FastQCFastQC Report
Thu 26 May 2016
SRR2099159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147798
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACGTAAGGTCTAGCTAACCCTCACTCTCCTCCAACTTCTT4940.3342399761837102No Hit
GGCACGGATCGGGCAGCCGCCCCGAGCGCGTGAGGCGCGCGAC4140.2801120448179272No Hit
TGCACGTAAAGTAACCTACAGAAGGAGCGCTGTGCCCCCTGGA3910.26455026455026454No Hit
TGCACGTAGGGATGGAATGGCAAGACCAGCAAGAAGATCACCA3900.2638736654081923No Hit
TGAACGGAAGTGGCCGAATGGGATACAAGCGCTGAATCCTCCT3820.25846087227161396No Hit
CGCACGTGCAGGAGCGGCAATCAAATTCTCGTGGTCTTGAGCC3430.23207350573079474No Hit
TGCACGTAAAACTCCAAGTCGTTCACCGGCCTGATGGCCGCGC3200.2165117254631321No Hit
TGCACTCAGCGCTGCGGGCACCCAGCTACTCGGGAGGCTGAGG3040.2056861391899755No Hit
TGCACGTATCCCGGTCAGGATTTGAATCCTGAACTTCCAGGCT2560.17320938037050568No Hit
TGCTTGTAGTAATGAGCTACGTCCTATCATGGCACGTATCACC2520.17050298380221654No Hit
TGCACGTAGCCGCGTTTATTCCAGGTACAGTATGTGCACCTGT2490.16847318637599967No Hit
TGCACGTACCAACAAAAGCAACAGTCAACACTCTGATCATAAA2470.1671199880918551No Hit
TGAACGGAAGTGGATACAAGCGCTGAATCCTCCTCTGCTTCCA2240.1515582078241925No Hit
TGCCCTTACGATTGCGGCGACCGAGTTGCTCTTGCCCGGCGTC2230.1508816086821202No Hit
TGCACGTAGGAGTGGACGCGAGCCACAAGCTGCACTGTGAAAC2070.1400560224089636No Hit
TGAACGGAAGTGGTACAAGCGCTGAATCCTCCTCTGCTTCCAG1950.13193683270409615No Hit
TGCACGTATCTTCGTGACCAAGGAGGAATTCCAGGGTGAATGG1930.13058363441995155No Hit
TGCACGTACATTTGGCTAACGCTTAGTAGCGTTCGAGCTGCCG1910.12923043613580698No Hit
TGCACATGACCGCCGGAAAATCCAGCACCACCGACACGCCAGC1870.12652403956751784No Hit
TGCACGTATGACCATCTCCGTTGCACGTATTTTTTTTTTTTTT1760.11908144900472266No Hit
TGCGCGGAGCTCCGCGGGTCGAAGGTGATCGGCCCGCCACCGC1710.11569845329436122No Hit
TGCACGTCCCCAAGAATACAGCCTTTTTGACCCTGAGTTAGGC1590.10757926358949377No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTC251.2264411E-437.01015533
CCGCTCG251.2264411E-437.01015534
TGAATGG251.2264411E-437.01015537
TATCAGA452.588422E-936.9976357
GTATTCT900.036.9976356
TTTACGA452.588422E-936.9976357
GTAATCC452.588422E-936.9976356
GTACTGG452.588422E-936.9976356
CGTAAAG1800.036.9976355
GTAACGC900.036.9976356
GTAACCG452.588422E-936.9976356
GTAGTAA900.036.9976356
GTACGCT452.588422E-936.9976356
TAGTACC452.588422E-936.9976357
CCACAAA200.001837327136.9976324
TATCACT200.001837327136.997637
GTACCCG308.266647E-636.997636
GTACCAC750.036.997636
TGCCCGC355.5899363E-736.997639
GTACATC355.5899363E-736.997636
Produced by FastQC (version 0.11.5)