FastQCFastQC Report
Thu 26 May 2016
SRR2099183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135776
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGACACCTCTGGTCCGACGATCGTCGGACTGTAGAACTCTG17021.2535352345038888Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTAGACTGCTCAGAAGAAGTTCGCCAGGGACACTGTCTAGACTCCTT4460.32848220598633043No Hit
CTATAGTGACACCTCTGGGTACCAGAGGTGTTGACGATCGTCGGACT3600.2651425877916569No Hit
CTAGACTGAGCAGCCAGCGACTCACTATAGTGAGTCGTATTACCGGC3430.2526219655903842No Hit
CCAGACTGCGCGATGCTGTCAAGAGCACGGCGCTGCTAATACCCGGA2780.2047489983502239No Hit
CTAGACTGAATAATGCGAACGGGTACCTAGACTGTTTTTTTTTTTTT2620.19296488333726136No Hit
CTATAGTGACACCTCTGGTTGACGATCGTCGGACTGTAGAACTCTGA2370.1745522036295074Illumina DpnII expression Sequencing Primer (100% over 24bp)
CTAGACTGGATTTTTGCTGATTGACCCCGTCCTGGGCACCGTAGGCT2020.14877445203865192No Hit
CTAGACTGATTCAGAAGGTGGCAGCCTCCTGAGAGCCACCAAGATCT2010.14803794485034175No Hit
CTAGACTGGATAAGGGAGTTGCACTTCATGCCGGCTGTTCGGCCGCG1960.14435540890879095No Hit
CTAGCCAGACAACCTACTGAGTGGCACCAATTGCCAATCGGGAACGC1910.14067287296724015No Hit
CTAGACTGCAGGCAGGTGTCCGACGATCGTCATTAATTTACGTACCT1900.13993636577893No Hit
CTATAGTGACACCTCTGCGGCTCACTATAGGTACCAGAGGTGTTGAC1820.13404430827244873No Hit
CTGGACGGACGAAGGATCCACAAGGCTGGTTGCCTGTGCACTTCGTC1680.12373320763610653No Hit
CCAGACTGCGCGATGCTGGTCAAGAGCACGGCGCTGCTAATACCCGG1630.12005067169455574No Hit
CTATAGTGACACCTCTGCTGTAGAACTCTGACCCCTATAGTGAGTCG1590.11710464294131512No Hit
CTAGACAGATAGCGGAAGTATTCAACGCTCGAGGCACCATGGAAAAG1590.11710464294131512No Hit
CTAGACTGGTAGATCGGGCAGGAAGAAGAGAGAGAGAGGAGAGATAA1580.11636813575300496No Hit
CTATAGTGACACCTCTGGGTGTTGACGATCGTCGGACTGTAGAACTC1540.11342210699976432Illumina DpnII expression Sequencing Primer (100% over 21bp)
CTATAGTGACACCTCTGAGTGAGTCGTATTACCGGCTCACTATAGGT1520.111949092623144No Hit
CTAGACTGGAGAGCAATTTTGGCGGGTGTTTATCACCAGTGAGTGCG1500.1104760782465237No Hit
CTAGACTGGGCGACACATTGGCACTAGCTGGTACAGCTGCCTGATGG1450.1067935423049729No Hit
CTAGACTGTAGATGTGGGGTCCAGCTTCGGCGCATCGGCAAAGCCAT1400.10311100636342212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAAG200.001098733541.07530618
TGCCACT200.001104736241.02986519
CATGCAG256.678647E-541.02986514
CGGTTGG304.0607865E-641.02986512
CTCGGAT200.001104736241.02986512
CTCGGAG256.678647E-541.02986512
GGAGTTG256.678647E-541.02986515
ACGTCGC200.001104736241.02986512
CTCTGCG256.678647E-541.02986513
CTAGGAG200.001104736241.02986516
AGAGCGC200.001104736241.02986512
CCACCCG200.001104736241.02986514
AGATCGC200.00111076540.984533
CTCGTTA200.00111076540.984539
GCGACCC304.091791E-640.984539
TGCTTTA304.091791E-640.984537
GGGCTCG650.040.984539
CGCGGAA200.00111076540.9845311
ACACCGT200.00111076540.9845334
TAGCGGA505.820766E-1140.9845310
Produced by FastQC (version 0.11.5)