FastQCFastQC Report
Thu 26 May 2016
SRR2099186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360046
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACTGAAGTGACGGACGACTCACTATAGTGAGTCGTATTACCGGC17290.4802164167911878No Hit
TGCACTGAAGTACTCCAGGACTGTAGAACTCTGAACCCCTATAGTGA14420.4005043799958894Illumina DpnII expression Adapter 1 (95% over 21bp)
TGCACTGAAGTGACGGACGGCCGACGATCAATCAGTGCAAGCTTCCG12300.3416230148369931No Hit
TGCACTGAGCACTGATGAGTTGAACATGGGTCAGTCGGTCCTGAGAG11460.3182926626042228No Hit
TACACAGAACATTGCCCGGCATGCATGCGCTGGAGAGTGTGTGTATG9250.2569116168489582No Hit
TGCACTGAGGTATTTCCGCTATCTTAGGGATTGTTATGTTGACATTA8730.24246901784771946No Hit
TGCACTGAAGTGACGGACCGGCCGACGATCAATCAGTGCAAGCTTCC7840.2177499541725224No Hit
TGCACTGACACATAACCAGGTGGTGATCTCAGGGTCACAGTACAGAG7090.19691928253612037No Hit
TGCACTCTTGGTCGGAATCCGTCTCCGCGCTGAGAATGCCTGATCAG6630.18414313726579382No Hit
TGCACTCTTGGTCGGAAGTCCGTCTCCGCGCTGAGAATGCCTGATCA6350.17636635318820373No Hit
TGCACTGACTCCTGAGACATGTCGTCACCTTTTCGTTGATGTAGTCC6150.17081150741849654No Hit
TGCACTGAAGTGACGGAAGTGAGTCGTATTACCGGCCGACGATCAAT5680.1577576198596846No Hit
TGCACTGAGGAAAAACACAGTCCGACGATCAGTCATAGGACTGAGCT5600.15553568155180172No Hit
TGCACTGACAGGTAGGGCACAGAGCTTGCACTGATTGTCGGAATGTA5480.15220277408997737No Hit
TGCACTCGGGGCTGAGAATCAGTCAAAAGGAAGAGCTTACACTGATC5410.15025857807057988No Hit
TGCACCGGAATAAAAGACCGACGATCAGTCATGTGGAAGAGCTTGCA5200.14442599001238732No Hit
TGCACTGAAGTGACGGAGTCACTGACTGATCGTCGGACTGTAGAACT5070.14081534026207762No Hit
TGCTCTGTAGAAGGAGGAGCCAGGTGTGGTGGCACGTGCCTATAGTC4950.1374824328002533No Hit
TGCACTGAGATGGTTGTTCTGAAGCTTGCACTGATTTTGACTGATCG4430.12303983379901458No Hit
TGCACTGATTCATATGTGTCCGACGATCAGTCATGGAGACAAGCTTG4270.11859595718324882No Hit
TGCACTGAAGTGACGGAATCAGTGCAAGCTTCCGTCACTGACTGATC4240.11776273031779273No Hit
TGCACTGAACATGTGTACATACTAGCTTGCACTGATTGTCGGACTGT4190.11637401887536593No Hit
TGCACTCGCGATCGCGGAGTCGTGCTCCCTAGCTTGCACTGATTTTG4110.11415208056748305No Hit
TGCACTGAAGTACTCCAGGGACTGTAGAACTCTGAACCCCTATAGTG4110.11415208056748305Illumina DpnII expression Adapter 1 (95% over 21bp)
TGCCCTGGCGACATTGCTCCAGACGCGGGTCGTTTTTCTCATAGCAT4080.11331885370202696No Hit
TGCACTCTTGGTCGGAATTCCGTCTCCGCGCTGAGAATGCCTGATCA4020.1116523999711148No Hit
TGCACTGAAGTGACGGAACTCTGAACCCCTATAGTGAGTCGTATTAC3980.11054143081717337No Hit
TGCACTGAAGTGACGGAATCAATCAGTGCAAGCTTCCGTCACTGACT3870.1074862656438344No Hit
TGCACTGAAGTGACGGAGGCCGACGATCAATCAGTGCAAGCTTCCGT3850.10693078106686368No Hit
TGCACTGACAGAATGGTGAAAGAAGCTTGCACTGATTGTCGGACTGT3830.10637529648989295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGC200.001110149841.0075048
CGAACCT256.722343E-541.0075046
CTATAAC200.001110149841.0075045
GGGTACG200.001110149841.0075048
CTGCGAG352.5079862E-741.0075045
ACGATGT200.001110149841.0075048
ATATCGG352.5079862E-741.0075048
GAGCCTA505.820766E-1141.0075047
GCCTATC200.001110149841.0075049
CCGAACC256.722343E-541.0075045
CAGTAGG553.6379788E-1241.0075049
GACGAAT401.5432306E-841.0075047
TTGGGTA200.001110149841.0075046
GCCGTAT200.001110909241.0018118
TCACCGG200.001112429240.99041712
CTAGTAG200.001112429240.99041712
CTTTCGC200.001112429240.99041713
TTACCAG200.001112429240.99041714
CGATATT200.001112429240.99041712
TGCACGC401.5488695E-840.99041714
Produced by FastQC (version 0.11.5)