FastQCFastQC Report
Thu 26 May 2016
SRR2099182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819660
Sequences flagged as poor quality0
Sequence length47
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTAACGTACAACGGCCGACGATCTCAAAACGTAGTCACTGT72540.8850010980162507No Hit
GACTACGTAACGTACAACCGGCCGACGATCTCAAAACGTAGTCACTG52010.6345313910645878No Hit
GACTACGTTCAAAAGCGACGACTCACTATAGGGAGTCGTATTACCGG44260.5399799917038772No Hit
GACTACGTACCTTTCACACGTTTTTTTTTTTTTTTTTTTTTTTTTTT29320.35770929409755264No Hit
GACTACGTATGTAGGATCCTACATTCGAGATCGTCGGACTGTAGAAC21260.2593758387624137No Hit
GACTACGTAACGTACAATACGACTCCACTATAGGGAGTCGTATTACC20710.2526657394529439No Hit
CACGACGTGTCACCCGAAGGAGCTCCTAGCCCAGTTGCAGGGTGCTC17790.21704121220994071No Hit
GACTACGTAACGTACAAGGCCGACGATCTCAAAACGTAGTCACTGTT15460.1886147914989142No Hit
GACTACGTTCAAAAGCGGAGTCGTATTACCGGCCTCAATCAAAAACG13590.16580045384671693No Hit
GACTACGTAAGAGCGGACCATCACAATGCTTCATGATGACAGGACAA13540.1651904448185833No Hit
GACTACGTTCAAAAGCGAGTGAGTCGTATTACCGGCCTCAATCAAAA13280.16201839787228853No Hit
GACTACGTCCACTATGACAGAGCGGTGGCGGCCATGGGGACGGAGGC12620.15396627870092477No Hit
GACTACGTTCAAAAGCGAGTCGTATTACCGGCCTCAATCAAAAACGT12250.14945221189273603No Hit
GACTACGTATGATAATGGGGCAAGCTTCTAGCCTGTATTGCCTCACG12070.147256179391455No Hit
GACTACGTAACGTACAAAGTGAGTCGTATTACCGGCCGACGATCTCA11800.14396213063953348No Hit
GACTACGTAACGTACAAACCGGCCGACGATCTCAAAACGTAGTCACT11500.14030207647073176No Hit
GACTACGTTCAAAAGCGGAACTCTGAACCCCTATAGTGAGTCGTATT10650.12993192299246029No Hit
GACTACGTAACGTACAAGTTGTACGTTTCGAGATCGTCGGACTGTAG10350.1262718688236586No Hit
GACTACGTTCAAAAGCGCGGCCTCAATCAAAAACGTAGTCACTGCGC10220.12468584535051119No Hit
GACTACGTTCAAAAGCGACTCTGAACCCCTATAGTGAGTCGTATTAC10130.12358782909987068No Hit
GACTACGTTCAAAAGCGGTCGTATTACCGGCCTCAATCAAAAACGTA10100.1232218236829905No Hit
GACTACGTTCAAAAGCGACTGCGCTTTTGATCGAGATCGTCGGACTG9610.11724373520728107No Hit
GACTACGTTCAAAAGCGACCCCTATAGTGAGTCGTATTACCGGCCTC9330.11382768464973281No Hit
GACTACGTTCAAAAGCGAGTCACTGCGCTTTTGATCGAGATCGTCGG8970.10943561964717077No Hit
GACTACGTTGACGCTAGGTGACATACTTTCCTTTTGGTAAAGCAAAG8920.10882561061903717No Hit
GACTACGTTCAAAAGCGATTACCGGCCTCAATCAAAAACGTAGTCAC8810.1074835907571432No Hit
GACTACGTTCAAAAGCGCGTATTACCGGCCTCAATCAAAAACGTAGT8770.10699558353463631No Hit
GACTACGTTCAAAAGCGGTCGGACTGTAGAACTCTGAACCCCTATAG8770.10699558353463631Illumina NlaIII expression Adapter 1 (95% over 24bp)
GACTACGTTCAAAAGCGTCAAAAACGTAGTCACTGCGCTTTTGATCG8650.10553156186711564No Hit
GACTACGTTCAAAAGCGCTCTGAACCCCTATAGTGAGTCGTATTACC8540.10418954200522167No Hit
GACTACGTTCAAAAGCGGTAGAACTCTGAACCCCTATAGTGAGTCGT8390.10235951492082082No Hit
GACTACGTTCTGGGTCGGGCAAAAAGTGAGTGGAGATTGGATACAGC8300.10126149867018032No Hit
GACTACGTTCAAAAGCGAAAACGTAGTCACTGCGCTTTTGATCGAGA8300.10126149867018032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGTCG303.953901E-641.22652440
CGGTAAG304.1150997E-640.98755314
GTAACGT55500.040.867287
ACGTAAG24700.040.849035
CTACGTA237100.040.7852173
CTACGTG171650.040.759213
TACGTGT38700.040.7501264
CGTTCAA66000.040.7354356
TACGTAA106900.040.7273254
ACGTTCA73300.040.7073335
CGTAACG57650.040.6949276
AAAAGCG64400.040.693711
TACGTGG50350.040.689244
CTACGTC145900.040.6868253
ACGTAAC63850.040.629665
TAACGTA55400.040.6078958
AAAGCGC15500.040.5884212
CGTACAT4200.040.526816
AACGTAC56150.040.503769
TACGTCA40850.040.462864
Produced by FastQC (version 0.11.5)