FastQCFastQC Report
Thu 26 May 2016
SRR2099190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227088
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTGATTTCATGTCATAGCATTGGCAGAGGCCATCGCCATTTTATT13540.5962446276333404No Hit
ACGTGACTAGACCATCAGGGGCGCGGGACATGTGGCGTACGGAAGAC8860.39015711970689776No Hit
GCCTGACTGAGTCTGCACGTGAATGTCAACGGCTACGTGCACTCCAA8740.3848728246318608No Hit
AGGTGAGTTAGCTAAAACCTCGAAATTAGGGTTCTTATCTCAAATAT7330.32278235750017614No Hit
ACGTGACTAAATCGGGACAGTCCGACGATCCAGTATTCTAAGTTCGA7160.3152962728105404No Hit
CCGTGATTGGTACGTCAGTGAAATATGGCGAGGAAAACTGAAAAAGG6560.28887479743535543No Hit
ACGTGACTCCTCCGTATGCGTGCCTCTAATCGCAGGCAGGAGGATTT6540.28799408158951595No Hit
ACGTGACTTCAAATCGACCGCAGTGTCCTGACAAGCCGGAGGAGGAG6280.27654477559360247No Hit
ACGTGACTACAGCTAGAGCGGCGCGTGCGCGGGCGGGGCCCGCGTGC6230.27434298597900375No Hit
ACGTGACTGATTGAGGACAGTCCGACGATCCAGTGGACTGTAGAACT4790.21093144507855988No Hit
ACGTGACTGAAGAAGCATCCGACGATCCAGTCCGGATAGATCGAACT4750.20917001338688085No Hit
ACGTGACAAGTACAAATTAAATTTGTTTCTGTTGCTGAATAAATGAA4750.20917001338688085No Hit
ACTTGACCGAACCCCAGGCTCAGGGCTTTGTCTGCAGGGACTGTGGG4730.20828929754104136No Hit
ACGTGACTGATTAGTTTGCGAAGCTCTCTGCCGCTAGCAGCGCCGCC4720.20784893961812162No Hit
ACGTGACTGGCTAAAATGCGGCGTCGCCAGGAGGAGCGCGCGGGCAC4670.20564715000352288No Hit
GCGTGACGTCGTAGTGCGCGGGCGCCGGAGGTCAGCTTCGGCGCCAG3980.17526245332206017No Hit
ACGTGACTCGTCCCGTAGTAGGTCATAGACACGGCTCAGATCTGGTG3710.16337278940322694No Hit
ACTTGACCGAACCCCAGCTCAGGGCTTTGTCTGCAGGGACTGTGGGG3680.16205171563446769No Hit
ACGTGACTAATGTAAAGATCCAGTACAGCTATGTCGAACTGTAGAAC3510.15456563094483197No Hit
ACGTGGCGCTTCCACTAGCGCTGCCCTTGCTGCCGCCATCGGCCGCA3370.14840062002395546No Hit
GCGTGACGTCGTAGTGCCGGGCGCCGGAGGTCAGCTTCGGCGCCAGA3350.14751990417811597No Hit
ACGTGACTAATACAGGGGTCCGACGATCCAGTGAAGTAGGACTGTAG3230.14223560910307897No Hit
ACGTGACTATGACGTTGGTCCGACGATCCAGTGCAGCTCGTCGGACT3060.13474952441344326No Hit
ACGTGACTTTTAGGGCCCGCGGCGTCGCCAGGAGGAGCGCGCGGGCA3040.13386880856760375No Hit
ACGTGACTAACGCATACCCGACGATCCAGTTGACTCAGATCGAACGT2910.128144155569647No Hit
ACGTGACTCAATGTGGTATAAAAACCAGAAAGAAGCTTTCTTAGAAA2870.126382723877968No Hit
ACGTGACTAGGGGATTTGTCCGACGATCCAGTTATGGACCATCGAAC2860.12594236595504826No Hit
ACGTGACTATGAACTTAGTCCGACGATCCAGTGGAAATACCTCGAAC2850.12550200803212852No Hit
ACGTGACTTAGAGGAAAGAACTCTGAACCCCTATAGTGAGTCGTATT2780.12241950257169028No Hit
ACTTGACCGAACCCCAGTCTCAGGGCTTTGTCTGCAGGGACTGTGGG2780.12241950257169028No Hit
ACGTGACTAGACGTTACGGGGAATCACCTGAACCAGGGAGGCGGAGG2700.11889663918833228No Hit
ACGTGACTGGTTGAATATCCGACGATCCAGTGCGTCGATGTCGAACT2610.11493341788205452No Hit
ACGTGACTGTGTAGAATCAGTCCGACGATCCAGTTCTCCAACATCGA2600.1144930599591348No Hit
ACATGACTCGTAGAACAAACTAAGAAGATCAAAGAGAAATACATTGA2600.1144930599591348No Hit
ACGTGACTCGGCAATTACAGTCCGACGATCCAGTTCAAGTGGATCGT2590.11405270203621505No Hit
ACTTGACCGAACCCCAGGACAAAGCTCAGGGCTTTGTCTGCAGGGAC2440.10744733319241881No Hit
ACGTGACTGAAGCATATTACGACTCACTATAGGGAGTCGTATTACCG2370.10436482773198055No Hit
ACGTGACTAGTATGTTCTGAAGAGGCTTAAGTGGCCTCTTCATGTTG2300.10128232227154231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATTG459.076757E-1041.19570540
CTTCGGA401.5366822E-841.014117
CAGCGTA200.001108404141.014119
ACTATCA401.5366822E-841.014116
CGAACTA200.001108404141.014119
ACTGCCG304.083702E-641.014116
CTGTACG200.001108404141.014117
ACTTGCG401.5366822E-841.014116
TTTCGCA200.001108404141.014118
ACTCTTA304.083702E-641.014116
ACTTCGC200.001108404141.014116
GTCTAAT200.001108404141.014115
TATGCTG304.083702E-641.014118
CTGCGCT401.5366822E-841.014117
TGCGCAA200.001108404141.014118
TCTACGC304.083702E-641.014118
CTAAATG200.001108404141.014117
TGTCTAG200.001108404141.014114
CTTGCGC200.001108404141.014117
CACTAAG200.001108404141.014119
Produced by FastQC (version 0.11.5)