FastQCFastQC Report
Thu 26 May 2016
SRR2099173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44201
Sequences flagged as poor quality0
Sequence length42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGAGAATCAATCCTCGGCGCGGTGATGCGGCTCGCGGGC5531.2511029162236147No Hit
CTACGCGTTGCAGGATTCTCGAAGAGAAAACACGTGATTTTC3460.7827877197348476No Hit
CTACGAGTATCTTAGGTTTGGTCTTCTCATTTTGTCCTGTAT3320.7511142281848826No Hit
CTACGAGTCTGTGAAGCGGAGCGTTCCGTTCCCGGCTTCAGG2600.5882219859279202No Hit
CTACGAGTGGGTATGGCAGCAGCTGTGACGATGGACTGTCAT2380.5384493563494038No Hit
CTCCGAGGAGCTGGGAAATGGGTGTTTGTTCCATCGTGGGAG1950.44116648944594017No Hit
CTACAAGTGGGAGCAGCTGGATCATTTCATCATTAGACAAAA1950.44116648944594017No Hit
CTACGAACCTGACCGGCGGCGACCGCCGGCAGTTCGTGGTTT1940.4389040971923712No Hit
CTACGAGTACATAGCCCCATCCCTGTCCATGGAAAAACTGTC1840.41628017465668193No Hit
CTAGAAGTATCATATTCTCGGGACGAAGGCTGCATATATAAC1800.40723060564240626No Hit
CTACGAGTCTGCAGCAAACAGGATTAGATACCCTGGTAGTCC1760.39818103662813065No Hit
CTACGAGTCGGCGTGGGCTGCCCCTATAGGCAACTTCTTTGC1560.3529331915567521No Hit
CTACGAGTGGATGAGGCCCCTTGGTATTGCAAACTTTATATG1400.3167349154996493No Hit
CTGCGTGTTCAGGCTGGAGTTAAAATGGTTATGATTCAAGAC1360.3076853464853736No Hit
CTACGAGTTCGAGGCTGAGAGAAGATGTGATGATCCAGATAA1230.27827424718897764No Hit
CTACGACTGGTAAATAGGACCGCGGTTCTATTTTGTTGGTTT1200.27148707042827086No Hit
CTACGACCCTCGGAAGTGTTGGTGGTTACAGTTATACATCGG1160.2624375014139952No Hit
CTACGCGTTGCAGGGATTCTCGAAGAGAAAACACGTGATTTT1110.25112554014615057No Hit
CTGCGGGTCTGGCTACGTCGGCGCCGAAGGCCGCCGGCGTAG1080.24433836338544376No Hit
CTACGAGTGCGGGAAGGCAGATGTGTTTTTCATTCTGTTTTT1040.23528879437116806No Hit
CTACGAGTCCACGAATCACCCAGAACCGCTAAGCCACCAGAA990.22397683310332347No Hit
CTACGAGTGAGACAGTCCGACGATCCGGAACGATGTCTTTAA920.20814008732834097No Hit
CTACGAGTTGTCGGAGCCTTGGGGTGACTTCATGCCTCTTTT910.20587769507477205No Hit
CTACGAGTTCGAGCTGAGAGAAGATGTGATGATCCAGATAAC860.19456573380692746No Hit
CTACGAGTCTTTGCCGCCGAAGGAGGGGCAAAGCTCAAGATG810.18325377253908284No Hit
CTACGAGTTCCGGTGGCGCACGCCGGTAGGATTTGCTGAAGG800.1809913802855139No Hit
CTACGAGTACTTGAGACAGCAGGACTGTCCCCTCTAGGAGGT710.16062985000339358No Hit
CTACGAGAATCAGATCCTCGGCGCGGTGATGCGGCTCGCGGG690.15610506549625572No Hit
CTACGAGTCCCATTCTCTTTCAATTGATTCCTTACCTCTCTC680.1538426732426868No Hit
CTACGAGTGCTAAAGGCTGTATTTTACTCAGAAAATAATCTT680.1538426732426868No Hit
CTACGAGTAGGTGGGGAAGTGGCGAGGTCCACGCCCGGCTTG660.14931788873554896No Hit
CTACGAGTGTCCGGTGTCTGTGTCTGTCATTCCTAAAGTGCA660.14931788873554896No Hit
CTACGAGTTGTTGCAGAATTTGAATTCAAACGAATATTTTAT650.14705549648198005No Hit
CTACGAGTATACGCCGGCGCCTAGCAGCCGACTTAGAACTGG640.14479310422841113No Hit
CTACGAGAATCACATCCTCGGCGCGGTGATGCGGCTCGCGGG630.14253071197484218No Hit
CTGCGTGTTCAGGGCTGGAGTTAAAATGGTTATGATTCAAGA630.14253071197484218No Hit
CTACGAGAATCATATCCTCGGCGCGGTGATGCGGCTCGCGGG630.14253071197484218No Hit
CTACGAGTACGTGGGGCTGTGGCTGTTTCTGCTGCCCCTGGC590.1334811429605665No Hit
CTACGAGTCTGTGGAAGCGGAGCGTTCCGTTCCCGGCTTCAG580.1312187507069976No Hit
CTACGAGTCTCTGACGCCAGAGCCGAGCCCGCGTCGCCATGC560.12669396619985973No Hit
CTACGAGTCTGAAGCTGGTATTTGATATCTTTACTTGAAGGG550.12443157394629081No Hit
CTACGACTCGTGAATTATTCCCCATGAACGAGGAATTCCCAG530.11990678943915296No Hit
CTACGAGTACAATCGGAACAAGAAGAGGGTAAGGTGGCTCAG530.11990678943915296No Hit
CTACGAGTCCTGATGGTACAGGTTGGGCGTTGTGGCTCATGC500.1131196126784462No Hit
CTACGAGTTCTGGGAGCCATGGAGTTGCTCAAAGTGTCCAAG490.11085722042487725No Hit
CTACGAGTAGCCGTGGCGCACGCCTTTAATCCCAGCACTTGG490.11085722042487725No Hit
CTACGAGTACGGGGGCCCCAGAGCACTGGCATGGTGTGTGTG490.11085722042487725No Hit
CTACGAGTACGTGAAAGAAAACAGCTTTGAAATGCAAAAAGT490.11085722042487725No Hit
CTACGAGTGAATGAAGCCTGAGGTGGTGAAGACGCACCTGAG470.10633243591773943No Hit
CTACGAGTACAACGGAACAAGAAGAGGGTAAGGTGGCTCAGC460.10407004366417048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGAAG309.760475E-636.02831310
ACTCGTG404.6675268E-836.0283136
ACCGGCG356.7370456E-736.02831312
GGGTCTG309.760475E-636.0283135
GTATGAG251.4203685E-436.0283137
ACTCGCT251.4203685E-436.0283136
ATCTTAG251.4203685E-436.0283139
TAGTGCT200.00207725436.0283135
TATACGC200.00207725436.0283138
GAGTGTA404.6675268E-836.0283135
GAGTGGG1300.036.0283135
TGACCGG356.7370456E-736.02831310
GAGTGCG1350.036.0283135
ACCACTT309.760475E-636.0283133
GGGTATG453.243258E-936.0283139
CCAGTCC200.00207725436.02831312
CCAGTAT200.00207725436.0283134
AGTAGCT453.243258E-936.0283136
CAGTATT200.00207725436.0283135
GTTCTTG251.4203685E-436.0283137
Produced by FastQC (version 0.11.5)