##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2099165_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50599
Sequences flagged as poor quality	0
Sequence length	42
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.722800845866516	37.0	37.0	37.0	35.0	37.0
2	34.835925611178084	35.0	35.0	37.0	32.0	37.0
3	35.68380798039487	37.0	35.0	37.0	35.0	37.0
4	35.67617937113382	37.0	35.0	37.0	35.0	37.0
5	36.15150497045396	37.0	35.0	39.0	32.0	39.0
6	37.50299413031878	39.0	37.0	39.0	35.0	39.0
7	37.45801300420957	39.0	37.0	39.0	35.0	39.0
8	36.94774600288543	39.0	37.0	39.0	34.0	39.0
9	31.955097926836498	33.0	31.0	34.0	30.0	34.0
10	32.194885274412535	34.0	31.0	34.0	30.0	34.0
11	32.14425186268503	34.0	31.0	34.0	30.0	34.0
12	35.64111938971126	37.0	35.0	37.0	35.0	37.0
13	31.409355916124824	31.0	31.0	34.0	30.0	34.0
14	31.327852329097414	31.0	31.0	34.0	28.0	34.0
15	30.97227217929208	31.0	30.0	34.0	27.0	34.0
16	33.49441688570921	35.0	33.0	37.0	26.0	37.0
17	34.408427044012726	35.0	35.0	37.0	30.0	37.0
18	34.2746299333979	35.0	35.0	37.0	30.0	37.0
19	33.71592323958971	35.0	33.0	37.0	28.0	37.0
20	33.89096622462895	35.0	33.0	37.0	28.0	37.0
21	35.503705606830174	37.0	34.0	39.0	28.0	39.0
22	35.92043321014249	38.0	35.0	39.0	30.0	39.0
23	36.38459258088105	38.0	35.0	39.0	32.0	39.0
24	36.684262534832705	38.0	35.0	39.0	32.0	39.0
25	36.81991738967173	39.0	35.0	39.0	33.0	39.0
26	38.28915591217218	40.0	37.0	41.0	34.0	41.0
27	38.4411352003004	40.0	38.0	41.0	34.0	41.0
28	38.63374770252376	40.0	38.0	41.0	35.0	41.0
29	38.76843415877784	40.0	38.0	41.0	36.0	41.0
30	38.81274333484851	40.0	38.0	41.0	36.0	41.0
31	38.91203383466076	40.0	38.0	41.0	36.0	41.0
32	38.98970335382122	40.0	38.0	41.0	36.0	41.0
33	38.998399177849365	40.0	38.0	41.0	36.0	41.0
34	38.99873515286863	40.0	38.0	41.0	36.0	41.0
35	38.944287436510606	40.0	38.0	41.0	36.0	41.0
36	38.92205379552956	40.0	38.0	41.0	36.0	41.0
37	38.83149864621831	40.0	38.0	41.0	35.0	41.0
38	38.72323563706792	40.0	38.0	41.0	35.0	41.0
39	38.69463823395719	40.0	38.0	41.0	35.0	41.0
40	38.75114132690369	40.0	38.0	41.0	35.0	41.0
41	38.74353248087907	40.0	38.0	41.0	35.0	41.0
42	38.738888120318585	40.0	38.0	41.0	35.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3521514587025223
1101	2	0.5857064346177125
1101	3	0.23070278843775327
1101	4	0.37392419418897305
1101	5	0.5393183856678618
1101	6	0.3599454005145759
1101	7	0.32818320674684287
1101	8	-0.2516300518987933
1101	9	0.5181764078077364
1101	10	0.3687788569649513
1101	11	0.4622059637256015
1101	12	0.09684621194529797
1101	13	0.0605542772085883
1101	14	0.11660714947451822
1101	15	-0.07942910861194719
1101	16	-0.33173311566760333
1101	17	0.003727664944001674
1101	18	0.01879186798778676
1101	19	-0.2252464196049644
1101	20	-0.36599384316861716
1101	21	-0.6617833276713725
1101	22	-1.2207959040184022
1101	23	-0.4600872816364827
1101	24	-0.4417724759449442
1101	25	-0.5335709081524982
1101	26	-0.35687169282094544
1101	27	-0.4776710386021179
1101	28	-0.15550571898134535
1101	29	-0.02025018117380739
1101	30	-0.33878659305348435
1101	31	-0.3302551573943191
1101	32	-0.26734248993404464
1101	33	-0.45777868796702137
1101	34	-0.4951993732595241
1101	35	-0.4538970631856927
1101	36	-0.16377036975653425
1101	37	-0.07935671201271788
1101	38	-0.4739265893350435
1101	39	-0.6387422744305482
1101	40	-0.43890638777309476
1101	41	-0.17519398636089534
1101	42	-0.08050048525201703
1102	1	0.33965145870251945
1102	2	0.18668046059173804
1102	3	0.38118980142476744
1102	4	0.4797683500331331
1102	5	0.5386690350185148
1102	6	0.5219583875275617
1102	7	0.5801312586948981
1102	8	0.7641167013479659
1102	9	0.04220238183370739
1102	10	-0.09128607809998357
1102	11	0.3427254442450831
1102	12	-0.470361580262491
1102	13	-0.0652574111031008
1102	14	-0.23095778559041946
1102	15	-0.29550053718337566
1102	16	0.035312338877851346
1102	17	-1.0355580493417165
1102	18	-1.2711431969472784
1102	19	-1.1687529131114616
1102	20	-1.0708639730387475
1102	21	-0.0731469640350042
1102	22	-0.7293998001222946
1102	23	0.14445817290896912
1102	24	0.1725132383407697
1102	25	-0.13714233672392595
1102	26	-0.3622288356780885
1102	27	-0.5460151944462766
1102	28	-0.7753109137865408
1102	29	-0.005477453901079343
1102	30	-0.09803983980673081
1102	31	0.18516692052775596
1102	32	0.08363153603998086
1102	33	-0.2574540126423486
1102	34	-0.26987469793485275
1102	35	-0.008767193055817302
1102	36	-0.07204959053575521
1102	37	0.051812119156110725
1102	38	-0.14048503089348685
1102	39	-0.025430586118865506
1102	40	-0.07754275140946021
1102	41	0.31474107857416556
1102	42	0.16609042383888806
1103	1	-0.027995600121009545
1103	2	0.49894172873536036
1103	3	0.4858498472612851
1103	4	0.2062771353654469
1103	5	0.4334360327266893
1103	6	0.40259245933810206
1103	7	0.6458302655703747
1103	8	0.6432228892776806
1103	9	0.7740587607489076
1103	10	0.8290729746120107
1103	11	0.553382434313832
1103	12	0.26302268253353134
1103	13	-0.2592986639678827
1103	14	-0.3253046152313672
1103	15	-0.4110467556707711
1103	16	-1.772174292138189
1103	17	-0.5418605703501171
1103	18	-1.4709140143651567
1103	19	-1.0134817137226122
1103	20	-1.058640901992149
1103	21	-1.819136268847842
1103	22	-0.5980017863713414
1103	23	-1.015969634577658
1103	24	-1.1153018877096486
1103	25	-1.1791591434466113
1103	26	-0.9399599281150586
1103	27	-0.3460533915432933
1103	28	-0.13932924839311056
1103	29	0.046661583532078055
1103	30	-0.013051298935842226
1103	31	0.3719507249586229
1103	32	0.042951627713016194
1103	33	0.1701624885035642
1103	34	0.3636241561522411
1103	35	0.4005147015201942
1103	36	0.1928472773022918
1103	37	0.29417269975198224
1103	38	0.3451910577237811
1103	39	0.345081254981217
1103	40	0.40374067105043565
1103	41	0.5292177783449858
1103	42	0.6393524559244526
1104	1	-0.37463425558319585
1104	2	0.2126544865657678
1104	3	0.030540450775419004
1104	4	-0.49425762399284423
1104	5	0.21399371034318904
1104	6	0.1193609849301609
1104	7	-0.7185700400063979
1104	8	-0.23588329865203406
1104	9	-0.5422132025818769
1104	10	0.07754509073118498
1104	11	-0.35857325705362086
1104	12	-0.06776417766509013
1104	13	-0.1431794890251794
1104	14	-0.3088798635124945
1104	15	-0.16563040731324818
1104	16	-0.0815707780052648
1104	17	-0.8277658415495068
1104	18	-0.5698444956485815
1104	19	-0.07784382220236807
1104	20	-0.39553929771407326
1104	21	-0.8523677432557832
1104	22	-0.4177114884339872
1104	23	-0.5438535154027164
1104	24	0.04264310847064223
1104	25	-0.20207740165898969
1104	26	-0.3752158486650998
1104	27	-0.16939181782289836
1104	28	0.14676700829138412
1104	29	0.020496572072950414
1104	30	-0.11102685279374924
1104	31	0.41893315429399536
1104	32	0.21350166591011543
1104	33	0.08020832501998854
1104	34	0.054800626740473035
1104	35	0.1340899498013215
1104	36	0.38249586400969804
1104	37	0.4154484827924776
1104	38	0.1192552288467752
1104	39	0.06547850479022799
1104	40	0.3120676382009293
1104	41	0.15889692273000833
1104	42	-0.015727757979291823
1105	1	-0.22445110540004265
1105	2	0.3786551525664308
1105	3	0.1630745833095517
1105	4	-0.4581270878623087
1105	5	0.18419018053965885
1105	6	-0.041977676408507136
1105	7	0.10510628366992592
1105	8	-0.39938646215519924
1105	9	0.1579199975513248
1105	10	0.3565993697854637
1105	11	0.0545136560332935
1105	12	0.014153904252992788
1105	13	-0.017971363817053998
1105	14	0.0630815084488745
1105	15	0.09460935292651129
1105	16	0.3243566279221426
1105	17	-0.18024669403035887
1105	18	-0.32159274739682786
1105	19	0.26065101629247067
1105	20	0.6359292337544602
1105	21	0.39655000566196463
1105	22	-0.21791128863378617
1105	23	0.010425538876333462
1105	24	0.4966890625165945
1105	25	0.07348037389878925
1105	26	0.0498590764098239
1105	27	-0.05683770526878362
1105	28	0.11180197332635089
1105	29	-0.28403223245585707
1105	30	-0.1333378751047718
1105	31	-0.341473106112268
1105	32	-0.1218296694212242
1105	33	0.10568285049451731
1105	34	-0.12468655274670937
1105	35	-0.32761501190363873
1105	36	-0.09486011334627875
1105	37	-0.21525414791015862
1105	38	-0.8655932560017092
1105	39	-0.7522038128920912
1105	40	-0.572880746747451
1105	41	-0.673270909437818
1105	42	-0.29428253653406955
1106	1	0.11742923648029802
1106	2	-0.33312072587611397
1106	3	0.17067192424022437
1106	4	0.12639332999144415
1106	5	-0.17241000939386453
1106	6	-0.05764719207802216
1106	7	-0.0026809907840217306
1106	8	0.010869948101209559
1106	9	0.48792949422748677
1106	10	0.4363714495575479
1106	11	0.02269979088609375
1106	12	0.44530300206875495
1106	13	-0.265834611680301
1106	14	-0.6168805048464705
1106	15	-1.3237192320687399
1106	16	-1.3328133625811844
1106	17	-1.0620130757967416
1106	18	-0.9346031937406067
1106	19	-1.2678390121975625
1106	20	-0.8916111271192406
1106	21	-0.44912900668371947
1106	22	-1.3271230645122287
1106	23	-1.1989761705253699
1106	24	-1.20843914261161
1106	25	-0.9431388093870652
1106	26	-1.0218408286234109
1106	27	-0.7358500509477963
1106	28	-0.48174028688257664
1106	29	-0.43645388487750836
1106	30	-0.34511375354731655
1106	31	-0.2507798487523445
1106	32	-0.3003671812920672
1106	33	-0.35253177438677596
1106	34	-0.4266808547410079
1106	35	-0.5949464459017406
1106	36	-0.3697888882750533
1106	37	-0.07565300830901833
1106	38	-0.19275374982887428
1106	39	0.01465278729784103
1106	40	0.1749825011157924
1106	41	0.01523811240453199
1106	42	-0.13806221364708193
1107	1	-0.839835720784663
1107	2	-1.1213448474335692
1107	3	-0.6317972115622439
1107	4	-0.5350501647853818
1107	5	-0.72606622971675
1107	6	-0.8624904969213247
1107	7	-0.9846373060736653
1107	8	-0.8993864621551992
1107	9	-0.7010543614230365
1107	10	-0.5921185789324817
1107	11	-0.67625557473594
1107	12	-0.5243076342085473
1107	13	0.1230542772085883
1107	14	0.3451327905001591
1107	15	0.36384012215728134
1107	16	0.4012797048452157
1107	17	-0.32127233505600117
1107	18	-0.16774659355067456
1107	19	-0.12396436832291613
1107	20	-0.9153528175275909
1107	21	-0.21883460972265567
1107	22	-0.29483436555685927
1107	23	0.023246051696851566
1107	24	0.0223300881576165
1107	25	-0.04190424148583105
1107	26	0.075500102050853
1107	27	-0.08247873090981273
1107	28	0.2143660758904531
1107	29	0.15186520344158083
1107	30	0.1743544325875419
1107	31	0.5046807400415787
1107	32	0.057657510065958206
1107	33	0.3620931069047728
1107	34	0.24710831904816644
1107	35	0.05700037271174807
1107	36	-0.12050113898730075
1107	37	0.5026945700385639
1107	38	0.04466315425469958
1107	39	0.14523208454380665
1107	40	-0.35493202879873564
1107	41	-0.03224526841218278
1107	42	0.12879438654285025
1108	1	0.09606171511277495
1108	2	0.4042961782074599
1108	3	0.4066643268992962
1108	4	0.05469342495820939
1108	5	0.33803633438581215
1108	6	0.34263770820687967
1108	7	0.20767038623402811
1108	8	0.023690460921720558
1108	9	0.3245866642179891
1108	10	0.2412147544008505
1108	11	0.0673341688538045
1108	12	0.07825646835555489
1108	13	0.28972094387525615
1108	14	-0.0523031069357387
1108	15	0.0817888401060003
1108	16	-0.5089767054111931
1108	17	0.20436869058502793
1108	18	-0.20620813201221466
1108	19	0.37603563167708387
1108	20	0.3154164132416355
1108	21	0.9221910313029866
1108	22	0.7051656344431407
1108	23	0.40786143631223126
1108	24	0.5095095753371055
1108	25	-0.15728885687044425
1108	26	-0.0014229748722272006
1108	27	0.007264858833778476
1108	28	-0.0035826420582623086
1108	29	0.29289084446722313
1108	30	0.007687765920870504
1108	31	0.0944243297851699
1108	32	0.5832985357069873
1108	33	-0.06098381617215409
1108	34	0.27274934468918843
1108	35	0.44161575732712777
1108	36	0.5333450148588526
1108	37	0.579617646961637
1108	38	-0.09636248677094272
1108	39	-0.02143458212285765
1108	40	0.27327309940639566
1108	41	-0.006604242771153679
1108	42	-0.33274407499560965
1109	1	-0.780348541297478
1109	2	-0.29262689871561776
1109	3	-0.7117972115622422
1109	4	-0.8894091391443553
1109	5	-0.5640149476654699
1109	6	-0.4517212661520915
1109	7	-0.6851501265864854
1109	8	-1.3491300518987899
1109	9	-0.26515692552559855
1109	10	-0.5828878097017132
1109	11	-0.17779403627439905
1109	12	-0.27815378805470203
1109	13	-0.07027905612474328
1109	14	0.3041071494745182
1109	15	-0.1869291086119489
1109	16	0.3274335509990678
1109	17	-0.014605668389336302
1109	18	0.7404585346544508
1109	19	0.12475358039502993
1109	20	0.08567282349804373
1109	21	0.41655000566196065
1109	22	0.43337076264826635
1109	23	0.049912718363515296
1109	24	-0.26177247594494446
1109	25	-0.18857090815249222
1109	26	-0.15270502615427262
1109	27	-0.19017103860211648
1109	28	-0.28717238564800596
1109	29	0.0805831521595266
1109	30	0.10871340694651366
1109	31	0.16057817593901547
1109	32	0.3243241767326239
1109	33	0.013887978699642645
1109	34	-0.23853270659286352
1109	35	-0.2788970631856884
1109	36	-0.024603703089866258
1109	37	-0.1793567120127193
1109	38	-0.032259922668380625
1109	39	-0.6070756077638819
1109	40	-0.4339063877730922
1109	41	-0.47019398636089704
1109	42	-0.18300048525201618
1110	1	-1.4277904017626
1110	2	-0.3858051932892579
1110	3	-0.5038902348180585
1110	4	-1.2225874337179974
1110	5	-0.6920769631693489
1110	6	-0.546449948322639
1110	7	-0.6927470258112933
1110	8	-1.0356416798057637
1110	9	-0.32717242940156765
1110	10	-0.6678490500117924
1110	11	-0.38198008278602913
1110	12	-1.0037351834035348
1110	13	-0.12113176930304093
1110	14	-0.13310215285106608
1110	15	-0.23902213186776322
1110	16	-0.779698231946675
1110	17	0.08570440913004518
1110	18	-0.22946394596570485
1110	19	-1.151990605651477
1110	20	-0.5921566338662956
1110	21	-1.1460856532527686
1110	22	-0.019342415646306677
1110	23	0.3061917881309597
1110	24	-0.809214336410065
1110	25	-0.4876406755943563
1110	26	-0.09146471607675721
1110	27	-0.0413338292997949
1110	28	-0.12701734688831579
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2210	3	-0.28179721156224247
2210	4	-0.03857580581102127
2210	5	0.1518183856678661
2210	6	0.1974454005145745
2210	7	0.39068320674684287
2210	8	0.28586994810120814
2210	9	0.18067640780773075
2210	10	0.26877885696494985
2210	11	0.3872059637255987
2210	12	0.12184621194529655
2210	13	-0.3144457227914117
2210	14	-0.3708928505254825
2210	15	-0.16692910861194932
2210	16	-0.01923311566760333
2210	17	-0.04627233505600259
2210	18	0.5187918679877868
2210	19	0.47475358039503135
2210	20	-0.30349384316861716
2210	21	0.06321667232862893
2210	22	-0.17079590401839795
2210	23	0.11491271836351302
2210	24	0.3707275240550558
2210	25	0.44142909184750323
2210	26	0.743128307179056
2210	27	-0.052671038602120746
2210	28	0.49449428101866033
2210	29	0.3797498188261912
2210	30	-0.12628659305348577
2210	31	-0.10525515739431768
2210	32	-0.3173424899340418
2210	33	-0.33277868796702137
2210	34	-0.4576993732595298
2210	35	-0.09139706318568841
2210	36	-0.1512703697565314
2210	37	-0.4293567120127193
2210	38	-0.23642658933504634
2210	39	-0.013742274430548207
2210	40	-0.18890638777309476
2210	41	0.12480601363910182
2210	42	-0.2555004852520213
2211	1	0.14452950748300708
2211	2	0.35582838583722776
2211	3	0.4535686420962932
2211	4	0.47026565760361194
2211	5	0.5600500929849375
2211	6	0.12031125417311017
2211	7	0.45501247503953124
2211	8	0.8035528749304746
2211	9	0.0681764078077336
2211	10	0.28280324720885375
2211	11	0.2402547442134022
2211	12	0.6145291387745644
2211	13	0.24500549672078265
2211	14	0.5943510519135415
2211	15	0.6696562572417086
2211	16	-0.01679409127735454
2211	17	0.9714105917732638
2211	18	0.41574308749998323
2211	19	0.5686560194194215
2211	20	-0.16050603829057053
2211	21	0.08760691623106709
2211	22	0.3252406813474522
2211	23	0.14113223055863955
2211	24	-0.10274808570104454
2211	25	-0.15979042034761903
2211	26	-0.02424974160143023
2211	27	0.3293411565198312
2211	28	0.5536406224820709
2211	29	0.36481079443594666
2211	30	-0.009823178419338774
2211	31	0.3267570377276314
2211	32	0.44790141250498294
2211	33	0.28673350715492774
2211	34	0.22553233405754725
2211	35	0.6211029368143102
2211	36	0.5023881668288297
2211	37	0.1840579221336185
2211	38	0.41082950822593034
2211	39	0.7008918719109118
2211	40	0.5787765390561717
2211	41	0.8107816233952008
2211	42	0.6350482952357837
2212	1	-1.0467121776611137
2212	2	-0.3733844744731911
2212	3	-0.1658881206531504
2212	4	0.09665146691624926
2212	5	-0.00840888705941012
2212	6	-0.10141823584906007
2212	7	-0.19908952052588091
2212	8	-0.45503914280788393
2212	9	-0.5113690467377197
2212	10	-0.792584779398684
2212	11	-0.33097585445621647
2212	12	-0.3031537880547006
2212	13	0.10032700448131493
2212	14	-0.31862012325275657
2212	15	-0.21920183588467523
2212	16	-0.37377857021305516
2212	17	0.14463675585309232
2212	18	0.3392464134423321
2212	19	0.3361172167586659
2212	20	-0.39553929771407326
2212	21	-0.33451060039864444
2212	22	-0.3673868131093059
2212	23	-0.07145091800011727
2212	24	0.003682069509601149
2212	25	-0.21993454451612848
2212	26	-0.003462601911849106
2212	27	0.15528350685242742
2212	28	-0.3191420826177094
2212	29	-0.48275018117380597
2212	30	-0.3512865930534872
2212	31	-0.06320970284886585
2212	32	8.393282477783259E-4
2212	33	0.36267585748752396
2212	34	0.07752789946774641
2212	35	-0.09480615409478332
2212	36	0.04645690297073912
2212	37	0.1979160152600059
2212	38	0.07380068339222845
2212	39	-0.1341968198850978
2212	40	-0.2025427514094602
2212	41	-0.5229212590881716
2212	42	-0.8339095761611119
2213	1	0.18965145870252087
2213	2	0.11070643461771823
2213	3	0.29320278843775327
2213	4	0.24892419418897305
2213	5	0.026818385667866096
2213	6	-0.2400545994854255
2213	7	-0.059316793253152866
2213	8	-0.42663005189879044
2213	9	0.1931764078077336
2213	10	-0.04372114303505015
2213	11	-0.0127940362743999
2213	12	0.20934621194530223
2213	13	0.023054277208586882
2213	14	0.1291071494745175
2213	15	0.1830708913880521
2213	16	-0.15673311566759907
2213	17	-0.08377233505600401
2213	18	0.5187918679877868
2213	19	0.16225358039503135
2213	20	-0.015993843168622845
2213	21	-0.12428332767137107
2213	22	-0.2207959040184022
2213	23	-0.47258728163648556
2213	24	-0.6667724759449456
2213	25	-0.20857090815249535
2213	26	-0.33187169282093976
2213	27	-0.2651710386021193
2213	28	-0.21800571898134535
2213	29	-0.3327501811738074
2213	30	-0.013786593053488616
2213	31	0.20724484260568232
2213	32	-0.0673424899340418
2213	33	0.017221312032980052
2213	34	0.2548006267404759
2213	35	-0.05389706318568699
2213	36	-0.2137703697565314
2213	37	0.09564328798727928
2213	38	0.3760734106649579
2213	39	0.2987577255694518
2213	40	0.5610936122269052
2213	41	0.4998060136391018
2213	42	0.05699951474797871
2214	1	-0.47430202966957324
2214	2	-0.3044098444520529
2214	3	-0.3759832580738731
2214	4	-0.5365409220900972
2214	5	-1.854867660843766
2214	6	-1.3604034366947317
2214	7	-0.7276307467415251
2214	8	-1.861223075154605
2214	9	-0.47833522009924323
2214	10	-0.40040718954667653
2214	11	-0.4866312455767243
2214	12	-0.10838634619423715
2214	13	0.006775207441148012
2214	14	-0.016823083083622237
2214	15	0.3074894960392136
2214	16	-0.3378377668303898
2214	17	0.46942533936260133
2214	18	-0.25271975991918794
2214	19	0.42940474318572797
2214	20	0.4310991800871946
2214	21	1.005077137444907
2214	22	0.4690296773769518
2214	23	0.2247964392937476
2214	24	0.3186926403341275
2214	25	0.4425918825451802
2214	26	0.013186446713937983
2214	27	0.5633173334909074
2214	28	0.4427500949721477
2214	29	0.3835288885936379
2214	30	0.3580157325279103
2214	31	-0.13694120390594833
2214	32	-0.4190866759805516
2214	33	0.1718724748236724
2214	34	0.2059634174381486
2214	35	0.4234285182096613
2214	36	-0.014061067430951368
2214	37	0.12762003217332563
2214	38	0.010375736246352574
2214	39	0.18160656277875376
2214	40	0.06516337966876762
2214	41	0.24980601363910182
2214	42	0.3500227705619352
2215	1	-0.45552926418904605
2215	2	-0.4296550111654156
2215	3	-0.354688777827306
2215	4	-0.2905336371363276
2215	5	-2.3395972769827367
2215	6	-1.284331707919165
2215	7	-0.24290113060255436
2215	8	-0.278286678404811
2215	9	-1.2571247970115422
2215	10	-0.9152572876133611
2215	11	-1.0534566868768103
2215	12	-0.9311658362474731
2215	13	0.08088560250979171
2215	14	0.378805944655241
2215	15	0.32584197572539964
2215	16	-0.4915222722941053
2215	17	0.1365589902452058
2215	18	0.721502711361282
2215	19	0.1440306888287708
2215	20	-0.02533119256621319
2215	21	0.8403251060635668
2215	22	0.47995710802979374
2215	23	-0.14141258284130487
2215	24	0.08473354815144063
2215	25	0.231188127992084
2215	26	-0.026600608483590804
2215	27	0.139949443325591
2215	28	-0.06243342982471489
2215	29	-0.5158827112942888
2215	30	-0.48984080992095613
2215	31	-0.2903455188401054
2215	32	-0.4724629718617521
2215	33	-0.48699555543690565
2215	34	-0.3909825057896441
2215	35	-0.9752826053543657
2215	36	-0.3934390444553273
2215	37	-0.40586273610910695
2215	38	-0.13266152909407936
2215	39	0.0332456773766765
2215	40	0.10537072066064468
2215	41	-0.0634469984090913
2215	42	-0.17673542501105288
2216	1	0.07083425440144708
2216	2	0.3650075098865315
2216	3	-0.09416280296009205
2216	4	-0.2889790316174725
2216	5	-0.27197193691278443
2216	6	-0.23924814787252302
2216	7	-0.18149421260799414
2216	8	-0.13966768630739068
2216	9	-0.5447268179987184
2216	10	-0.37127490647590733
2216	11	-0.43886930509160393
2216	12	-0.44159464826975636
2216	13	-0.06511776580216377
2216	14	-0.03782833439645117
2216	15	0.3911354075170834
2216	16	0.5054980671280944
2216	17	0.7755018584923832
2216	18	0.8368026206759609
2216	19	1.1527105696423448
2216	20	0.32782336113245236
2216	21	0.6707435540490607
2216	22	0.5538008701751522
2216	23	0.35657938503018016
2216	24	0.11414150254967836
2216	25	0.18390221012707286
2216	26	0.4150369093295936
2216	27	0.08294724096777628
2216	28	-0.07512937489531879
2216	29	-0.28113727794799814
2216	30	-0.0018242274620874355
2216	31	-0.1723250498674389
2216	32	-0.21115969423511416
2216	33	-0.08331632237562303
2216	34	-0.16025313670039054
2216	35	0.06733949595409428
2216	36	-0.43191553104685454
2216	37	-0.3492491851310007
2216	38	-0.6881739011630046
2216	39	-0.38040894109721535
2216	40	-0.38057305443975764
2216	41	-0.4813767820598258
2216	42	-0.16235532396169816
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	2.0
26	12.0
27	40.0
28	115.0
29	226.0
30	453.0
31	842.0
32	1450.0
33	2344.0
34	4357.0
35	7882.0
36	11684.0
37	10029.0
38	11163.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	92.22316646574042	2.9348406094982114	0.762860926105259	4.0791319986561
2	3.589003735251685	93.99395244965315	1.7174252455582124	0.6996185695369475
3	2.3735646949544456	0.9802565268088302	0.6541631257534734	95.99201565248325
4	1.992134231901816	1.8439099586948358	95.00187750746062	1.162078301942726
5	1.535633819518558	96.3773271670817	1.6561919443456263	0.4308470690541128
6	0.8656297555287654	0.6541631257534734	0.9624696140239926	97.51773750469377
7	93.13213961026128	5.409304715601407	0.7984505316415669	0.6601051424957508
8	1.073143738018538	5.6344987055080145	92.27652720409493	1.0158303523785055
9	30.990731042115456	27.036107432953223	23.204015889642086	18.769145635289235
10	35.75564734480919	19.05373623984664	25.708017945018675	19.482598470325502
11	31.161310723744023	25.291513498557254	23.204474485157515	20.342701292541207
12	31.281250617601135	22.30083598490089	22.65262159331212	23.765291804185853
13	41.755114141713605	26.72398458345686	12.188951477418717	19.33194979741081
14	28.771888216925568	29.95968220087751	21.755800624530615	19.512628957666312
15	39.39879046602633	26.56231471599668	17.150875528677023	16.888019289299972
16	36.29123105199707	27.03017846202494	20.310678076641832	16.367912409336153
17	18.463180362860193	25.24803351911143	15.824736155579272	40.46404996244911
18	18.343546155366674	24.21822494564143	14.182644791460763	43.25558410753113
19	29.749975294001384	23.777053068485028	22.761142405376024	23.711829232137564
20	26.336488863416275	28.810846064151463	21.468803731299037	23.383861341133226
21	22.065653471412478	24.22577521294887	23.04788632186407	30.660684993774577
22	28.98278622107156	24.875985691416826	22.296883337615366	23.844344749896244
23	24.86017510227475	29.53022787011601	24.4846736101504	21.124923417458845
24	21.498448585940434	25.842407952726337	29.7515761181051	22.907567343228127
25	22.373959959683	22.911519990513646	26.785114330322735	27.92940571948062
26	24.885867309630623	25.071641732050043	26.741635210182018	23.300855748137312
27	26.765351093895134	25.107215557619718	26.996580960098026	21.130852388387122
28	24.496531552006957	27.006462578311822	25.01235202276725	23.484653846913968
29	26.168501353781696	25.474811755173025	23.925374019249393	24.431312871795885
30	28.885946362576338	23.964900492104587	25.565722642739974	21.583430502579102
31	25.785094567086308	29.61125713946916	23.64671238562027	20.956935907824263
32	21.992529496630368	25.03211525919485	26.241625328563806	26.733729915610983
33	22.902090991738806	23.247954464603342	30.762085457923238	23.087869085734614
34	26.733729915610983	25.399711456748157	28.68238502737208	19.18417360026878
35	24.285064922231665	24.496531552006957	30.162651435799127	21.05575208996225
36	22.59180204751176	28.25210482627772	27.59792877188822	21.558164354322304
37	27.55785687464179	25.644775588450365	25.45307219510267	21.344295341805175
38	23.490582817842252	29.113223581493706	26.237672681278283	21.158520919385758
39	22.355080340731664	31.126351364705418	26.209063778484893	20.30950451607803
40	22.512747539428435	25.22234080398435	26.906201826159137	25.35870983042808
41	20.31463072392735	23.545919879839524	32.32079685369276	23.818652542540367
42	21.46682740765628	25.662562501235204	27.66853099863634	25.202079092472186
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	5.5
9	11.0
10	11.0
11	26.5
12	42.0
13	104.0
14	166.0
15	180.0
16	194.0
17	194.0
18	232.5
19	271.0
20	262.5
21	254.0
22	254.0
23	413.0
24	572.0
25	594.0
26	616.0
27	627.5
28	639.0
29	639.0
30	809.0
31	979.0
32	1012.5
33	1046.0
34	1046.0
35	1163.5
36	1281.0
37	1582.0
38	1883.0
39	2023.5
40	2164.0
41	2164.0
42	2746.5
43	3329.0
44	3298.0
45	3267.0
46	3611.5
47	3956.0
48	3956.0
49	4334.0
50	4712.0
51	4804.0
52	4896.0
53	4896.0
54	5145.5
55	5395.0
56	4698.5
57	4002.0
58	3463.0
59	2924.0
60	2924.0
61	2998.5
62	3073.0
63	2830.0
64	2587.0
65	1752.5
66	918.0
67	918.0
68	722.5
69	527.0
70	509.0
71	491.0
72	491.0
73	353.5
74	216.0
75	151.5
76	87.0
77	74.5
78	62.0
79	62.0
80	49.0
81	36.0
82	19.0
83	2.0
84	2.0
85	1.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.001976323642759738
6	0.0
7	0.001976323642759738
8	0.0
9	0.0
10	0.0
11	0.001976323642759738
12	0.0
13	0.007905294571038952
14	0.001976323642759738
15	0.001976323642759738
16	0.0
17	0.001976323642759738
18	0.017786912784837645
19	0.007905294571038952
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.001976323642759738
34	0.0
35	0.0
36	0.001976323642759738
37	0.0
38	0.0
39	0.003952647285519476
40	0.001976323642759738
41	0.0
42	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
42	50599.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.50663056582145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.222865412445735	19.962845115516117
2	16.01358120895024	11.371766240439534
3	8.566180563286208	9.124686258621711
4	5.566069241901369	7.9052945710389535
5	3.818323499944339	6.7787900946659025
6	2.68284537459646	5.715527974861163
7	1.6197261493932986	4.025771260301587
8	1.296894133363019	3.6838672701041517
9	0.7068907937214739	2.258937923674381
>10	3.3062451296894135	20.609102946698552
>50	0.1280195925637315	3.2510523923397696
>100	0.0723589001447178	5.312357951738177
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCTACGTCGGGAGACGCAGCACTTTGACCCAGTCCGTGTCG	399	0.7885531334611355	No Hit
GACTACGTCATGACGATCGGAGGACTACGTTTTTTTTTTTTT	302	0.596849740113441	No Hit
CACTACGATGACGGGCAGCGCGTGATAGCAGCGATGCGGCTC	300	0.5928970928279215	No Hit
AACTACGTAACTGCCAGTGGTACCCAGTACCTCTTGTATATA	289	0.5711575327575644	No Hit
GACTACGTAGTGACAGGCGCGGTGGCTCACGCCTGTAATCCC	227	0.4486254669064606	No Hit
GGCAACGTATTCGGGCACAGAAACCACGCGGGTTGGCGAGCA	196	0.3873594339809087	No Hit
GAGGACGTGTGGGAACAGGGACTGTGTCTGCTTCTAGATGCA	179	0.35376193205399314	No Hit
GACTACAAACGAGAAGGCCAGGAAGAGAAGCCAGAATATTTA	161	0.3181881064843179	No Hit
CACTACGATGACGGGGCAGCGCGTGATAGCAGCGATGCGGCT	158	0.3122591355560387	No Hit
GTCTACGAACATGTTGCGGTATGCTTCGTCGTTGCTCCATTC	128	0.2529694262732465	No Hit
GACTACGTTCCGACTACGTTTTTTTTTTTTTTTTTTTTTTTT	124	0.24506413170220756	No Hit
GGCAACGTATTCGGCACAGAAACCACGCGGGTTGGCGAGCAG	121	0.23913516077392832	No Hit
GACTACGTGGACGGCGCAACTTGAGCTTCAGTTGCCATTGCA	104	0.2055376588470128	No Hit
GACTACGTCCCGGAGATGGCTCAGTGGGTAAGAGCACCCGAC	99	0.19565604063321412	No Hit
GAGGACGTGTGGAGAACAGGGACTGTGTCTGCTTCTAGATGC	93	0.18379809877665565	No Hit
GACTACGTAGGAGATAAAGGTCTTTGCAGTGATGACAGAAAG	88	0.17391648056285697	No Hit
GACTACGCGCAATCCGACGATCTTAGGACTCTGAACCCCTAT	87	0.17194015692009723	No Hit
GACTACGTTGCCGATCAGTCGGTACTCCCTGGCATCTCACTT	87	0.17194015692009723	No Hit
GACTACGTGGTGGTTTAATAGTAAAAGACTGGTTAATGATAA	87	0.17194015692009723	No Hit
GACTACGTGTCTAAAACAGTCCCTCTGCCCTGGACCCCCGGC	85	0.16798750963457776	No Hit
AACTACGTGGAGCAGTCCGACGATCACAAGACTACGTTTTTT	80	0.15810589142077905	No Hit
AACTACGTAACTGGCCAGTGGTACCCAGTACCTCTTGTATAT	77	0.15217692049249984	No Hit
GACTACGTAGGAATAAAGGTCTTTGCAGTGATGACAGAAAGA	76	0.1502005968497401	No Hit
GACTACGTACGCCGCTGTACGTCAACGACTTCAACACCCGTG	73	0.1442716259214609	No Hit
GACTACGTGGTAAGTCACTCAAAAGTAAGTGAGTGACTTAGG	70	0.13834265499318169	No Hit
GACTACGTTCATGAGGACGGTTCCTAGATCAACAGTATTCCT	68	0.1343900077076622	No Hit
GAGGACGTGTGGAGCTTCTTGAGAACAGGGACTGTGTCTGCT	66	0.13043736042214274	No Hit
GTCTACGAACATGGTTGCGGTATGCTTCGTCGTTGCTCCATT	65	0.128461036779383	No Hit
GGCAACGTATTCCGCGTGGCACAGAAACCACGCGGGTTGGCG	64	0.12648471313662324	No Hit
TGCTACGTCGCTGGGAAACGGGGCGCGGCTGGAAAGGCGGCC	60	0.1185794185655843	No Hit
GACTACGTGGTACCTAAGTCACTCAAAAGTAAGTGAGTGACT	58	0.11462677128006482	No Hit
GACTACGTCCATGGGCGGGGCCCGCGTGCCCGTTCGTTCGCT	55	0.10869780035178561	No Hit
GACTACGTGCGCCAGTCCGACGATCGTAGGACTGTAGAACTC	52	0.1027688294235064	Illumina DpnII expression Sequencing Primer (95% over 21bp)
GACTACGTTGTGAGGAAAAAGAATGCCAAAATCAGCAGCCTC	52	0.1027688294235064	No Hit
GACTACGTGCTGGAGAGCCCGCTGCCTGCCGGCTCCCTGCCT	52	0.1027688294235064	No Hit
TACTACGCAGCTCAGTCCGACGATCAGAAAAGAAAAAAAAAA	51	0.10079250578074665	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.01185794185655843	0.0	0.0
13	0.0	0.0	0.013834265499318169	0.0	0.0
14	0.0	0.0	0.029644854641396073	0.0	0.0
15	0.0	0.0	0.05928970928279215	0.0	0.0
16	0.0	0.0	0.07510029842487005	0.0	0.0
17	0.0	0.0	0.0770766220676298	0.0	0.0
18	0.0	0.0	0.10276882942350639	0.0	0.0
19	0.0	0.0	0.1343900077076622	0.0	0.0
20	0.0	0.0	0.28656692820016205	0.0	0.0
21	0.0	0.0	0.339927666554675	0.0	0.0
22	0.0	0.0	1.5652483250657128	0.0	0.0
23	0.0	0.0	1.9664420245459397	0.0	0.0
24	0.0	0.0	2.2115061562481473	0.0	0.0
25	0.0	0.0	2.2569616000316213	0.0	0.0
26	0.0	0.0	2.3300855748137317	0.0	0.0
27	0.0	0.0	2.365659400383407	0.0	0.0
28	0.0	0.0	2.409138520524121	0.0	0.0
29	0.0	0.0	2.557362793731101	0.0	0.0
30	0.0	0.0	2.861716634716101	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCAT	30	9.841526E-6	36.000004	6
CGTTCAC	35	6.80473E-7	36.000004	6
CGTCACG	60	1.8189894E-12	36.000004	6
CGATAAT	45	3.2887328E-9	36.000004	6
TCTAGGT	30	9.841526E-6	36.000004	2
ACGTCTC	35	6.80473E-7	36.000004	5
ACGTTTC	30	9.841526E-6	36.000004	5
ACGTCCC	35	6.80473E-7	36.000004	5
ACGTTCC	30	9.841526E-6	36.000004	5
ACGTTAA	35	6.80473E-7	36.000004	5
ACGTAGA	120	0.0	36.000004	5
CGTAGTG	45	3.2887328E-9	36.000004	6
CGTAGTA	30	9.841526E-6	36.000004	6
ACGTAAG	60	1.8189894E-12	36.000004	5
ACGTAAA	45	3.2887328E-9	36.000004	5
CGTAGGG	30	9.841526E-6	36.000004	6
AGGTGTC	30	9.841526E-6	36.000004	5
GCTACGT	70	0.0	36.000004	2
CGTGGAG	35	6.80473E-7	36.000004	6
GACGTGT	60	1.8189894E-12	36.000004	4
>>END_MODULE