FastQCFastQC Report
Thu 26 May 2016
SRR2099167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62970
Sequences flagged as poor quality0
Sequence length42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCACATTCCGCACGCCGGGCACGATAGACGCTGACTATCG6391.0147689375893283No Hit
TTCGACAGTTGGCAGTCCGACGATCGCTTATCGACAGTTTTT5940.9433063363506432No Hit
TGCTACAGGGCTCGAAGTTCATACAATCCTCCCACTTTTCAG4590.728918532634588No Hit
TGCTCCAAGTGAGAAGGCTAGTGCCTGTACTTTGAATGGAAA4560.7241543592186755No Hit
TGCTACAGTCGCGATGGTGTGCCCATGCCTGACAAGTACTCC3580.5685246942988725No Hit
TGCTGCAGGCGCGGCGTCGTAGCGCTGTATGGCCTGGTACTT3520.5589963474670478No Hit
TGCTCCCGGTGCTTACAGGTGCCCGCCACCATGCTCGGCTAA3360.533587422582182No Hit
TGCTCCAAGTGAGGAAGGCTAGTGCCTGTACTTTGAATGGAA3330.5288232491662697No Hit
TGCTGCTGGCCGGCGCGCAAAGCCCCAGGCATGGTCGCCCCA3280.5208829601397491No Hit
TGCTACAGCCATTTGTAAGGGCGCACGGTGGATGCCTTGGCA2580.40971891376846115No Hit
TGCTACATCTCTGTTAACCTCTCTACCTTACTTGTAAGGAAC2230.3541368905828172No Hit
TGCTACAGATGCAATCAACCAGCCAGGTCTCTCCATGTCGTC2180.34619660155629667No Hit
TGCTACAGACTTGCCATCGCCGGCCAGCCCTTCAACCTGGGA1980.3144354454502144No Hit
TTCGACAGCGCATCTGGCTAGGCCAGCTCAGGGCAGGGGGGT1950.30967127203430206No Hit
CGCTACAGATACGCCTCGATCAGTCGCTGATGACATAGCGTT1840.29220263617595676No Hit
TGCTACAGCCTCGTCTAGGGGAAATGGTTGGAAACAATAATA1810.2874384627600445No Hit
TGCTAAAGAACGGCGTAATTATTATTTATCATTTAAAAAATG1800.28585040495474034No Hit
TGCGACAACCTTGCATGGCTTCGATGAAGACTTTCATATCAA1600.2540892488486581No Hit
TGCGACAACCTTAGCATGGCTTCGATGAAGACTTTCATATCA1520.24138478640622515No Hit
TGTTACAGTGGGGACAGAAGGCTGTGTACAGCATCTCCAAAG1490.23662061299031287No Hit
TGCTAGGGGGTCGACGACCCCATCATTAACGGTAAGGCCTGC1490.23662061299031287No Hit
TTCGACAGCCGAGTGGCCATCAGGTGCTGCATCTGAGGGTCG1460.23185643957440052No Hit
TGCCACATTCCGCCACGCCGGGCACGATAGACGCTGACTATC1440.2286803239637923No Hit
TGCTACAGCATGGTGAAGAGTTTAAAGAGGCTTTAATCGGTA1420.22550420835318405No Hit
CGCTACAGATACGGCCTCGATCAGTCGCTGATGACATAGCGT1420.22550420835318405No Hit
TGCTCAAGTACAGGTCAGTACCAGCAGCGCCCGACCGATCAT1380.2191519771319676No Hit
TGCCATAGCGGCGTTTATAACGTCTATGACTGCTTGGGGGTT1360.2159758615213594No Hit
TGCTGCAGGCGCGGGCGTCGTAGCGCTGTATGGCCTGGTACT1330.21121168810544705No Hit
TGCTCCTGACCACGCCGACGTTGACGGCCAGCGGGGTGAGAT1250.19850722566301413No Hit
TGCTACAGGTCTGTTCTACAGTCCGATGATCTCGAGTCGAGC1240.19691916785771002No Hit
AGCTCCAGGTTGCCTAGGATGTGGCCACCAGGACCTCTCAGC1120.17786247419406068No Hit
AGCTACAGGTAAAGCGACGAGAACATCTTGGCGATGTCTTCG1070.1699221851675401No Hit
TGCTACAGGTAAGATCCTTCAGGCTACAGAGAAGGCTCTGGC1070.1699221851675401No Hit
TGCGACAACCTTGGCATGGCTTCGATGAAGACTTTCATATCA1060.168334127362236No Hit
TGCTGCTGGCCGGGCGCGCAAAGCCCCAGGCATGGTCGCCCC1050.16674606955693186No Hit
TGCTACTCTTTCCTGATTGTGCCGTTATTAAGAACGGCACAA1010.1603938383357154No Hit
TGCTACAGGGCTGCGAAGTTCATACAATCCTCCCACTTTTCA1000.1588057805304113No Hit
TGCCATAGCGGCTCATAGACGTTTATAACGTCTATGACTGCT1000.1588057805304113No Hit
TGCCACAGCTGAGCGTCCCGGCAAGTTCAGGTTCGTCTACAC990.1572177227251072No Hit
TGCTACAGTGGGAAGTCCAATCACCAGGAATTTGAGGCAGTG960.15245354930919486No Hit
TGCTACAGCGTAGGGGCGCCATGCTGAGGGGAAGGGAAGATG960.15245354930919486No Hit
TGCTACAGCCGCATTCTTGGACCGGCGCAAGACGGACCAGAG940.14927743369858665No Hit
GGCTACAGGTGAAAACGCCCTGCTGTGTTGCAGCAACGGCAT930.14768937589328252No Hit
TGCTACAGCCTCGGTCTAGGGGAAATGGTTGGAAACAATAAT910.1445132602826743No Hit
TGCTACAGCTCCGTGGACTCTAAGTAAGGGTAAAATGGGAGA880.13974908686676196No Hit
TGCTCCAAGTGAAGAAGGCTAGTGCCTGTACTTTGAATGGAA840.1333968556455455No Hit
TGCAACAAGAGTGTTTGTTGCAGTCAACAAAGATGAAAAAGG840.1333968556455455No Hit
TGCTACTCTTTCTGATTGTGCCGTTATTAAGAACGGCACAAT820.13022074003493728No Hit
TGCTCAAGTACAGGGTCAGTACCAGCAGCGCCCGACCGATCA820.13022074003493728No Hit
TGCTACAGTCGCGGATGGTGTGCCCATGCCTGACAAGTACTC810.12863268222963317No Hit
TGCTCCAAGTGATGAAGGCTAGTGCCTGTACTTTGAATGGAA800.12704462442432904No Hit
TGCTACAATCCGGGACGATTTCCTGACCGTCGCTGGCGTCCG790.12545656661902493No Hit
TGCTACTCTTTCATTGTGCCGTTATTAAGAACGGCACAATCA790.12545656661902493No Hit
TTCGACAGTTGGCCAGTCCGACGATCGCTTATCGACAGTTTT790.12545656661902493No Hit
TGCTCCCGGTGCTTTACAGGTGCCCGCCACCATGCTCGGCTA780.12386850881372083No Hit
TGCAACAAGAGTTCTTTGTTGCAGTCAACAAAGATGAAAAAG780.12386850881372083No Hit
TGCTATAGGTGTGTTTGAGACAGTTATGTGTAAAGCAGAGAG780.12386850881372083No Hit
TGCTACAGTCCCCAGTCCGACGACCGTTGTGCTGTAGAACTC760.12069239320311258No Hit
CGTTACAGTCGATCGAACGGAGCTTCAGTGACTACGTTCGAG750.11910433539780849No Hit
TGCTACAGCGTAGGGCGCCATGCTGAGGGGAAGGGAAGATGG730.11592821978720026No Hit
AGCTACAGGTAAACATCTTGGCGATGTCTTCGTCGCTGCCAA730.11592821978720026No Hit
TGCTTCAGTAGGCATTGCGTGATTGCTGCGCAATGTGGCATT730.11592821978720026No Hit
TGCAACAAGAGTTTTGTTGCAGTCAACAAAGATGAAAAAGGA710.11275210417659202No Hit
TGCCATAGCGGCCAGTCATAGACGTTTATAACGTCTATGACT700.11116404637128792No Hit
TGCTCCAAGTGACGAAGGCTAGTGCCTGTACTTTGAATGGAA700.11116404637128792No Hit
TGCTACAGTTCGGCGGCGTCGCCAGGAGGAGCGCGCGGGCAC700.11116404637128792No Hit
TGCTGCTGCTTGAGATGAGAAGGAGAAGGAAAAGGAGGCGGA670.10639987295537558No Hit
TGCTCCCGGTGCGTTACAGGTGCCCGCCACCATGCTCGGCTA670.10639987295537558No Hit
TGCTACAGGGCGCAAGCCTAGATTTGAACTAACCACTCAGCT660.10481181515007146No Hit
CGCTACAGATACTGAAACCCTGGCCTCGATCAGTCGCTGATG650.10322375734476735No Hit
TGCTACAGTTAGAATGCAGACCATGGGGGGCAAGAAGTTCAA650.10322375734476735No Hit
TGCTACTCTTTCTTGTGCCGTTATTAAGAACGGCACAATCAG640.10163569953946323No Hit
TGTTACGGTCGAAAGGCAGAGAGCCCTGCATCAGCTCAGGCT640.10163569953946323No Hit
TGCTACAGCCTGGACTGGCTCAGCGTGTGCCTACCCTACGCC630.10004764173415913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCC200.00209204736.06
CAGCTCC251.4338933E-436.06
CTACAGC7950.036.03
ACAGCGT1000.036.05
ACAGCGA650.036.05
CTCGAAG502.3283064E-1036.011
ACAGTGG1400.036.05
CTACACC356.8403097E-736.03
CGACAAC1100.036.03
ACAGCAC453.3160177E-936.05
ACAGCAA251.4338933E-436.05
AACGTTT200.00209204736.09
ACAGTAG309.880096E-636.05
AGGGGGT404.7548383E-836.05
GAGCGTC200.00209204736.011
GGTTGTT200.00209204736.07
CAAGAGT750.036.06
TGCATGG356.8403097E-736.012
AATCCGG404.7548383E-836.07
GGGTCGA200.00209204736.08
Produced by FastQC (version 0.11.5)