FastQCFastQC Report
Thu 26 May 2016
SRR2099187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71795
Sequences flagged as poor quality0
Sequence length47
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTGCCTTAGAAGGGCGGCGATGCTGCCAGCGGTCAGCGCGATCA8001.114283724493349No Hit
GATCTGACCGGCCATAAGCTGGGTGGTGGCCATCTGCGACCCGGCAG5410.7535343686886273No Hit
GATCTGACAAAACGAAATGTGCATTAGGAAACTGACTCACTGCCTAA2910.4053207047844558No Hit
GATCTGACAACAAACAAGGCAAATGGGATATGATCTAGATTTTGAAA2910.4053207047844558No Hit
GACCTGCCACCAGAAGAGGGGAGTGGGGGTGGGTGGGTATGGGGGAC2550.35517793718225504No Hit
ACTCTGACATGATAAAGGCAGCTCGGTGTCCGGGTTCATCTGTTCGT2230.3106065882025211No Hit
GATCTGACGGTCAAAGAGTTTTTTAAGGGTTTTTTTTCCTGTACTTC2130.2966780416463542No Hit
GATCTGACATCACAAATGAGAGGCTGAGGTAGGTGGAAAACGGGCTA1780.24792812869977018No Hit
GACCTGCCACCAGAAGAGGGGGAGTGGGGGTGGGTGGGTATGGGGGA1690.23539243679922No Hit
GACCTGCCTTAGAAGGGGCGGCGATGCTGCCAGCGGTCAGCGCGATC1640.22842816352113657No Hit
GATCTGACATTATCGAAGGTCACACGGCCCCTAATGGAGGAGCTAGA1590.22146389024305316No Hit
GATCTGACATCACAAATGGAGAGGCTGAGGTAGGTGGAAAACGGGCT1480.2061424890312696No Hit
GATCTGACCTGCGTGTCGCTGGAGTTAAAATGGTTATGATTCAAGAC1350.18803537850825267No Hit
GATCTGACGAGAGACGCCCCACCGCGACGCGGCGCGTGCGCGGGCGG1290.17967825057455256No Hit
GATCTGACCGGAAAATGTCTTTTTATTTCTCATCTCTAAGAACACAT1250.17410683195208582No Hit
GATCTGACAAAATAAACATTACCGGCCGACGATCTCAGAAAAAAAAA1220.16992826798523575No Hit
ACTCTGACATGATAAAGTCAGCTCGGTGTCCGGGTTCATCTGTTCGT1210.16853541332961905No Hit
GATCTGACAAAAACCATCTTATTCTTCTAACTACCAGTCCAAAACTA1150.16017828539591894No Hit
GATCTGACAACCCATCATGAAGATTCGTGATGTCACCAAGTGCCTGG1140.15878543074030224No Hit
GATCTGACAACATAGCAAAGTAGTCTTGGCTGGGCACGGTGGCTCAC1120.15599972142906887No Hit
GATCTGACATAAACAAAAGTCAGGAGATGGACACCATCCTGGCTAAC1090.1518211574622188No Hit
GATCTGACATCGAAAAATGGAAACCCAAATTTTCGGTGTCCCCGTAA1030.1434640295285187No Hit
GACCTGCCTTAGAAGGGTCGGCGATGCTGCCAGCGGTCAGCGCGATC940.13092833762796852No Hit
GATCTGACGAAAATCAGTGGAGTGACTATATGGATGCCCCCCACCCT940.13092833762796852No Hit
GATCTGACAAATATATAGGGGCCATTGTTAAACTTCAGATGTTCATA890.12396406434988509No Hit
GATCTGACCCCCAAGTTCGTTGACGCCCACCCGGTCTATGACGAGGA830.11560693641618498No Hit
GATCAGAGAGCAAACACGGGGGAACCTGGCAATGCCGGGGTTTACGG820.11421408176056828No Hit
GAGCTGCCTCACTTTGCAAAGCGCGACGCGCTTTACATATTCAGTTT810.11282122710495159No Hit
ACTCTGACATGATAAAGGGGCAGCTCGGTGTCCGGGTTCATCTGTTC810.11282122710495159No Hit
GATCTGACGAAGATATGAGAAAATAGAACATGGATCTGACTTTTTTT790.11003551779371823No Hit
GATCTGCAAAACCGAGGCCGACGATCCAATGTAGAAGGGTCGAACGT750.10446409917125148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATC256.6302746E-541.04745516
ACGCCCC256.6302746E-541.04745514
AGGGCGG900.041.04745514
TGGAGAG256.6302746E-541.04745517
TGTGGAT256.6302746E-541.04745516
GAAATGT352.4518158E-741.0474514
CGGATCT401.5008482E-841.0474517
AGAGTTT200.001098927541.0474515
CAAAGCC200.001098927541.0474514
AAGGGCG850.041.01883313
TAAGATG200.001102710141.0188313
CTAGGCC401.510125E-841.0188312
CCATAAG750.041.0188312
CGTGTCG256.657649E-541.0188312
CTTTGCA304.0414343E-641.0188312
ATCGAAG256.657649E-541.0188312
ATATAGG200.001102710141.0188313
AACACGG256.657649E-541.0188313
CGACATC200.001102710141.0188312
AGACGCC352.4652945E-741.01882612
Produced by FastQC (version 0.11.5)