FastQCFastQC Report
Thu 26 May 2016
SRR2099181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141425
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACGTAACGTACAACGGCCGACGATCTCAAAACGTAGTCACTGT20331.4375110482587943No Hit
GACTACGTAACGTACAACCGGCCGACGATCTCAAAACGTAGTCACTG14010.9906310765423368No Hit
GACTACGTTCAAAAGCGACGACTCACTATAGGGAGTCGTATTACCGG11900.8414353897825702No Hit
GACTACGTACCTTTCACACGTTTTTTTTTTTTTTTTTTTTTTTTTTT6670.4716280714159448No Hit
GACTACGTAACGTACAATACGACTCCACTATAGGGAGTCGTATTACC5830.4122326321371752No Hit
CACGACGTGTCACCCGAAGGAGCTCCTAGCCCAGTTGCAGGGTGCTC5740.4058688350715927No Hit
GACTACGTAACGTACAAGGCCGACGATCTCAAAACGTAGTCACTGTT4100.28990631076542334No Hit
GACTACGTTCAAAAGCGGAGTCGTATTACCGGCCTCAATCAAAAACG3830.270814919568676No Hit
GACTACGTCCACTATGACAGAGCGGTGGCGGCCATGGGGACGGAGGC3710.26232985681456605No Hit
GACTACGTAAGAGCGGACCATCACAATGCTTCATGATGACAGGACAA3450.24394555418066113No Hit
GACTACGTTCAAAAGCGAGTGAGTCGTATTACCGGCCTCAATCAAAA3410.24111719992929115No Hit
GACTACGTAACGTACAAAGTGAGTCGTATTACCGGCCGACGATCTCA3350.23687466855223616No Hit
GACTACGTTCAAAAGCGAGTCGTATTACCGGCCTCAATCAAAAACGT3200.22626834010959873No Hit
GACTACGTAACGTACAAACCGGCCGACGATCTCAAAACGTAGTCACT3130.22131872016970125No Hit
GACTACGTTCAAAAGCGGTCGTATTACCGGCCTCAATCAAAAACGTA2930.20717694891285135No Hit
GCCTACGTATGTAGGATCCTACATTCGAGATCGTCGGACTGTAGAAC2860.20222732897295387No Hit
GACTACGTTCAAAAGCGCGGCCTCAATCAAAAACGTAGTCACTGCGC2670.18879264627894643No Hit
GAGTTCGTAATCGGTACGGACGTCGTCCGCGAGGAGGTCGCGCGGAT2610.18455011490189147No Hit
GACTACGTTCAAAAGCGTCAAAAACGTAGTCACTGCGCTTTTGATCG2540.17960049496199398No Hit
GACTACGTTCAAAAGCGGAACTCTGAACCCCTATAGTGAGTCGTATT2530.1788934063991515No Hit
GACTACGTTGACGCTAGGTGACATACTTTCCTTTTGGTAAAGCAAAG2480.17535796358493902No Hit
GACTACGTTCAAAAGCGACTCTGAACCCCTATAGTGAGTCGTATTAC2360.16687290083082906No Hit
GACTACGTTCAAAAGCGAAAACGTAGTCACTGCGCTTTTGATCGAGA2280.1612161923280891No Hit
GACGACGAAGGATTATGGCGCCGCCAGCCATGGCCGACCATAGACGC2240.1583878380767191No Hit
GACTACGTTCAAAAGCGATTACCGGCCTCAATCAAAAACGTAGTCAC2240.1583878380767191No Hit
GACTACGTTCAAAAGCGTGAGTCGTATTACCGGCCTCAATCAAAAAC2230.15768074951387662No Hit
GACTACGTTCAAAAGCGACCCCTATAGTGAGTCGTATTACCGGCCTC2200.15555948382534912No Hit
GACTACGTATGTAGGATCCTACATTCGAGATCGTCGGACTGTAGAAC2200.15555948382534912No Hit
GACTACGTTCAAAAGCGGTCGGACTGTAGAACTCTGAACCCCTATAG2190.15485239526250663Illumina NlaIII expression Adapter 1 (95% over 24bp)
GACTACGTTCAAAAGCGCGTATTACCGGCCTCAATCAAAAACGTAGT2180.15414530669966411No Hit
GACTACGTTCAAAAGCGGTAGAACTCTGAACCCCTATAGTGAGTCGT2160.15273112957397914No Hit
GACTACGTTCTGGGTCGGGCAAAAAGTGAGTGGAGATTGGATACAGC2150.15202404101113665No Hit
GACTACGTTCAAAAGCGATCAAAAACGTAGTCACTGCGCTTTTGATC2140.15131695244829413No Hit
GACTACGTAGCCCTTGTACTGGAGCCAGCCGGGCACGGTGGCTCACA2140.15131695244829413No Hit
GACTACGTAACGTACAAGCCGACGATCTCAAAACGTAGTCACTGTTG2120.14990277532260915No Hit
GACTACGTAACGTACAAACTCTGAACCCCTATAGTGAGTCGTATTAC2090.14778150963408168No Hit
GACTACGTTCAAAAGCGCTCTGAACCCCTATAGTGAGTCGTATTACC2070.14636733250839667No Hit
GACTACGTAACGTACAAGTAGAACTCTGAACCCCTATAGTGAGTCGT2060.14566024394555419No Hit
GACTACGTTCAAAAGCGAGAACTCTGAACCCCTATAGTGAGTCGTAT2060.14566024394555419No Hit
GACTACGTTCAAAAGCGGGACTGTAGAACTCTGAACCCCTATAGTGA2030.1435389782570267Illumina DpnII expression Sequencing Primer (95% over 21bp)
GACTACGTAACGTACAAGAACCCCTATAGTGAGTCGTATTACCGGCC2000.14141771256849922No Hit
GACTACGTTCAAAAGCGACCGGCCTCAATCAAAAACGTAGTCACTGC1990.1407106240056567No Hit
GACTACGTAACGTACAAGAGTCGTATTACCGGCCGACGATCTCAAAA1980.14000353544281421No Hit
GACTACGTTCAAAAGCGAACCCCTATAGTGAGTCGTATTACCGGCCT1980.14000353544281421No Hit
GACTACGTTCAAAAGCGTCGTATTACCGGCCTCAATCAAAAACGTAG1970.13929644687997172No Hit
GACTACGTTCAAAAGCGGAACCCCTATAGTGAGTCGTATTACCGGCC1960.13858935831712924No Hit
GACTACGTCAGGAATAGTAGGAAGGTTTAGGACATGGCTCAAGGCGA1940.13717518119144423No Hit
GACTACGTTCAAAAGCGGTATTACCGGCCTCAATCAAAAACGTAGTC1930.13646809262860174No Hit
GACTACGTAACGTACAAGAACTCTGAACCCCTATAGTGAGTCGTATT1920.13576100406575922No Hit
GACTACGTTCAAAAGCGTACCGGCCTCAATCAAAAACGTAGTCACTG1830.12939720700017676No Hit
GACTACGTAACGTACAAAGTCGTATTACCGGCCGACGATCTCAAAAC1820.12869011843733427No Hit
GACTACGTATGTTTTTGTTTTTGTGGGAGCGAAGCGGTGGCTGGGCT1760.12444758706027931No Hit
GACTACGTAACGTACAAAGAACTCTGAACCCCTATAGTGAGTCGTAT1730.12232632137175181No Hit
GACTACGTATCCAGGTTAGTCCGACGATCTCGAAAGGTCTGTAGAAC1710.12091214424606682No Hit
GACTACGTCAAGAGATGGGCATTGAGGCAGCCAGCGCAGGGGCTTCT1700.12020505568322432No Hit
GACTACGTTCAAAAGCGCTATAGTGAGTCGTATTACCGGCCTCAATC1690.11949796712038183No Hit
GACTACGTCCTACCTACGCGCACGGAGTTTCTGCAGCGGGAATCCGA1690.11949796712038183No Hit
GACTACGTAACGTACAATGAGTCGTATTACCGGCCGACGATCTCAAA1690.11949796712038183No Hit
GACTACGTAAGGACATGAGGAGGGTGGATGTCTGCGAGTGCTGTCTT1670.11808378999469685No Hit
GACTACGTTCAAAAGCGTGTAGAACTCTGAACCCCTATAGTGAGTCG1660.11737670143185433No Hit
GACTACGTTCAAAAGCGAACTCTGAACCCCTATAGTGAGTCGTATTA1650.11666961286901185No Hit
GACTACGTTCAAAAGCGAACGTAGTCACTGCGCTTTTGATCGAGATC1610.11384125861764186No Hit
GTATACGTGAAGGACACGCGGTTCGCTCATCGCCATCGTCATGTCGC1590.11242708149195686No Hit
GACTACGTTCAAAAGCGCGTCGGACTGTAGAACTCTGAACCCCTATA1580.11171999292911437Illumina DpnII expression Sequencing Primer (95% over 24bp)
GACTACGTAACGTACAAAAACGTAGTCACTGTTGTACGTTTCGAGAT1540.1088916386777444No Hit
GACTACGTAACGTACAAGGACTGTAGAACTCTGAACCCCTATAGTGA1540.1088916386777444Illumina DpnII expression Adapter 1 (95% over 21bp)
GACTACGTCGACGTCCGCCGGCCGACGATCTCGACGTAGTCACTGCG1520.10747746155205941No Hit
GACTACGTTCAAAAGCGTCAATCAAAAACGTAGTCACTGCGCTTTTG1510.1067703729892169No Hit
GACTACATTTCCGTTTTGCTGAATCTATTTCAGTTCATGTGCTGAAT1500.10606328442637442No Hit
GACTACGTAACGTACAAACCCCTATAGTGAGTCGTATTACCGGCCGA1480.10464910730068941No Hit
GACTACGTATGATAATGGGGCAAGCTTCTAGCCTGTATTGCCTCACG1430.10111366448647693No Hit
GACTACGTTCAAAAGCGCTGTAGAACTCTGAACCCCTATAGTGAGTC1420.10040657592363443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT200.001105991741.02158717
GAGGTCT200.001105991741.02158718
ATACGTT200.001105991741.02158717
GATTCCA200.001105991741.02158718
CGGACGT401.5272235E-841.02158717
GCTATAG304.0676423E-641.02158417
GCGAGAA200.001107919541.00707615
CTTCGTT200.001107919541.0070763
CTTCGTA401.5319529E-841.0070763
GTTGACG600.041.0070767
CGTGGGC304.077556E-641.0070766
CTATGGA200.001107919541.0070763
AGCGTCG352.49378E-741.00707614
CGTGGCT200.001107919541.0070766
AGCGTAG553.6379788E-1241.00707614
CGGAACT304.077556E-641.00707616
GTCGCTC200.001107919541.0070767
GTCGCTA600.041.0070767
GTACCTT950.041.0070767
CGGCGCA200.001107919541.00707612
Produced by FastQC (version 0.11.5)