##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099173_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 44201 Sequences flagged as poor quality 0 Sequence length 42 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.06278138503654 37.0 37.0 37.0 35.0 37.0 2 35.84534286554603 37.0 37.0 37.0 35.0 37.0 3 35.57405941042058 37.0 35.0 37.0 35.0 37.0 4 35.97350738671071 37.0 37.0 37.0 35.0 37.0 5 37.52231849958146 39.0 38.0 39.0 35.0 39.0 6 34.6930612429583 37.0 34.0 38.0 30.0 39.0 7 36.57498699124454 37.0 37.0 39.0 34.0 39.0 8 36.42971878464288 37.0 37.0 39.0 33.0 39.0 9 32.260107237392816 34.0 31.0 34.0 31.0 34.0 10 32.42347458202303 34.0 31.0 34.0 31.0 34.0 11 32.40363340195923 34.0 31.0 34.0 31.0 34.0 12 35.89699328069501 37.0 37.0 37.0 35.0 37.0 13 31.904187688061356 31.0 31.0 34.0 30.0 34.0 14 31.902875500554288 33.0 31.0 34.0 30.0 34.0 15 31.719938462930703 33.0 31.0 34.0 28.0 34.0 16 34.615280197280605 37.0 35.0 37.0 30.0 37.0 17 35.32508314291532 37.0 35.0 37.0 33.0 37.0 18 35.27058211352684 37.0 35.0 37.0 32.0 37.0 19 35.05859595936744 37.0 35.0 37.0 31.0 37.0 20 35.26773149928734 37.0 35.0 37.0 32.0 37.0 21 37.093572543607614 39.0 37.0 39.0 33.0 39.0 22 37.31646342842922 39.0 37.0 39.0 34.0 39.0 23 37.52829121513088 39.0 37.0 39.0 35.0 39.0 24 37.64313024592204 39.0 37.0 39.0 35.0 39.0 25 37.643401732992466 39.0 37.0 39.0 35.0 39.0 26 39.000407230605646 40.0 38.0 41.0 36.0 41.0 27 39.00585959593674 40.0 38.0 41.0 36.0 41.0 28 39.06836949390285 40.0 38.0 41.0 36.0 41.0 29 39.05938779665618 40.0 38.0 41.0 36.0 41.0 30 39.09454537227665 40.0 38.0 41.0 36.0 41.0 31 39.140788669939596 40.0 39.0 41.0 36.0 41.0 32 39.14710074432705 40.0 39.0 41.0 36.0 41.0 33 39.14250808805231 40.0 39.0 41.0 36.0 41.0 34 39.081536616818624 40.0 38.0 41.0 36.0 41.0 35 39.002646998936676 40.0 38.0 41.0 36.0 41.0 36 38.98771521006312 40.0 38.0 41.0 35.0 41.0 37 38.914096966131986 40.0 38.0 41.0 35.0 41.0 38 38.91432320535734 40.0 38.0 41.0 35.0 41.0 39 38.88138277414538 40.0 38.0 41.0 35.0 41.0 40 38.8879889595258 40.0 38.0 41.0 35.0 41.0 41 38.861496346236514 40.0 38.0 41.0 35.0 41.0 42 38.812017827650955 40.0 38.0 41.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16475984255126264 1101 2 0.3925603138628091 1101 3 0.253832542541538 1101 4 0.5572975081164344 1101 5 0.8526698262886541 1101 6 0.34594005397263317 1101 7 0.583090726397046 1101 8 1.034101651067438 1101 9 0.6909974738398859 1101 10 0.5517138613324448 1101 11 0.4576710378009352 1101 12 0.6528926359596028 1101 13 -0.5239481664388421 1101 14 0.07151814671467349 1101 15 -0.3203340362921878 1101 16 0.20405482033290667 1101 17 0.022570156267200048 1101 18 -0.581719323829823 1101 19 -0.684454195142095 1101 20 -0.31062764318745195 1101 21 -0.4511899560224535 1101 22 -1.2109494554577793 1101 23 -0.47114314088618414 1101 24 -0.8615109974023483 1101 25 0.3229887978365369 1101 26 -0.0953308070054959 1101 27 -0.03432255310338661 1101 28 -1.009022893906078 1101 29 -0.24870376128220784 1101 30 -0.21226694338848517 1101 31 -0.18688993468443726 1101 32 -0.09732554107614533 1101 33 -0.20393089085244043 1101 34 0.003804226781305431 1101 35 -0.010153738342978613 1101 36 -0.27431900245120033 1101 37 -0.050963429548012584 1101 38 -0.22307252696752045 1101 39 -0.7735287847769925 1101 40 -0.504143185652012 1101 41 -0.524447111630792 1101 42 -0.1934666636880351 1102 1 0.7737823989422381 1102 2 0.9486380080983992 1102 3 0.9568400613385251 1102 4 0.7935130469635538 1102 5 0.7220307285442971 1102 6 0.3077195025941819 1102 7 0.737226064742913 1102 8 1.146883605954656 1102 9 0.9353583760955289 1102 10 0.9191950643399593 1102 11 1.055102115495174 1102 12 0.8345968966112309 1102 13 0.6608889262929836 1102 14 0.5091747883186812 1102 15 0.49608200380806267 1102 16 0.7322503090547059 1102 17 0.665427299124346 1102 18 0.08870674133308398 1102 19 0.3246310178904892 1102 20 0.05779340944413036 1102 21 -0.3236836903582869 1102 22 0.10233375256226651 1102 23 0.1754734004671974 1102 24 0.26380228580567433 1102 25 0.23495621638289776 1102 26 0.6245940050245764 1102 27 -0.10512455811591792 1102 28 0.724686379276875 1102 29 0.6648300733042589 1102 30 0.5768934576140197 1102 31 0.7244509174458855 1102 32 0.5834388699514221 1102 33 0.550768357267863 1102 34 -0.017499031364060613 1102 35 0.2504978907296973 1102 36 0.3597662105813839 1102 37 0.2820566205773005 1102 38 0.5685941396991439 1102 39 0.8539774808871741 1102 40 0.46922774166628045 1102 41 0.5200391039080543 1102 42 1.057159902728003 1103 1 -0.18444650665508533 1103 2 -0.7046619083594123 1103 3 0.13478492349391757 1103 4 -0.7244485236295972 1103 5 -0.5302666816478521 1103 6 0.024511482544056662 1103 7 -0.8692902259839101 1103 8 -0.9024062854404988 1103 9 0.38147366431607566 1103 10 0.3076662422848244 1103 11 0.40013135526125154 1103 12 -0.02171053864357475 1103 13 0.04351215102147421 1103 14 0.2560419562384837 1103 15 0.3106183446601918 1103 16 0.5492929155710016 1103 17 0.33209396579101025 1103 18 0.15637591426541775 1103 19 0.5318156461277468 1103 20 0.05445172189191538 1103 21 0.11825448842199648 1103 22 0.5192092747009482 1103 23 0.19552352578048016 1103 24 0.45594932005797517 1103 25 0.41624276609050526 1103 26 0.24197078029609287 1103 27 0.27916951038867666 1103 28 0.4790723441891558 1103 29 0.2830422704638238 1103 30 0.24606638994484342 1103 31 0.203983081188575 1103 32 0.34116652241591794 1103 33 0.544084982163433 1103 34 0.36094708392415953 1103 35 0.6882589600697173 1103 36 0.4717127435805466 1103 37 0.6295921260075374 1103 38 0.2987528698578785 1103 39 0.20067756442936258 1103 40 0.42244411593528497 1103 41 0.34460050741682835 1103 42 0.6954222252008506 1104 1 0.18549716974020214 1104 2 0.5083437240010582 1104 3 -0.09409372473957234 1104 4 0.2151316094989255 1104 5 0.45462835163427684 1104 6 -0.0745668584697654 1104 7 0.40106307662745877 1104 8 0.3013827570582208 1104 9 -0.0901085169435234 1104 10 0.030400497277142335 1104 11 -0.18230592072441 1104 12 -0.08673870044592746 1104 13 -0.05275000975681721 1104 14 -0.14334360443740124 1104 15 0.10247702361564492 1104 16 0.6015202581209209 1104 17 -0.3507017331337181 1104 18 0.2615986485204118 1104 19 -0.15046802002688509 1104 20 -0.020305062542291807 1104 21 -0.4413973292943396 1104 22 0.3719998532979787 1104 23 -0.8881938321304261 1104 24 -0.05736353196455468 1104 25 -0.21099737727867307 1104 26 -0.4789713600008909 1104 27 0.026737354730713037 1104 28 -0.20372335473557257 1104 29 0.17756351982377794 1104 30 0.10858559117372124 1104 31 -0.04691297615909207 1104 32 -0.08407669314987487 1104 33 0.39457141329502576 1104 34 0.46693786733430187 1104 35 0.23408589299342708 1104 36 -0.05081669830373414 1104 37 -0.13333670143741472 1104 38 -0.04968543019332827 1104 39 0.2685219064672495 1104 40 -0.005871296251093838 1104 41 -0.037119922690699525 1104 42 -0.004526571522134759 1105 1 0.5821705568369779 1105 2 0.7608638852913785 1105 3 0.4033861139701074 1105 4 0.6309582224021497 1105 5 0.6406162548600847 1105 6 1.3079936254012026 1105 7 0.5094300121113307 1105 8 0.9001730796388685 1105 9 0.29144390241131646 1105 10 0.23251743276101422 1105 11 0.5424924663723658 1105 12 0.4475354931024569 1105 13 -0.22260888072455742 1105 14 -0.5601782818567571 1105 15 -1.0033697505779031 1105 16 -0.32942732252423923 1105 17 -0.39707270087565405 1105 18 -0.25805860954410775 1105 19 0.30215294771505086 1105 20 -0.12312764318745195 1105 21 0.19166718683469242 1105 22 0.2555684016850748 1105 23 0.042249716256669956 1105 24 0.29697114545479764 1105 25 -0.304243345020609 1105 26 -0.0752415212912112 1105 27 0.2580881611823287 1105 28 -0.10946932247750851 1105 29 0.05709981014636156 1105 30 -0.2926240862456311 1105 31 -0.7873363632558679 1105 32 -0.07500411250471473 1105 33 -0.13919874799529453 1105 34 0.13326851249559013 1105 35 0.15948911879987548 1105 36 -0.14708685959405443 1105 37 -0.4281955724051585 1105 38 -0.09807252696752045 1105 39 0.05682835808015341 1105 40 0.1811246714908421 1105 41 0.12064217408349265 1105 42 -0.03275237797375041 1106 1 0.694430172221594 1106 2 0.7043735006759917 1106 3 0.4021841908931876 1106 4 0.5908139916329205 1106 5 0.8677797163985446 1106 6 0.6596763177088931 1106 7 0.46111270441902263 1106 8 0.231903848869635 1106 9 0.4503381331805443 1106 10 0.6212193558379369 1106 11 0.4343193894492856 1106 12 0.5023431854101474 1106 13 -0.40361849610917133 1106 14 -0.12051482031829508 1106 15 -0.16923513519328637 1106 16 -1.0426484763703954 1106 17 -0.33457270087565405 1106 18 -0.7366643787748757 1106 19 -0.4435201292080251 1106 20 -0.38755072011053215 1106 21 -0.44487127470377175 1106 22 -0.3691912136995441 1106 23 -0.6755387452817914 1106 24 0.26596153007017875 1106 25 -0.37179142194368353 1106 26 -0.7211549828296739 1106 27 -0.25465222343305527 1106 28 -0.09528663016981653 1106 29 -0.08496749754594646 1106 30 -0.26089331701486174 1106 31 0.051846329051826956 1106 32 0.32163050287989847 1106 33 -0.32453528645683605 1106 34 -0.08740456442748723 1106 35 -0.3376262658155085 1106 36 -0.5468464749786719 1106 37 -0.7303590339436212 1106 38 -0.31345714235213507 1106 39 -0.35951779576600273 1106 40 -0.4220003285091565 1106 41 -0.37334821053189415 1106 42 -0.4769831472045212 1107 1 0.5954634672847376 1107 2 0.5068993330525728 1107 3 0.7282461885969695 1107 4 0.6185981477752875 1107 5 0.7793755832182967 1107 6 0.04796564032657358 1107 7 0.7419393404695427 1107 8 0.8656581542657307 1107 9 0.5150912904710196 1107 10 0.4981424327610142 1107 11 0.660496197715652 1107 12 0.5622742990726053 1107 13 0.23564671629036837 1107 14 0.1821258226208542 1107 15 0.29000711509373645 1107 16 0.9231379759832237 1107 17 0.4116959558407629 1107 18 0.5579395247842527 1107 19 0.40056712681953144 1107 20 0.2863872821856859 1107 21 0.4286074853421553 1107 22 -0.04037376249402769 1107 23 0.5054027013312989 1107 24 0.4156745036637517 1107 25 0.558862998262974 1107 26 0.5075476578132694 1107 27 0.6314556984957633 1107 28 0.4243459760299544 1107 29 0.5689934668627785 1107 30 0.6653983018140721 1107 31 0.5058072934605491 1107 32 0.2584846934654337 1107 33 0.4557172967808256 1107 34 0.4611603035403675 1107 35 0.533556282978978 1107 36 0.37600082697311166 1107 37 0.30431375595305354 1107 38 0.5138677715399425 1107 39 0.4868656715129873 1107 40 0.33620862671472196 1107 41 -0.06335969158814692 1107 42 0.12209836829490683 1108 1 -0.1797779725747901 1108 2 -0.5139522911792085 1108 3 0.44290817279363637 1108 4 0.22746557534332368 1108 5 -0.15258227455168338 1108 6 0.6820745077541446 1108 7 0.015863835640743673 1108 8 0.10973190316828152 1108 9 0.5019218435877875 1108 10 -0.016563449591927792 1108 11 -0.09169871009822117 1108 12 0.3923884342789279 1108 13 0.08529553104014553 1108 14 -0.3055826936214636 1108 15 0.09773319059856789 1108 16 0.2082565010051738 1108 17 0.6507214167714039 1108 18 0.029625213985305265 1108 19 -0.7254205816967172 1108 20 0.10113706269490308 1108 21 0.1843142456582214 1108 22 -8.654218443382433E-4 1108 23 -0.011059107272743063 1108 24 0.31075791016068166 1108 25 -0.13604481560884096 1108 26 0.3080305375323178 1108 27 -0.01121330940590326 1108 28 0.1947586186989625 1108 29 0.028607163087535525 1108 30 -0.21541820389268906 1108 31 -0.37281430443233887 1108 32 -0.23493058309294668 1108 33 -0.11254433623059157 1108 34 -0.29871678162205484 1108 35 -0.32948146943542156 1108 36 0.32442049334711953 1108 37 0.10765001583013856 1108 38 0.0048686495030665355 1108 39 0.023740122786037432 1108 40 -0.1396473873326869 1108 41 -0.13579164944592037 1108 42 0.26871821026154663 1109 1 0.6255244300764176 1109 2 0.5256086036012348 1109 3 0.45202167735038756 1109 4 0.4018649125429974 1109 5 0.6315933675361407 1109 6 0.6744108789223304 1109 7 0.6504951529564025 1109 8 0.7584477275261889 1109 9 0.21111819818596445 1109 10 0.3432128552962226 1109 11 0.4267354241188457 1109 12 0.3058101409897773 1109 13 0.23711823195149861 1109 14 0.14470728152352308 1109 15 0.5815774325206888 1109 16 0.7478173957856171 1109 17 0.017539975180682177 1109 18 0.6043973763713879 1109 19 0.35452970827843444 1109 20 0.647118835685788 1109 21 0.8166671868346924 1109 22 0.5757708664738104 1109 23 0.5550138007637102 1109 24 0.760219384891414 1109 25 0.9811879930075591 1109 26 0.5882708026524526 1109 27 0.8172871048442971 1109 28 0.7560676493534757 1109 29 0.8790628383153773 1109 30 0.44593225178253704 1109 31 0.5341865240680761 1109 32 0.3963867325657091 1109 33 0.40547554777935346 1109 34 0.4514903434815025 1109 35 0.3962848934477634 1109 36 0.6431860277298895 1109 37 0.5790667515385053 1109 38 0.5357302899338876 1109 39 0.4450325834322655 1109 40 0.47095741796971424 1109 41 0.7014080191539165 1109 42 0.45844480512484154 1110 1 0.35791879627359435 1110 2 0.5115240965589862 1110 3 0.7477963252377151 1110 4 0.782146602683838 1110 5 0.6456778745783964 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22 -0.1646174091257393 2211 23 -0.11400028374333004 2211 24 -0.15108628697763749 2211 25 -0.11378726393952832 2211 26 0.08903212735743438 2211 27 0.051584782803949736 2211 28 -0.12002675490994363 2211 29 -0.1367346493130981 2211 30 0.13474077861923206 2211 31 -0.172893795688303 2211 32 -0.30726762601823054 2211 33 -0.5779656398871893 2211 34 -0.6023733793963046 2211 35 -0.3151730433622859 2211 36 -0.6449753731075702 2211 37 -0.6499016534862392 2211 38 -0.7332076621026573 2211 39 -0.20837434462254834 2211 40 -0.2787570852659158 2211 41 -0.6103544475381284 2211 42 -0.3942388644602346 2212 1 -0.25253339053143975 2212 2 -0.424169009445464 2212 3 0.035787429759579936 2212 4 -0.2678904618083777 2212 5 -0.014811376718860458 2212 6 -0.4905261114409001 2212 7 -0.6969844615728817 2212 8 -0.524169025624289 2212 9 -0.5646416239044747 2212 10 -0.1729101988179309 2212 11 -0.6598101652065829 2212 12 -0.35838555952912543 2212 13 0.2617661192754426 2212 14 0.22847303393271545 2212 15 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2215 31 0.28895040145001616 2215 32 -0.14081293603412348 2215 33 0.002161546122351865 2215 34 -0.23401089926911567 2215 35 -0.4588932341412999 2215 36 -0.3314618595940573 2215 37 0.1429441334771937 2215 38 0.1254568847971882 2215 39 -0.3821422301551394 2215 40 0.1662349656084885 2215 41 -0.05932106121062475 2215 42 -0.1430464956208084 2216 1 -0.21186353407211556 2216 2 -0.3087383874358949 2216 3 -0.5270116133026193 2216 4 -0.3875076866887568 2216 5 -0.4233041996853686 2216 6 0.6787322617648357 2216 7 0.10744137574769752 2216 8 -0.196417829452038 2216 9 -0.5281583703159569 2216 10 -0.1950393854208059 2216 11 -0.7955757154458176 2216 12 -0.296782688715723 2216 13 0.21631157382089583 2216 14 0.586128536325063 2216 15 0.41992570396755013 2216 16 0.6748340411121276 2216 17 0.23360911730616607 2216 18 0.4393845722740721 2216 19 0.7084029477150509 2216 20 0.689372356812548 2216 21 0.7598490050165125 2216 22 0.725738856230528 2216 23 0.6700906253475765 2216 24 0.5881643272729775 2216 25 0.430131654979391 2216 26 0.13356529689060892 2216 27 0.38450861572778194 2216 28 0.46013295024976486 2216 29 0.4249975374190882 2216 30 0.5085122773907358 2216 31 0.38453863674413213 2216 32 0.7062458874952853 2216 33 0.1661989792774321 2216 34 0.34633669431377 2216 35 0.14244366425442223 2216 36 0.4480835949513988 2216 37 0.6584521548675681 2216 38 0.5723820184870263 2216 39 0.3011465398983333 2216 40 0.5120905805817486 2216 41 0.5112671740834926 2216 42 0.6263385311171561 >>END_MODULE >>Per sequence quality scores pass #Quality Count 26 15.0 27 49.0 28 108.0 29 227.0 30 335.0 31 598.0 32 1009.0 33 1431.0 34 2303.0 35 4409.0 36 8888.0 37 12037.0 38 12792.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.8076740345241059 1.2081174634058052 0.8845953711454493 97.09961313092464 2 0.7918372887491233 1.07237392819167 97.51589330558133 0.6198954774778851 3 3.2736815909142325 93.30105653718242 0.920793647202552 2.5044682247007986 4 2.6854596049863124 0.8235107802990882 0.47962715775661185 96.01140245695798 5 91.34162895927602 6.090497737556562 1.332579185520362 1.2352941176470587 6 2.0791384810298412 89.70159046175425 2.4479084183615756 5.771362638854324 7 91.86896224067328 2.579127169068573 0.4004434288816995 5.15146716137644 8 2.0044795366620667 4.692201533901947 90.99341643854211 2.309902490893871 9 30.204303264779746 19.81492793954614 24.36254213896242 25.61822665671169 10 30.628959276018097 11.029411764705882 30.87330316742081 27.468325791855204 11 28.696517863203386 17.132384550987624 23.809308324094395 30.361789261714595 12 27.720084164800106 26.525487001968372 28.294757799950226 17.459671033281296 13 42.10943157132513 24.624366401158582 14.149619840695149 19.116582186821145 14 26.502262443438912 30.26018099547511 25.18552036199095 18.052036199095024 15 40.08235480440734 23.337632073123828 19.115817099934386 17.46419602253445 16 39.537567023370514 22.929345489921044 21.766475871586614 15.766611615121832 17 18.743919820818533 21.069659057487385 19.592316915906878 40.594104205787204 18 18.76711398764398 23.777410667813257 18.319038674783318 39.13643666975945 19 27.899452463912393 23.464862663468935 25.578080456129236 23.057604416489433 20 26.347820185063686 27.13739508155924 22.990430080767403 23.52435465260967 21 26.5944209407027 23.37051197936698 22.320761973711 27.714305106219317 22 30.19162462387729 24.255107350512432 23.203094952602882 22.350173073007397 23 25.08088052306509 28.92468496187869 20.673740413112824 25.320694101943396 24 24.38406370896586 24.85237890545463 28.813827741453814 21.9497296441257 25 26.32293387027443 24.671387525169113 26.881744756905952 22.123933847650505 26 25.20757448926495 25.61706748716092 27.639646161851545 21.535711861722586 27 26.755050790706093 23.639736657541686 28.687133775253955 20.91807877649827 28 26.006198954774778 28.14868441890455 23.535666613877517 22.309450012443158 29 24.757358430804732 26.825184950566726 26.38175606886722 22.035700549761316 30 25.637429017443043 25.813895613221423 27.096672020995 21.452003348340533 31 22.38914027149321 26.873303167420815 25.99095022624434 24.74660633484163 32 23.865975882898578 27.52878894142666 24.990384832922334 23.614850342752426 33 25.236985588561346 25.949639148435555 26.338770616049413 22.47460464695369 34 27.07857288296645 25.042419854754417 25.440600891382548 22.438406370896587 35 23.798104115291512 25.259609511097036 28.678084206239678 22.26420216737178 36 24.32524151037307 25.57634442659668 25.895341734349902 24.203072328680346 37 24.621614895590596 26.44962783647429 26.241487749145946 22.68726951878917 38 24.035655301916243 26.307097124499446 26.456415013234995 23.200832560349312 39 25.070702957080478 26.272087603791945 28.772144166157602 19.885065272969975 40 25.153846153846153 25.407239819004523 28.857466063348415 20.581447963800905 41 25.14648989841859 27.786701658333524 27.17133096536277 19.895477477885116 42 25.545826828661284 24.566166655354195 26.118237969184822 23.769768546799703 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.5 9 2.0 10 2.0 11 22.0 12 42.0 13 44.5 14 47.0 15 55.5 16 64.0 17 64.0 18 94.5 19 125.0 20 170.5 21 216.0 22 216.0 23 234.5 24 253.0 25 301.5 26 350.0 27 437.5 28 525.0 29 525.0 30 549.5 31 574.0 32 738.5 33 903.0 34 903.0 35 1092.5 36 1282.0 37 1639.0 38 1996.0 39 2279.0 40 2562.0 41 2562.0 42 2804.0 43 3046.0 44 3223.5 45 3401.0 46 3378.0 47 3355.0 48 3355.0 49 3925.5 50 4496.0 51 4126.5 52 3757.0 53 3757.0 54 3911.0 55 4065.0 56 3746.5 57 3428.0 58 3001.0 59 2574.0 60 2574.0 61 2438.0 62 2302.0 63 1762.5 64 1223.0 65 1436.0 66 1649.0 67 1649.0 68 1106.0 69 563.0 70 472.0 71 381.0 72 381.0 73 326.5 74 272.0 75 339.0 76 406.0 77 290.0 78 174.0 79 174.0 80 137.5 81 101.0 82 72.0 83 43.0 84 43.0 85 33.0 86 23.0 87 11.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.002262392253568924 6 0.0 7 0.0 8 0.0 9 0.004524784507137848 10 0.002262392253568924 11 0.009049569014275696 12 0.004524784507137848 13 0.020361530282120316 14 0.002262392253568924 15 0.004524784507137848 16 0.0 17 0.0 18 0.027148707042827087 19 0.006787176760706772 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.002262392253568924 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.004524784507137848 40 0.002262392253568924 41 0.0 42 0.004524784507137848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 44201.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.15538109997511 #Duplication Level Percentage of deduplicated Percentage of total 1 54.6190107647928 17.562951064455557 2 14.02940969534933 9.022420307232867 3 8.858087666221065 8.545055541729825 4 6.177443185815802 7.9455215945340605 5 4.756209104341096 7.646885817062962 6 3.032435094631675 5.850546367729237 7 2.3218180538943223 5.226126105744214 8 1.2875536480686696 3.312142259224905 9 0.9427988461267853 2.728445057804122 >10 3.672694012523746 19.59005452365331 >50 0.16182368254414972 3.59494129092102 >100 0.13368043340603672 7.723807153684306 >500 0.007035812284528248 1.2511029162236147 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTACGAGAATCAATCCTCGGCGCGGTGATGCGGCTCGCGGGC 553 1.2511029162236147 No Hit CTACGCGTTGCAGGATTCTCGAAGAGAAAACACGTGATTTTC 346 0.7827877197348476 No Hit CTACGAGTATCTTAGGTTTGGTCTTCTCATTTTGTCCTGTAT 332 0.7511142281848826 No Hit CTACGAGTCTGTGAAGCGGAGCGTTCCGTTCCCGGCTTCAGG 260 0.5882219859279202 No Hit CTACGAGTGGGTATGGCAGCAGCTGTGACGATGGACTGTCAT 238 0.5384493563494038 No Hit CTCCGAGGAGCTGGGAAATGGGTGTTTGTTCCATCGTGGGAG 195 0.44116648944594017 No Hit CTACAAGTGGGAGCAGCTGGATCATTTCATCATTAGACAAAA 195 0.44116648944594017 No Hit CTACGAACCTGACCGGCGGCGACCGCCGGCAGTTCGTGGTTT 194 0.4389040971923712 No Hit CTACGAGTACATAGCCCCATCCCTGTCCATGGAAAAACTGTC 184 0.41628017465668193 No Hit CTAGAAGTATCATATTCTCGGGACGAAGGCTGCATATATAAC 180 0.40723060564240626 No Hit CTACGAGTCTGCAGCAAACAGGATTAGATACCCTGGTAGTCC 176 0.39818103662813065 No Hit CTACGAGTCGGCGTGGGCTGCCCCTATAGGCAACTTCTTTGC 156 0.3529331915567521 No Hit CTACGAGTGGATGAGGCCCCTTGGTATTGCAAACTTTATATG 140 0.3167349154996493 No Hit CTGCGTGTTCAGGCTGGAGTTAAAATGGTTATGATTCAAGAC 136 0.3076853464853736 No Hit CTACGAGTTCGAGGCTGAGAGAAGATGTGATGATCCAGATAA 123 0.27827424718897764 No Hit CTACGACTGGTAAATAGGACCGCGGTTCTATTTTGTTGGTTT 120 0.27148707042827086 No Hit CTACGACCCTCGGAAGTGTTGGTGGTTACAGTTATACATCGG 116 0.2624375014139952 No Hit CTACGCGTTGCAGGGATTCTCGAAGAGAAAACACGTGATTTT 111 0.25112554014615057 No Hit CTGCGGGTCTGGCTACGTCGGCGCCGAAGGCCGCCGGCGTAG 108 0.24433836338544376 No Hit CTACGAGTGCGGGAAGGCAGATGTGTTTTTCATTCTGTTTTT 104 0.23528879437116806 No Hit CTACGAGTCCACGAATCACCCAGAACCGCTAAGCCACCAGAA 99 0.22397683310332347 No Hit CTACGAGTGAGACAGTCCGACGATCCGGAACGATGTCTTTAA 92 0.20814008732834097 No Hit CTACGAGTTGTCGGAGCCTTGGGGTGACTTCATGCCTCTTTT 91 0.20587769507477205 No Hit CTACGAGTTCGAGCTGAGAGAAGATGTGATGATCCAGATAAC 86 0.19456573380692746 No Hit CTACGAGTCTTTGCCGCCGAAGGAGGGGCAAAGCTCAAGATG 81 0.18325377253908284 No Hit CTACGAGTTCCGGTGGCGCACGCCGGTAGGATTTGCTGAAGG 80 0.1809913802855139 No Hit CTACGAGTACTTGAGACAGCAGGACTGTCCCCTCTAGGAGGT 71 0.16062985000339358 No Hit CTACGAGAATCAGATCCTCGGCGCGGTGATGCGGCTCGCGGG 69 0.15610506549625572 No Hit CTACGAGTCCCATTCTCTTTCAATTGATTCCTTACCTCTCTC 68 0.1538426732426868 No Hit CTACGAGTGCTAAAGGCTGTATTTTACTCAGAAAATAATCTT 68 0.1538426732426868 No Hit CTACGAGTAGGTGGGGAAGTGGCGAGGTCCACGCCCGGCTTG 66 0.14931788873554896 No Hit CTACGAGTGTCCGGTGTCTGTGTCTGTCATTCCTAAAGTGCA 66 0.14931788873554896 No Hit CTACGAGTTGTTGCAGAATTTGAATTCAAACGAATATTTTAT 65 0.14705549648198005 No Hit CTACGAGTATACGCCGGCGCCTAGCAGCCGACTTAGAACTGG 64 0.14479310422841113 No Hit CTACGAGAATCACATCCTCGGCGCGGTGATGCGGCTCGCGGG 63 0.14253071197484218 No Hit CTGCGTGTTCAGGGCTGGAGTTAAAATGGTTATGATTCAAGA 63 0.14253071197484218 No Hit CTACGAGAATCATATCCTCGGCGCGGTGATGCGGCTCGCGGG 63 0.14253071197484218 No Hit CTACGAGTACGTGGGGCTGTGGCTGTTTCTGCTGCCCCTGGC 59 0.1334811429605665 No Hit CTACGAGTCTGTGGAAGCGGAGCGTTCCGTTCCCGGCTTCAG 58 0.1312187507069976 No Hit CTACGAGTCTCTGACGCCAGAGCCGAGCCCGCGTCGCCATGC 56 0.12669396619985973 No Hit CTACGAGTCTGAAGCTGGTATTTGATATCTTTACTTGAAGGG 55 0.12443157394629081 No Hit CTACGACTCGTGAATTATTCCCCATGAACGAGGAATTCCCAG 53 0.11990678943915296 No Hit CTACGAGTACAATCGGAACAAGAAGAGGGTAAGGTGGCTCAG 53 0.11990678943915296 No Hit CTACGAGTCCTGATGGTACAGGTTGGGCGTTGTGGCTCATGC 50 0.1131196126784462 No Hit CTACGAGTTCTGGGAGCCATGGAGTTGCTCAAAGTGTCCAAG 49 0.11085722042487725 No Hit CTACGAGTAGCCGTGGCGCACGCCTTTAATCCCAGCACTTGG 49 0.11085722042487725 No Hit CTACGAGTACGGGGGCCCCAGAGCACTGGCATGGTGTGTGTG 49 0.11085722042487725 No Hit CTACGAGTACGTGAAAGAAAACAGCTTTGAAATGCAAAAAGT 49 0.11085722042487725 No Hit CTACGAGTGAATGAAGCCTGAGGTGGTGAAGACGCACCTGAG 47 0.10633243591773943 No Hit CTACGAGTACAACGGAACAAGAAGAGGGTAAGGTGGCTCAGC 46 0.10407004366417048 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.004524784507137848 0.0 0.0 12 0.0 0.0 0.022623922535689237 0.0 0.0 13 0.0 0.0 0.03393588380353386 0.0 0.0 14 0.0 0.0 0.040723060564240625 0.0 0.0 15 0.0 0.0 0.05203502183208525 0.0 0.0 16 0.0 0.0 0.05655980633922309 0.0 0.0 17 0.0 0.0 0.06787176760706772 0.0 0.0 18 0.0 0.0 0.09275808239632588 0.0 0.0 19 0.0 0.0 0.14705549648198005 0.0 0.0 20 0.0 0.0 0.17646659577837606 0.0 0.0 21 0.0 0.0 0.19230334155335851 0.0 0.0 22 0.0 0.0 0.8687586253704668 0.0 0.0 23 0.0 0.0 0.9750910612882061 0.0 0.0 24 0.0 0.0 1.0293884753738602 0.0 0.0 25 0.0 0.0 1.2058550711522364 0.0 0.0 26 0.0 0.0 1.2375285627022012 0.0 0.0 27 0.0 0.0 1.425307119748422 0.0 0.0 28 0.0 0.0 1.4931788873554896 0.0 0.0 29 0.0 0.0 1.5497386936947128 0.0 0.0 30 0.0 0.0 1.6696454831338658 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTGAAG 30 9.760475E-6 36.028313 10 ACTCGTG 40 4.6675268E-8 36.028313 6 ACCGGCG 35 6.7370456E-7 36.028313 12 GGGTCTG 30 9.760475E-6 36.028313 5 GTATGAG 25 1.4203685E-4 36.028313 7 ACTCGCT 25 1.4203685E-4 36.028313 6 ATCTTAG 25 1.4203685E-4 36.028313 9 TAGTGCT 20 0.002077254 36.028313 5 TATACGC 20 0.002077254 36.028313 8 GAGTGTA 40 4.6675268E-8 36.028313 5 GAGTGGG 130 0.0 36.028313 5 TGACCGG 35 6.7370456E-7 36.028313 10 GAGTGCG 135 0.0 36.028313 5 ACCACTT 30 9.760475E-6 36.028313 3 GGGTATG 45 3.243258E-9 36.028313 9 CCAGTCC 20 0.002077254 36.028313 12 CCAGTAT 20 0.002077254 36.028313 4 AGTAGCT 45 3.243258E-9 36.028313 6 CAGTATT 20 0.002077254 36.028313 5 GTTCTTG 25 1.4203685E-4 36.028313 7 >>END_MODULE