FastQCFastQC Report
Thu 26 May 2016
SRR2099180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559448
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGATCCTAGTACTAGTGACGATCTTTTTTTTTTTTTTTTTTTTT43120.7707597488953397No Hit
GGACGATCATCACGTCTGGCTGGTTGCCGGACGCGAACGCGAACCAC15560.27813130085369864No Hit
TGACGATCGACGAATATAGGATGAGTTTTACTCATCCTTTTTTTGTG15200.2716963864380604No Hit
TGCCGTTCCCAGCGGCTGGAGCAGGCCAACGGTCTGATGAACAGCGC13200.23594686190673664No Hit
TGACGATCTCTCCCAGACACCTGCCATGACCTCCACCCCATTAATTA10980.19626488967696729No Hit
TGACGATCCGAATCAGAGAGTTTGAAATAGGGAAATAATACAGCAAC7370.13173699789792798No Hit
TGACGATCGTTTATGGAAAACAGTCCCTCTGCCCTGGACCCCCGGCA6740.12047589767056098No Hit
TGACGATCCGTAATAGACAGGCATGGTGGTACATGCTTGTAGTCCCA6730.12029715004790435No Hit
TGACGATCCGGTAATGGGGCGCCGTCCGCATCGCCCGGCCAAAATGA6590.11779468333071169No Hit
TGACGATCTGTTACCTACCTGGCACAGTGTGTTTTCTGGACCAATTC6470.11564971185883229No Hit
TGACGACTTCTTTCTAACAGTTTTCCTCGCCATATTTCACGCCCTAA6450.11529221661351904No Hit
GGACGATCATCACGTCTGGGCTGGTTGCCGGACGCGAACGCGAACCA6190.11064477842444695No Hit
TGACGATCTGAGCGAACATGGACAGATGGGGAATGGATGGATAGATG5920.10581859261271824No Hit
TGACGATCAGTCTAAGCGCGGCGCGTGCGCGGGCGGGGCCCGCGTGC5790.1034948735181822No Hit
TGACGACTTCTTTCTAAAATATGGCAGTTTTCCTCGCCATATTTCAC5740.10260113540489911No Hit
TGACCCTCTTGCCTGGGGGCAGGCCTTCATTCTCGCCGATAACGCGC5740.10260113540489911No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACGC256.717698E-541.017277
TCCGTAT505.820766E-1141.017277
GCTCCGT304.0923132E-641.017275
TCGGAAT750.041.0172657
TCCGTTG459.531504E-1041.0172657
CTTCGGC200.001109866541.01368
ACTGCCG304.0948307E-641.01369
CGTTAAC1000.041.01368
CTGTGGG256.721243E-541.01368
CGTTTAG650.041.0135969
ACACGGA200.001112800640.99159614
ACACGCG304.109963E-640.99159612
GCGAATC200.001112800640.99159614
TTATAGG200.001112800640.99159612
TACTCGA352.518973E-740.99159614
GCGGCTT200.001112800640.99159612
TAGATAC200.001112800640.99159614
TTACGGG256.742546E-540.99159612
TGAGCGG200.001112800640.99159612
TACAACG304.109963E-640.99159614
Produced by FastQC (version 0.11.5)