FastQCFastQC Report
Thu 26 May 2016
SRR2099171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53121
Sequences flagged as poor quality0
Sequence length42
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAAGGCCAGGGGAAGACGTGCATGTACGGATCGTCCTCGC10361.9502644905028141No Hit
ACGATGCGTTGTGTGAACACCACTGACTCCGCCGTGCGTATT5921.1144368517158938No Hit
ACGTGGTCCACCGGAAGAAAAGCTCGGCAAGATCTTCGAACG4740.8923024792454961No Hit
ACGATCTCCGCTGCGCGCTCCAGCGTCTGCTGGCGCATGTTG3640.6852280642307186No Hit
ACGATGTCGGCCTTGAGAGCAATCAGCTCCAAAGTTCATCCC2950.5553359311759944No Hit
ACGATGTCTCGACCTGTCTCAGTTATTGAAGTAGAACCAAAT2890.5440409630842793No Hit
ACGATGCCGTGCTCGCCACGTTCGTCGCGCTGTTCGGCGTGG2770.521451026900849No Hit
ACGATGTCCTGCGTGGACTCAAGCAGTCTCTCAACCCACAGG2450.4612111970783683No Hit
ACGATGTCGATCCTTAATCACCTCCTCTAAAGGTTTTTAAGT2360.4442687449407955No Hit
ACGATGTCGGTCGACCGGCATTCCGTATATCGATCAGGGCTA2310.43485627153103296No Hit
ACGAAGGCCAGGGGGAAGACGTGCATGTACGGATCGTCCTCG2300.4329737768490804No Hit
ACATTGTCGACAGCGGCGCGGCCCCTTGTGGAAGTTGTCGGC2300.4329737768490804No Hit
ACGATCTCCGCTAGCGCGCTCCAGCGTCTGCTGGCGCATGTT1750.3294365693416916No Hit
GCGAGGTCGCTGCGCCATCGCCGCGCCGGCCGTCGCGCAGTC1750.3294365693416916No Hit
ACGATGTATTGCGCGAGGAACAGAGCATGAGGTGCTCACCCT1720.32378908529583406No Hit
ACGATGTCCCGCATTTGAAACCTAGCGGAGGGAGGAGGCAGG1650.3106116225221664No Hit
ACGATGTCGGTCGGACCGGCATTCCGTATATCGATCAGGGCT1580.2974341597484987No Hit
ACGACGTCGCGCGAGGCCCGCGACCTGAAGTAGTGCGTGGCC1500.2823742022928785No Hit
ACGATGACACTCGCAATCTTGCCGATTGCTTAACTAAAGTTT1460.27484422356506844No Hit
ACGACGTCGCGCGATGCCCGCGACCTGAAGTAGTGCGTGGCC1420.26731424483725835No Hit
ACGATGAACAGATTTCGACATCCTGGTGCCGCGCGAGGACGC1390.2616667607914008No Hit
GCTATGTCCGTTTAGCAACGGATCGTCGGACTGTAGAACTCT1300.24472430865382805Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACGATCGCTGGGGCCACGTCGGCGCCACTAGGTATCGTCCGC1270.2390768246079705No Hit
ACGATGCGTTGTGGTGAACACCACTGACTCCGCCGTGCGTAT1240.2334293405621129No Hit
ACGGCGTCGCACGCATCAAGCTGCCCGAAGGCGTAACGGTGC1180.22213437247039775No Hit
ACGATGTCTATCTGTGTTCTACCTTCATTTCTCAAGCAAGAC1140.21460439374258766No Hit
ACGATGTATGGCCACGTGGATGCTGCCCTCGGTCGGCGTCGC1040.19577944692306246No Hit
ACGATGCCGTGCTTCGCCACGTTCGTCGCGCTGTTCGGCGTG1020.1920144575591574No Hit
ACGATGTCTTCTCTGGGAAAGTTTGGGGAAGCTGGGTGTCTT1010.19013196287720488No Hit
ACGATGTCGTGAGTGACCATGAACCTTTAGTCTTCAGCCATG970.18260198414939477No Hit
ACGGCGTCGCACAGCATCAAGCTGCCCGAAGGCGTAACGGTG970.18260198414939477No Hit
ACGATGCCATACTTTGTCTTCGTCCACGGCTATAACAACAAT950.17883699478548973No Hit
ACGATCTCCATGGCTGCACCATGAGGCTGTTGTGATAGATCA940.1769545001035372No Hit
ACGATGCCGTGCGTCGCCACGTTCGTCGCGCTGTTCGGCGTG870.16377703732986956No Hit
ACGGTGCCAGAGGCTTGGTGAGATTGGCTAAGCAATTTGGAA870.16377703732986956No Hit
AAGATATCTCAGCCGACGTCTCAGTTACATTACCATGGATCT870.16377703732986956No Hit
ACGAAGGCCAGGTGGAAGACGTGCATGTACGGATCGTCCTCG850.1600120479659645No Hit
ACGATGCCGTGCCTCGCCACGTTCGTCGCGCTGTTCGGCGTG850.1600120479659645No Hit
ACGGCGTCGCACCATCAAGCTGCCCGAAGGCGTAACGGTGCA840.158129553284012No Hit
ACGACGTCGCGCGATGCCCGCGACCTGAAGGAGTGCGTGGCC840.158129553284012No Hit
ACGGTGCCAGAGGGCTTGGTGAGATTGGCTAAGCAATTTGGA830.15624705860205945No Hit
ACGATGTCTCGCGTCGCGAGACCCTGGCCTGGCTGGACGGCG810.1524820692381544No Hit
ACATTGTCGACAGGCGGCGCGGCCCCTTGTGGAAGTTGTCGG810.1524820692381544No Hit
ACGACGTCGCGCGAGGCCCGCGACCTGAAGGAGTGCGTGGCC800.15059957455620188No Hit
ACGATGTCACATCAGTCCGACGATCTTGTACGTGATCGTCGG770.1449520905103443No Hit
ACGATATCTATACCATCAAGCAGACTGATCCGGTTCTTGCCA760.14306959582839177No Hit
ACGATGCCACCGGCTGATAGCAGTGCTTTCCCCCATGTGGCC760.14306959582839177No Hit
ACGAAGGCCAGGAGGAAGACGTGCATGTACGGATCGTCCTCG740.13930460646448672No Hit
ACTAGGTCGCTCAGTGAGCGATCGTCGGACTGTAGAACTCTG740.13930460646448672Illumina DpnII expression Sequencing Primer (100% over 23bp)
AAGATATCTCTCGAGTTCTCCAGTCCGACGATCGCCTACGAT740.13930460646448672Illumina Small RNA Adapter 1 (95% over 21bp)
ACGATGTCTTGAGAAGATCAAAGAGAAATACATTGATCAGGA710.13365712241862918No Hit
ACGATGTCTCCGATCGCCAACGATGTCGGACTGTAGAACTCT670.1261271436908191Illumina NlaIII expression Adapter 1 (95% over 21bp)
ACGATGTCGGGTGTGGCGGCGACGACCCATTCGAACGTCTGC670.1261271436908191No Hit
ACGATGTCGCGGAGAAAAGATGGAGAAGTGGGTGGTCCCTTC670.1261271436908191No Hit
ACGATGTCCCGGGTGGCGCTCTGAGTGTATCGTCCTTCTTCG650.12236215432691402No Hit
ACGATGTCTCCAATGGCTCAGCGGTTAAAAGCATGAACTGTT630.11859716496300898No Hit
ATGATATCAGCTGGTAAATTTGCCTGATTTCTTACAGGCTAC630.11859716496300898No Hit
ACGATGTCGTGCGTGGAATGTCATGAAATAGACCAATATACA620.11671467028105645No Hit
ACGATGTCCCTTGTTGACTAGGCGCATAGTCGCCATTCACAG620.11671467028105645No Hit
ACGATCTCCGCTGGCGCGCTCCAGCGTCTGCTGGCGCATGTT610.11483217559910394No Hit
ACGTAGTCTCGATCGAACGGAGCTTCAGTGACTACGTTCGAG590.11106718623519887No Hit
ACGATGTCTCCTGAGGCACACAGCCAGCACAGTGAAGAGGCA580.10918469155324637No Hit
GCTATGTCAGCGTAGCCGCTGATCGTCGGACTGTAGAACTCT580.10918469155324637Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACGATGTCTAACGGGTCCTCCAAGCCCTGGAGGGGCTGAAAA560.10541970218934131No Hit
AAGATATCAGGCCAGTCCGACGATCGCTAACGATATCTTGCC560.10541970218934131No Hit
ACGATGTCTTCTACTGGGAAAGTTTGGGGAAGCTGGGTGTCT550.1035372075073888No Hit
ACGATGTCGGCCGTTGAGAGCAATCAGCTCCAAAGTTCATCC540.10165471282543627No Hit
ACGTGGTCCACCGGGAAGAAAAGCTCGGCAAGATCTTCGAAC540.10165471282543627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAC200.002075413736.0481158
TAGCGCG200.002075413736.04811512
TCTAGTC200.002075413736.0481157
CTATACC356.7421934E-736.0481158
TATACCC200.002075413736.0481159
CTCCTGA200.002075413736.0481158
ACTTGCA200.002075413736.0481157
CCTCGGG200.002075413736.0481158
TCGCGCG404.6753485E-836.0481157
TCGGACC200.002075413736.04811511
GAGTTCT200.002075413736.04811513
CCCGCCT200.002075413736.0481158
TCGCTCA200.002075413736.0481157
TCTGACA200.002075413736.0481159
GCAGACC200.002075413736.04811512
TCTTGAG200.002075413736.0481157
TCGTCAG309.760786E-636.0481157
TCGCAAT200.002075413736.04811511
CTTGTTG200.002075413736.04811510
GATTTCG200.002075413736.04811511
Produced by FastQC (version 0.11.5)