FastQCFastQC Report
Thu 26 May 2016
SRR2099169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127809
Sequences flagged as poor quality0
Sequence length42
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCGCATCAGGCCGCGACTCTTTCAAGACCGCCGAGATCA5010.3919911743304462No Hit
GATGCTCACTACGGTGCAGAGCCCCAATTCCTACTTTATGGA4650.3638241438396357No Hit
GATGCCCGCAGGCTCGAACATGGCCATGATGATCCGCGAATG4590.35912963875783394No Hit
GAAGCTGATACCGCTCAGGTCTACCATCTCGTGCCTCGATGA2720.21281756370834606No Hit
GATGCTGACGGGCTCAGCAGCGTGCCGCCGACGGCGACCGCG2690.21047031116744516No Hit
GATGATCCCCGTACGTCCTGGTCAACAACGCCGGCGTGAACG2600.20342855354474254No Hit
GATGCTCACCGCCTTGCTCACCCCCCTTACCGCCCCCAGTCT2160.16900218294486302No Hit
GATGCGCAGGGGGCAGACTTGCTAATATACCCCGTTTCTTGC2030.1588307552676259No Hit
GATGCTCTCGGGCTGCTGAAAGCACCTTAGAATTTCTAAGTG1780.13927031742678528No Hit
GATGCTCAGATCGTTCCCCTAATCCTGTACTTCCCTGAATTC1720.13457581234498353No Hit
GACGCGCATATCCGGCAGGCTCCGTCCTTCACCGAACTGATC1640.12831647223591452No Hit
GATGCTCAGTTCGAGGAAACAGGACCAAGACCCCTGGACCTG1540.12049229709957826No Hit
GATGCTCAAAGCGTGATGTTTACACAGCAACAAAATCATATA1520.11892746207231102No Hit
GATGCTCATTTCGGTGAAGCATGTGTTGCAGAGGGCACGCAG1520.11892746207231102No Hit
GATGCTCTCGTTGGTGCAAATATCTAACCATTATACAAGGTC1470.11501537450414291No Hit
GATGCTCACCTCGGAGCCTAGGTTCTTTGAGGCCCCAGCCCC1420.11110328693597477No Hit
GAAGCTGATACCTCTCAGGTCTACCATCTCGTGCCTCGATGA1370.10719119936780665No Hit
GATGCCCGCAGGGCTCGAACATGGCCATGATGATCCGCGAAT1370.10719119936780665No Hit
GATGCTCATTGTAAACAGTCCCTCTGCCCTGGACCCCCGGCA1330.10406152931327214No Hit
GATGCGCATCAGGGCCGCGACTCTTTCAAGACCGCCGAGATC1310.10249669428600489No Hit
GATGCTGGTCGGGGGCCGGGAAGGTGAAGTCGGGCACCAGCA1280.10014944174510403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTACT309.940932E-636.01017835
GCGCGAG200.002097209236.01017416
CCAGCGG200.002097209236.01017435
AACGAGT200.002097209236.01017415
GGTGCAT200.002097209236.01017413
TCACGAC502.3646862E-1035.996096
AGCGTCA309.967664E-635.996098
GATACCT309.967664E-635.996097
CTGTCCG251.4431191E-435.9960911
CTCGTTG251.4431191E-435.996097
TCACCCG251.4431191E-435.996096
CTCAACG1900.035.996095
CAACGTT251.4431191E-435.996097
CATGCTA251.4431191E-435.996097
CAACGCG309.967664E-635.996097
ACGTTGT251.4431191E-435.996098
TCACACC502.3646862E-1035.996096
ACCGCTC309.967664E-635.9960910
CACATTG309.967664E-635.996097
CATTGCG309.967664E-635.996097
Produced by FastQC (version 0.11.5)