FastQCFastQC Report
Thu 26 May 2016
SRR2099189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50460
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGACTAAATCGGGACAGTCCGACGATCCAGTATTCTAAGTTCGA4300.8521601268331352No Hit
ACTTGATTTCATGTCATAGCATTGGCAGAGGCCATCGCCATTTTATT4070.8065794688862464No Hit
GCCTGACTGAGTCTGCACGTGAATGTCAACGGCTACGTGCACTCCAA3130.6202933016250496No Hit
ACGTGACTAGACCATCAGGGGCGCGGGACATGTGGCGTACGGAAGAC2300.45580657946888625No Hit
ACATGACTTGCCGCCCCGATGTCCACCACAATCGGCATCACGCCATC2240.4439159730479588No Hit
CCGTGATTGGTACGTCAGTGAAATATGGCGAGGAAAACTGAAAAAGG1970.3904082441537852No Hit
ACGTGACTCCTCCGTATGCGTGCCTCTAATCGCAGGCAGGAGGATTT1910.37851763773285774No Hit
AGGTGAGTTAGCTAAAACCTCGAAATTAGGGTTCTTATCTCAAATAT1850.36662703131193025No Hit
ACTTGACCGAACCCCAGGCTCAGGGCTTTGTCTGCAGGGACTGTGGG1700.33690051525961157No Hit
ACGTGACTAGACACGTGCCGGCCGACGATCCAGTAGTCACGTTCGAC1470.2913198573127229No Hit
ACGTGACTGGCTAAAATGCGGCGTCGCCAGGAGGAGCGCGCGGGCAC1460.2893380895759017No Hit
ACGTGACAAGTACAAATTAAATTTGTTTCTGTTGCTGAATAAATGAA1440.2853745541022592No Hit
ACTTGACCGAACCCCAGCTCAGGGCTTTGTCTGCAGGGACTGTGGGG1390.27546571541815296No Hit
ACGTGACTGAAGAAGCATCCGACGATCCAGTCCGGATAGATCGAACT1370.2715021799445105No Hit
ACGTGACTGATTGAGGACAGTCCGACGATCCAGTGGACTGTAGAACT1260.2497027348394768No Hit
GCGTGACGTCGTAGTGCGCGGGCGCCGGAGGTCAGCTTCGGCGCCAG1240.24573919936583433No Hit
ACGTGACTGATTAGTTTGCGAAGCTCTCTGCCGCTAGCAGCGCCGCC1220.24177566389219185No Hit
ACGTGACTACAGCTAGAGCGGCGCGTGCGCGGGCGGGGCCCGCGTGC1150.22790328973444313No Hit
ACGTGGCGCTTCCACTAGCGCTGCCCTTGCTGCCGCCATCGGCCGCA1090.21601268331351564No Hit
ACGTGACTCGCCAAACTGTCGCCTCCCGCTGCGATCTTTGCCTGGGC1060.21006738010305193No Hit
ACGTGACTCGTCCCGTAGTAGGTCATAGACACGGCTCAGATCTGGTG1030.2041220768925882No Hit
ACGTGACTTCAAATCGACCGCAGTGTCCTGACAAGCCGGAGGAGGAG1020.20214030915576697No Hit
GCGTGACGTCGTAGTGCCGGGCGCCGGAGGTCAGCTTCGGCGCCAGA970.19223147047166073No Hit
ACTTGACCGAACCCCAGTCTCAGGGCTTTGTCTGCAGGGACTGTGGG950.18826793499801825No Hit
ACGTGACTATGAACTTAGTCCGACGATCCAGTGGAAATACCTCGAAC940.186286167261197No Hit
ACGTGACTCAATGTGGTATAAAAACCAGAAAGAAGCTTTCTTAGAAA910.18034086405073327No Hit
ACTTGACCGAACCCCAGGACAAAGCTCAGGGCTTTGTCTGCAGGGAC880.17439556084026953No Hit
ACGTGACTATGACGTTGGTCCGACGATCCAGTGCAGCTCGTCGGACT870.1724137931034483No Hit
ACGTGACTGGTTGAATATCCGACGATCCAGTGCGTCGATGTCGAACT800.15854141894569956No Hit
ACGTGACTAACGCATACCCGACGATCCAGTTGACTCAGATCGAACGT750.14863258026159334No Hit
ACGTGACTAGGGGATTTGTCCGACGATCCAGTTATGGACCATCGAAC740.14665081252477208No Hit
ACGTGACTAATACAGGGGTCCGACGATCCAGTGAAGTAGGACTGTAG730.14466904478795084No Hit
ACGTGACTTAGAGGAAAGAACTCTGAACCCCTATAGTGAGTCGTATT730.14466904478795084No Hit
ACGTGACTGTGTAGAATCAGTCCGACGATCCAGTTCTCCAACATCGA720.1426872770511296No Hit
ACGTGACTAAGACATCAAGTCCGACGATCCAGTCTGTGATGGTCGAA670.13277843836702338No Hit
ACGTGACTAGACGTTACGGGGAATCACCTGAACCAGGGAGGCGGAGG670.13277843836702338No Hit
CCGTGATTGGTACGTCAGGAAAACTGAAAAAGATGTTATATTTAGAA660.13079667063020214No Hit
ACGTGACTCGTCCCGTAGGTAGGTCATAGACACGGCTCAGATCTGGT660.13079667063020214No Hit
ACGTGACTAGTATGTTCTGAAGAGGCTTAAGTGGCCTCTTCATGTTG610.12088783194609592No Hit
ACGTGACTAAACATTACTTTAAACTTTACTGGACTTTGTTCTAATTT610.12088783194609592No Hit
ACGTGACTTTTAGGGCCCGCGGCGTCGCCAGGAGGAGCGCGCGGGCA600.11890606420927466No Hit
ACGTGACTCCAGACCTAGGATAAAAGAACATCTCAATGAAGAAAATA600.11890606420927466No Hit
ACGCGATTGAAATAGAACCGACGATCCAGTAGTATGGTGTCGAACTG580.11494252873563218No Hit
ACGTGACTAAAGACGTGACAGTTCCGACGATCCAGTACAACAGCATC570.11296076099881094No Hit
ACGTGACTTATCACAACGTCCGACGATCCAGTAGCGCAACGTCGAAC570.11296076099881094No Hit
ACGTGACTAATACAGGGGGTCCGACGATCCAGTGAAGTAGGACTGTA540.1070154577883472No Hit
ACGTGACCGGTAATTGTAAGAACAAGGTCGGCTCCTGCAGCTGCCGC540.1070154577883472No Hit
ACGTGACAGAAGCCATCCATGAAATTTGTGAAATCTGAATTCAGGGC540.1070154577883472No Hit
ACGTGACCATAGGAACGTTCCCGAGCTCTTCCTGATGTTGAAATCAA530.10503369005152596No Hit
ACGTGACTGATAGTTTGGTCCGACGATCCAGTCCTGGACCATCGAAC530.10503369005152596No Hit
ACGTGACGATTAAGTAACTCACCGCCTGGCCTACCCGCCCGGTCTGG510.10107015457788349No Hit
ACGTGACTCGCCACCGTGCACTGTGGGAAAGAGCTTGTCGCTGCGGT510.10107015457788349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCTAA304.007439E-641.03373335
TACGGAA200.001097624141.0337338
GCGTACG200.001097624141.0337335
GATAGAT200.001097624141.0337335
GTACGGA200.001097624141.0337337
ACTAATA304.034924E-640.9930656
ACTCGTC304.034924E-640.9930656
ACTCGCA304.034924E-640.9930656
CCCAGAG304.034924E-640.99306513
CTTTAAC304.034924E-640.9930657
TGACTTG2200.040.9930654
ATTAGTA1200.040.9930656
ATGACTT600.040.9930653
TGGTACG1900.040.9930658
TGACTCG2650.040.9930654
TGACTCC950.040.9930654
TGACTAG3750.040.9930654
ACGTCGT304.034924E-640.9930656
GTCGTAG304.034924E-640.9930658
TGATTTG600.040.9930654
Produced by FastQC (version 0.11.5)