FastQCFastQC Report
Thu 26 May 2016
SRR2099174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153152
Sequences flagged as poor quality0
Sequence length42
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGAGAATCAATCCTCGGCGCGGTGATGCGGCTCGCGGGC19011.2412505223568742No Hit
CTACGAGTATCTTAGGTTTGGTCTTCTCATTTTGTCCTGTAT13840.9036773923944839No Hit
CTACGAGTGGGTATGGCAGCAGCTGTGACGATGGACTGTCAT9310.6078928123694107No Hit
CTACGAGTCTGTGAAGCGGAGCGTTCCGTTCCCGGCTTCAGG8890.5804690764730465No Hit
CTACGAACCTGACCGGCGGCGACCGCCGGCAGTTCGTGGTTT7540.492321353949018No Hit
CTACAAGTGGGAGCAGCTGGATCATTTCATCATTAGACAAAA7230.4720800250731299No Hit
CTCCGAGGAGCTGGGAAATGGGTGTTTGTTCCATCGTGGGAG7100.46359172586711245No Hit
CTAGAAGTATCATATTCTCGGGACGAAGGCTGCATATATAAC6950.4537975344755537No Hit
CTACGAGTCTGCAGCAAACAGGATTAGATACCCTGGTAGTCC6290.4107030923526954No Hit
CTACGAGTACATAGCCCCATCCCTGTCCATGGAAAAACTGTC5670.37022043460091936No Hit
CTACGCGTTGCAGGATTCTCGAAGAGAAAACACGTGATTTTC4940.32255536982866695No Hit
CTACGAGTTCGAGGCTGAGAGAAGATGTGATGATCCAGATAA4670.30492582532386125No Hit
CTGCGTGTTCAGGCTGGAGTTAAAATGGTTATGATTCAAGAC4590.2997022565816966No Hit
CTACGACTGGTAAATAGGACCGCGGTTCTATTTTGTTGGTTT4350.28403155035520267No Hit
CTACGACCCTCGGAAGTGTTGGTGGTTACAGTTATACATCGG3910.2553019222732971No Hit
CTACGAGTCCACGAATCACCCAGAACCGCTAAGCCACCAGAA3740.24420183869619722No Hit
CTAAGAGCGTACATCGTACTCTTGCAGTGTCAACTGCTAGAG3580.23375470121186795No Hit
CTGCGGGTCTGGCTACGTCGGCGCCGAAGGCCGCCGGCGTAG3510.22918407856247386No Hit
CTACGAGTTAGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCT3390.2213487254492269No Hit
CTACGAGTATACGCCGGCGCCTAGCAGCCGACTTAGAACTGG3060.19980150438779773No Hit
CTACGAGTTCGAGCTGAGAGAAGATGTGATGATCCAGATAAC2610.17041893021312163No Hit
CTACGAGTCTTTGCCGCCGAAGGAGGGGCAAAGCTCAAGATG2540.16584830756372754No Hit
CTACGAGTGCGTTCCGACGATCAGTCCTACGAGTTTTTTTTT2480.16193063100710406No Hit
CTACGAGAATCACATCCTCGGCGCGGTGATGCGGCTCGCGGG2370.15474822398662766No Hit
CTACGAGTCCCATTCTCTTTCAATTGATTCCTTACCTCTCTC2360.1540952778938571No Hit
CTACGAGAATCAGATCCTCGGCGCGGTGATGCGGCTCGCGGG2310.15083054743000418No Hit
CTACGAGAATCATATCCTCGGCGCGGTGATGCGGCTCGCGGG2270.14821876305892184No Hit
CTACGAGTACAATCGGAACAAGAAGAGGGTAAGGTGGCTCAG2250.1469128708733807No Hit
CTACGAGTCTGTGGAAGCGGAGCGTTCCGTTCCCGGCTTCAG2190.1429951943167572No Hit
CTACGACTCGTGAATTATTCCCCATGAACGAGGAATTCCCAG2150.1403834099456749No Hit
CTGCGTGTTCAGGGCTGGAGTTAAAATGGTTATGATTCAAGA2150.1403834099456749No Hit
CTACGAGTTGTTGCAGAATTTGAATTCAAACGAATATTTTAT2140.1397304638529043No Hit
CTACGAGTACAACGGAACAAGAAGAGGGTAAGGTGGCTCAGC2120.13842457166736313No Hit
CTACGAGTAGCCCTCTTCAAGCAGAACTACAAACCACTGCTT2020.13189511073965732No Hit
CTGCGGGTAGTCTCAGTCTTGGAACAAAGCTTGCACTGATCG1960.12797743418303387No Hit
CTACGAGTCCTGATGGTACAGGTTGGGCGTTGTGGCTCATGC1940.1266715419974927No Hit
CTACGAGTACGTGAAAGAAAACAGCTTTGAAATGCAAAAAGT1890.12340681153363978No Hit
CTACGAGTCTGAAGCTGGTATTTGATATCTTTACTTGAAGGG1870.12210091934809861No Hit
CTACGCGTTGCAGGGATTCTCGAAGAGAAAACACGTGATTTT1650.10773610530714585No Hit
CTACGAGTATGATGAGGTACCCAGCTCTTGTGTGTGGGGGGT1600.10447137484329294No Hit
CTACGAGTCGGCGTGGGCTGCCCCTATAGGCAACTTCTTTGC1560.10185959047221062No Hit
CTACGAGTCGCAGAAATGCAAAAAGTTAGGTTTGCTATTTGC1550.10120664437944003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGA251.3946468E-436.21130836
TAATGAC200.00204187836.21130436
GGTAAAT700.036.0336849
CATGCAG200.002091789236.0336849
AGCCCTC200.002091789236.0336849
TGCGGCC200.002091789236.0336848
TGCGCAA551.6370905E-1136.0336848
TCTGTGG404.7921276E-836.0336848
TCGCTAT356.875707E-736.0336848
TCCACGG404.7921276E-836.0336848
TTGCGTC251.4356058E-436.0336848
TTGCGGT251.4356058E-436.0336848
CGTACAT251.4356058E-436.0336848
ATACGCC404.7921276E-836.0336849
TATACGC404.7921276E-836.0336848
TTCGATA251.4356058E-436.0336848
TAGCTCC200.002091789236.0336848
TTCGACT200.002091789236.0336848
TATCGAG200.002091789236.0336848
TGCCGAG251.4356058E-436.0336848
Produced by FastQC (version 0.11.5)