FastQCFastQC Report
Thu 26 May 2016
SRR2099194_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099194_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426706
Sequences flagged as poor quality0
Sequence length42
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTAGAAATGTTCTACAGTGAGTCGTATTACCGGCCGAC70040.28862169541757426No Hit
ACTGCTAGAAGACGACTCACTATAGTGAGTCGTATTACCGGC68730.28322343126855914No Hit
ACTGCTGCGCCGAGGTGGAACGGCTGCGTGCGTTCGCCCCGC63940.26348474021987006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG200.002106780436.00274311
GCTAGCG105300.035.915674
TGCTAGC375050.035.89393
TGCTAGG573300.035.8320853
TAGTGCG14950.035.798846
TGCTAGA519800.035.7593963
CTAGCGC27600.035.7127235
CTAGCGG32300.035.7044335
CTAGCGT21100.035.697565
TAGCCCG4700.035.6565066
CTAGCGA26850.035.6364945
GCTAGGC169650.035.5787624
CTAGACG44500.035.512755
CTGCTAG1972850.035.5073552
ACTGCTA2035200.035.505741
GCTAGGT145700.035.4913254
GCTAGAT98350.035.467524
GCTAGGG146100.035.3941574
GCTAGAC133050.035.358344
TAGGTCG13950.035.264866
Produced by FastQC (version 0.11.5)