FastQCFastQC Report
Thu 26 May 2016
SRR2099191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137770
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCTAGCTAGAAATGGGACTGTAGAACTCTGAACCCCTATAGTGA9110.6612470058793641Illumina DpnII expression PCR Primer 2 (95% over 22bp)
ACTGCTAGGCGAAAATGGCGGCCGGCACTCGCCTTCAGCACCGCAGC5090.3694563402772737No Hit
ACTGCTAGGCGTTGGATCAGTCCGACGATCGTCATGTACGTCATTTT4210.305581766712637No Hit
ACTGCTAAAACGCAAAAGTAATTTAAAAGGTGCTGGACGTGTGCGGT3690.26783770051535166No Hit
ACTGCTAGTAATGAGATGAGAGAAAGAAAGAGGAAGGAAGGAAGGAA3460.2511432096973216No Hit
ACTGCTAGGTATTTGGTGGCTGGCCGGAGGTGGCGAGTGCCTATGTG3240.23517456630616243No Hit
ACTGCTAGGTATTTGGTGGGCTGGCCGGAGGTGGCGAGTGCCTATGT3170.23009363431806634No Hit
ACTGCTAGTATTTCCGTGTAAAATATCTAAGCTAATGAATGATGAAT2980.2163025332075198No Hit
ACTGCTAGCTAGAAATGGGGACTGTAGAACTCTGAACCCCTATAGTG2940.2133991435000363Illumina DpnII expression Adapter 1 (95% over 21bp)
ACTGCTAGTAGCTGCGGATAGTGAGTCGTATTACCGGCCGACGATCG2740.19888219496261883No Hit
ACTGCTAGAACTATAGTGGAGTTGGAGTGTTGTGGTTACCTACTGAG2620.1901720258401684No Hit
ACTGCTAGAAGGTAAGCATTTCAGCTGACAACTGAGTGATGAGAAGG2420.17565507730275096No Hit
ACTGCTAGCGGAGTGCTCAGGGGAACCCCAGGGCCAAAAAGGGGGAG2030.14734702765478697No Hit
ACTGCTAGTTGGAACGTTCCTCTGCTAGTGACACCAAGTCAGCAGGC1910.1386368585323365No Hit
ACCGCCAGGGCAACGTGGGGCACAGAAACCACGCGGGTTGGCGAGCA1900.13791101110546564No Hit
ACTGCTAGAGTGTAGTAATTTCACTCCACAGAGGGAGCTGTGGTGTC1900.13791101110546564No Hit
ACTGCTAGGCACGGTTCTCAACTGCTAGTTTTTTTTTTTTTTTTTTT1830.13283007911736952No Hit
ACTGCTAGTCCGAAGGAGTCAACTGCTAGTTTTTTTTTTTTTTTTTT1810.13137838426362777No Hit
ACAGCGAGATTTAGTGTGGCCACGTCGAAGTCGTAGTAGAAGCCGTT1710.12411990999491908No Hit
ACTGCTAGAGCAACCGAGTCAACTGCTAGTTTTTTTTTTTTTTTTTT1700.1233940625680482No Hit
ACTGCTAGGAGCTGTTAGCGGCGTCGCCAGGAGGAGCGCGCGGGCAC1600.11613558829933948No Hit
ACTGCTAGTAGCTGCTCAGCAGCTAGTACGATCGTCGGACTGTAGAA1530.1110546563112434No Hit
ACTGCTAGTGGTGCAATGATCCAAGAGGATGCTGGCCATTAAGAGAT1380.10016694490818029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATT1350.041.0000045
CGTGGGT352.4965448E-741.00000413
TAGACAC553.6379788E-1241.0000046
TAGGTCG352.4965448E-741.0000046
TAGCGAT352.4965448E-741.0000046
TAGTTCC352.4965448E-741.0000046
ATGTACC352.4965448E-741.00000431
AAACGCA700.041.0000048
ATAAGCG352.4965448E-741.0000047
TAGGGCC553.6379788E-1241.0000046
AGCTACG553.6379788E-1241.0000047
GTAACTT352.4965448E-741.00000418
ACGACAT553.6379788E-1241.00000411
AGGTCAT200.001108761941.07
CTTGTAC200.001108761941.041
AACGTCC200.001108761941.015
CCTTATC200.001108761941.010
TAGAGCG1000.041.06
CCCCTGG256.7079396E-541.015
AGTCCGC200.001108761941.07
Produced by FastQC (version 0.11.5)