FastQCFastQC Report
Thu 26 May 2016
SRR2099172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176171
Sequences flagged as poor quality0
Sequence length42
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAAGGCCAGGGGAAGACGTGCATGTACGGATCGTCCTCGC34491.9577569520522673No Hit
ACGATGCGTTGTGTGAACACCACTGACTCCGCCGTGCGTATT19781.1227727605565048No Hit
ACGTGGTCCACCGGAAGAAAAGCTCGGCAAGATCTTCGAACG16170.9178582173002368No Hit
ACGATCTCCGCTGCGCGCTCCAGCGTCTGCTGGCGCATGTTG14210.806602675809299No Hit
ACGATGTCGGCCTTGAGAGCAATCAGCTCCAAAGTTCATCCC11160.6334754301218702No Hit
ACGATGTCTCGACCTGTCTCAGTTATTGAAGTAGAACCAAAT11010.6249609754159311No Hit
ACGATGCCGTGCTCGCCACGTTCGTCGCGCTGTTCGGCGTGG10220.5801181806313185No Hit
ACGATGTCGATCCTTAATCACCTCCTCTAAAGGTTTTTAAGT8510.4830533969836125No Hit
ACATTGTCGACAGCGGCGCGGCCCCTTGTGGAAGTTGTCGGC8250.46829500882665137No Hit
ACGAAGGCCAGGGGGAAGACGTGCATGTACGGATCGTCCTCG7900.4484279478461268No Hit
ACGATGTCGGTCGACCGGCATTCCGTATATCGATCAGGGCTA7500.42572273529695576No Hit
ACGATCTCCGCTAGCGCGCTCCAGCGTCTGCTGGCGCATGTT6530.3706625948652162No Hit
GCGAGGTCGCTGCGCCATCGCCGCGCCGGCCGTCGCGCAGTC5980.3394429276101061No Hit
ACGATGACACTCGCAATCTTGCCGATTGCTTAACTAAAGTTT5740.32581980008060346No Hit
ACGATGTATTGCGCGAGGAACAGAGCATGAGGTGCTCACCCT5520.31333193317855945No Hit
ACGATGAACAGATTTCGACATCCTGGTGCCGCGCGAGGACGC5220.2963030237666812No Hit
ACGATGTCGGTCGGACCGGCATTCCGTATATCGATCAGGGCT5050.28665330843328357No Hit
ACGATGTCCCGCATTTGAAACCTAGCGGAGGGAGGAGGCAGG4840.2747330718449688No Hit
ACGACGTCGCGCGATGCCCGCGACCTGAAGTAGTGCGTGGCC4630.26281283525665405No Hit
ACGACGTCGCGCGAGGCCCGCGACCTGAAGTAGTGCGTGGCC4540.2577041624330906No Hit
ACGGCGTCGCACGCATCAAGCTGCCCGAAGGCGTAACGGTGC4500.2554336411781735No Hit
ACGATGCGTTGTGGTGAACACCACTGACTCCGCCGTGCGTAT4220.2395399923937538No Hit
ACGATGTCTATCTGTGTTCTACCTTCATTTCTCAAGCAAGAC4010.22761975580543903No Hit
ACGATGTATGGCCACGTGGATGCTGCCCTCGGTCGGCGTCGC3950.2242139739230634No Hit
ACGGCGTCGCACAGCATCAAGCTGCCCGAAGGCGTAACGGTG3820.21683477984458283No Hit
ACGATGCCGTGCGTCGCCACGTTCGTCGCGCTGTTCGGCGTG3660.20775269482491443No Hit
ACGATGCCGTGCTTCGCCACGTTCGTCGCGCTGTTCGGCGTG3650.20718506451118515No Hit
ACGATGTCTATAAAAGAGAGAAAGAGGAAGGGAAGAAGAAAA3450.19583245823659967No Hit
ATGATATCAGCTGGTAAATTTGCCTGATTTCTTACAGGCTAC3360.1907237854130362No Hit
ACGATCTCCATGGCTGCACCATGAGGCTGTTGTGATAGATCA3340.18958852478557764No Hit
ACGGTGCCAGAGGCTTGGTGAGATTGGCTAAGCAATTTGGAA3310.18788563384438983No Hit
ACGATGTCTCGCGTCGCGAGACCCTGGCCTGGCTGGACGGCG3190.18107407007963852No Hit
ACATTGTCGACAGGCGGCGCGGCCCCTTGTGGAAGTTGTCGG3180.18050643976590927No Hit
ACGATGCCATACTTTGTCTTCGTCCACGGCTATAACAACAAT3170.17993880945217997No Hit
ACGATGTCTTCTCTGGGAAAGTTTGGGGAAGCTGGGTGTCTT3130.1776682881972629No Hit
AAGATATCTCAGCCGACGTCTCAGTTACATTACCATGGATCT3110.17653302756980435No Hit
ACGATCGCTGGGGCCACGTCGGCGCCACTAGGTATCGTCCGC3060.17369487600115796No Hit
ACGATGCCGTGCCTCGCCACGTTCGTCGCGCTGTTCGGCGTG3040.1725596153736994No Hit
ACGATGTCACATCAGTCCGACGATCTTGTACGTGATCGTCGG3040.1725596153736994No Hit
ACGGCGTCGCACCATCAAGCTGCCCGAAGGCGTAACGGTGCA2990.16972146380505304No Hit
ACGAAGGCCAGGTGGAAGACGTGCATGTACGGATCGTCCTCG2710.15382781502063336No Hit
ACGGTGCCAGAGGGCTTGGTGAGATTGGCTAAGCAATTTGGA2700.15326018470690408No Hit
ACGATATCTATACCATCAAGCAGACTGATCCGGTTCTTGCCA2600.14758388156961136No Hit
ACGATGCCACCGGCTGATAGCAGTGCTTTCCCCCATGTGGCC2550.14474573000096497No Hit
ACGACGTCGCGCGAGGCCCGCGACCTGAAGGAGTGCGTGGCC2500.1419075784323186No Hit
ACGATGTCCTGCGTGGACTCAAGCAGTCTCTCAACCCACAGG2450.13906942686367224No Hit
ACGATCTCCGCTGGCGCGCTCCAGCGTCTGCTGGCGCATGTT2320.13169023278519165No Hit
ACGTGGTCCACCGGGAAGAAAAGCTCGGCAAGATCTTCGAAC2290.12998734184400385No Hit
ACGATGTCCCGCGTCCGACGATCGGGCACGATGTCGGACTGT2270.1288520812165453No Hit
ACGATGTCTTGAGAAGATCAAAGAGAAATACATTGATCAGGA2200.12487866902044036No Hit
ACGAAGGCCAGGAGGAAGACGTGCATGTACGGATCGTCCTCG2180.12374340839298181No Hit
ACGATGTCGCGGAGAAAAGATGGAGAAGTGGGTGGTCCCTTC2120.12033762651060619No Hit
ACGACGTCGCGCGATGCCCGCGACCTGAAGGAGTGCGTGGCC2050.11636421431450124No Hit
ACGTAGTCTCGATCGAACGGAGCTTCAGTGACTACGTTCGAG1990.11295843243212561No Hit
AAGATATCAGGCCAGTCCGACGATCGCTAACGATATCTTGCC1970.11182317180466707No Hit
ATGATGTGTCGAGGGCGAAGGAAGCTGCAGAGCAAGATGTTG1880.10671449898110358No Hit
ACGATGTCTTCTACTGGGAAAGTTTGGGGAAGCTGGGTGTCT1810.10274108678499866No Hit
ACGATGTCCAGACAGACTTTAGTGTTTTAACCTGATACCTAT1810.10274108678499866No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG200.002050960236.18161436
CTTACTG200.002050960236.18161436
TCATTGG200.002085704636.0580567
CGTGAGT404.7706635E-836.0580568
TATGGCC502.3464963E-1036.0580567
CGCATAC200.002085704636.0580568
TTGTACT251.4307768E-436.0580569
CCTCGTG200.002085704636.0580568
TCTAGTC251.4307768E-436.0580567
CTGCGTG251.4307768E-436.0580569
TCGGATA251.4307768E-436.0580567
TATCTGT404.7706635E-836.0580569
CGCTATG200.002085704636.0580568
CGCTATC200.002085704636.0580568
CGCTAAA251.4307768E-436.0580568
TGTTCGC200.002085704636.0580568
CCATACT800.036.0580567
TCATGCA200.002085704636.0580567
CATACGT200.002085704636.0580568
CATACGG251.4307768E-436.0580568
Produced by FastQC (version 0.11.5)