FastQCFastQC Report
Thu 26 May 2016
SRR2099165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50599
Sequences flagged as poor quality0
Sequence length42
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTACGTCGGGAGACGCAGCACTTTGACCCAGTCCGTGTCG3990.7885531334611355No Hit
GACTACGTCATGACGATCGGAGGACTACGTTTTTTTTTTTTT3020.596849740113441No Hit
CACTACGATGACGGGCAGCGCGTGATAGCAGCGATGCGGCTC3000.5928970928279215No Hit
AACTACGTAACTGCCAGTGGTACCCAGTACCTCTTGTATATA2890.5711575327575644No Hit
GACTACGTAGTGACAGGCGCGGTGGCTCACGCCTGTAATCCC2270.4486254669064606No Hit
GGCAACGTATTCGGGCACAGAAACCACGCGGGTTGGCGAGCA1960.3873594339809087No Hit
GAGGACGTGTGGGAACAGGGACTGTGTCTGCTTCTAGATGCA1790.35376193205399314No Hit
GACTACAAACGAGAAGGCCAGGAAGAGAAGCCAGAATATTTA1610.3181881064843179No Hit
CACTACGATGACGGGGCAGCGCGTGATAGCAGCGATGCGGCT1580.3122591355560387No Hit
GTCTACGAACATGTTGCGGTATGCTTCGTCGTTGCTCCATTC1280.2529694262732465No Hit
GACTACGTTCCGACTACGTTTTTTTTTTTTTTTTTTTTTTTT1240.24506413170220756No Hit
GGCAACGTATTCGGCACAGAAACCACGCGGGTTGGCGAGCAG1210.23913516077392832No Hit
GACTACGTGGACGGCGCAACTTGAGCTTCAGTTGCCATTGCA1040.2055376588470128No Hit
GACTACGTCCCGGAGATGGCTCAGTGGGTAAGAGCACCCGAC990.19565604063321412No Hit
GAGGACGTGTGGAGAACAGGGACTGTGTCTGCTTCTAGATGC930.18379809877665565No Hit
GACTACGTAGGAGATAAAGGTCTTTGCAGTGATGACAGAAAG880.17391648056285697No Hit
GACTACGCGCAATCCGACGATCTTAGGACTCTGAACCCCTAT870.17194015692009723No Hit
GACTACGTTGCCGATCAGTCGGTACTCCCTGGCATCTCACTT870.17194015692009723No Hit
GACTACGTGGTGGTTTAATAGTAAAAGACTGGTTAATGATAA870.17194015692009723No Hit
GACTACGTGTCTAAAACAGTCCCTCTGCCCTGGACCCCCGGC850.16798750963457776No Hit
AACTACGTGGAGCAGTCCGACGATCACAAGACTACGTTTTTT800.15810589142077905No Hit
AACTACGTAACTGGCCAGTGGTACCCAGTACCTCTTGTATAT770.15217692049249984No Hit
GACTACGTAGGAATAAAGGTCTTTGCAGTGATGACAGAAAGA760.1502005968497401No Hit
GACTACGTACGCCGCTGTACGTCAACGACTTCAACACCCGTG730.1442716259214609No Hit
GACTACGTGGTAAGTCACTCAAAAGTAAGTGAGTGACTTAGG700.13834265499318169No Hit
GACTACGTTCATGAGGACGGTTCCTAGATCAACAGTATTCCT680.1343900077076622No Hit
GAGGACGTGTGGAGCTTCTTGAGAACAGGGACTGTGTCTGCT660.13043736042214274No Hit
GTCTACGAACATGGTTGCGGTATGCTTCGTCGTTGCTCCATT650.128461036779383No Hit
GGCAACGTATTCCGCGTGGCACAGAAACCACGCGGGTTGGCG640.12648471313662324No Hit
TGCTACGTCGCTGGGAAACGGGGCGCGGCTGGAAAGGCGGCC600.1185794185655843No Hit
GACTACGTGGTACCTAAGTCACTCAAAAGTAAGTGAGTGACT580.11462677128006482No Hit
GACTACGTCCATGGGCGGGGCCCGCGTGCCCGTTCGTTCGCT550.10869780035178561No Hit
GACTACGTGCGCCAGTCCGACGATCGTAGGACTGTAGAACTC520.1027688294235064Illumina DpnII expression Sequencing Primer (95% over 21bp)
GACTACGTTGTGAGGAAAAAGAATGCCAAAATCAGCAGCCTC520.1027688294235064No Hit
GACTACGTGCTGGAGAGCCCGCTGCCTGCCGGCTCCCTGCCT520.1027688294235064No Hit
TACTACGCAGCTCAGTCCGACGATCAGAAAAGAAAAAAAAAA510.10079250578074665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAT309.841526E-636.0000046
CGTTCAC356.80473E-736.0000046
CGTCACG601.8189894E-1236.0000046
CGATAAT453.2887328E-936.0000046
TCTAGGT309.841526E-636.0000042
ACGTCTC356.80473E-736.0000045
ACGTTTC309.841526E-636.0000045
ACGTCCC356.80473E-736.0000045
ACGTTCC309.841526E-636.0000045
ACGTTAA356.80473E-736.0000045
ACGTAGA1200.036.0000045
CGTAGTG453.2887328E-936.0000046
CGTAGTA309.841526E-636.0000046
ACGTAAG601.8189894E-1236.0000045
ACGTAAA453.2887328E-936.0000045
CGTAGGG309.841526E-636.0000046
AGGTGTC309.841526E-636.0000045
GCTACGT700.036.0000042
CGTGGAG356.80473E-736.0000046
GACGTGT601.8189894E-1236.0000044
Produced by FastQC (version 0.11.5)