FastQCFastQC Report
Thu 26 May 2016
SRR2099164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988213
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTACAATCGAACGGCCGACGATCTCAAAACGTAGTCACTGT74590.7547967897609119No Hit
ACGTACAATCGAACCGGCCGACGATCTCAAAACGTAGTCACTG53310.5394585985005257No Hit
ACGTACAATCGAATACGACTCCACTATAGGGAGTCGTATTACC21260.21513580574228428No Hit
ACGTGCATCTCCCCAGTCCGACGATCTTATTACGTGCATTTTT17650.17860521972489737No Hit
ACGTACAATCGAAGGCCGACGATCTCAAAACGTAGTCACTGTT15930.16120006516813684No Hit
ACGTGCATTTGCAGACGATCGCGTTACGTGCATTTTTTTTTTT15030.15209271685355283No Hit
ACGTACAATCGAAGTTGTACGTTTCGAGATCGTCGGACTGTAG14070.14237821198466324No Hit
ACGTGCATACTTTGCGGAGGTGGATTTTCGGGTGAAGGTTTAG12540.12689571984987041No Hit
ACGTACAATCGAAAGTGAGTCGTATTACCGGCCGACGATCTCA12090.12234204569257841No Hit
ACGTGCATGCTCGGCCCCTGAGAGCTTGCTGTTTCTGAGAACA11910.12052057602966162No Hit
ACGTACAATCGAAACCGGCCGACGATCTCAAAACGTAGTCACT11720.1185979136076939No Hit
ACGTGCATCCCTTCCGGCGGCCTCGCCCCAGCCGGAGCCGAGG11300.1143478177275547No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCGA200.001835223437.0270049
TACGCAT251.2282145E-437.0270049
GCACTAG308.280811E-637.0195055
ATACCCG700.037.01957
CGAACGG8950.037.00825510
GGTCGAA355.6204044E-737.00825510
GACCGCT308.298091E-637.00825511
AACTCCG650.037.00825510
GTGCATC199850.036.8379333
ACAATCG59700.036.7094575
GTGCATA184850.036.593383
CGTACAA60450.036.546392
GTGCATG191550.036.4824833
GAACCGG6800.036.46401611
TGCATAC43500.036.4626464
ACGTACA64750.036.4624631
CGAGCTT27450.036.435762
TACAATC60300.036.4018944
GCATCGC14800.036.3941735
ACGAGCT27500.036.3713531
Produced by FastQC (version 0.11.5)