FastQCFastQC Report
Thu 26 May 2016
SRR2099178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1321849
Sequences flagged as poor quality0
Sequence length47
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATCCTCATGGTGTTGCCGATCGAGATCATCGCGACCCAGCCCAG30910.23383911475516492No Hit
CTGCTGCACCTGGCGCACATCACCGGCTGCGGACTGCGGTGCCAGGA26180.19805590502394751No Hit
CTGATGCATTATGTATAGGTGGACATGTGGCCCAAATTTATGCCATC16240.122858208464053No Hit
CTTATGGACACAAAACCGTGTAGTGAGACCTGGGATGCAGGACTGGA15220.11514174463195116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCAC245150.040.789853
ATGCACG68850.040.6484034
ATGCACC54600.040.254734
GATGCAG289500.040.206993
TGCAACG22050.040.0759165
TGCACGG20250.039.9932635
ATGCACA82500.039.788014
ATGCAGC62800.039.6345444
ATGCACT49200.039.6305584
ATGCAAC80400.039.6286964
TGCACGA24100.039.5594945
ATGCAAG103900.039.545494
GATGCAA417900.039.5059623
TGCAGCG17250.039.341755
ATGCAAT81100.039.337214
CATAACG1150.039.2228977
ATGCAGG79900.039.004224
ATGCAGA92800.038.906864
TGCACAC19400.038.8920565
TGCAAGG26900.038.8716245
Produced by FastQC (version 0.11.5)