FastQCFastQC Report
Thu 26 May 2016
SRR2099188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381105
Sequences flagged as poor quality0
Sequence length47
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTGCCTTAGAAGGGCGGCGATGCTGCCAGCGGTCAGCGCGATCA26370.6919352934230725No Hit
GATCTGACCGGCCATAAGCTGGGTGGTGGCCATCTGCGACCCGGCAG19570.5135067763477257No Hit
GATCTGACAACAAACAAGGCAAATGGGATATGATCTAGATTTTGAAA9700.254523031710421No Hit
GACCTGCCACCAGAAGAGGGGAGTGGGGGTGGGTGGGTATGGGGGAC9470.24848794951522543No Hit
GATCTGACAAAAACCATCTTATTCTTCTAACTACCAGTCCAAAACTA9370.24586400073470568No Hit
GATCTGACAAAACGAAATGTGCATTAGGAAACTGACTCACTGCCTAA9310.24428963146639376No Hit
GATCTGACGGTCAAAGAGTTTTTTAAGGGTTTTTTTTCCTGTACTTC7520.19732094829508928No Hit
ACTCTGACATGATAAAGGCAGCTCGGTGTCCGGGTTCATCTGTTCGT7220.18944910195352987No Hit
GATCTGACATCACAAATGAGAGGCTGAGGTAGGTGGAAAACGGGCTA6000.15743692683118826No Hit
GACCTGCCACCAGAAGAGGGGGAGTGGGGGTGGGTGGGTATGGGGGA5990.15717453195313627No Hit
GACCTGCCTTAGAAGGGGCGGCGATGCTGCCAGCGGTCAGCGCGATC5360.1406436546358615No Hit
GATCTGACATCACAAATGGAGAGGCTGAGGTAGGTGGAAAACGGGCT4890.12831109536741842No Hit
GATCTGACAACATAGCAAAGTAGTCTTGGCTGGGCACGGTGGCTCAC4780.12542475170884665No Hit
GATCTGACCTGCGTGTCGCTGGAGTTAAAATGGTTATGATTCAAGAC4470.11729051048923525No Hit
GATCTGACATTATCGAAGGTCACACGGCCCCTAATGGAGGAGCTAGA4400.11545374634287138No Hit
GATCTGACCGGAAAATGTCTTTTTATTTCTCATCTCTAAGAACACAT4260.11178021805014367No Hit
GATCTGACGAGAGACGCCCCACCGCGACGCGGCGCGTGCGCGGGCGG4240.1112554282940397No Hit
GATCTGACAAAATAAACATTACCGGCCGACGATCTCAGAAAAAAAAA4090.10731950512325998No Hit
GATCTGACAACCCATCATGAAGATTCGTGATGTCACCAAGTGCCTGG4010.10522034609884415No Hit
GATCTGACATGAGACAGGTCCAGCACCTCCCCCAGTGGTGGCAGCTG3930.10312118707442831No Hit
ACTCTGACATGATAAAGTCAGCTCGGTGTCCGGGTTCATCTGTTCGT3860.10128442292806446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCCG553.6379788E-1241.0144463
CTGTCGG950.041.0144464
CTGACGG17950.041.0144464
TCTGTCG1350.041.0144463
CTGCCCG256.7163804E-541.0144424
CTGCCAT200.00110930841.0144424
GACGTGT304.098194E-641.0036776
GACCGTA1450.041.0036776
TGACGGC4050.041.0036775
GACCCTT750.041.0036776
CTGCAAC600.041.0036778
CCTATCG650.041.0036778
CGATAGC304.098194E-641.0036778
CGTGGGG352.5103327E-741.0036748
ACACGCG256.726791E-541.0036747
CGTGCGA200.001110742241.0036748
GACACGG900.041.0036746
GACACGC1400.041.0036746
CGGCACA200.001110742241.0036748
TGGCGCT459.549694E-1041.0036745
Produced by FastQC (version 0.11.5)