FastQCFastQC Report
Thu 26 May 2016
SRR2099161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142060
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCCCGCTCGCGAGTCGACGATAATCCCCGGCGGAATGGTCT4580.3223989863437984No Hit
GATGTAGATCGAGGATGCAGTATCGTAGAGATACCTTTCAACC4560.32099113050823597No Hit
GATCTAGCTGGGGTTGCAATGCCGATGACAACCAGTATCAGCG4300.30268900464592424No Hit
GAACTAACACCCAGATCCAGCATTGCCATGAGCTGTGGTATAG4060.285794734619175No Hit
GATCTAGCCTCTGATGGCCTTTATGAGAAGAAAAAGACCTCCC3250.22877657327889625No Hit
GATCTAGCTATAAGTTACAGCAGGGGTGTGTGTGCGTGCGTGC2480.17457412360974237No Hit
GAACTAACACCCAGGATCCAGCATTGCCATGAGCTGTGGTATA2450.17246233985639872No Hit
GATCTAGCGAGTTCCGACGATCACGGCGATCTGTAGAACTCTG2320.16331127692524286No Hit
GATCTAGCTCGGAGTTAAGACTGTGTTGCTTACTATTGCTATT2200.1548641419118682No Hit
GATCTAGCGATTGGAGACAGCAAAAGAGTAGGGGCTGAGGGTG2110.14852879065183727No Hit
GATCTAGCCGATCCTAAGCCTCCGCTGTCTGTCTGTCTGTCTC1980.1393777277206814No Hit
GATCTAGCACCCTGGCAAAGGCTGCAGGAAAGTATGAGGGAGA1860.13093059270730678No Hit
GATCTAGCTTGTTGTGGCGTCGACCGCTGGCCAGTGCTGTAGT1830.1288188089539631No Hit
GATCTAGCTCCCCGGGATCTAGCTTTTTTTTTTTTTTTTTTTT1680.11825989018724482No Hit
GATCTAGCCTGCGGAAGCCGAGCTGAGTCTGGCCCCGTTCTTG1670.11755596226946362No Hit
GATCTAGCCCTCTGTGGCTTCAAAGAAGCTATTGATTCTGAAG1630.11474025059833874No Hit
GATCTAGCCGATAGGATCCGAGCATCTTATCCACGCTTCCGTT1630.11474025059833874No Hit
GATCTAGCATGCCCAGAGATACCGTGGACACACCAAGGGCCTT1620.11403632268055751No Hit
GATCTAGCTCTACAGGGCCAAAAAGGGCCAAAAAGGGGGAGTG1610.11333239476277628No Hit
GATCTAGCACTCCATGGCTCAGCAGTTAAGAGCACCGACTGTT1580.11122061100943263No Hit
GATCTAGCTCCAAGAAACGTGGGAGGATGTTGGAAGGTTGGAC1540.10840489933830776No Hit
GATCTAGCGCGGGGCAGGAGAAGGGGGTGGTGTTTTCCCTCCC1510.1062931155849641No Hit
GATCTAGCCTTTCGCACTTGCCAAAGGGCTACCCAGCACTGTG1490.10488525974940166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGTCC600.037.02113313
TGACAGA251.2240621E-437.0211313
CTTAACG308.248358E-637.00818
CTCACCG251.2266038E-437.00818
GTCGAGT200.001834536437.008111
TACCAGT200.001834536437.008111
GTCCTAC308.248358E-637.008116
GCGCCCT200.001834536437.008115
TAAGTTA308.248358E-637.008111
CGCGAGT452.579327E-937.008110
TAACGAG200.001834536437.008110
CCACTCG251.2266038E-437.00818
AAGCCGA200.001834536437.008115
CAAGGTG251.2266038E-437.00818
CCTTTCG551.2732926E-1137.00818
GTTCCGA452.579327E-937.008111
CGGTGCG308.248358E-637.00818
CTCGCGC200.001834536437.00818
TTAACGA200.001834536437.00819
AGTTCCG452.579327E-937.008110
Produced by FastQC (version 0.11.5)