FastQCFastQC Report
Thu 26 May 2016
SRR2099166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197955
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTACGTCGGGAGACGCAGCACTTTGACCCAGTCCGTGTCG12650.6390341239170518No Hit
AACTACGTAACTGCCAGTGGTACCCAGTACCTCTTGTATATA11320.5718471369755752No Hit
GACTACGTAGTGACAGGCGCGGTGGCTCACGCCTGTAATCCC10070.5087014725568942No Hit
CACTACGATGACGGGCAGCGCGTGATAGCAGCGATGCGGCTC9160.4627314288600945No Hit
GGCAACGTATTCGGGCACAGAAACCACGCGGGTTGGCGAGCA6350.32077997524689955No Hit
GACTACAAACGAGAAGGCCAGGAAGAGAAGCCAGAATATTTA5980.30208885857896994No Hit
GAGGACGTGTGGGAACAGGGACTGTGTCTGCTTCTAGATGCA5430.27430476623475036No Hit
CACTACGATGACGGGGCAGCGCGTGATAGCAGCGATGCGGCT5310.26824278245055694No Hit
GACTACGTTCCGACTACGTTTTTTTTTTTTTTTTTTTTTTTT5000.25258265767472404No Hit
GGCAACGTATTCGGCACAGAAACCACGCGGGTTGGCGAGCAG4450.22479856533050444No Hit
GACTACGTCCCGGAGATGGCTCAGTGGGTAAGAGCACCCGAC4390.22176757343840772No Hit
GTCTACGAACATGTTGCGGTATGCTTCGTCGTTGCTCCATTC4220.2131797630774671No Hit
GACTACGTGGTGGTTTAATAGTAAAAGACTGGTTAATGATAA3760.1899421585713925No Hit
GACTACGTAGGAGATAAAGGTCTTTGCAGTGATGACAGAAAG3600.18185951352580132No Hit
GACTACGCGCAATCCGACGATCTTAGGACTCTGAACCCCTAT3380.17074587658811347No Hit
GACTACGTCATGACGATCGGAGGACTACGTTTTTTTTTTTTT3330.16822005001136622No Hit
AACTACGTAACTGGCCAGTGGTACCCAGTACCTCTTGTATAT2880.14548761082064104No Hit
GAGGACGTGTGGAGAACAGGGACTGTGTCTGCTTCTAGATGC2730.13791013109039932No Hit
GACTACGTGGTAAGTCACTCAAAAGTAAGTGAGTGACTTAGG2690.13588946982900155No Hit
GACTACGTAGGAATAAAGGTCTTTGCAGTGATGACAGAAAGA2570.12982748604480815No Hit
GACTACGTTGCCGATCAGTCGGTACTCCCTGGCATCTCACTT2520.12730165946806093No Hit
GACTACGTGGTACCTAAGTCACTCAAAAGTAAGTGAGTGACT2470.12477583289131368No Hit
GACTACGTGTGCGGCCTTTATGAGTCACACCCTGGAAAGGAA2320.11719835316107195No Hit
GGCAACGTATTCCGCGTGGCACAGAAACCACGCGGGTTGGCG2240.11315703063827637No Hit
GAGGACGTGTGGAGCTTCTTGAGAACAGGGACTGTGTCTGCT2200.11113636937687858No Hit
GTCCACGTAGTCCAGCTTGCACTGATCGTCGGACTGTAGAAC2150.10861054280013134No Hit
GCCTATGTCATGCTTATTCTTCTAACTACCAGTCCAAAACTA2140.1081053774847819No Hit
GACTACATGGTGACCAAAAATAATGAACTTAAAACAAAAATA2070.10456922027733576No Hit
GACTACGTTAGAACAGTCCGACGATCGCCGGACTGTAGAACT2020.10204339370058853No Hit
GACTACGTACGTCGAAAAATTACACGCGGAGGTTCATGGAAA2020.10204339370058853No Hit
GTCTACGAACATGGTTGCGGTATGCTTCGTCGTTGCTCCATT2010.10153822838523906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCA1900.036.0280845
TCACGGA200.002094867436.028088
TCACGCG356.8976806E-736.028088
CTTCGTA2250.036.028083
CTTGTAC356.8976806E-736.028086
GTTGACA356.8976806E-736.028087
CTATGTG650.036.028083
TGTCACG200.002094867436.028088
CGGGTCA453.3687684E-936.028086
AGTCCAG251.4384423E-436.028089
CGTATTG800.036.028086
CGTATGG700.036.028086
CGTATCT200.002094867436.028086
CGTATCA601.8189894E-1236.028086
CGTATAG850.036.028086
CGTATAA356.8976806E-736.028086
CGACGTG309.934616E-636.028083
CGACGTC251.4384423E-436.028083
ACGTTTA2400.036.028085
TTTACGG251.4384423E-436.028088
Produced by FastQC (version 0.11.5)