FastQCFastQC Report
Thu 26 May 2016
SRR2099193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144026
Sequences flagged as poor quality0
Sequence length42
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTAGAAGCGTCTCGACGGGCTAGCACACATCTGGTTGA3280.22773665865885329No Hit
ACTACTAGTTACGTCTCGACGGGCTAGCACACATCTGGTTGA2590.17982864205074084No Hit
ACTGCTAGCTGTGTCTCGACGGGCTAGCACACATCTGGTTGA2530.17566272756307888No Hit
ACTGCTAGCGTAGATTGGGCTACATCCATCTGTCATCTGAAG2240.15552747420604615No Hit
ACTGCTAGTCAAGTCTCGACGGGCTAGCACACATCTGGTTGA1880.13053198728007442No Hit
ACTGCTAGACTAGTCTCGACGGGCTAGCACACATCTGGTTGA1860.12914334911752046No Hit
ACTGCTGCGCCGAGGTGGAACGGCTGCGTGCGTTCGCCCCGC1820.1263660727924125No Hit
ACTGCTAGCACTGTTTCGCTCGGGTCTGCGGGTTATAGCTTT1780.12358879646730452No Hit
ACTGCTAGTCGCGTTTCAGTCTCGACGGGCTAGCACACATCT1750.12150583922347354No Hit
ACTGCTAGAGTAGGTTCGGGAAGTGGAGCATCGTTAGCTGCC1470.10206490494771778No Hit
ACTGCTAGAAATGTTCTACAGTGAGTCGTATTACCGGCCGAC1460.10137058586644078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG309.892703E-636.04033712
CGCGACG309.892703E-636.04033712
CGTTTCA650.036.04033712
TGTTTCG356.861992E-736.04033312
GATACGC200.00208948236.04033313
CGTATGG200.00208948236.04033312
CGTATAG200.00208948236.04033312
CGTATAA200.00208948236.04033312
CTTTTCG251.4336235E-436.04033312
CGTCTCG750.036.04033312
GTCTAAG200.00208948236.04033313
TTTTTCG356.861992E-736.04033312
TGTGACG251.4336235E-436.04033312
ATTTTCG200.00208948236.04033312
CGAATTG251.4336235E-436.04033312
GATTCGC551.6370905E-1136.04033313
CGTTTCT356.861992E-736.04033312
CGTTTAG200.00208948236.04033312
TTTTCGC502.3464963E-1036.04033313
ATTTCGC404.7810317E-836.04033313
Produced by FastQC (version 0.11.5)