FastQCFastQC Report
Thu 26 May 2016
SRR2099160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences676470
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACGTAAGGTCTAGCTAACCCTCACTCTCCTCCAACTTCTT16230.23992194775821546No Hit
TGCACGTAAAACTCCAAGTCGTTCACCGGCCTGATGGCCGCGC14200.20991322601150086No Hit
GGCACGGATCGGGCAGCCGCCCCGAGCGCGTGAGGCGCGCGAC13620.2013393055124396No Hit
TGCACGTAGGGATGGAATGGCAAGACCAGCAAGAAGATCACCA13170.19468712581489203No Hit
TGCACGTAAAGTAACCTACAGAAGGAGCGCTGTGCCCCCTGGA13050.19291321122887933No Hit
TGCACTCAGCGCTGCGGGCACCCAGCTACTCGGGAGGCTGAGG11650.17221754105873135No Hit
CGCACGTGCAGGAGCGGCAATCAAATTCTCGTGGTCTTGAGCC11530.17044362647271868No Hit
TGAACGGAAGTGGCCGAATGGGATACAAGCGCTGAATCCTCCT11400.16852188567120494No Hit
TGCACGTAAAGCTGATCATCGTTGCACGTATTTTTTTTTTTTT10260.15166969710408443No Hit
TGCACGTACCAACAAAAGCAACAGTCAACACTCTGATCATAAA9600.14191316688101469No Hit
TGCACGTATCCCGGTCAGGATTTGAATCCTGAACTTCCAGGCT9580.14161751445001255No Hit
TGCACGTAGCCGCGTTTATTCCAGGTACAGTATGTGCACCTGT8480.1253566307448963No Hit
TGCACGTACATTTGGCTAACGCTTAGTAGCGTTCGAGCTGCCG7630.11279140242730645No Hit
TGCCCTTACGATTGCGGCGACCGAGTTGCTCTTGCCCGGCGTC7500.11086966162579272No Hit
TGCACGTAGGAGTGGACGCGAGCCACAAGCTGCACTGTGAAAC7210.10658270137626207No Hit
TGAACGGAAGTGGATACAAGCGCTGAATCCTCCTCTGCTTCCA7040.1040696557127441No Hit
TGCTTGTAGTAATGAGCTACGTCCTATCATGGCACGTATCACC6830.10096530518722191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG200.001835315437.0249449
GTACCGT2400.037.0222056
TAGTTCG501.7826096E-1037.0222057
TACTCGT600.037.0222057
TAGTGCG308.2728E-637.0222057
TATAACG403.8091457E-837.0222057
TACCGTC1100.037.0222057
TACCGTA650.037.0222057
CGGAGTA308.277E-637.0194665
GCGTAAT200.001837985537.0139854
CGCGTAA1400.037.0112463
CGCTAGC308.302248E-637.00303610
TCCGGCG355.6231147E-737.00303311
CTATCCG251.232493E-437.00303310
CCCGGTC1050.037.00303310
CGGCCTA200.001847355236.9756826
ACGTACT38400.036.86944
CACGTAC133550.036.858823
CGTATGG10550.036.8440175
CGTAACG8900.036.811495
Produced by FastQC (version 0.11.5)