FastQCFastQC Report
Thu 26 May 2016
SRR2099168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235375
Sequences flagged as poor quality0
Sequence length42
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCACATTCCGCACGCCGGGCACGATAGACGCTGACTATCG20260.8607541157727031No Hit
TTCGACAGTTGGCAGTCCGACGATCGCTTATCGACAGTTTTT18190.7728093467870419No Hit
TGCTCCAAGTGAGAAGGCTAGTGCCTGTACTTTGAATGGAAA17570.7464684014869888No Hit
TGCTACAGGGCTCGAAGTTCATACAATCCTCCCACTTTTCAG17560.7460435475305364No Hit
TGCTACAGTCGCGATGGTGTGCCCATGCCTGACAAGTACTCC14000.5947955390334573No Hit
TGCTGCAGGCGCGGCGTCGTAGCGCTGTATGGCCTGGTACTT12740.541263940520446No Hit
TGCTCCAAGTGAGGAAGGCTAGTGCCTGTACTTTGAATGGAA12210.5187466808284652No Hit
TGCTCCCGGTGCTTACAGGTGCCCGCCACCATGCTCGGCTAA12180.5174721189591078No Hit
TGCTGCTGGCCGGCGCGCAAAGCCCCAGGCATGGTCGCCCCA11170.47456186935740835No Hit
TGCTACAGCCATTTGTAAGGGCGCACGGTGGATGCCTTGGCA8020.3407328730748805No Hit
TGCTACATCTCTGTTAACCTCTCTACCTTACTTGTAAGGAAC7650.32501327668613916No Hit
TGCTACAGACTTGCCATCGCCGGCCAGCCCTTCAACCTGGGA6740.28635156664896444No Hit
TTCGACAGCGCATCTGGCTAGGCCAGCTCAGGGCAGGGGGGT6590.27997875730217736No Hit
TGCGACAACCTTGCATGGCTTCGATGAAGACTTTCATATCAA6050.25703664365374407No Hit
TGCTACAGATGCAATCAACCAGCCAGGTCTCTCCATGTCGTC5970.25363781200212426No Hit
TGCTACAGCCTCGTCTAGGGGAAATGGTTGGAAACAATAATA5930.2519383961763144No Hit
CGCTACAGATACGCCTCGATCAGTCGCTGATGACATAGCGTT5470.2323951141795008No Hit
TGCTAAAGAACGGCGTAATTATTATTTATCATTTAAAAAATG5400.22942113648433352No Hit
TGCGACAACCTTAGCATGGCTTCGATGAAGACTTTCATATCA5370.22814657461497612No Hit
CGCTACAGATACGGCCTCGATCAGTCGCTGATGACATAGCGT5270.22389803505045142No Hit
CGTTACAGTCGATCGAACGGAGCTTCAGTGACTACGTTCGAG5240.22262347318109402No Hit
TGCTACAGCATGGTGAAGAGTTTAAAGAGGCTTTAATCGGTA5240.22262347318109402No Hit
TGCTGCAGGCGCGGGCGTCGTAGCGCTGTATGGCCTGGTACT4900.20817843866171004No Hit
TGCTACAGGTCTGTTCTACAGTCCGATGATCTCGAGTCGAGC4790.20350504514073287No Hit
TGCCACATTCCGCCACGCCGGGCACGATAGACGCTGACTATC4790.20350504514073287No Hit
TGCCATAGCGGCGTTTATAACGTCTATGACTGCTTGGGGGTT4640.19713223579394581No Hit
TGCTCAAGTACAGGTCAGTACCAGCAGCGCCCGACCGATCAT4440.18863515666489644No Hit
AGCTACAGGTAAAGCGACGAGAACATCTTGGCGATGTCTTCG4200.17843866171003717No Hit
TGCTAGGGGGTCGACGACCCCATCATTAACGGTAAGGCCTGC4170.17716409984067977No Hit
TGCTACAGGTAAGATCCTTCAGGCTACAGAGAAGGCTCTGGC4070.17291556027615507No Hit
TGTTACAGTGGGGACAGAAGGCTGTGTACAGCATCTCCAAAG3990.16951672862453532No Hit
TGCTACTCTTTCCTGATTGTGCCGTTATTAAGAACGGCACAA3890.16526818906001062No Hit
TGCGACAACCTTGGCATGGCTTCGATGAAGACTTTCATATCA3860.16399362719065322No Hit
TGCTACAGCCTGGACTGGCTCAGCGTGTGCCTACCCTACGCC3850.16356877323420074No Hit
TGCTGCTGGCCGGGCGCGCAAAGCCCCAGGCATGGTCGCCCC3700.1571959638874137No Hit
AGCTCCAGGTTGCCTAGGATGTGGCCACCAGGACCTCTCAGC3690.15677110993096122No Hit
TGCCACAGCTGAGCGTCCCGGCAAGTTCAGGTTCGTCTACAC3600.152947424322889No Hit
TGCTCCTGACCACGCCGACGTTGACGGCCAGCGGGGTGAGAT3540.1503983005841742No Hit
TGCTACAGCCGCATTCTTGGACCGGCGCAAGACGGACCAGAG3480.14784917684545937No Hit
TCCTACTGCATGACGATCTTTTTTGAAATGCATGGATCGTCG3310.1406266595857674No Hit
TGCTACAGGGCTGCGAAGTTCATACAATCCTCCCACTTTTCA3310.1406266595857674No Hit
TGCAACAAGAGTTCTTTGTTGCAGTCAACAAAGATGAAAAAG3190.13552841210833774No Hit
TGCTACAGCGTAGGGCGCCATGCTGAGGGGAAGGGAAGATGG3160.13425385023898034No Hit
TGCAACAAGAGTGTTTGTTGCAGTCAACAAAGATGAAAAAGG3160.13425385023898034No Hit
TGCTACTCTTTCTGATTGTGCCGTTATTAAGAACGGCACAAT3120.13255443441317047No Hit
TGCTCCAAGTGAAGAAGGCTAGTGCCTGTACTTTGAATGGAA3010.1278810408921933No Hit
TGCCATAGCGGCTCATAGACGTTTATAACGTCTATGACTGCT2990.12703133297928837No Hit
TGCTCCAAGTGACGAAGGCTAGTGCCTGTACTTTGAATGGAA2940.12490706319702603No Hit
TGCTACAGCCTCGGTCTAGGGGAAATGGTTGGAAACAATAAT2940.12490706319702603No Hit
TGCTCAAGTACAGGGTCAGTACCAGCAGCGCCCGACCGATCA2890.12278279341476367No Hit
TGCTACAGTGGGAAGTCCAATCACCAGGAATTTGAGGCAGTG2890.12278279341476367No Hit
GGCTACAGGTGAAAACGCCCTGCTGTGTTGCAGCAACGGCAT2880.12235793945831121No Hit
TGCTATAGGTGTGTTTGAGACAGTTATGTGTAAAGCAGAGAG2820.11980881571959638No Hit
TTCGACAGTTGGCCAGTCCGACGATCGCTTATCGACAGTTTT2720.1155602761550717No Hit
TGCAACAAGAGTTTTGTTGCAGTCAACAAAGATGAAAAAGGA2670.11343600637280934No Hit
AGCTACAGGTAAACATCTTGGCGATGTCTTCGTCGCTGCCAA2670.11343600637280934No Hit
TGCTACTCTTTCATTGTGCCGTTATTAAGAACGGCACAATCA2650.11258629845990441No Hit
TGCTCCCGGTGCTTTACAGGTGCCCGCCACCATGCTCGGCTA2640.11216144450345193No Hit
TGCTACAGTCGCGGATGGTGTGCCCATGCCTGACAAGTACTC2620.11131173659054701No Hit
TGCTACAATCCGGACGATTTCCTGACCGTCGCTGGCGTCCGC2620.11131173659054701No Hit
TGCTACAGTGCCGGCTTGGCCAACATGGTGAAACCCTGTCTC2620.11131173659054701No Hit
TGCTACAGTCCCCAGTCCGACGACCGTTGTGCTGTAGAACTC2590.11003717472118958No Hit
TGCTACAGCGTAGGGGCGCCATGCTGAGGGGAAGGGAAGATG2580.10961232076473712No Hit
TGCTACAATCCGGGACGATTTCCTGACCGTCGCTGGCGTCCG2550.10833775889537972No Hit
TGCTCCAAGTGATGAAGGCTAGTGCCTGTACTTTGAATGGAA2530.10748805098247478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATTAC251.4022841E-436.1878236
TGTCGTC404.6335117E-836.1878236
GACAGCG4450.036.010854
ATTCCGC2500.036.010857
AGGTCAC700.036.0108457
ACAACCC309.975305E-636.0108455
AGCGTAT309.975305E-636.0108457
AGACTCG200.002100602336.0108457
CAGCGAT1500.036.0108456
AATCCGT200.002100602336.0108457
AATCCGG1200.036.0108457
AGCCGAA200.002100602336.0108457
GGTACGT356.9310227E-736.0108458
AGTCCCG453.3905962E-936.0108457
ACACGTA200.002100602336.0108455
ATTAGGG200.002100602336.0108459
TCCGCAC1600.036.0108459
GTCTGTT309.975305E-636.0108459
AGTCAGG453.3905962E-936.0108457
CAGTTGG2850.036.0108456
Produced by FastQC (version 0.11.5)