##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099181_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141425
Sequences flagged as poor quality	0
Sequence length	47
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.61304578398445	37.0	33.0	38.0	27.0	40.0
2	31.634802899063107	34.0	27.0	37.0	18.0	39.0
3	35.805175888280004	38.0	34.0	39.0	30.0	40.0
4	36.30688350715927	39.0	37.0	40.0	30.0	40.0
5	35.04786989570444	38.0	34.0	39.0	27.0	40.0
6	36.34563903128867	39.0	37.0	39.0	30.0	40.0
7	36.70603500088386	39.0	37.0	40.0	30.0	40.0
8	34.00820222732897	37.0	32.0	39.0	25.0	39.0
9	35.35034117023157	37.0	35.0	37.0	32.0	37.0
10	35.240473749337106	37.0	35.0	37.0	32.0	37.0
11	35.08574863001591	37.0	35.0	37.0	32.0	37.0
12	35.18363797065582	37.0	35.0	37.0	32.0	37.0
13	36.78907548170408	39.0	37.0	39.0	32.0	39.0
14	36.723903128866894	39.0	37.0	39.0	32.0	39.0
15	36.775789287608276	39.0	37.0	39.0	32.0	39.0
16	36.601583878380765	39.0	37.0	39.0	32.0	39.0
17	36.408258794414	39.0	37.0	39.0	31.0	39.0
18	31.6896022626834	31.0	31.0	34.0	30.0	34.0
19	31.624620823758175	31.0	31.0	34.0	30.0	34.0
20	31.3938059041895	31.0	31.0	34.0	27.0	34.0
21	34.29860350008838	35.0	35.0	37.0	28.0	37.0
22	34.86116316068588	35.0	35.0	37.0	32.0	37.0
23	34.56431324023334	36.0	35.0	37.0	30.0	37.0
24	34.24596782747039	36.0	35.0	37.0	28.0	37.0
25	34.42437334276118	36.0	35.0	37.0	30.0	37.0
26	36.13539331801308	39.0	35.0	39.0	30.0	39.0
27	36.32774261976313	39.0	35.0	39.0	31.0	39.0
28	36.77647516351423	39.0	35.0	39.0	32.0	39.0
29	36.99874845324377	39.0	37.0	39.0	33.0	39.0
30	37.16305462259148	39.0	37.0	39.0	33.0	39.0
31	38.64740321725296	40.0	38.0	41.0	35.0	41.0
32	38.90719462612692	40.0	38.0	41.0	36.0	41.0
33	39.09707618879265	40.0	38.0	41.0	36.0	41.0
34	39.16359200989924	40.0	38.0	41.0	36.0	41.0
35	39.18129043662719	40.0	38.0	41.0	36.0	41.0
36	39.17795297861057	40.0	39.0	41.0	36.0	41.0
37	39.117334276118086	40.0	39.0	41.0	36.0	41.0
38	38.99791408873961	40.0	38.0	41.0	36.0	41.0
39	38.903729892169	40.0	38.0	41.0	35.0	41.0
40	38.80202227328973	40.0	38.0	41.0	35.0	41.0
41	38.667251193211946	40.0	38.0	41.0	35.0	41.0
42	38.43718578751989	40.0	38.0	41.0	35.0	41.0
43	38.308403747569386	40.0	38.0	41.0	34.0	41.0
44	38.26141064168287	40.0	38.0	41.0	34.0	41.0
45	38.19643627364327	40.0	38.0	41.0	34.0	41.0
46	38.18459254021566	40.0	38.0	41.0	34.0	41.0
47	38.1201979847976	40.0	38.0	41.0	34.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	-0.942797098372381
1101	2	-0.3870725959426089
1101	3	-0.34412462493776275
1101	4	-0.11077172412108638
1101	5	0.49533732713789647
1101	6	0.7024526321531539
1101	7	-0.08655121092143503
1101	8	0.4792167537211469
1101	9	0.4182922925685091
1101	10	0.4112102880917803
1101	11	0.6857890754359772
1101	12	0.4503191089548295
1101	13	0.584319225532127
1101	14	0.6120756384036952
1101	15	0.9110608521537884
1101	16	0.8138116458184186
1101	17	0.9334400522623127
1101	18	0.035790865096085867
1101	19	0.2227098113311463
1101	20	-0.24844010388610016
1101	21	-0.29924006556758087
1101	22	-0.3727256129057466
1101	23	-0.7870001006297471
1101	24	-0.6613363452429724
1101	25	-0.9642699958624945
1101	26	-0.7652974900735288
1101	27	-0.6143526199717257
1101	28	-0.36343260754792794
1101	29	-0.4972265992106628
1101	30	-0.2506805299839101
1101	31	-0.27649790817319086
1101	32	-0.30648523266670935
1101	33	-0.008314428597806511
1101	34	-0.08880311791032369
1101	35	-0.06983126905753778
1101	36	0.0757191730077551
1101	37	0.09359202833789482
1101	38	0.045892129662462366
1101	39	0.19806436121156423
1101	40	0.09802470041309874
1101	41	0.02301954002829376
1101	42	-0.08244337213634623
1101	43	-0.16238350591182638
1101	44	-0.02694830346786148
1101	45	-0.0601049153379023
1101	46	-0.17674737030187515
1101	47	0.17957431411374358
1102	1	0.09877146540126347
1102	2	0.6779188792542712
1102	3	0.7601215389008331
1102	4	1.676879489648499
1102	5	1.735371426350362
1102	6	1.416587567608218
1102	7	1.0233781549955907
1102	8	2.0256160392614504
1102	9	0.7111395768097992
1102	10	0.7742045237011013
1102	11	0.5711913649545295
1102	12	0.6835739599737423
1102	13	0.6677405131833396
1102	14	0.6922493820100968
1102	15	0.8408733064852001
1102	16	0.8190889048912453
1102	17	0.8170155982556508
1102	18	-0.08534252777555551
1102	19	-0.289021941530752
1102	20	-0.40493113254567703
1102	21	-0.553482169976121
1102	22	-0.6859512360282594
1102	23	-0.805105158679595
1102	24	-1.067807076752267
1102	25	-0.76093314435645
1102	26	-0.35257523627796417
1102	27	-0.5284144856857793
1102	28	-0.24871311416480069
1102	29	-0.1421888465111394
1102	30	-0.2732509610953855
1102	31	-0.24613337135416202
1102	32	-0.1975707242636915
1102	33	-0.12514482561006446
1102	34	0.0736958672321677
1102	35	0.20044558407228408
1102	36	0.12625907720520502
1102	37	0.15233198124850134
1102	38	0.001157210445121848
1102	39	0.16083938759786065
1102	40	0.08970286879826261
1102	41	0.33652932325472307
1102	42	0.20005236546209204
1102	43	0.016962113963138847
1102	44	0.2318403626034211
1102	45	0.01162521375197656
1102	46	0.19351324510772372
1102	47	0.22650132556133684
1103	1	-0.14961453527831736
1103	2	0.13820600123864324
1103	3	0.16238963268268947
1103	4	0.4859178858428592
1103	5	0.3108896050795522
1103	6	0.5848682335953086
1103	7	0.3252809752470327
1103	8	-0.6184971105856434
1103	9	0.2854260192155067
1103	10	0.18628403440446561
1103	11	0.3641322416110029
1103	12	0.5570873816523019
1103	13	0.4407187666661798
1103	14	-0.026362578574342876
1103	15	0.14774554569689258
1103	16	-0.09388255280497759
1103	17	0.036561979499282415
1103	18	-0.14017603100355203
1103	19	-0.027044367626569255
1103	20	-0.7248003136533896
1103	21	-0.11651309080521344
1103	22	-0.06880886429873101
1103	23	-0.7427113428454177
1103	24	-0.7840829856887268
1103	25	-0.8107547549578769
1103	26	-0.8916085356323578
1103	27	-0.692277890374875
1103	28	-0.5960202837196746
1103	29	-0.9868201750874235
1103	30	-0.6726733192333398
1103	31	-0.36782042538066406
1103	32	-0.06144262368604814
1103	33	-0.06542685075447707
1103	34	-0.04930787045046969
1103	35	-0.08302366499329139
1103	36	0.03184011696055933
1103	37	0.11329868189410774
1103	38	-0.12859984018349024
1103	39	-0.06996251522238595
1103	40	0.25072053784344916
1103	41	-0.006233164003177194
1103	42	0.04362185238020544
1103	43	-0.21158868454178759
1103	44	-0.10671723168811553
1103	45	-0.028639821925899867
1103	46	-0.10431210317634054
1103	47	0.26655399290758197
1104	1	-0.47947059220860666
1104	2	-0.34968418152053715
1104	3	-0.5057932882294907
1104	4	-0.3128044172462552
1104	5	-0.012294933170487354
1104	6	0.15712280257476863
1104	7	-0.16727927058831682
1104	8	0.2645087118306648
1104	9	-0.20262589732985958
1104	10	0.34603658171160134
1104	11	0.16501773409038378
1104	12	0.014430095545236554
1104	13	-0.1676226533564602
1104	14	0.13203544827346292
1104	15	0.08950116594596125
1104	16	0.07066135791797024
1104	17	0.07325533224067016
1104	18	-0.14367352412986634
1104	19	-0.15028551270177815
1104	20	-0.17128664714845598
1104	21	-0.12387197807676387
1104	22	0.031668250370522344
1104	23	-0.06328792379317605
1104	24	-0.31481240506442987
1104	25	-0.1574917758215335
1104	26	-0.5692933164830833
1104	27	-0.3792017137615744
1104	28	-0.37272657516395924
1104	29	-0.3019180240280619
1104	30	-0.2972083345980465
1104	31	-0.17161712601142654
1104	32	-0.24875056481494795
1104	33	-0.026651175515922887
1104	34	-0.08076509186402347
1104	35	0.020364657827343535
1104	36	-0.04548884956054877
1104	37	0.14303748182940978
1104	38	0.06732042505991842
1104	39	-0.03919266240013286
1104	40	-0.13644992956719193
1104	41	0.005532968941999172
1104	42	0.09673118441966722
1104	43	0.32490250096686424
1104	44	-0.1297036460500749
1104	45	-0.1266504963557935
1104	46	-0.014711585628226942
1104	47	0.15845517356422079
1105	1	0.9244595387957588
1105	2	0.4990197966854666
1105	3	0.3748004379834029
1105	4	0.6585308657952922
1105	5	0.5243622520384363
1105	6	0.5037435309109668
1105	7	0.2894332008671512
1105	8	0.8283683328394389
1105	9	0.49095609057126666
1105	10	0.5219109457194548
1105	11	0.6583473282572783
1105	12	0.4266932260287888
1105	13	0.5943460177704978
1105	14	0.6830750700834898
1105	15	0.6252769762099746
1105	16	0.6493641342490477
1105	17	0.5830706441272113
1105	18	-0.31928748190399503
1105	19	-0.3670035929068973
1105	20	-0.3819953527291631
1105	21	-0.6452252892421768
1105	22	-0.18595123602825936
1105	23	-0.4840042412484067
1105	24	-0.7191832235412576
1105	25	-0.27469461224635694
1105	26	-0.23789633719539438
1105	27	-0.5788732012821072
1105	28	-0.25330027012810064
1105	29	0.013774456241151256
1105	30	-0.40169132806786223
1105	31	-0.23237190346425507
1105	32	-0.04619457747470079
1105	33	-0.037988862307315685
1105	34	-0.14648761900636487
1105	35	0.07200521709980734
1105	36	0.02992880197584924
1105	37	0.07893748583565952
1105	38	-0.1364574684539619
1105	39	-0.4171422637782811
1105	40	-0.2864439201925677
1105	41	-0.5166816859195862
1105	42	-0.3458191941709359
1105	43	-0.48303788603686115
1105	44	-0.47916881170850445
1105	45	-0.15809955689022814
1105	46	-0.21474363562622045
1105	47	-0.8147830781083911
1106	1	0.7971010482297203
1106	2	0.9669097049423456
1106	3	0.4142240142340512
1106	4	0.5507327006576759
1106	5	0.7963892556735459
1106	6	0.5252945089272387
1106	7	0.7197603602924616
1106	8	0.6275720718042948
1106	9	0.4948075705781889
1106	10	0.2271645375491218
1106	11	0.1930106483195999
1106	12	0.23563385265160264
1106	13	0.3936363068478741
1106	14	0.4815864081482246
1106	15	0.36848279237753445
1106	16	0.4011384115556069
1106	17	0.6437422730003277
1106	18	-0.20106002820830327
1106	19	-0.1014678477104276
1106	20	-0.21283315781830936
1106	21	0.07932897767836522
1106	22	-0.832610401685173
1106	23	-0.24175047631850788
1106	24	-0.02604664746370844
1106	25	-0.653784105062698
1106	26	-0.7737713588329171
1106	27	-0.689657345301498
1106	28	-0.31501050035139855
1106	29	-0.2427600772537417
1106	30	0.034175038558416304
1106	31	0.05644582199878556
1106	32	-0.03148105834712567
1106	33	0.019653512627769487
1106	34	0.0797976501644797
1106	35	-0.1943094791093003
1106	36	-0.27554982014635954
1106	37	-0.10848678281588775
1106	38	-0.08885490657063144
1106	39	0.040275080864262236
1106	40	-0.06435633147869879
1106	41	-0.06965035477885806
1106	42	0.11341570283443048
1106	43	0.24290118268393002
1106	44	0.08851337627834255
1106	45	0.025343406585626838
1106	46	0.11336784091869845
1106	47	-0.06616787990863315
1107	1	0.575144470302611
1107	2	1.5080055953533034
1107	3	0.8813774561225713
1107	4	0.8600934312512862
1107	5	0.841965201223644
1107	6	0.5048327186282862
1107	7	0.6735813303967078
1107	8	0.7656353095056829
1107	9	0.33660562347730405
1107	10	0.04358997047752666
1107	11	0.4358597910782791
1107	12	0.28704679311856296
1107	13	0.2774133379497954
1107	14	0.44389363654488534
1107	15	0.2854294624903986
1107	16	0.4839332767231781
1107	17	0.1813488896971478
1107	18	0.19055709938711018
1107	19	-0.14880996884422615
1107	20	0.28934225357136967
1107	21	-0.06806052865401568
1107	22	0.011987685675897808
1107	23	-0.31532676648823355
1107	24	-0.09128745042743702
1107	25	-0.06969691629729624
1107	26	-0.031956074952503855
1107	27	0.28527157690061955
1107	28	-0.5112282999550857
1107	29	-0.2279414333319707
1107	30	-0.14020249224196135
1107	31	-0.014890440601369903
1107	32	0.07319715307701813
1107	33	-0.05642126983109108
1107	34	0.13663834974232003
1107	35	0.10736192607723183
1107	36	0.1340090667322471
1107	37	0.009480822939259781
1107	38	-0.044442052258581555
1107	39	0.09657731227634514
1107	40	0.039977260319354
1107	41	0.3959738375188948
1107	42	0.2840454952011058
1107	43	0.19359903742675755
1107	44	0.25268155065587194
1107	45	0.26664176102691073
1107	46	0.39289743512908615
1107	47	0.4350598275093063
1108	1	0.11676723110345222
1108	2	0.3809368542636129
1108	3	0.034624424035129664
1108	4	0.38896799477532085
1108	5	-0.06538825649242597
1108	6	-0.040529788861540794
1108	7	0.4947966447627081
1108	8	0.6690217431096812
1108	9	-0.031253216581376364
1108	10	0.036585693529659125
1108	11	0.11457216116856728
1108	12	-0.038228522487131045
1108	13	0.04581764382388087
1108	14	0.189218953183655
1108	15	0.21224613225630407
1108	16	0.048965612102009004
1108	17	-0.06820172085265597
1108	18	0.0018341918890243392
1108	19	0.04635935283597448
1108	20	-0.0966161339986229
1108	21	-0.13302054848913514
1108	22	0.09479931401532582
1108	23	0.09437178100241539
1108	24	0.1610318123672485
1108	25	0.21708586847098132
1108	26	0.2657360261239674
1108	27	0.26159340173171586
1108	28	0.02064219323691674
1108	29	-0.29304608425450596
1108	30	-0.113011847807158
1108	31	-0.10166909305286254
1108	32	-0.16637391244086785
1108	33	-0.16970873444812895
1108	34	-0.25049774811093783
1108	35	-0.36587099195826767
1108	36	-2.2628137479330235E-5
1108	37	0.044398899759030996
1108	38	0.2411630222264307
1108	39	0.1010195528185065
1108	40	0.005224476170916148
1108	41	0.4544254584201539
1108	42	0.39997764255952006
1108	43	0.23438500407895901
1108	44	-0.04104352630852759
1108	45	0.2629639990896848
1108	46	0.25684090503548873
1108	47	0.19493089922566753
1109	1	0.21515721321436132
1109	2	-0.32843913226695776
1109	3	-0.238320961632553
1109	4	0.1681745611227896
1109	5	3.3835615621313764E-4
1109	6	-0.1181894752763597
1109	7	-0.13561480425339312
1109	8	0.20611529251513616
1109	9	-0.6606334123090392
1109	10	-0.248987283424988
1109	11	-0.03386517440966941
1109	12	-0.3884337208455406
1109	13	-0.5162364443587961
1109	14	-0.31161236324273034
1109	15	-0.10830527256322853
1109	16	-0.11022622205562982
1109	17	-0.41259822721906403
1109	18	-0.5284404582214677
1109	19	-0.39190224876352175
1109	20	-0.18231538686613646
1109	21	-0.3763752785743719
1109	22	-0.19546691770096913
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2214	1	0.037792872129095656
2214	2	-0.8442014061687644
2214	3	-0.8803881450950897
2214	4	-0.5681561882312138
2214	5	-0.3961474319574876
2214	6	-0.582168803441732
2214	7	-0.6621264321603704
2214	8	-0.7101637772523048
2214	9	-0.05846286967338443
2214	10	-0.2214937433733084
2214	11	-0.30642331394455624
2214	12	0.10634664091156054
2214	13	-0.2716886407412247
2214	14	-0.27988109709286135
2214	15	0.07867147162281896
2214	16	0.0822285583061273
2214	17	-0.21177135383405243
2214	18	0.3766758208482983
2214	19	0.2974000768178726
2214	20	0.6187182048651287
2214	21	0.8338572795651586
2214	22	0.6871170616271911
2214	23	0.46969606456199386
2214	24	1.1144355328297948
2214	25	0.7398008005976777
2214	26	1.1830210939972687
2214	27	0.3710554429584647
2214	28	0.5804611977618066
2214	29	0.22466130639406146
2214	30	0.3088081622481411
2214	31	0.47606846350821996
2214	32	0.007261080017656241
2214	33	0.04086944554181571
2214	34	0.04032175917915737
2214	35	-0.01154218351181413
2214	36	0.09906233918277962
2214	37	0.12126164485706425
2214	38	0.22380756722391482
2214	39	0.13188933662946312
2214	40	0.12868350080641733
2214	41	0.42154461377460706
2214	42	0.17677000052835012
2214	43	0.4337816858285848
2214	44	0.2893938794229953
2214	45	0.05136017807409843
2214	46	0.24531752645328453
2214	47	0.4519243632779535
2215	1	0.31386046045474814
2215	2	0.1581539445736837
2215	3	-0.11340401811096257
2215	4	-0.7430461011856409
2215	5	-0.9205918764019287
2215	6	-0.6909040824995927
2215	7	-0.3609282754783436
2215	8	0.1405274669873222
2215	9	-0.2749298435597112
2215	10	-0.3870646599119709
2215	11	-0.33370441993533717
2215	12	-0.06831797762402658
2215	13	-0.21022422701874888
2215	14	0.09957614817083282
2215	15	-0.36814880487487756
2215	16	-0.5984268436395936
2215	17	-0.7626571705263174
2215	18	-0.20710298099501756
2215	19	0.06716966207132913
2215	20	0.3644427312348171
2215	21	0.4911844685052529
2215	22	0.7709675480583229
2215	23	1.1476069708599965
2215	24	0.8360740376941038
2215	25	0.9101233812428404
2215	26	0.8768459787898948
2215	27	0.8393401331786379
2215	28	0.504700829098212
2215	29	0.09642781945089496
2215	30	-0.025037203855291068
2215	31	0.06427122848518252
2215	32	-0.1805115774324193
2215	33	-0.3926584602441494
2215	34	-0.34927373493246705
2215	35	0.00867287025162966
2215	36	-0.43916602743268385
2215	37	-0.15511316313064327
2215	38	-0.16480482396912066
2215	39	-0.18444450873663953
2215	40	-0.4684081530594355
2215	41	-0.3594077671777782
2215	42	-0.4607517608643761
2215	43	-0.6221322926660378
2215	44	-0.29610535252784587
2215	45	-0.6097970159863237
2215	46	-0.37637218168803344
2215	47	-0.6291406648838489
2216	1	-0.14696903263281058
2216	2	1.431195419228061
2216	3	-0.04643973239667787
2216	4	-0.5162315850566088
2216	5	0.6106581235980713
2216	6	0.06370421243128277
2216	7	-0.5419478607318027
2216	8	-0.5262352058237383
2216	9	0.027489511279000567
2216	10	-0.3375254894050528
2216	11	-0.07981617108740835
2216	12	-0.45051843845351414
2216	13	0.04323199417940771
2216	14	-0.04587316058492519
2216	15	-0.0513285283771836
2216	16	-0.19606509248752246
2216	17	-0.027922147484844118
2216	18	0.2831043922768721
2216	19	0.15847150538930066
2216	20	0.6186189985159238
2216	21	0.7806429938508757
2216	22	0.7608869028970346
2216	23	0.6393984455143737
2216	24	0.5890982312424953
2216	25	0.5796222291691109
2216	26	0.7067710939972685
2216	27	0.3965117921648087
2216	28	0.6265326263332369
2216	29	0.4262089254416779
2216	30	0.23936371780369825
2216	31	-0.01464582220606303
2216	32	-0.10029844379186414
2216	33	0.019063889986263405
2216	34	0.14827810838550448
2216	35	0.011553054583423261
2216	36	-0.09613607351563047
2216	37	-0.10082168847626605
2216	38	0.0774186783350288
2216	39	0.30173060647073413
2216	40	-0.26895538808247466
2216	41	-0.046907767177778226
2216	42	0.11686920687755986
2216	43	-0.1254445046476036
2216	44	-0.08340373962462166
2216	45	0.15489192410583996
2216	46	-0.09224199735623273
2216	47	0.016884680738272095
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	1.0
25	2.0
26	32.0
27	128.0
28	370.0
29	811.0
30	1772.0
31	3547.0
32	5439.0
33	7254.0
34	10442.0
35	17556.0
36	25869.0
37	25873.0
38	39626.0
39	2702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	98.22945023864239	0.3167756761534382	0.43485946614813503	1.0189146190560368
2	1.091760180451553	96.17106128423241	1.653903537614108	1.0832749977019296
3	0.5607251951578233	0.5946656861635932	0.2991005769883471	98.54550854169024
4	1.3159104247541065	0.24394900405167477	97.99466847683898	0.44547209435523216
5	1.1200282835425137	94.66360261622768	1.2996287785045075	2.916740321725296
6	0.5882976842849567	0.13505391550291673	0.4680926286017324	98.8085557716104
7	98.68127983029873	0.7898179246950681	0.29485593070532085	0.2340463143008662
8	1.7289415624823217	1.2693047462804774	95.344232618657	1.657521072580189
9	19.972705030477027	33.99072253885644	28.184530964940812	17.85204146572572
10	20.960556647668614	31.58296680761996	17.77425787382087	29.682218670890553
11	22.462328258579117	33.65460086692736	15.821070718927444	28.062000155566086
12	29.041541453066998	32.04101113664486	19.201697012550824	19.715750397737317
13	20.207462718228296	35.106029429442174	28.52577020710917	16.160737645220365
14	21.759236344352132	42.72582640975782	17.173767014318546	18.341170231571503
15	33.998939367155735	19.958988863355135	18.01944493547817	28.02262683401096
16	23.039066643097048	32.62153084673856	17.822167226445114	26.517235283719288
17	30.940074244299097	32.88951741205586	18.747746155205938	17.4226621884391
18	33.75129951414084	26.70528080113721	16.177625018564488	23.36579466615747
19	26.0417329571569	27.030256747488036	24.32489764748308	22.60311264787199
20	34.617179545197416	23.966229211449257	22.512444846702113	18.904146396651207
21	27.540392434152377	29.167403217252964	24.687290083082907	18.604914265511756
22	18.89535500385374	26.320702017380725	18.76453991981389	36.019403058951646
23	19.44961136140207	28.56758916762736	19.98854241076165	31.994257060208923
24	24.529769480978644	26.65323150898034	25.228397680667513	23.5886013293735
25	21.06204702138943	29.783277355488774	23.81262153084674	25.342054092275056
26	22.645218313593777	24.4426374403394	23.437157503977375	29.47498674208945
27	24.630015909492663	25.78257026692593	28.704260208591126	20.883153614990277
28	21.40781332861941	30.01732366978964	27.002298037829238	21.57256496376171
29	20.906487537564082	23.775145837016087	31.95474633197808	23.363620293441752
30	19.71801108722706	25.12727684127164	29.173973300147075	25.98073877135423
31	20.33728124447587	25.300335867067353	31.18189853279123	23.18048435566555
32	21.50963408166873	26.500265158211068	26.36026162276825	25.62983913735195
33	25.24942549054269	27.681810146720874	25.81580342937953	21.252960933356903
34	21.74155913028107	31.666961286901184	26.422485416298393	20.168994166519358
35	22.069648223439987	29.479936362029346	28.132225561251545	20.318189853279122
36	22.893508697496816	28.211002687031538	28.072408428793665	20.82308018667798
37	23.49938129750751	27.428672441223263	27.599787873431147	21.47215838783808
38	23.38570539653807	28.007268921823737	29.179630048648036	19.42739563299016
39	21.681456602439457	28.760120205055685	29.45730952801838	20.101113664486476
40	23.030581580342936	28.33233162453597	28.021212656885275	20.615874138235814
41	22.223243579590452	28.902449372100918	28.608298450050913	20.26600859825772
42	21.450945730952803	27.515644334452887	29.226798656531734	21.806611278062576
43	22.590772494254903	28.330917447410286	28.543044016263035	20.535266042071772
44	22.90328967838656	26.765055299408818	27.39159335841371	22.94006166379091
45	21.033129441714106	28.48919845843793	27.27292012869922	23.204751971148745
46	24.870426020859114	26.658652996287785	27.644334452890224	20.826586529962878
47	23.265450431339275	25.588318483948523	29.876255126573326	21.269975958138875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	3.0
8	4.0
9	4.0
10	12.5
11	21.0
12	40.0
13	59.0
14	101.5
15	144.0
16	218.5
17	293.0
18	377.0
19	461.0
20	938.0
21	1415.0
22	1260.5
23	1106.0
24	1256.5
25	1407.0
26	1407.0
27	1629.5
28	1852.0
29	2043.0
30	2234.0
31	2453.0
32	2672.0
33	2886.5
34	3101.0
35	3577.5
36	4054.0
37	4458.0
38	4862.0
39	5617.0
40	6372.0
41	7803.0
42	9234.0
43	9234.0
44	12183.0
45	15132.0
46	16053.5
47	16975.0
48	17957.5
49	18940.0
50	16790.5
51	14641.0
52	11977.0
53	9313.0
54	8171.5
55	7030.0
56	6384.5
57	5739.0
58	5739.0
59	5099.5
60	4460.0
61	3455.0
62	2450.0
63	2845.0
64	3240.0
65	2883.5
66	2527.0
67	1649.0
68	771.0
69	546.0
70	321.0
71	265.0
72	209.0
73	172.0
74	135.0
75	135.0
76	153.5
77	172.0
78	111.5
79	51.0
80	36.0
81	21.0
82	11.5
83	2.0
84	2.5
85	3.0
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0014141771256849921
3	7.070885628424961E-4
4	0.0014141771256849921
5	0.0
6	0.0
7	0.0
8	0.006363797065582464
9	0.004949619939897472
10	0.004949619939897472
11	0.004242531377054976
12	0.0
13	0.0014141771256849921
14	0.0
15	0.0
16	0.0
17	0.0
18	0.018384302633904896
19	0.0014141771256849921
20	7.070885628424961E-4
21	0.0
22	0.0028283542513699842
23	0.024041011136644867
24	0.00353544281421248
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	7.070885628424961E-4
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.00353544281421248
37	0.0
38	7.070885628424961E-4
39	0.0
40	0.0
41	7.070885628424961E-4
42	0.0
43	0.0
44	0.009192151316952448
45	0.00707088562842496
46	0.0
47	0.00353544281421248
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
47	141425.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.32296270107831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.4621554522885	20.147781509634083
2	16.09056379570098	10.723705144069294
3	8.163048783075519	8.160509103765246
4	4.9398434018715385	6.584408697189323
5	2.8667218367390244	4.776383242001061
6	1.7993931292040657	3.5976666077426196
7	1.2498143314872578	2.9153261445996113
8	0.7532836802682115	2.0081315184726884
9	0.6153584993740319	1.8455011490189146
>10	2.571774142211471	15.10694714512993
>50	0.24189954802979183	5.738730776029698
>100	0.23341184459015002	13.83560190913912
>500	0.006365777579731364	1.2897295386247127
>1k	0.006365777579731364	3.269577514583702
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GACTACGTAACGTACAACGGCCGACGATCTCAAAACGTAGTCACTGT	2033	1.4375110482587943	No Hit
GACTACGTAACGTACAACCGGCCGACGATCTCAAAACGTAGTCACTG	1401	0.9906310765423368	No Hit
GACTACGTTCAAAAGCGACGACTCACTATAGGGAGTCGTATTACCGG	1190	0.8414353897825702	No Hit
GACTACGTACCTTTCACACGTTTTTTTTTTTTTTTTTTTTTTTTTTT	667	0.4716280714159448	No Hit
GACTACGTAACGTACAATACGACTCCACTATAGGGAGTCGTATTACC	583	0.4122326321371752	No Hit
CACGACGTGTCACCCGAAGGAGCTCCTAGCCCAGTTGCAGGGTGCTC	574	0.4058688350715927	No Hit
GACTACGTAACGTACAAGGCCGACGATCTCAAAACGTAGTCACTGTT	410	0.28990631076542334	No Hit
GACTACGTTCAAAAGCGGAGTCGTATTACCGGCCTCAATCAAAAACG	383	0.270814919568676	No Hit
GACTACGTCCACTATGACAGAGCGGTGGCGGCCATGGGGACGGAGGC	371	0.26232985681456605	No Hit
GACTACGTAAGAGCGGACCATCACAATGCTTCATGATGACAGGACAA	345	0.24394555418066113	No Hit
GACTACGTTCAAAAGCGAGTGAGTCGTATTACCGGCCTCAATCAAAA	341	0.24111719992929115	No Hit
GACTACGTAACGTACAAAGTGAGTCGTATTACCGGCCGACGATCTCA	335	0.23687466855223616	No Hit
GACTACGTTCAAAAGCGAGTCGTATTACCGGCCTCAATCAAAAACGT	320	0.22626834010959873	No Hit
GACTACGTAACGTACAAACCGGCCGACGATCTCAAAACGTAGTCACT	313	0.22131872016970125	No Hit
GACTACGTTCAAAAGCGGTCGTATTACCGGCCTCAATCAAAAACGTA	293	0.20717694891285135	No Hit
GCCTACGTATGTAGGATCCTACATTCGAGATCGTCGGACTGTAGAAC	286	0.20222732897295387	No Hit
GACTACGTTCAAAAGCGCGGCCTCAATCAAAAACGTAGTCACTGCGC	267	0.18879264627894643	No Hit
GAGTTCGTAATCGGTACGGACGTCGTCCGCGAGGAGGTCGCGCGGAT	261	0.18455011490189147	No Hit
GACTACGTTCAAAAGCGTCAAAAACGTAGTCACTGCGCTTTTGATCG	254	0.17960049496199398	No Hit
GACTACGTTCAAAAGCGGAACTCTGAACCCCTATAGTGAGTCGTATT	253	0.1788934063991515	No Hit
GACTACGTTGACGCTAGGTGACATACTTTCCTTTTGGTAAAGCAAAG	248	0.17535796358493902	No Hit
GACTACGTTCAAAAGCGACTCTGAACCCCTATAGTGAGTCGTATTAC	236	0.16687290083082906	No Hit
GACTACGTTCAAAAGCGAAAACGTAGTCACTGCGCTTTTGATCGAGA	228	0.1612161923280891	No Hit
GACGACGAAGGATTATGGCGCCGCCAGCCATGGCCGACCATAGACGC	224	0.1583878380767191	No Hit
GACTACGTTCAAAAGCGATTACCGGCCTCAATCAAAAACGTAGTCAC	224	0.1583878380767191	No Hit
GACTACGTTCAAAAGCGTGAGTCGTATTACCGGCCTCAATCAAAAAC	223	0.15768074951387662	No Hit
GACTACGTTCAAAAGCGACCCCTATAGTGAGTCGTATTACCGGCCTC	220	0.15555948382534912	No Hit
GACTACGTATGTAGGATCCTACATTCGAGATCGTCGGACTGTAGAAC	220	0.15555948382534912	No Hit
GACTACGTTCAAAAGCGGTCGGACTGTAGAACTCTGAACCCCTATAG	219	0.15485239526250663	Illumina NlaIII expression Adapter 1 (95% over 24bp)
GACTACGTTCAAAAGCGCGTATTACCGGCCTCAATCAAAAACGTAGT	218	0.15414530669966411	No Hit
GACTACGTTCAAAAGCGGTAGAACTCTGAACCCCTATAGTGAGTCGT	216	0.15273112957397914	No Hit
GACTACGTTCTGGGTCGGGCAAAAAGTGAGTGGAGATTGGATACAGC	215	0.15202404101113665	No Hit
GACTACGTTCAAAAGCGATCAAAAACGTAGTCACTGCGCTTTTGATC	214	0.15131695244829413	No Hit
GACTACGTAGCCCTTGTACTGGAGCCAGCCGGGCACGGTGGCTCACA	214	0.15131695244829413	No Hit
GACTACGTAACGTACAAGCCGACGATCTCAAAACGTAGTCACTGTTG	212	0.14990277532260915	No Hit
GACTACGTAACGTACAAACTCTGAACCCCTATAGTGAGTCGTATTAC	209	0.14778150963408168	No Hit
GACTACGTTCAAAAGCGCTCTGAACCCCTATAGTGAGTCGTATTACC	207	0.14636733250839667	No Hit
GACTACGTAACGTACAAGTAGAACTCTGAACCCCTATAGTGAGTCGT	206	0.14566024394555419	No Hit
GACTACGTTCAAAAGCGAGAACTCTGAACCCCTATAGTGAGTCGTAT	206	0.14566024394555419	No Hit
GACTACGTTCAAAAGCGGGACTGTAGAACTCTGAACCCCTATAGTGA	203	0.1435389782570267	Illumina DpnII expression Sequencing Primer (95% over 21bp)
GACTACGTAACGTACAAGAACCCCTATAGTGAGTCGTATTACCGGCC	200	0.14141771256849922	No Hit
GACTACGTTCAAAAGCGACCGGCCTCAATCAAAAACGTAGTCACTGC	199	0.1407106240056567	No Hit
GACTACGTAACGTACAAGAGTCGTATTACCGGCCGACGATCTCAAAA	198	0.14000353544281421	No Hit
GACTACGTTCAAAAGCGAACCCCTATAGTGAGTCGTATTACCGGCCT	198	0.14000353544281421	No Hit
GACTACGTTCAAAAGCGTCGTATTACCGGCCTCAATCAAAAACGTAG	197	0.13929644687997172	No Hit
GACTACGTTCAAAAGCGGAACCCCTATAGTGAGTCGTATTACCGGCC	196	0.13858935831712924	No Hit
GACTACGTCAGGAATAGTAGGAAGGTTTAGGACATGGCTCAAGGCGA	194	0.13717518119144423	No Hit
GACTACGTTCAAAAGCGGTATTACCGGCCTCAATCAAAAACGTAGTC	193	0.13646809262860174	No Hit
GACTACGTAACGTACAAGAACTCTGAACCCCTATAGTGAGTCGTATT	192	0.13576100406575922	No Hit
GACTACGTTCAAAAGCGTACCGGCCTCAATCAAAAACGTAGTCACTG	183	0.12939720700017676	No Hit
GACTACGTAACGTACAAAGTCGTATTACCGGCCGACGATCTCAAAAC	182	0.12869011843733427	No Hit
GACTACGTATGTTTTTGTTTTTGTGGGAGCGAAGCGGTGGCTGGGCT	176	0.12444758706027931	No Hit
GACTACGTAACGTACAAAGAACTCTGAACCCCTATAGTGAGTCGTAT	173	0.12232632137175181	No Hit
GACTACGTATCCAGGTTAGTCCGACGATCTCGAAAGGTCTGTAGAAC	171	0.12091214424606682	No Hit
GACTACGTCAAGAGATGGGCATTGAGGCAGCCAGCGCAGGGGCTTCT	170	0.12020505568322432	No Hit
GACTACGTTCAAAAGCGCTATAGTGAGTCGTATTACCGGCCTCAATC	169	0.11949796712038183	No Hit
GACTACGTCCTACCTACGCGCACGGAGTTTCTGCAGCGGGAATCCGA	169	0.11949796712038183	No Hit
GACTACGTAACGTACAATGAGTCGTATTACCGGCCGACGATCTCAAA	169	0.11949796712038183	No Hit
GACTACGTAAGGACATGAGGAGGGTGGATGTCTGCGAGTGCTGTCTT	167	0.11808378999469685	No Hit
GACTACGTTCAAAAGCGTGTAGAACTCTGAACCCCTATAGTGAGTCG	166	0.11737670143185433	No Hit
GACTACGTTCAAAAGCGAACTCTGAACCCCTATAGTGAGTCGTATTA	165	0.11666961286901185	No Hit
GACTACGTTCAAAAGCGAACGTAGTCACTGCGCTTTTGATCGAGATC	161	0.11384125861764186	No Hit
GTATACGTGAAGGACACGCGGTTCGCTCATCGCCATCGTCATGTCGC	159	0.11242708149195686	No Hit
GACTACGTTCAAAAGCGCGTCGGACTGTAGAACTCTGAACCCCTATA	158	0.11171999292911437	Illumina DpnII expression Sequencing Primer (95% over 24bp)
GACTACGTAACGTACAAAAACGTAGTCACTGTTGTACGTTTCGAGAT	154	0.1088916386777444	No Hit
GACTACGTAACGTACAAGGACTGTAGAACTCTGAACCCCTATAGTGA	154	0.1088916386777444	Illumina DpnII expression Adapter 1 (95% over 21bp)
GACTACGTCGACGTCCGCCGGCCGACGATCTCGACGTAGTCACTGCG	152	0.10747746155205941	No Hit
GACTACGTTCAAAAGCGTCAATCAAAAACGTAGTCACTGCGCTTTTG	151	0.1067703729892169	No Hit
GACTACATTTCCGTTTTGCTGAATCTATTTCAGTTCATGTGCTGAAT	150	0.10606328442637442	No Hit
GACTACGTAACGTACAAACCCCTATAGTGAGTCGTATTACCGGCCGA	148	0.10464910730068941	No Hit
GACTACGTATGATAATGGGGCAAGCTTCTAGCCTGTATTGCCTCACG	143	0.10111366448647693	No Hit
GACTACGTTCAAAAGCGCTGTAGAACTCTGAACCCCTATAGTGAGTC	142	0.10040657592363443	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0021212656885274883	0.0	0.0
17	0.0	0.0	0.07353721053561958	0.0	0.0
18	0.0	0.0	0.18808555771610394	0.0	0.0
19	0.0	0.0	0.3662718755524129	0.0	0.0
20	0.0	0.0	0.440516174650875	0.0	0.0
21	0.0	0.0	0.5006187024924872	0.0	0.0
22	0.0	0.0	0.5593070532084143	0.0	0.0
23	0.0	0.0	0.6031465441046491	0.0	0.0
24	0.0	0.0	0.7304224854162984	0.0	0.0
25	0.0	0.0	0.808202227328973	0.0	0.0
26	0.0	0.0	0.9064875375640798	0.0	0.0
27	0.0	0.0	1.0245713275587767	0.0	0.0
28	0.0	0.0	1.160332331624536	0.0	0.0
29	0.0	0.0	1.8433798833303872	0.0	0.0
30	0.0	0.0	2.3461198515114017	0.0	0.0
31	0.0	0.0	2.618348948205763	0.0	0.0
32	0.0	0.0	3.0418949973484177	0.0	0.0
33	0.0	0.0	3.3749337104472334	0.0	0.0
34	0.0	0.0	3.656354958458547	0.0	0.0
35	0.0	0.0	4.215662011666962	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGGTT	20	0.0011059917	41.021587	17
GAGGTCT	20	0.0011059917	41.021587	18
ATACGTT	20	0.0011059917	41.021587	17
GATTCCA	20	0.0011059917	41.021587	18
CGGACGT	40	1.5272235E-8	41.021587	17
GCTATAG	30	4.0676423E-6	41.021584	17
GCGAGAA	20	0.0011079195	41.007076	15
CTTCGTT	20	0.0011079195	41.007076	3
CTTCGTA	40	1.5319529E-8	41.007076	3
GTTGACG	60	0.0	41.007076	7
CGTGGGC	30	4.077556E-6	41.007076	6
CTATGGA	20	0.0011079195	41.007076	3
AGCGTCG	35	2.49378E-7	41.007076	14
CGTGGCT	20	0.0011079195	41.007076	6
AGCGTAG	55	3.6379788E-12	41.007076	14
CGGAACT	30	4.077556E-6	41.007076	16
GTCGCTC	20	0.0011079195	41.007076	7
GTCGCTA	60	0.0	41.007076	7
GTACCTT	95	0.0	41.007076	7
CGGCGCA	20	0.0011079195	41.007076	12
>>END_MODULE