##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099166_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 197955 Sequences flagged as poor quality 0 Sequence length 42 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.815341870627165 37.0 35.0 37.0 32.0 37.0 2 33.34452779672148 35.0 33.0 36.0 28.0 37.0 3 34.50810537748478 35.0 35.0 37.0 32.0 37.0 4 34.70191710237175 35.0 35.0 37.0 32.0 37.0 5 34.60177313025687 37.0 34.0 39.0 28.0 39.0 6 36.3791114142103 37.0 37.0 39.0 32.0 39.0 7 36.327074335076155 39.0 37.0 39.0 32.0 39.0 8 36.162663231542524 39.0 35.0 39.0 31.0 39.0 9 31.035108989416788 31.0 31.0 34.0 28.0 34.0 10 31.277320603167386 31.0 31.0 34.0 28.0 34.0 11 31.153590462478846 31.0 31.0 34.0 28.0 34.0 12 34.73258063701346 37.0 35.0 37.0 32.0 37.0 13 31.36943749842136 31.0 31.0 34.0 30.0 34.0 14 31.3805561870122 31.0 31.0 34.0 30.0 34.0 15 31.296835139299336 31.0 31.0 34.0 28.0 34.0 16 34.71208102851658 35.0 35.0 37.0 32.0 37.0 17 34.97067010179081 35.0 35.0 37.0 32.0 37.0 18 34.76554772549317 35.0 35.0 37.0 32.0 37.0 19 34.56152155792983 35.0 35.0 37.0 30.0 37.0 20 34.63749842135839 35.0 35.0 37.0 31.0 37.0 21 36.205673006491374 37.0 35.0 39.0 32.0 39.0 22 36.45297163496754 38.0 35.0 39.0 32.0 39.0 23 36.663933722310624 38.0 35.0 39.0 32.0 39.0 24 36.832360890101285 38.0 37.0 39.0 33.0 39.0 25 36.86706574726579 38.0 37.0 39.0 33.0 39.0 26 38.184466166553 40.0 38.0 40.0 34.0 41.0 27 38.29416281478114 40.0 38.0 40.0 34.0 41.0 28 38.36269354146145 40.0 38.0 40.0 34.0 41.0 29 38.4323154252229 40.0 38.0 41.0 35.0 41.0 30 38.46257987926549 40.0 38.0 41.0 35.0 41.0 31 38.524588921724636 40.0 38.0 41.0 35.0 41.0 32 38.55016544164078 40.0 38.0 41.0 35.0 41.0 33 38.54035007956354 40.0 38.0 41.0 35.0 41.0 34 38.51639514030967 40.0 38.0 41.0 35.0 41.0 35 38.45178449647647 40.0 38.0 41.0 35.0 41.0 36 38.414013285847794 40.0 38.0 41.0 34.0 41.0 37 38.38600692076482 40.0 38.0 41.0 34.0 41.0 38 38.27970498345584 40.0 38.0 41.0 34.0 41.0 39 38.232239650425605 40.0 38.0 41.0 34.0 41.0 40 38.229309691596576 40.0 38.0 41.0 34.0 41.0 41 38.001727665378496 40.0 38.0 41.0 34.0 41.0 42 38.09804753605617 40.0 38.0 41.0 34.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3771571810729242 1101 2 1.137663954911858 1101 3 0.6270301468282753 1101 4 0.6620480948440317 1101 5 1.217070458560805 1101 6 0.5698101404584861 1101 7 0.37653799760322926 1101 8 0.34099892543388677 1101 9 0.3193740801167415 1101 10 0.39171469395083136 1101 11 0.3532489193679673 1101 12 0.41923006456695333 1101 13 0.16735383061626052 1101 14 0.35082189383309625 1101 15 0.24456748273325957 1101 16 0.4381447972202537 1101 17 0.20175659723463468 1101 18 0.017135697828678076 1101 19 -0.21443266632963542 1101 20 0.0685308873588113 1101 21 0.30656686619006734 1101 22 -0.3934511334247617 1101 23 -0.032296775195419514 1101 24 0.13845720851887222 1101 25 -0.1253815156093765 1101 26 0.14054539944705624 1101 27 0.056624877119183736 1101 28 0.46532498313864323 1101 29 0.3092374274807952 1101 30 0.4622848208483745 1101 31 0.46108954730305385 1101 32 0.44047468645378274 1101 33 0.33469041369536257 1101 34 0.3009669715544945 1101 35 0.5296283191868554 1101 36 0.5446085628097848 1101 37 0.45650608571605744 1101 38 0.40390661710140563 1101 39 0.5282366389203474 1101 40 0.3732872243744012 1101 41 0.70507838617484 1101 42 0.7417334899567081 1102 1 0.3616960117109187 1102 2 0.24457705247716888 1102 3 0.16268857938558767 1102 4 0.019138906999714322 1102 5 -0.18395140033381097 1102 6 0.24588087086544874 1102 7 0.3003428669830015 1102 8 0.45561595049164083 1102 9 -0.09602488913863283 1102 10 0.22920358678893393 1102 11 0.00332000520411313 1102 12 0.09320376280758325 1102 13 0.08857295270618337 1102 14 0.3171627504174239 1102 15 0.3739703617096204 1102 16 0.042187804151119224 1102 17 0.053720343458202535 1102 18 0.07567488389585009 1102 19 0.09465743496767942 1102 20 0.038532664504714376 1102 21 -0.03628012154762672 1102 22 0.06027198724344629 1102 23 0.04732824701890337 1102 24 0.20928535850998742 1102 25 0.15780668414182486 1102 26 0.369663935078826 1102 27 0.40028935197256743 1102 28 0.11686221434532484 1102 29 0.0012491566437589086 1102 30 0.0683448883799187 1102 31 0.40028450020869144 1102 32 0.3403947148881201 1102 33 0.16157863121802052 1102 34 0.2314361380730645 1102 35 0.35641879368481 1102 36 0.20129063140761616 1102 37 0.2754504610492674 1102 38 0.2839314971440601 1102 39 0.06730719161272702 1102 40 0.30125061516878304 1102 41 0.7151548034486979 1102 42 0.618559507372737 1103 1 0.0806701867467865 1103 2 0.669828128517338 1103 3 0.5191806884243277 1103 4 0.40350045649756083 1103 5 0.692017035821145 1103 6 0.6400702539357539 1103 7 0.7616054207850169 1103 8 0.6540377582391343 1103 9 0.38058917111961676 1103 10 0.2852652797588071 1103 11 0.3826959019042562 1103 12 0.00952370541877201 1103 13 0.2636805567234006 1103 14 0.32078541899705115 1103 15 0.16428600016012496 1103 16 0.5973169289717788 1103 17 0.27628131906795517 1103 18 -0.06406686646282367 1103 19 -0.037695506208791585 1103 20 -0.007485986858270621 1103 21 0.3569408253677224 1103 22 0.0493681134988222 1103 23 -0.026272900757277284 1103 24 0.12313040872519565 1103 25 0.018638821874965572 1103 26 0.11625647453363541 1103 27 0.20922048540154492 1103 28 0.27475317560787715 1103 29 0.24289191130660015 1103 30 0.2660852040183741 1103 31 0.2961238115429765 1103 32 0.272819392076066 1103 33 0.4798713141448445 1103 34 0.3270243733671805 1103 35 0.2825665698684574 1103 36 0.34921028707476154 1103 37 0.53430985846677 1103 38 0.7085728170866687 1103 39 0.6295740495754174 1103 40 0.3819177600755239 1103 41 0.5308649899623248 1103 42 0.2944562075162125 1104 1 0.7424477393360647 1104 2 0.7270648326895355 1104 3 0.6048173506648737 1104 4 0.5096748921674177 1104 5 0.3440192721758848 1104 6 0.2509794719104548 1104 7 0.3942666829277215 1104 8 0.20615530663524595 1104 9 0.5042700712523995 1104 10 0.4017217057843858 1104 11 0.5023845593925493 1104 12 0.5433048920845067 1104 13 0.17431372465966533 1104 14 0.3339502103870835 1104 15 0.14139830709981283 1104 16 0.34860108579615456 1104 17 0.40615587354416505 1104 18 0.12259886838936751 1104 19 0.1843675310404933 1104 20 0.018677616468309566 1104 21 -0.21531265146503387 1104 22 -0.06241468054687971 1104 23 -0.08918784939100277 1104 24 0.22774987898866783 1104 25 0.1914321687196079 1104 26 0.2832405405060996 1104 27 0.3279482092117405 1104 28 0.34224566285366365 1104 29 0.29940354733986396 1104 30 0.29930730199866673 1104 31 0.19361839537133818 1104 32 0.41166557280148197 1104 33 0.13493106674810207 1104 34 0.3345458633385263 1104 35 0.4041770947129564 1104 36 0.46880285119524956 1104 37 0.481956444531491 1104 38 0.4853304521415325 1104 39 0.17742854674503405 1104 40 0.3303505646041458 1104 41 0.796344757940517 1104 42 0.814059254549548 1105 1 -0.2309468091061646 1105 2 0.20606204993436705 1105 3 0.1275046499060153 1105 4 -0.14596534795875726 1105 5 0.08208521604184682 1105 6 -0.04760107522710655 1105 7 0.11348916296537226 1105 8 -0.4455791673249365 1105 9 0.12988628224634624 1105 10 0.05708119316289739 1105 11 0.22112810827243123 1105 12 0.13310035545040222 1105 13 0.20047327479467114 1105 14 0.3696801262438356 1105 15 0.3198001633709211 1105 16 0.21015695146422786 1105 17 0.19781256247837575 1105 18 0.07587830820505559 1105 19 -0.013199828975359651 1105 20 0.1664526509430928 1105 21 -0.05401194049998992 1105 22 0.01489141426497298 1105 23 -0.20963903557136376 1105 24 -0.04844476523980035 1105 25 0.27536898283651823 1105 26 0.0699097394524486 1105 27 0.1903215608215234 1105 28 0.09848621841381089 1105 29 0.27771126886617026 1105 30 0.22276089109224984 1105 31 0.06506666667758054 1105 32 0.24725180931090307 1105 33 -0.08308995667823638 1105 34 0.34539613129225444 1105 35 0.2852287614759632 1105 36 0.5661745100311109 1105 37 0.4321888912916805 1105 38 0.48374502486062454 1105 39 0.22005341978699278 1105 40 0.16935537868802442 1105 41 0.6822848254863274 1105 42 0.5739248235280172 1106 1 0.15189764477207746 1106 2 -0.013513265803119623 1106 3 -0.09417694302734247 1106 4 -0.17300406917092914 1106 5 6.261700711220897E-4 1106 6 0.22609250226022226 1106 7 0.2323958581511434 1106 8 0.35149163787707494 1106 9 0.03634471592718924 1106 10 -0.06802187563399542 1106 11 -0.0608109729661912 1106 12 -0.027001203031446153 1106 13 -0.05558635407601997 1106 14 -0.005416819654232796 1106 15 0.11643828372261922 1106 16 0.31200116859037763 1106 17 0.3568364907669874 1106 18 0.395754870564744 1106 19 0.09403253911698783 1106 20 0.20973746370484747 1106 21 0.10213014340821758 1106 22 0.20203169395899323 1106 23 0.1589513098417683 1106 24 0.17301640333585055 1106 25 0.020746194223477232 1106 26 -0.022562360538564974 1106 27 0.18492524599022886 1106 28 0.1897500663699887 1106 29 0.3987079135176117 1106 30 0.13535038746341144 1106 31 0.2678815673641637 1106 32 0.30997478487645225 1106 33 0.14713103915003245 1106 34 -0.052224918417522304 1106 35 -0.04752221614688068 1106 36 0.09040077971623361 1106 37 -0.2303985641132016 1106 38 -0.015801880626312936 1106 39 0.10779211079285744 1106 40 0.32392183663187524 1106 41 0.6636467214623565 1106 42 0.39947935406495816 1107 1 0.2604801063543647 1107 2 0.4869694284219577 1107 3 0.3543251222709003 1107 4 0.10403867696356173 1107 5 -0.27187450253558154 1107 6 0.1804027242995616 1107 7 0.10916639639476955 1107 8 0.39661167839174283 1107 9 0.11628204480813054 1107 10 0.30858248918788433 1107 11 0.1970268734262639 1107 12 0.24451825508494096 1107 13 0.1635647371294482 1107 14 0.260508133263297 1107 15 0.17750305970502112 1107 16 0.30441258233054924 1107 17 0.2827706227878082 1107 18 0.20068309947258456 1107 19 0.21649443792528444 1107 20 -0.15381041797967043 1107 21 0.008511202803340723 1107 22 0.19536893104889685 1107 23 -0.1406277174897994 1107 24 0.11400109955501136 1107 25 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2216 5 1.5476425275599297 2216 6 1.1138441852234564 2216 7 1.1898115576850898 2216 8 0.05090374302800882 2216 9 0.6480295068132946 2216 10 0.46029150582751654 2216 11 0.7466153878733088 2216 12 0.590136084123202 2216 13 0.36681457193451905 2216 14 0.5702936802086924 2216 15 0.7209343085786912 2216 16 0.572959467655231 2216 17 0.5483439993721788 2216 18 0.3576483184309609 2216 19 0.8171432034686248 2216 20 0.3151930060606958 2216 21 0.8635301251316889 2216 22 0.6432574394888562 2216 23 0.771458544299108 2216 24 0.7711765666061297 2216 25 0.418717500081101 2216 26 0.9896829104108988 2216 27 0.8674620274943905 2216 28 0.6560146495129402 2216 29 0.8533364431712727 2216 30 0.633873035122491 2216 31 0.4220745065337965 2216 32 0.21164708680462496 2216 33 0.7734065882072656 2216 34 0.3767555561628768 2216 35 0.7137559220845588 2216 36 0.6823469248927552 2216 37 0.6058742434654647 2216 38 0.437821440218066 2216 39 0.30889479439576917 2216 40 0.21462822427694306 2216 41 0.9567107806782928 2216 42 1.052651853988806 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 23.0 26 130.0 27 545.0 28 1229.0 29 2229.0 30 3561.0 31 5259.0 32 7426.0 33 10417.0 34 16072.0 35 26670.0 36 44096.0 37 58673.0 38 21624.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 92.1969758227541 3.1520376809250426 0.9061413438990864 3.744845152421768 2 3.644428278419367 93.18501005324684 2.04197104260758 1.1285906257262082 3 2.9289484983961 1.30888333207042 1.0017428203379557 94.76042534919551 4 2.0989618852769567 2.1575610618574927 93.89002551084843 1.853451542017125 5 1.55642219909676 95.88843872818937 1.9691443466663971 0.5859947260474656 6 1.1649229867695867 0.9482048769152273 1.2861638873874102 96.60070824892777 7 91.72080382311042 6.4595815189387435 1.042666478070663 0.7769481798801742 8 1.3189999646380706 5.309846276641425 92.01376084222012 1.3573929165003815 9 31.146852793161873 27.896720026300482 22.54710062463647 18.40932655590117 10 35.083242806255214 18.39122855771417 26.97572190081601 19.549806735214613 11 30.226435497971128 25.93118503433674 23.31613548735933 20.526243980332808 12 31.028906407923994 23.49454214675561 21.99615928845765 23.480392156862745 13 35.49319401356145 25.896601554210413 16.451590084582193 22.158614347645948 14 28.90803000833565 26.153224380509737 21.86668013842229 23.072065472732326 15 30.2268720352811 25.654069400312196 22.88421999161417 21.23483857279253 16 28.193418673025043 28.09743677824141 21.860634288774158 21.848510259959383 17 21.83285005607363 28.129262353880197 20.45677278559665 29.58111480444952 18 22.98866834056613 25.802132958810958 18.802572483719896 32.406626216903014 19 28.356007294734553 24.094346580719474 23.92410242938909 23.625543695156882 20 25.38506226162512 26.979869162183324 22.785986714152205 24.849081862039352 21 22.722335884418175 24.59498370841858 25.1243969589048 27.558283448258443 22 26.025106716172868 26.21252304816751 23.777121062867824 23.985249172791796 23 23.77105907908363 28.515571720845646 25.661387689121263 22.05198151094946 24 22.695056957389305 25.816473440933546 28.27561819605466 23.21285140562249 25 23.357347101676247 24.611754958523203 26.66084004405331 25.370057895747237 26 23.88371094440656 26.975322674345183 26.44338359728221 22.697582783966052 27 26.007425930135636 26.266575736909903 26.675254477027604 21.05074385592685 28 24.039301861534188 28.21752418478947 25.077416584577307 22.665757369099037 29 24.93495996564876 26.378722436917478 24.76017276653785 23.92614483089591 30 26.248894950872675 25.80232881210376 25.600262685963983 22.348513551059586 31 25.458311232350788 28.882826905104697 23.98272334621505 21.67613851632947 32 23.001692303806422 26.436816448182665 26.500972443232047 24.060518804778862 33 22.74669872798731 25.657475979267915 28.637461228366185 22.958364064378593 34 25.109747164759668 26.91975448965674 28.31704175191331 19.653456593670278 35 23.80844131241949 27.00765325452754 27.79166982394989 21.39223560910308 36 22.511795669701044 29.340654899623146 27.052749628701616 21.094799801974197 37 25.320401101260387 27.523427041499332 25.145613902149478 22.010557955090803 38 22.70162410648885 29.618347604253493 26.050364981940337 21.62966330731732 39 22.53615757275717 31.054846352416988 25.750556950387214 20.65843912443863 40 22.62343776837043 27.155803873627203 26.340968103700863 23.879790254301504 41 20.205878314117008 26.45495648989681 30.057826986944423 23.28133820904176 42 21.475587886135738 28.683286605541664 26.445404258543608 23.39572124977899 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 15.0 9 29.0 10 29.0 11 66.0 12 103.0 13 249.0 14 395.0 15 590.0 16 785.0 17 785.0 18 1003.5 19 1222.0 20 1235.0 21 1248.0 22 1248.0 23 1802.5 24 2357.0 25 2570.0 26 2783.0 27 3143.0 28 3503.0 29 3503.0 30 4026.0 31 4549.0 32 5102.0 33 5655.0 34 5655.0 35 5817.5 36 5980.0 37 7092.0 38 8204.0 39 9181.0 40 10158.0 41 10158.0 42 12290.0 43 14422.0 44 13821.5 45 13221.0 46 14586.0 47 15951.0 48 15951.0 49 17445.0 50 18939.0 51 18874.5 52 18810.0 53 18810.0 54 18530.0 55 18250.0 56 15870.5 57 13491.0 58 11760.5 59 10030.0 60 10030.0 61 10351.0 62 10672.0 63 9810.0 64 8948.0 65 6082.5 66 3217.0 67 3217.0 68 2528.0 69 1839.0 70 1701.5 71 1564.0 72 1564.0 73 1169.0 74 774.0 75 582.0 76 390.0 77 330.5 78 271.0 79 271.0 80 224.0 81 177.0 82 92.0 83 7.0 84 7.0 85 4.5 86 2.0 87 5.0 88 8.0 89 4.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04192872117400419 2 0.004546487838145033 3 0.0 4 0.0 5 5.051653153494481E-4 6 0.0010103306306988961 7 5.051653153494481E-4 8 0.0010103306306988961 9 0.12123967568386754 10 0.020206612613977923 11 0.031320249551665784 12 0.037887398651208604 13 0.020711777929327373 14 0.005051653153494481 15 0.0010103306306988961 16 5.051653153494481E-4 17 5.051653153494481E-4 18 0.007072314414892273 19 0.0020206612613977923 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.006567149099542825 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 5.051653153494481E-4 34 0.0 35 0.0 36 5.051653153494481E-4 37 0.0 38 0.0 39 0.0010103306306988961 40 5.051653153494481E-4 41 0.6738905306761638 42 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 42 197955.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.07006642923897 #Duplication Level Percentage of deduplicated Percentage of total 1 58.80016276861666 15.329241494279003 2 11.994496870579573 6.253946604026167 3 6.851783672757572 5.358793665226946 4 4.480012401418413 4.671768836351696 5 3.2437460034491448 4.22823368947488 6 2.290386963008894 3.582632416458286 7 1.7982056697734803 3.281553888510015 8 1.2924603251496891 2.695562122704655 9 1.1258162652353363 2.6415094339622645 >10 7.590055612610692 34.677578237478215 >50 0.34878989284399403 6.240812305827082 >100 0.1685817815412638 7.690636760879999 >500 0.00968860813455539 1.628147811371271 >1k 0.005813164880733234 1.7195827334495213 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGCTACGTCGGGAGACGCAGCACTTTGACCCAGTCCGTGTCG 1265 0.6390341239170518 No Hit AACTACGTAACTGCCAGTGGTACCCAGTACCTCTTGTATATA 1132 0.5718471369755752 No Hit GACTACGTAGTGACAGGCGCGGTGGCTCACGCCTGTAATCCC 1007 0.5087014725568942 No Hit CACTACGATGACGGGCAGCGCGTGATAGCAGCGATGCGGCTC 916 0.4627314288600945 No Hit GGCAACGTATTCGGGCACAGAAACCACGCGGGTTGGCGAGCA 635 0.32077997524689955 No Hit GACTACAAACGAGAAGGCCAGGAAGAGAAGCCAGAATATTTA 598 0.30208885857896994 No Hit GAGGACGTGTGGGAACAGGGACTGTGTCTGCTTCTAGATGCA 543 0.27430476623475036 No Hit CACTACGATGACGGGGCAGCGCGTGATAGCAGCGATGCGGCT 531 0.26824278245055694 No Hit GACTACGTTCCGACTACGTTTTTTTTTTTTTTTTTTTTTTTT 500 0.25258265767472404 No Hit GGCAACGTATTCGGCACAGAAACCACGCGGGTTGGCGAGCAG 445 0.22479856533050444 No Hit GACTACGTCCCGGAGATGGCTCAGTGGGTAAGAGCACCCGAC 439 0.22176757343840772 No Hit GTCTACGAACATGTTGCGGTATGCTTCGTCGTTGCTCCATTC 422 0.2131797630774671 No Hit GACTACGTGGTGGTTTAATAGTAAAAGACTGGTTAATGATAA 376 0.1899421585713925 No Hit GACTACGTAGGAGATAAAGGTCTTTGCAGTGATGACAGAAAG 360 0.18185951352580132 No Hit GACTACGCGCAATCCGACGATCTTAGGACTCTGAACCCCTAT 338 0.17074587658811347 No Hit GACTACGTCATGACGATCGGAGGACTACGTTTTTTTTTTTTT 333 0.16822005001136622 No Hit AACTACGTAACTGGCCAGTGGTACCCAGTACCTCTTGTATAT 288 0.14548761082064104 No Hit GAGGACGTGTGGAGAACAGGGACTGTGTCTGCTTCTAGATGC 273 0.13791013109039932 No Hit GACTACGTGGTAAGTCACTCAAAAGTAAGTGAGTGACTTAGG 269 0.13588946982900155 No Hit GACTACGTAGGAATAAAGGTCTTTGCAGTGATGACAGAAAGA 257 0.12982748604480815 No Hit GACTACGTTGCCGATCAGTCGGTACTCCCTGGCATCTCACTT 252 0.12730165946806093 No Hit GACTACGTGGTACCTAAGTCACTCAAAAGTAAGTGAGTGACT 247 0.12477583289131368 No Hit GACTACGTGTGCGGCCTTTATGAGTCACACCCTGGAAAGGAA 232 0.11719835316107195 No Hit GGCAACGTATTCCGCGTGGCACAGAAACCACGCGGGTTGGCG 224 0.11315703063827637 No Hit GAGGACGTGTGGAGCTTCTTGAGAACAGGGACTGTGTCTGCT 220 0.11113636937687858 No Hit GTCCACGTAGTCCAGCTTGCACTGATCGTCGGACTGTAGAAC 215 0.10861054280013134 No Hit GCCTATGTCATGCTTATTCTTCTAACTACCAGTCCAAAACTA 214 0.1081053774847819 No Hit GACTACATGGTGACCAAAAATAATGAACTTAAAACAAAAATA 207 0.10456922027733576 No Hit GACTACGTTAGAACAGTCCGACGATCGCCGGACTGTAGAACT 202 0.10204339370058853 No Hit GACTACGTACGTCGAAAAATTACACGCGGAGGTTCATGGAAA 202 0.10204339370058853 No Hit GTCTACGAACATGGTTGCGGTATGCTTCGTCGTTGCTCCATT 201 0.10153822838523906 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.003536157207446137 0.0 0.0 12 0.0 0.0 0.020711777929327373 0.0 0.0 13 0.0 0.0 0.02929958829026799 0.0 0.0 14 0.0 0.0 0.05506301937308984 0.0 0.0 15 0.0 0.0 0.09396074865499734 0.0 0.0 16 0.0 0.0 0.11719835316107197 0.0 0.0 17 0.0 0.0 0.1293223207294587 0.0 0.0 18 0.0 0.0 0.17377686848021015 0.0 0.0 19 0.0 0.0 0.20206612613977926 0.0 0.0 20 0.0 0.0 0.3707913414664949 0.0 0.0 21 0.0 0.0 0.47637089237452956 0.0 0.0 22 0.0 0.0 1.1396529514283549 0.0 0.0 23 0.0 0.0 1.4366901568538304 0.0 0.0 24 0.0 0.0 1.7382738501174508 0.0 0.0 25 0.0 0.0 1.840822409133389 0.0 0.0 26 0.0 0.0 1.9090197267055644 0.0 0.0 27 0.0 0.0 1.9534742744563158 0.0 0.0 28 0.0 0.0 2.0085372938294057 0.0 0.0 29 0.0 0.0 2.0883534136546187 0.0 0.0 30 0.0 0.0 2.3060796645702304 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTGCA 190 0.0 36.028084 5 TCACGGA 20 0.0020948674 36.02808 8 TCACGCG 35 6.8976806E-7 36.02808 8 CTTCGTA 225 0.0 36.02808 3 CTTGTAC 35 6.8976806E-7 36.02808 6 GTTGACA 35 6.8976806E-7 36.02808 7 CTATGTG 65 0.0 36.02808 3 TGTCACG 20 0.0020948674 36.02808 8 CGGGTCA 45 3.3687684E-9 36.02808 6 AGTCCAG 25 1.4384423E-4 36.02808 9 CGTATTG 80 0.0 36.02808 6 CGTATGG 70 0.0 36.02808 6 CGTATCT 20 0.0020948674 36.02808 6 CGTATCA 60 1.8189894E-12 36.02808 6 CGTATAG 85 0.0 36.02808 6 CGTATAA 35 6.8976806E-7 36.02808 6 CGACGTG 30 9.934616E-6 36.02808 3 CGACGTC 25 1.4384423E-4 36.02808 3 ACGTTTA 240 0.0 36.02808 5 TTTACGG 25 1.4384423E-4 36.02808 8 >>END_MODULE