FastQCFastQC Report
Thu 26 May 2016
SRR2099162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592263
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTAGATCGAGGATGCAGTATCGTAGAGATACCTTTCAACC17830.3010486895180013No Hit
GATCCCGCTCGCGAGTCGACGATAATCCCCGGCGGAATGGTCT14930.2520839559452473No Hit
GATCTAGCTGGGGTTGCAATGCCGATGACAACCAGTATCAGCG14930.2520839559452473No Hit
GAACTAACACCCAGATCCAGCATTGCCATGAGCTGTGGTATAG14070.23756337978229264No Hit
GATCTAGCTATAAGTTACAGCAGGGGTGTGTGTGCGTGCGTGC9820.16580471851187734No Hit
GAACTAACACCCAGGATCCAGCATTGCCATGAGCTGTGGTATA9680.16344090378767542No Hit
GATCTAGCTCGGTACGATCTTCGGGATCTAGCTTTTTTTTTTT9060.15297258143763834No Hit
GATCTAGCCTCTGATGGCCTTTATGAGAAGAAAAAGACCTCCC8750.14773842026261982No Hit
GATCTAGCTCGGAGTTAAGACTGTGTTGCTTACTATTGCTATT8560.14453038599406007No Hit
GATCTAGCGAGTTCCGACGATCACGGCGATCTGTAGAACTCTG8390.14166003954324344No Hit
GATCTAGCCGATCCTAAGCCTCCGCTGTCTGTCTGTCTGTCTC6990.11802189230122428No Hit
GATCTAGCCTTCCTGAGCATGTCCAGCATGCACACAGACTTCT6920.11683998493912331No Hit
GATCTAGCACCCTGGCAAAGGCTGCAGGAAAGTATGAGGGAGA6540.11042391640200383No Hit
GATCTAGCGCGGGGCAGGAGAAGGGGGTGGTGTTTTCCCTCCC6140.10367016004714123No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGC251.2268659E-437.030879
TACGCGG600.037.0308659
TAGCGTA9400.037.0277375
TAGCGGC6950.037.0277375
TAGCCCG7200.037.0277375
CCGCAAT308.262585E-637.0277378
TAGTAGT308.262585E-637.0277375
CGCTACG251.2274766E-437.0277338
AGCGCGT1650.037.0246056
GCGGATA600.037.0246057
GCGCTTA850.037.0246057
GTATACG251.2329851E-436.99957712
CCGCATT200.001841317336.99957710
AGGCGCG251.238514E-436.9714618
ACGAGCG200.001848970536.9683429
TAGCGGT10600.036.8530735
TAGCGTT9600.036.834885
TAGCGCG7750.036.7888455
TCTAGCG125600.036.6972433
CTAGCGT37850.036.6791344
Produced by FastQC (version 0.11.5)