FastQCFastQC Report
Thu 26 May 2016
SRR2099179_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099179_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100158
Sequences flagged as poor quality0
Sequence length47
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGATCCTAGTACTAGTGACGATCTTTTTTTTTTTTTTTTTTTTT11741.1721480061502825No Hit
GGACGATCATCACGTCTGGCTGGTTGCCGGACGCGAACGCGAACCAC4600.45927434653247873No Hit
TGCCGTTCCCAGCGGCTGGAGCAGGCCAACGGTCTGATGAACAGCGC4460.4452964316380119No Hit
TGACGATCGACGAATATAGGATGAGTTTTACTCATCCTTTTTTTGTG4270.42632640428123564No Hit
TGACCCTCTTGCCTGGGGGCAGGCCTTCATTCTCGCCGATAACGCGC3040.3035204377084207No Hit
TGACGATCCGGTATGGGGTGGCTTCAAAGAAGCTATTGATTCTGAAG2700.26957407296471575No Hit
TGACGATCTCTCCCAGACACCTGCCATGACCTCCACCCCATTAATTA2650.2645819605024062No Hit
TGACGATCTGAGCGAACATGGACAGATGGGGAATGGATGGATAGATG2050.2046766109546916No Hit
TGACGATCTGTTACCTACCTGGCACAGTGTGTTTTCTGGACCAATTC2010.20068292098484394No Hit
TGACCCTCTTGCCTGGGGGGCAGGCCTTCATTCTCGCCGATAACGCG1860.18570658359791528No Hit
TGACGATCCGTAATAGACAGGCATGGTGGTACATGCTTGTAGTCCCA1840.18370973861299147No Hit
GGACGATCATCACGTCTGGGCTGGTTGCCGGACGCGAACGCGAACCA1820.18171289362806767No Hit
TGACGATCCGGTAATGGGGCGCCGTCCGCATCGCCCGGCCAAAATGA1790.1787176261506819No Hit
TGGCAATCTTCTTAACTGATCCGCCTCGGCCAGGTCATTAGTGGCTG1740.17372551368837238No Hit
TGACGATCACATGACGAGTGACGATCTTTTTGCCATGGATCGTACTG1690.1687334012260628No Hit
GGACGCTCTCGACGCGATCGAAGGCTACAGCGGCGCGAGATTTCATG1620.16174444377882946No Hit
TGACGACTTCTTTCTAACAGTTTTCCTCGCCATATTTCACGCCCTAA1620.16174444377882946No Hit
TGACGACTTCTTTCTAAAATATGGCAGTTTTCCTCGCCATATTTCAC1420.14177599392959125No Hit
TGACGCTGAAGACAACGGCGGCCGCCCATTCGCCGCCGAAGCCGAGG1410.14077757143712935No Hit
TGACGATCTTGTCTCCAGTCCGACGATCCATGAGGTAGTCGTACTGT1410.14077757143712935No Hit
TGACGATCGCGAAAGGTGCCTTCACCATGTACTAGACTAGATAACCT1370.1367838814672817No Hit
TGACGATCCGAATCAGAGAGTTTGAAATAGGGAAATAATACAGCAAC1260.1258012340502007No Hit
TGACGATCTTGTCTCCAGGTCCGACGATCCATGAGGTAGTCGTACTG1210.12080912158789112No Hit
GGACGCTCTCGACGCGATGAAATCTCGAAGGCTACAGCGGCGCGAGA1150.11481858663311968No Hit
TGACGACGAGTTCTTTACTGCCCGGTGCGAGCCCGTGCGGACGCACC1140.11382016414065775No Hit
TGACGATCATAAACGTACAGCATGCCGGGTCCAACGCCCAGTGGCAC1130.11282174164819585No Hit
TGACGATCAATGGCAACGTCCGACGATCCATGTCTGGTAAGTCAGAC1130.11282174164819585No Hit
TGACGATCGGCAAACCGGTCCGACGATCCATGAGGGCATTGTCAGAC1070.10683120669342439No Hit
TGACGATCCATGAGGAGCAGTCCGACGATCCATGGATCGTCGGACTG1050.10483436170850056No Hit
TGACGATCCGCTCAGTTAACCAAACCAAACCAAAAAAACAAACAAAC1030.10283751672357676No Hit
TGACGATCATTGCTATGGTCCGACGATCCATGGACGGTAGTTCAGTG1010.10084067173865292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGG750.041.00000413
GATCACT1100.041.0000045
TCGGCAA553.6379788E-1241.0000047
CTGCAAT553.6379788E-1241.0000048
AGTTCTA1350.041.00000419
TATAGGA553.6379788E-1241.00000415
ATCGCAT553.6379788E-1241.0000046
ACGACTT1100.041.0000043
ATCTGTA553.6379788E-1241.0000046
CTTAATG200.001107310441.019
TCGCCGT200.001107310441.07
CTTAACA256.694784E-541.08
TTTAGCT256.694784E-541.011
TTCTAAT352.4874134E-741.012
TGTCACA1050.041.09
GGGTGTC200.001107310441.012
CTATGGG200.001107310441.013
TCGCATA256.694784E-541.07
ATCTCGG200.001107310441.06
GAAGCTA256.694784E-541.029
Produced by FastQC (version 0.11.5)