FastQCFastQC Report
Thu 26 May 2016
SRR2099163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221889
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTACAATCGAACGGCCGACGATCTCAAAACGTAGTCACTGT20380.9184772566463412No Hit
ACGTACAATCGAACCGGCCGACGATCTCAAAACGTAGTCACTG14110.6359035373542627No Hit
ACGTACAATCGAATACGACTCCACTATAGGGAGTCGTATTACC5850.26364533618160435No Hit
ACGTGCATTTGCAGACGATCGCGTTACGTGCATTTTTTTTTTT4700.2118176205219727No Hit
ACGTGCATCTCCCCAGTCCGACGATCTTATTACGTGCATTTTT4570.20595883527349262No Hit
ACGTACAATCGAAGGCCGACGATCTCAAAACGTAGTCACTGTT4090.1843263974329507No Hit
ACGTACAATCGAAGTTGTACGTTTCGAGATCGTCGGACTGTAG3420.15413111961386097No Hit
ACGTGCATACTTTGCGGAGGTGGATTTTCGGGTGAAGGTTTAG3400.15322976803717175No Hit
ACGTACAATCGAAAGTGAGTCGTATTACCGGCCGACGATCTCA3360.15142706488379323No Hit
ACGTGCATGCTCGGCCCCTGAGAGCTTGCTGTTTCTGAGAACA3200.1442162522702793No Hit
ACGTACAATCGAAACCGGCCGACGATCTCAAAACGTAGTCACT3130.14106152175186693No Hit
ACGTGCAGTACATCGTGCATTTTTTTTTTTTTTTTTTTTTTTT3080.1388081428101438No Hit
ACGTACAATCGAAAGTCACTGTTGTACGTTTCGAGATCGTCGG2530.11402097445118956No Hit
ACGTGCATGAGGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.10861286499105408No Hit
ACGTACAATCGAAACTGTTGTACGTTTCGAGATCGTCGGACTG2340.10545813447264173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAC355.5978853E-737.00518813
CAGCGCT200.001837471337.00518415
GGGTAGT200.001837471337.00518434
TCGGGGA200.001837471337.00518413
GGAAGTT200.001837471337.00518415
ACGATTC200.001837471337.00518418
CCCGTTT452.5956979E-937.00518432
AGACGAT600.037.00518413
CCCCGTT452.5956979E-937.00518431
AAGCCGC251.2294018E-437.00518415
GACGATT200.001837471337.00518417
CAGACCG251.2294018E-437.00518414
GCATAAG1950.036.9968455
GAAATTA251.2310345E-436.99684511
CGAACGG2150.036.99684510
CGAACCG1850.036.99684510
CATATGT1100.036.9968456
CGAAACT501.7826096E-1036.99684510
ATGGCGT355.6077806E-736.9968457
TAGGTCG251.2310345E-436.9968458
Produced by FastQC (version 0.11.5)