##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099171_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 53121 Sequences flagged as poor quality 0 Sequence length 42 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.44978445435892 37.0 35.0 37.0 33.0 37.0 2 35.81077163457013 37.0 36.0 37.0 35.0 37.0 3 35.65170083394514 37.0 36.0 37.0 35.0 37.0 4 33.58131435778694 35.0 33.0 35.0 31.0 37.0 5 36.40893431976055 39.0 37.0 39.0 32.0 39.0 6 36.866342877581374 39.0 37.0 39.0 33.0 39.0 7 36.83925377910808 39.0 37.0 39.0 33.0 39.0 8 37.31665443045124 39.0 38.0 39.0 35.0 39.0 9 31.801566235575386 33.0 31.0 34.0 30.0 34.0 10 32.19478172474163 34.0 31.0 34.0 30.0 34.0 11 32.28516029442217 34.0 31.0 34.0 30.0 34.0 12 35.66261930310047 37.0 35.0 37.0 33.0 37.0 13 31.98015850605222 31.0 31.0 34.0 30.0 34.0 14 31.999698800850886 33.0 31.0 34.0 30.0 34.0 15 31.83746540916022 33.0 31.0 34.0 30.0 34.0 16 34.65569172267088 37.0 35.0 37.0 30.0 37.0 17 35.22966435119821 37.0 35.0 37.0 32.0 37.0 18 35.08427928691101 37.0 35.0 37.0 32.0 37.0 19 34.60296304662939 37.0 35.0 37.0 30.0 37.0 20 34.71243011238493 37.0 35.0 37.0 30.0 37.0 21 36.38726680597127 39.0 35.0 39.0 30.0 39.0 22 36.53340486813125 39.0 35.0 39.0 31.0 39.0 23 36.89514504621525 39.0 37.0 39.0 32.0 39.0 24 37.022156962406584 39.0 37.0 39.0 33.0 39.0 25 37.12614596863764 39.0 37.0 39.0 33.0 39.0 26 38.58699949172644 40.0 38.0 41.0 34.0 41.0 27 38.755219216505715 40.0 38.0 41.0 35.0 41.0 28 38.946819525234844 40.0 38.0 41.0 36.0 41.0 29 39.03580504885074 40.0 38.0 41.0 36.0 41.0 30 39.10705747256264 40.0 38.0 41.0 36.0 41.0 31 39.19521469851848 40.0 39.0 41.0 36.0 41.0 32 39.24711507689991 40.0 39.0 41.0 36.0 41.0 33 39.207639163419366 40.0 39.0 41.0 36.0 41.0 34 39.20955930799496 40.0 39.0 41.0 36.0 41.0 35 39.20025978426611 40.0 39.0 41.0 36.0 41.0 36 39.218802356883344 40.0 39.0 41.0 36.0 41.0 37 39.10743397149903 40.0 39.0 41.0 36.0 41.0 38 39.09041621957418 40.0 39.0 41.0 36.0 41.0 39 39.063101221739046 40.0 39.0 41.0 36.0 41.0 40 38.98896858116376 40.0 39.0 41.0 35.0 41.0 41 38.9778242126466 40.0 38.0 41.0 35.0 41.0 42 38.983226972383804 40.0 39.0 41.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5824735439719149 1101 2 0.5571243728930071 1101 3 0.6565826188281179 1101 4 0.49330645578077537 1101 5 0.5418216467895505 1101 6 0.47802862921103184 1101 7 0.5641871156098262 1101 8 0.5535951219040669 1101 9 0.6993931333216175 1101 10 0.36692007836585816 1101 11 0.7634444270930913 1101 12 0.6634471095269845 1101 13 0.31883433388760096 1101 14 0.3899366966483342 1101 15 0.09878229638792746 1101 16 0.8126269010945109 1101 17 -0.18148883327552312 1101 18 -0.1342436739456545 1101 19 -0.2073240449541771 1101 20 -0.8039731840003697 1101 21 0.10959805123981425 1101 22 -0.5004533173514645 1101 23 -0.32007869974206926 1101 24 -0.3974830283436148 1101 25 -0.2348222331570966 1101 26 -0.5401844735915162 1101 27 -0.22555192845470629 1101 28 0.3102614262764476 1101 29 0.43809929572894646 1101 30 0.026854509230858525 1101 31 0.31313115691439464 1101 32 0.45247563494955045 1101 33 0.235664392075293 1101 34 -0.11402565961103761 1101 35 0.07577810668161078 1101 36 0.10170049471282994 1101 37 0.03575531669249443 1101 38 0.1504225631334961 1101 39 -0.14782239877402503 1101 40 -0.3137960614672295 1101 41 -0.29892687116289096 1101 42 -0.3199799365101512 1102 1 0.8510423845516257 1102 2 0.687106256950976 1102 3 0.9181315318715946 1102 4 1.5933970354909235 1102 5 1.076921284470707 1102 6 0.7696952958776961 1102 7 0.8635530576388106 1102 8 0.7800443972663871 1102 9 0.728831539118719 1102 10 0.6416030493803504 1102 11 0.7700114560785991 1102 12 0.5916626892371326 1102 13 0.32404266722093666 1102 14 0.44858705896717765 1102 15 0.19026780363430262 1102 16 0.2836413938481357 1102 17 0.5687376159998365 1102 18 0.7966892970688377 1102 19 0.06871762171248719 1102 20 0.10590000440542724 1102 21 0.23505094979054064 1102 22 -0.2307522303949412 1102 23 -0.9761022504667096 1102 24 -0.31007360805376294 1102 25 0.28261436104580184 1102 26 0.023900671336022583 1102 27 0.3614045932844263 1102 28 0.8295096146822445 1102 29 0.7687152377579309 1102 30 0.5116824077815849 1102 31 0.3894445627114962 1102 32 0.38589954799302717 1102 33 0.4759270732347147 1102 34 0.2381029635773686 1102 35 0.4165842661018999 1102 36 0.6698617265968849 1102 37 0.7189527804606115 1102 38 0.3451689399450899 1102 39 0.6137265142694517 1102 40 0.8515119095472627 1102 41 0.6333647955037733 1102 42 0.07947658522898138 1103 1 0.21729497254334262 1103 2 -0.14912562710699007 1103 3 0.5231004759709776 1103 4 0.20312788435220597 1103 5 0.4404823610752615 1103 6 0.48874291492531796 1103 7 0.9097228298955429 1103 8 0.9214522647612071 1103 9 -0.08007115239266938 1103 10 0.15754507836585674 1103 11 0.09692656995023441 1103 12 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0.34546123996043576 1105 1 0.5024221741089008 1105 2 0.7399154687834155 1105 3 0.8139028243075686 1105 4 0.46633727769858524 1105 5 1.0604432221320153 1105 6 0.8838505470192501 1105 7 0.6570809512262628 1105 8 0.7196910123150246 1105 9 0.33851984565038507 1105 10 0.40138069480421734 1105 11 0.42119271476432374 1105 12 0.6837810136365761 1105 13 0.05920248457253052 1105 14 -0.041784193762623545 1105 15 -0.1674848268997451 1105 16 -0.22161967424795392 1105 17 -0.24206246341250903 1105 18 -0.2791494958634644 1105 19 -0.4883600038582898 1105 20 -0.9490930470140668 1105 21 -0.34545331862319983 1105 22 -0.2772084543377673 1105 23 -1.0197790422078228 1105 24 -0.36109604204224155 1105 25 -0.7654301098694276 1105 26 -0.761288925646312 1105 27 -0.45157932571498094 1105 28 -0.3898669983810876 1105 29 -0.23227741659982115 1105 30 -0.5208766551527049 1105 31 0.053927389791105895 1105 32 -0.3073702554614073 1105 33 0.17594692632187048 1105 34 -0.049171207556241825 1105 35 -0.1936996330444174 1105 36 0.12588713854844968 1105 37 -0.06762653262257601 1105 38 0.44066228916089045 1105 39 0.16703205328077075 1105 40 0.21402928099852403 1105 41 0.18587621102889074 1105 42 0.07471184431176425 1106 1 -0.4975778258910992 1106 2 0.041285331797119795 1106 3 0.10157405718427981 1106 4 0.0964742639999514 1106 5 -0.39161157238853406 1106 6 -0.5545056173643133 1106 7 -0.8634669939792161 1106 8 -0.38989802878086266 1106 9 -0.56558974339071 1106 10 -0.07807135999030379 1106 11 -0.20894427153704243 1106 12 -0.9463559726647972 1106 13 0.04550385443554461 1106 14 -0.34315405677632427 1106 15 -0.26337523785864647 1106 16 -0.207921044110968 1106 17 -0.8585008195768964 1106 18 -1.4298344273703094 1106 19 -1.3513737024884236 1106 20 -1.7299149648222851 1106 21 -1.6879190720478547 1106 22 -2.140222152967901 1106 23 -1.2115598641256327 1106 24 -0.6898631653299176 1106 25 -0.5051561372666882 1106 26 -0.3092341311257627 1106 27 -0.615962887358819 1106 28 -0.4583601490660172 1106 29 -0.13638700564091977 1106 30 0.12295896128565431 1106 31 0.04022875965411998 1106 32 -0.18408258422853407 1106 33 -0.3719982791575802 1106 34 -0.008075317145284089 1106 35 -0.0019188111266075225 1106 36 -0.24397587515018415 1106 37 0.041962508473318394 1106 38 -0.4908445601541729 1106 39 -0.18913233028087717 1106 40 -0.4709022258507929 1106 41 0.04888990965902451 1106 42 0.10210910458573608 1107 1 0.001544056792425863 1107 2 -0.2209204989018616 1107 3 -0.37506802219752444 1107 4 -0.37928969806537793 1107 5 -0.26867514808224513 1107 6 -0.33286880668640606 1107 7 -0.49430647413376505 1107 8 -0.4111484678395243 1107 9 -0.13794661026812705 1107 10 0.09087841169919386 1107 11 0.26945404247770455 1107 12 0.3369246736295466 1107 13 -0.15031630713804134 1107 14 0.0762347735714144 1107 15 0.08435921946485081 1107 16 -0.18737309890548914 1107 17 -0.4591330640447566 1107 18 0.3228877363107543 1107 19 -0.5851285321336661 1107 20 -0.21182574810293175 1107 21 0.24341215380391645 1107 22 -0.5272962660694134 1107 23 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0.006588803652292086 2214 6 -0.36755960608308413 2214 7 -0.5320138647823285 2214 8 -0.12532644672338478 2214 9 -0.012003925501911539 2214 10 -0.2816706079086515 2214 11 -0.1694599846716116 2214 12 -0.10953083164948652 2214 13 -0.009290666112399038 2214 14 0.048331304491469496 2214 15 -0.03479613498462086 2214 16 0.047921018741568844 2214 17 0.22261655888134158 2214 18 -0.4893294582593768 2214 19 0.07852154328111993 2214 20 0.03855132580355303 2214 21 -0.45719116444646346 2214 22 -0.12759792519459978 2214 23 0.18758061398342107 2214 24 0.19504148146030786 2214 25 0.14109688448996138 2214 26 0.022621898957503106 2214 27 -0.16672839904293824 2214 28 0.2229452498058606 2214 29 0.020207138866204843 2214 30 0.30963637197595517 2214 31 0.1878247843653753 2214 32 0.28274524279268576 2214 33 -0.05263217655215868 2214 34 0.14731012470268467 2214 35 0.43576585177964944 2214 36 0.02663922020302323 2214 37 0.001135218653281811 2214 38 0.4359617788197738 2214 39 0.06939573848087832 2214 40 0.2027480561798285 2214 41 0.46669812883710904 2214 42 -0.3408132698434869 2215 1 -0.08409199000866607 2215 2 -0.3054363067186401 2215 3 -0.3068579151524631 2215 4 -0.6186474277143716 2215 5 -0.133997527967729 2215 6 0.3263296000848186 2215 7 0.2507769214350688 2215 8 0.07665337433125075 2215 9 -0.24387142978518028 2215 10 -0.2230374459059874 2215 11 -0.4333092136836072 2215 12 -0.03785750212350081 2215 13 0.1848840911691525 2215 14 0.21290392965804017 2215 15 0.22924346143647156 2215 16 0.4776754447838343 2215 17 0.732801215268168 2215 18 0.8446701610058014 2215 19 0.363216003589514 2215 20 0.7503350684268142 2215 21 0.20395484735632152 2215 22 0.23695566323105766 2215 23 0.25592251385016596 2215 24 0.00876697165638518 2215 25 -0.11361470888525105 2215 26 0.07525848757353515 2215 27 0.23561312008898483 2215 28 -0.021200952364331727 2215 29 -0.12561429650406097 2215 30 -0.5679573839730239 2215 31 0.07693576856487994 2215 32 -0.1109345592252069 2215 33 0.10080395518208718 2215 34 0.20166002000060956 2215 35 0.26433999988549317 2215 36 0.39281093160603575 2215 37 0.33960847203230315 2215 38 0.3233594563373785 2215 39 0.18485357209976172 2215 40 0.6373568511541308 2215 41 0.5783486142739989 2215 42 0.5847530731985842 2216 1 -0.7840037287553585 2216 2 -0.6218528998342663 2216 3 -0.9704060175355167 2216 4 -0.1799889987646779 2216 5 -0.6465306259377215 2216 6 -0.9515168253344228 2216 7 -1.2591083389356257 2216 8 -0.7941321508232093 2216 9 -0.5852659575874739 2216 10 -0.5060912852705037 2216 11 -0.8550214819978166 2216 12 -0.7271778904730155 2216 13 -0.06383612065785371 2216 14 -0.29159739426075504 2216 15 0.11582775093338071 2216 16 0.36717235563996553 2216 17 0.19890889399720635 2216 18 0.31263132605434407 2216 19 0.5656873186821869 2216 20 0.12500408872690372 2216 21 -0.4318792214874563 2216 22 0.15778531901217008 2216 23 0.4086144820761106 2216 24 0.06331242620183986 2216 25 0.1603482213883609 2216 26 -0.09217310995515504 2216 27 -0.3800973830001624 2216 28 -0.18774993735991785 2216 29 5.992957289464584E-4 2216 30 -0.13706594531459615 2216 31 -0.45192566126742406 2216 32 -0.7208198195959028 2216 33 -0.575983335197435 2216 34 -0.36374156870194696 2216 35 -0.3392787115002065 2216 36 -0.12131086892353693 2216 37 0.04569849851067431 2216 38 -0.43480470959377726 2216 39 -0.6839019442285732 2216 40 -0.12345515237632299 2216 41 -0.17875641661743913 2216 42 -0.025661754691967076 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 2.0 26 12.0 27 70.0 28 149.0 29 320.0 30 534.0 31 849.0 32 1572.0 33 2388.0 34 4153.0 35 5837.0 36 7579.0 37 11651.0 38 18005.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.5097607349259239 97.16308051429755 0.7247604525517215 0.6023982982248075 2 1.5869430168859773 2.441595602492423 0.48191863857984607 95.48954274204175 3 95.31258824193822 2.0481542139643456 2.2608761130249806 0.37838143107245725 4 2.9611641347113196 92.58673594247097 3.6049773159390828 0.8471226068786355 5 3.6691893520087353 5.022779472118679 86.66930230806884 4.638728867803757 6 92.8352252404887 3.3188381242822986 0.8075902185576326 3.0383464166713727 7 5.594879518072289 2.435993975903614 83.42243975903615 8.546686746987952 8 2.823795180722892 2.5 1.5436746987951806 93.13253012048193 9 32.02876451874023 13.841983396396905 29.417744394872088 24.711507689990775 10 23.96980478530148 24.34442122701003 21.16488770919222 30.520886278496263 11 37.36162361623616 12.273138037502825 25.186384516906397 25.17885382935462 12 19.93034638554217 21.741340361445783 20.227786144578314 38.10052710843374 13 45.137542128749224 21.7845644028544 13.556513716555893 19.521379751840485 14 26.812876506024097 25.003765060240962 28.43185240963855 19.751506024096386 15 43.28878012048193 20.414156626506024 19.815512048192772 16.48155120481928 16 40.66076807228916 25.0 18.63328313253012 15.705948795180724 17 21.15399096385542 22.16867469879518 15.122364457831324 41.55496987951807 18 19.903214265539383 27.79294631592822 13.994388686990417 38.309450731541986 19 24.617354142741497 25.703635370973508 21.288852909614626 28.390157576670372 20 26.712599536906307 27.99646090999793 19.62877204871896 25.662167504376797 21 23.73825793942132 24.555260631388716 21.238305001788373 30.468176427401595 22 29.472336740648707 25.114361551928617 21.682573746729165 23.730727960693514 23 25.135068993430092 25.999134052446305 23.160332072061895 25.705464882061708 24 20.733796427025094 23.689313077690556 27.535249712919562 28.04164078236479 25 26.211855951506934 20.95028331544963 25.436268142542495 27.401592590500933 26 27.303702867039405 22.62758607706933 26.993091244517238 23.075619811374033 27 25.63769507351142 21.91788558197323 27.70655672897724 24.737862615538113 28 25.705464882061708 25.703582387379754 25.8843018768472 22.706650853711338 29 25.541687844731836 27.288642909583782 23.352346529621055 23.817322716063327 30 25.33273093503511 28.1997703356488 22.473221513149223 23.994277216166864 31 28.217243975903617 23.217243975903614 23.221009036144576 25.344503012048193 32 28.540501873082206 25.686639935242184 22.911842774044164 22.861015417631446 33 26.106436249317593 25.682874945878275 26.40198791438414 21.808700890419985 34 25.298846030759965 23.233749364658046 27.955046026994975 23.512358577587015 35 26.812371755049792 24.31994879614465 26.016076504583875 22.851602944221682 36 25.381226702812604 24.326970141948117 27.261945103354794 23.02985805188448 37 26.46787522825248 24.118521865175733 23.568833418045593 25.844769488526193 38 27.480657367142935 24.36136367914761 23.237514354021947 24.920464599687506 39 25.759201732090748 24.00451849759955 26.659135837334087 23.57714393297562 40 25.15484101733843 24.81221408535552 26.37286093488206 23.660083962423993 41 26.488582669753956 23.186686997609232 24.702095216581014 25.622635116055797 42 25.787868519146052 22.54791219548929 26.358296622613803 25.305922662750856 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 1.0 11 11.0 12 21.0 13 30.5 14 40.0 15 58.5 16 77.0 17 77.0 18 100.0 19 123.0 20 153.5 21 184.0 22 184.0 23 236.0 24 288.0 25 312.5 26 337.0 27 379.5 28 422.0 29 422.0 30 498.5 31 575.0 32 667.0 33 759.0 34 759.0 35 1040.0 36 1321.0 37 1633.0 38 1945.0 39 2252.0 40 2559.0 41 2559.0 42 2480.0 43 2401.0 44 2696.0 45 2991.0 46 3196.0 47 3401.0 48 3401.0 49 3968.0 50 4535.0 51 4793.5 52 5052.0 53 5052.0 54 5320.5 55 5589.0 56 4964.0 57 4339.0 58 4038.5 59 3738.0 60 3738.0 61 3793.0 62 3848.0 63 3197.0 64 2546.0 65 2315.0 66 2084.0 67 2084.0 68 1895.0 69 1706.0 70 1320.0 71 934.0 72 934.0 73 676.5 74 419.0 75 314.5 76 210.0 77 252.0 78 294.0 79 294.0 80 300.5 81 307.0 82 173.5 83 40.0 84 40.0 85 37.0 86 34.0 87 17.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.00564748404585757 6 0.0 7 0.0018824946819525237 8 0.0018824946819525237 9 0.0 10 0.0 11 0.009412473409762617 12 0.0018824946819525237 13 0.018824946819525235 14 0.0018824946819525237 15 0.0018824946819525237 16 0.0018824946819525237 17 0.0018824946819525237 18 0.026354925547335328 19 0.007529978727810095 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0018824946819525237 32 0.0 33 0.0 34 0.0 35 0.0 36 0.00564748404585757 37 0.0 38 0.0 39 0.01129496809171514 40 0.0037649893639050474 41 0.0 42 0.00564748404585757 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 53121.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.164304135840815 #Duplication Level Percentage of deduplicated Percentage of total 1 59.147840337118105 19.024491255812205 2 13.48472433571345 8.674535494437228 3 7.9714386047056065 7.691873270458011 4 5.407936322135081 6.957700344496527 5 3.8628116586679155 6.212232450443327 6 2.6922626711927893 5.195685322188965 7 1.6212103476530495 3.650157188305943 8 1.1003160482266183 2.831272001656595 9 0.8076788013578369 2.338058394985034 >10 3.4882359826758744 18.873891681256 >50 0.24581528736977643 5.671956476722953 >100 0.15802411330914198 9.813444777018505 >500 0.0058527449373756285 1.1144368517158938 >1k 0.0058527449373756285 1.9502644905028141 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACGAAGGCCAGGGGAAGACGTGCATGTACGGATCGTCCTCGC 1036 1.9502644905028141 No Hit ACGATGCGTTGTGTGAACACCACTGACTCCGCCGTGCGTATT 592 1.1144368517158938 No Hit ACGTGGTCCACCGGAAGAAAAGCTCGGCAAGATCTTCGAACG 474 0.8923024792454961 No Hit ACGATCTCCGCTGCGCGCTCCAGCGTCTGCTGGCGCATGTTG 364 0.6852280642307186 No Hit ACGATGTCGGCCTTGAGAGCAATCAGCTCCAAAGTTCATCCC 295 0.5553359311759944 No Hit ACGATGTCTCGACCTGTCTCAGTTATTGAAGTAGAACCAAAT 289 0.5440409630842793 No Hit ACGATGCCGTGCTCGCCACGTTCGTCGCGCTGTTCGGCGTGG 277 0.521451026900849 No Hit ACGATGTCCTGCGTGGACTCAAGCAGTCTCTCAACCCACAGG 245 0.4612111970783683 No Hit ACGATGTCGATCCTTAATCACCTCCTCTAAAGGTTTTTAAGT 236 0.4442687449407955 No Hit ACGATGTCGGTCGACCGGCATTCCGTATATCGATCAGGGCTA 231 0.43485627153103296 No Hit ACGAAGGCCAGGGGGAAGACGTGCATGTACGGATCGTCCTCG 230 0.4329737768490804 No Hit ACATTGTCGACAGCGGCGCGGCCCCTTGTGGAAGTTGTCGGC 230 0.4329737768490804 No Hit ACGATCTCCGCTAGCGCGCTCCAGCGTCTGCTGGCGCATGTT 175 0.3294365693416916 No Hit GCGAGGTCGCTGCGCCATCGCCGCGCCGGCCGTCGCGCAGTC 175 0.3294365693416916 No Hit ACGATGTATTGCGCGAGGAACAGAGCATGAGGTGCTCACCCT 172 0.32378908529583406 No Hit ACGATGTCCCGCATTTGAAACCTAGCGGAGGGAGGAGGCAGG 165 0.3106116225221664 No Hit ACGATGTCGGTCGGACCGGCATTCCGTATATCGATCAGGGCT 158 0.2974341597484987 No Hit ACGACGTCGCGCGAGGCCCGCGACCTGAAGTAGTGCGTGGCC 150 0.2823742022928785 No Hit ACGATGACACTCGCAATCTTGCCGATTGCTTAACTAAAGTTT 146 0.27484422356506844 No Hit ACGACGTCGCGCGATGCCCGCGACCTGAAGTAGTGCGTGGCC 142 0.26731424483725835 No Hit ACGATGAACAGATTTCGACATCCTGGTGCCGCGCGAGGACGC 139 0.2616667607914008 No Hit GCTATGTCCGTTTAGCAACGGATCGTCGGACTGTAGAACTCT 130 0.24472430865382805 Illumina DpnII expression Sequencing Primer (100% over 22bp) ACGATCGCTGGGGCCACGTCGGCGCCACTAGGTATCGTCCGC 127 0.2390768246079705 No Hit ACGATGCGTTGTGGTGAACACCACTGACTCCGCCGTGCGTAT 124 0.2334293405621129 No Hit ACGGCGTCGCACGCATCAAGCTGCCCGAAGGCGTAACGGTGC 118 0.22213437247039775 No Hit ACGATGTCTATCTGTGTTCTACCTTCATTTCTCAAGCAAGAC 114 0.21460439374258766 No Hit ACGATGTATGGCCACGTGGATGCTGCCCTCGGTCGGCGTCGC 104 0.19577944692306246 No Hit ACGATGCCGTGCTTCGCCACGTTCGTCGCGCTGTTCGGCGTG 102 0.1920144575591574 No Hit ACGATGTCTTCTCTGGGAAAGTTTGGGGAAGCTGGGTGTCTT 101 0.19013196287720488 No Hit ACGATGTCGTGAGTGACCATGAACCTTTAGTCTTCAGCCATG 97 0.18260198414939477 No Hit ACGGCGTCGCACAGCATCAAGCTGCCCGAAGGCGTAACGGTG 97 0.18260198414939477 No Hit ACGATGCCATACTTTGTCTTCGTCCACGGCTATAACAACAAT 95 0.17883699478548973 No Hit ACGATCTCCATGGCTGCACCATGAGGCTGTTGTGATAGATCA 94 0.1769545001035372 No Hit ACGATGCCGTGCGTCGCCACGTTCGTCGCGCTGTTCGGCGTG 87 0.16377703732986956 No Hit ACGGTGCCAGAGGCTTGGTGAGATTGGCTAAGCAATTTGGAA 87 0.16377703732986956 No Hit AAGATATCTCAGCCGACGTCTCAGTTACATTACCATGGATCT 87 0.16377703732986956 No Hit ACGAAGGCCAGGTGGAAGACGTGCATGTACGGATCGTCCTCG 85 0.1600120479659645 No Hit ACGATGCCGTGCCTCGCCACGTTCGTCGCGCTGTTCGGCGTG 85 0.1600120479659645 No Hit ACGGCGTCGCACCATCAAGCTGCCCGAAGGCGTAACGGTGCA 84 0.158129553284012 No Hit ACGACGTCGCGCGATGCCCGCGACCTGAAGGAGTGCGTGGCC 84 0.158129553284012 No Hit ACGGTGCCAGAGGGCTTGGTGAGATTGGCTAAGCAATTTGGA 83 0.15624705860205945 No Hit ACGATGTCTCGCGTCGCGAGACCCTGGCCTGGCTGGACGGCG 81 0.1524820692381544 No Hit ACATTGTCGACAGGCGGCGCGGCCCCTTGTGGAAGTTGTCGG 81 0.1524820692381544 No Hit ACGACGTCGCGCGAGGCCCGCGACCTGAAGGAGTGCGTGGCC 80 0.15059957455620188 No Hit ACGATGTCACATCAGTCCGACGATCTTGTACGTGATCGTCGG 77 0.1449520905103443 No Hit ACGATATCTATACCATCAAGCAGACTGATCCGGTTCTTGCCA 76 0.14306959582839177 No Hit ACGATGCCACCGGCTGATAGCAGTGCTTTCCCCCATGTGGCC 76 0.14306959582839177 No Hit ACGAAGGCCAGGAGGAAGACGTGCATGTACGGATCGTCCTCG 74 0.13930460646448672 No Hit ACTAGGTCGCTCAGTGAGCGATCGTCGGACTGTAGAACTCTG 74 0.13930460646448672 Illumina DpnII expression Sequencing Primer (100% over 23bp) AAGATATCTCTCGAGTTCTCCAGTCCGACGATCGCCTACGAT 74 0.13930460646448672 Illumina Small RNA Adapter 1 (95% over 21bp) ACGATGTCTTGAGAAGATCAAAGAGAAATACATTGATCAGGA 71 0.13365712241862918 No Hit ACGATGTCTCCGATCGCCAACGATGTCGGACTGTAGAACTCT 67 0.1261271436908191 Illumina NlaIII expression Adapter 1 (95% over 21bp) ACGATGTCGGGTGTGGCGGCGACGACCCATTCGAACGTCTGC 67 0.1261271436908191 No Hit ACGATGTCGCGGAGAAAAGATGGAGAAGTGGGTGGTCCCTTC 67 0.1261271436908191 No Hit ACGATGTCCCGGGTGGCGCTCTGAGTGTATCGTCCTTCTTCG 65 0.12236215432691402 No Hit ACGATGTCTCCAATGGCTCAGCGGTTAAAAGCATGAACTGTT 63 0.11859716496300898 No Hit ATGATATCAGCTGGTAAATTTGCCTGATTTCTTACAGGCTAC 63 0.11859716496300898 No Hit ACGATGTCGTGCGTGGAATGTCATGAAATAGACCAATATACA 62 0.11671467028105645 No Hit ACGATGTCCCTTGTTGACTAGGCGCATAGTCGCCATTCACAG 62 0.11671467028105645 No Hit ACGATCTCCGCTGGCGCGCTCCAGCGTCTGCTGGCGCATGTT 61 0.11483217559910394 No Hit ACGTAGTCTCGATCGAACGGAGCTTCAGTGACTACGTTCGAG 59 0.11106718623519887 No Hit ACGATGTCTCCTGAGGCACACAGCCAGCACAGTGAAGAGGCA 58 0.10918469155324637 No Hit GCTATGTCAGCGTAGCCGCTGATCGTCGGACTGTAGAACTCT 58 0.10918469155324637 Illumina DpnII expression Sequencing Primer (100% over 22bp) ACGATGTCTAACGGGTCCTCCAAGCCCTGGAGGGGCTGAAAA 56 0.10541970218934131 No Hit AAGATATCAGGCCAGTCCGACGATCGCTAACGATATCTTGCC 56 0.10541970218934131 No Hit ACGATGTCTTCTACTGGGAAAGTTTGGGGAAGCTGGGTGTCT 55 0.1035372075073888 No Hit ACGATGTCGGCCGTTGAGAGCAATCAGCTCCAAAGTTCATCC 54 0.10165471282543627 No Hit ACGTGGTCCACCGGGAAGAAAAGCTCGGCAAGATCTTCGAAC 54 0.10165471282543627 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0018824946819525235 0.0 0.0 13 0.0 0.0 0.02070744150147776 0.0 0.0 14 0.0 0.0 0.02070744150147776 0.0 0.0 15 0.0 0.0 0.07529978727810094 0.0 0.0 16 0.0 0.0 0.1430695958283918 0.0 0.0 17 0.0 0.0 0.21272189906063516 0.0 0.0 18 0.0 0.0 0.21836938310649273 0.0 0.0 19 0.0 0.0 0.22025187778844524 0.0 0.0 20 0.0 0.0 0.5327459949925641 0.0 0.0 21 0.0 0.0 1.3817510965531523 0.0 0.0 22 0.0 0.0 1.6754202669377458 0.0 0.0 23 0.0 0.0 1.8034299053105174 0.0 0.0 24 0.0 0.0 1.8260198414939477 0.0 0.0 25 0.0 0.0 1.948381995820862 0.0 0.0 26 0.0 0.0 2.053801698010203 0.0 0.0 27 0.0 0.0 2.1516914214717344 0.0 0.0 28 0.0 0.0 2.2853485438903633 0.0 0.0 29 0.0 0.0 2.415240676945088 0.0 0.0 30 0.0 0.0 2.5187778844524766 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCTGAC 20 0.0020754137 36.048115 8 TAGCGCG 20 0.0020754137 36.048115 12 TCTAGTC 20 0.0020754137 36.048115 7 CTATACC 35 6.7421934E-7 36.048115 8 TATACCC 20 0.0020754137 36.048115 9 CTCCTGA 20 0.0020754137 36.048115 8 ACTTGCA 20 0.0020754137 36.048115 7 CCTCGGG 20 0.0020754137 36.048115 8 TCGCGCG 40 4.6753485E-8 36.048115 7 TCGGACC 20 0.0020754137 36.048115 11 GAGTTCT 20 0.0020754137 36.048115 13 CCCGCCT 20 0.0020754137 36.048115 8 TCGCTCA 20 0.0020754137 36.048115 7 TCTGACA 20 0.0020754137 36.048115 9 GCAGACC 20 0.0020754137 36.048115 12 TCTTGAG 20 0.0020754137 36.048115 7 TCGTCAG 30 9.760786E-6 36.048115 7 TCGCAAT 20 0.0020754137 36.048115 11 CTTGTTG 20 0.0020754137 36.048115 10 GATTTCG 20 0.0020754137 36.048115 11 >>END_MODULE