.134192591747695 29.41059055005846 22.45858165331204 39 23.499386922923378 26.191223017479825 29.1197353788246 21.18965468077219 40 22.731420749443906 25.93965664746478 30.665031654594195 20.66389094849712 41 22.104662769143022 28.76657635819193 29.79324112362755 19.335519749037502 42 24.873820183067664 26.44215689070119 29.513245316376285 19.17077760985486 43 22.171842472980295 28.071976502124507 30.290586591382212 19.46559443351299 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 4.0 11 7.0 12 7.0 13 19.0 14 31.0 15 47.0 16 63.0 17 74.5 18 86.0 19 86.0 20 103.0 21 120.0 22 185.0 23 250.0 24 354.0 25 458.0 26 458.0 27 502.5 28 547.0 29 647.5 30 748.0 31 895.5 32 1043.0 33 1043.0 34 1233.0 35 1423.0 36 1624.5 37 1826.0 38 1894.5 39 1963.0 40 1963.0 41 2203.5 42 2444.0 43 2912.0 44 3380.0 45 3307.0 46 3234.0 47 3234.0 48 3416.5 49 3599.0 50 3425.5 51 3252.0 52 3201.5 53 3151.0 54 3151.0 55 2688.5 56 2226.0 57 1958.5 58 1691.0 59 1619.5 60 1548.0 61 1548.0 62 1236.0 63 924.0 64 648.0 65 372.0 66 303.5 67 235.0 68 235.0 69 182.0 70 129.0 71 136.5 72 144.0 73 135.0 74 126.0 75 126.0 76 71.0 77 16.0 78 17.0 79 18.0 80 13.5 81 9.0 82 9.0 83 6.5 84 4.0 85 2.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0028515212866064045 9 0.005703042573212809 10 0.0028515212866064045 11 0.011406085146425618 12 0.0028515212866064045 13 0.0 14 0.011406085146425618 15 0.0 16 0.005703042573212809 17 0.0 18 0.0028515212866064045 19 0.031366734152670446 20 0.0028515212866064045 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0028515212866064045 33 0.0 34 0.0 35 0.0 36 0.0 37 0.005703042573212809 38 0.0 39 0.0 40 0.008554563859819213 41 0.011406085146425618 42 0.0 43 0.005703042573212809 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 35069.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.468875645156686 #Duplication Level Percentage of deduplicated Percentage of total 1 74.69468295024265 39.9384071402093 2 12.25534638152632 13.105591833243036 3 4.794410964748547 7.690552909977473 4 2.4745346914831208 5.292423507941487 5 1.4239240573836063 3.8067809176195504 6 0.911951362593995 2.925660840058171 7 0.6612980641032478 2.475120476774359 8 0.5013065969814944 2.1443440075280162 9 0.3626473254759746 1.7451310274031195 >10 1.8185696762839314 16.436168695999314 >50 0.07999573356087676 2.8686304143260433 >100 0.021332195616233802 1.5711882289201289 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGCTAGGCGCTACGGAGTGAGGGAGAGGCTTGGGACAGGAAGA 169 0.4819070974364824 No Hit AGCTAGTCCCCAGGTTTATGGAACCCGGTGGCTCCCGTCATGT 160 0.4562434058570247 No Hit AGCTAGTCCAAATGGTTCGATCAGGCTACTACAAAGTTCTGGG 115 0.3279249479597365 No Hit AGCTAGTCCCAGGGTTCCATCCAGTACTAAATGCTTACCGTGT 107 0.30511277766688527 No Hit AGCTAGTCCCACAGGATCGGCTCCTAATAGGAGGTGAATTAGA 82 0.23382474550172516 No Hit AGCTAGTCACTGGGTTTTGTAAGACAGAGCTTACTGTGTGACC 80 0.22812170292851236 No Hit AGCTAGTCTGAACGTAAAAGAAGCGGATTTAATTCTGCATTTA 80 0.22812170292851236 No Hit AGCTAGTCGAAACGTTTAGTCCCAGCCTTTAAGCATGATCCTG 78 0.22241866035529959 No Hit AGCAGGTCTCCCCGCGACGGCCTGCGCCAGCGCTGGTTCACGC 75 0.21386409649548033 No Hit AGCTAGTCACCCCGGTTCGATCAGGCTACTACAAAGTTCTGGG 73 0.20816105392226755 No Hit AGCTAGTCCTTCTAATTCGACAACGAAAGTCATATCGAAGAAA 69 0.19675496877584192 No Hit AGCTAGTCCAAGGGGTTCGATCAGGCTACTACAAAGTTCTGGG 69 0.19675496877584192 No Hit AGCTAGTCTCATAGTGACATACTTCCTGTTGCTGTACTTCCAC 69 0.19675496877584192 No Hit AGCTAGTCACAGCGTTTACAGTCCTGACTGAAAGTCACCCTGC 67 0.1910519262026291 No Hit AGCTAGTCACTCTGATTAGATAGGGAAAAGATCGGGATGCTAC 55 0.15683367076335225 No Hit AGCTAGTCACACGGTGTGGGCCAGGCACTATTCCAGCAGATTT 54 0.15398214947674585 No Hit AGCTAGTCAAGCCGGTTGGTTGCCAGAAACTCTTAGGTGAGGG 53 0.15113062819013945 No Hit AGCTAGTCGCTGGGTGAAACCCCGTCTCTACTAAAAATACAAA 51 0.14542758561692662 No Hit AGCTAGTCGTCGCGTTTGGCCGTCGCTGTTGGCCACGTGAAGA 51 0.14542758561692662 No Hit AGCTAGTCGGCACGGGAGGGATGGGGAGTCGCCCTCCCCCGGA 50 0.14257606433032022 No Hit AGCTAGTCTGACTCGTAAGCGAGACTATCTGCGTATTGTAGTA 48 0.13687302175710742 No Hit AGCTAGTCCGGCAGTCCGACGATCGAGTAAGCTAGTCTTTTTT 45 0.12831845789728819 No Hit AGCTAGTCTGATAGTTACATTTGGATGGCTTATGTAACCACAA 45 0.12831845789728819 No Hit AGCTAGTCACACTAGTCCGACGATCTAAACAGCTAGTCTTTTT 44 0.12546693661068178 No Hit AGCTAGTCTTGTTGTTTCTGGAACTATCAAGGAGATCCTGGGT 44 0.12546693661068178 No Hit AGCTAGTCTGTCCGATAAGGAAGACCACTTGCCAAAATCTGAG 43 0.1226154153240754 No Hit AGCTAGTCAGACCGTAGATTTTGTCTTTTCAAGATATAATAAG 41 0.11691237275086258 No Hit AGCTAGTCTGATTGTGTCAAAGTCTGAGTGACTGGGTAGTTGA 40 0.11406085146425618 No Hit AGCTAGTCCCACCGGGTCGGTCATGTCTGTATGTTCTTGTTTG 40 0.11406085146425618 No Hit AGCTAGTCTCGGGGTAACTTTTGTGTATGTACCTAGCTGTACT 40 0.11406085146425618 No Hit AGCTAGTCCGGACGGAGCGCCAGGGGCGGAAATTCGCCTGAAA 39 0.11120933017764979 No Hit AGCTAGTCGGTTGGTTTCTTCCTGTCACCCCCATTCTGGAAGG 38 0.10835780889104336 No Hit AGCTAGTCTTCCTGTTCCAGAATGTCTGGTATCTTCACACAGA 38 0.10835780889104336 No Hit AGCGAGTGCCGCCGATTGCAGCAGCAATCGGCGTATACCCTGT 36 0.10265476631783058 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.05417890444552169 0.0 0.0 16 0.0 0.0 0.05417890444552169 0.0 0.0 17 0.0 0.0 0.05703042573212809 0.0 0.0 18 0.0 0.0 0.05703042573212809 0.0 0.0 19 0.0 0.0 0.08269411731158573 0.0 0.0 20 0.0 0.0 0.08839715988479854 0.0 0.0 21 0.0 0.0 0.09410020245801134 0.0 0.0 22 0.0 0.0 0.20816105392226752 0.0 0.0 23 0.0 0.0 0.29085517123385324 0.0 0.0 24 0.0 0.0 0.2965582138070661 0.0 0.0 25 0.0 0.0 0.30511277766688527 0.0 0.0 26 0.0 0.0 0.3250734266731301 0.0 0.0 27 0.0 0.0 0.39921298012489664 0.0 0.0 28 0.0 0.0 0.4191736291311415 0.0 0.0 29 0.0 0.0 0.4562434058570247 0.0 0.0 30 0.0 0.0 0.50757078901594 0.0 0.0 31 0.0 0.0 0.550343608315036 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTCT 20 0.0017970619 37.08584 13 CGTTTAG 20 0.0017970619 37.08584 13 GAGTGAG 25 1.1929255E-4 37.08584 15 AGGATCG 25 1.1929255E-4 37.08584 13 GTTCCAT 25 1.1929255E-4 37.08584 14 GTTCTAC 25 1.1929255E-4 37.08584 15 TGTTTGA 20 0.0017970619 37.08584 13 GGATCGG 25 1.1929255E-4 37.08584 14 GGTTCGA 25 1.1929255E-4 37.08584 14 GGTTTCT 25 1.1929255E-4 37.08584 13 GGTTTAT 30 7.9561105E-6 37.08584 13 TTGGGAA 20 0.0017970619 37.08584 16 TTTAGAT 20 0.0017970619 37.08584 15 CTTCGGT 20 0.0017970619 37.08584 15 GCTTCGG 25 1.1929255E-4 37.08584 14 TTCGGTT 20 0.0017970619 37.08584 16 ATTTAGC 30 7.9561105E-6 37.08584 14 CGATTGG 20 0.0017970619 37.08584 13 GTTCGAT 35 5.328384E-7 37.08584 15 AGGGGTT 20 0.0018097583 37.03286 11 >>END_MODULE