FastQCFastQC Report
Thu 26 May 2016
SRR2099184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868826
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGACACCTCTGGTCCGACGATCGTCGGACTGTAGAACTCTG60130.6920833400473743Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTAGACTGCTCAGAAGAAGTTCGCCAGGGACACTGTCTAGACTCCTT16170.18611321484393883No Hit
CTAGACTGAATAATGCGAACGGGTACCTAGACTGTTTTTTTTTTTTT12440.14318171877913413No Hit
CTATAGTGACACCTCTGGGTACCAGAGGTGTTGACGATCGTCGGACT12360.14226093602171205No Hit
CTAGACTGAGCAGCCAGCGACTCACTATAGTGAGTCGTATTACCGGC10120.11647901881389369No Hit
CCAGACTGCGCGATGCTGTCAAGAGCACGGCGCTGCTAATACCCGGA8750.1007106140930405No Hit
CTATAGTGACACCTCTGGTTGACGATCGTCGGACTGTAGAACTCTGA8690.10002002702497394Illumina DpnII expression Sequencing Primer (100% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAACG6050.040.6601876
AGACTGC165700.040.541283
GACTGCG48650.040.4091074
AGACTGG213550.040.375063
ACTGCGA15500.040.205495
GTGACAC29400.040.092586
AGACTGA233550.040.0861783
TGAACGC3650.039.875767
GACTGGG73700.039.8586124
GACTGGT38750.039.782284
GACTGTC39650.039.7064934
GACTGGC45750.039.69964
GACTGCA49750.039.639254
ACTGGCG10700.039.4663475
GACTGAA74700.039.43484
GACTGCC34200.039.380644
TGACACC30250.039.3726167
ACTGCGC13800.039.3650055
GACTGAC53300.039.3067554
ACTGGAC13850.039.222895