FastQCFastQC Report
Thu 26 May 2016
SRR2099185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94931
Sequences flagged as poor quality0
Sequence length47
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACTGAAGTGACGGACGACTCACTATAGTGAGTCGTATTACCGGC4820.5077371985968756No Hit
TGCACTGAGCACTGATGAGTTGAACATGGGTCAGTCGGTCCTGAGAG3990.42030527435716464No Hit
TGCACTGAAGTACTCCAGGACTGTAGAACTCTGAACCCCTATAGTGA3890.4097713075812959Illumina DpnII expression Adapter 1 (95% over 21bp)
TGCACTGAAGTGACGGACGGCCGACGATCAATCAGTGCAAGCTTCCG3370.35499468034677817No Hit
TACACAGAACATTGCCCGGCATGCATGCGCTGGAGAGTGTGTGTATG3130.3297131600846931No Hit
TGCACTGAGGTATTTCCGCTATCTTAGGGATTGTTATGTTGACATTA2530.2665093594294804No Hit
TGCACTGACACATAACCAGGTGGTGATCTCAGGGTCACAGTACAGAG2090.2201599056156577No Hit
TGCACTCTTGGTCGGAATCCGTCTCCGCGCTGAGAATGCCTGATCAG2080.2191065089380708No Hit
TGCACTGAAGTGACGGACCGGCCGACGATCAATCAGTGCAAGCTTCC2060.21699971558289707No Hit
TGCACTCTTGGTCGGAAGTCCGTCTCCGCGCTGAGAATGCCTGATCA1910.20119876541909387No Hit
TGCACTGACTCCTGAGACATGTCGTCACCTTTTCGTTGATGTAGTCC1660.17486384847942188No Hit
TGCACTGAGGAAAAACACAGTCCGACGATCAGTCATAGGACTGAGCT1550.16327648502596623No Hit
TGCTCTGTAGAAGGAGGAGCCAGGTGTGGTGGCACGTGCCTATAGTC1480.15590270828285807No Hit
TGCACTGAAGTGACGGAAGTGAGTCGTATTACCGGCCGACGATCAAT1480.15590270828285807No Hit
TGCACTGATGTTACAGAGACAGAAGGCTGTGTACAGCATCTCCAAAG1440.15168912157251055No Hit
TGCACTGACAGGTAGGGCACAGAGCTTGCACTGATTGTCGGAATGTA1440.15168912157251055No Hit
TGCCCTGGCGACATTGCTCCAGACGCGGGTCGTTTTTCTCATAGCAT1390.14642213818457617No Hit
TGCACCGGAATAAAAGACCGACGATCAGTCATGTGGAAGAGCTTGCA1350.14220855147422864No Hit
TGCACTGAGAGGGCGTGCAGTCCGACGATCAGTCGGCACATATAGCT1250.13167458469835985No Hit
TGCACTGAAGTGACGGAGGCCGACGATCAATCAGTGCAAGCTTCCGT1220.12851439466559922No Hit
TGCACTCTTGGTCGGAATTCCGTCTCCGCGCTGAGAATGCCTGATCA1170.12324741127766484No Hit
TGCACTGATTCATATGTGTCCGACGATCAGTCATGGAGACAAGCTTG1170.12324741127766484No Hit
TGCACTCGCGATCGCGGAGTCGTGCTCCCTAGCTTGCACTGATTTTG1130.11903382456731731No Hit
TGCACTGAGTATCTAGTGTGGCAGCAAGGAGGGTCAGTTCATATGCC1120.11798042788973044No Hit
TGCACTGAAGTGACGGAATCAATCAGTGCAAGCTTCCGTCACTGACT1110.11692703121214355No Hit
TGCACTGAAGTGACGGAATCAGTGCAAGCTTCCGTCACTGACTGATC1090.1148202378569698No Hit
TGCACTGACAGAATGGTGAAAGAAGCTTGCACTGATTGTCGGACTGT1050.11060665114662228No Hit
TGCACTGAAGTACTCCAGGGACTGTAGAACTCTGAACCCCTATAGTG1050.11060665114662228Illumina DpnII expression Adapter 1 (95% over 21bp)
CGCACTTACAAGCTTATAACTAGTTACGCGACCCCCGAGCGGTCGGC990.10428627108110101No Hit
TGCACTGAAGTGACGGAACTCTGAACCCCTATAGTGAGTCGTATTAC970.10217947772592725No Hit
TGCACTGAAGTGACGGAGAGTCGTATTACCGGCCGACGATCAATCAG960.10112608104834037No Hit
TGCACTGAAGTGACGGAAGTCGTATTACCGGCCGACGATCAATCAGT960.10112608104834037No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTGC200.001102956641.0305916
TCCGCTA401.5152182E-841.0305915
AGAACCC200.001102956641.0305917
ACGGACC401.5152182E-841.0305913
ACGGAAT505.820766E-1141.0305913
GGACGAC401.5152182E-841.0305915
CGAAGCA200.001102956641.0305917
AAGACCG304.047328E-641.03058614
TGATCCA459.367795E-1041.008966
TCTGCAA256.683477E-541.0089612
TGATATA459.367795E-1041.008966
AGCCGTC256.683477E-541.008968
AGGTAAT256.683477E-541.008968
CGGCGAG256.683477E-541.008969
TACCCTC256.683477E-541.008969
ACTGCAA200.001105824141.008964
GATAAGG256.683477E-541.008967
TGAAGTG6600.041.008966
AAGAGGT200.001105824141.0089610
ACTGACG3600.041.008964
Produced by FastQC (version 0.11.5)