FastQCFastQC Report
Thu 26 May 2016
SRR2099158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587614
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGATTGGCGGCCACCCGATGCTGGTCCAGCGGACCGAGGCG44400.755598062673796No Hit
CTAGATCGCAACTGATTATGATTTTGCGATGGACTGGGATAAA33570.5712934000891742No Hit
CTAGATCGCGAATACGACTCACTATAGTGAGTCGTATTACCGG32780.5578492003253837No Hit
CTAGATCGCCAGGGTCGGACTGTAGAACTCTGAACCCCTATAG30900.5258554084824392Illumina DpnII expression Sequencing Primer (95% over 24bp)
CTAGATCGGACGTAGTTGTGGTTGAGCCCTTCCATTCCATCGC29810.5073058164032852No Hit
CTAGATCGTCGAGCCGACGATCCAACCCTAGAGTGAGTCGTAT24920.42408792166286036No Hit
CTAGATCGGGCAACGATCTGGCTCTAGATCGTTTTTTTTTTTT23790.40485761060832454No Hit
CTAGATCGAGCGGCGACTCACTATAGTGAGTCGTATTACCGGC22050.3752463351792163No Hit
CTAGATCGAAATGCGACGATCTAGCATTTGATCGTCGGACTGT21830.3715023808146164No Hit
CTAGATCGTGATCCGACGATCTAGGATCAGATCGTCGGACTGT21750.3701409428638528No Hit
CTAGATCGACGAAGTCGGACTGTAGAACTCTGAACCCCTATAG20590.35040009257778065Illumina DpnII expression Adapter 1 (95% over 23bp)
CTAGATCGCTCCCTGCCACCCGGGTTCAAGCGATTCTCCTGCC19260.32776618664633583No Hit
CTAGATCGTAGTTCGACTCACTATAGTGAGTCGTATTACCGGC18430.31364126790716357No Hit
CTAGATCGCGTTGGGCCGACGATCTAGCAACGGATCGTCGGAC16840.28658268863573705No Hit
CTAGATCGCGTTGAGTGAGTCGTATTACCGGCCGACGATCTAG16780.28556161017266435No Hit
CTAGATCGCAGTAAGTGAGTCGTATTACCGGCCGACGATCTAG14780.25152566140357446No Hit
CTAGATCGGCTCACGATCTAGTGAGCGATCGTCGGACTGTAGA14490.2465904488320564No Hit
CTAGATCGGCTCAACGATCTAGTGAGCGATCGTCGGACTGTAG14260.24267631472361106No Hit
CTAGATCGCGTTGCGGCCGACGATCTAGCAACGGATCGTCGGA14150.24080433754131114No Hit
CTAGATCGCGTTGGAGTCGTATTACCGGCCGACGATCTAGCAA14150.24080433754131114No Hit
CTAGATCGCGTTGCCGGCCGACGATCTAGCAACGGATCGTCGG13910.23672002368902034No Hit
CTAGATCGGCGTTCAGTCCGACGATCCGAGTCTAGATCGTCGG13510.22991283393520237No Hit
CTAGATCGCAGTACGACTCACTATAGTGAGTCGTATTACCGGC13280.225998699826757No Hit
CTAGATCGGTTCCGAGTCGTATTACCGGCCGACGATCTAGGGA12950.22038276827985717No Hit
CTAGATCGCGTTGACCGGCCGACGATCTAGCAACGGATCGTCG12930.2200424087921663No Hit
CTAGATCGGCTCAAGTGAGCGATCGTCGGACTGTAGAACTCTG12320.20966144441759388Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTAGATCGGCTCACGGCCGACGATCTAGTGAGCGATCGTCGGA11470.19519616619073063No Hit
CTAGATCGGCTCAGGCCGACGATCTAGTGAGCGATCGTCGGAC11360.1933241890084307No Hit
CTAGATCGAGCGGAGTGAGTCGTATTACCGGCCGACGATCTAG10510.1788589107815675No Hit
CTAGATCGCAGTACCGGCCGACGATCTAGTACTGGATCGTCGG10490.17851855129387662No Hit
CTAGATCGGCTCAAGTGAGTCGTATTACCGGCCGACGATCTAG10360.17630621462388577No Hit
CTAGACAGCGTATTAGCGCTTACGGGAACGTCATTTACAGCAT10250.1744342374415858No Hit
CTAGATCGCGTTGACGATCTAGCAACGGATCGTCGGACTGTAG10240.17426405769774034No Hit
CTAGATCGCAGTAGAACTCTGAACCCCTATAGTGAGTCGTATT10010.17034992358929502No Hit
CTAGATCGCGTTGTACCGGCCGACGATCTAGCAACGGATCGTC9870.16796740717545872No Hit
CTAGATCGGCCGAAGCATGGCCGATGCATCGCGAATGGAACCA9740.16575507050546787No Hit
CTAGATCGCGTTGAGCAACGGATCGTCGGACTGTAGAACTCTG9590.16320237434778614Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTAGATCGGCTCAACCGGCCGACGATCTAGTGAGCGATCGTCG9510.16184093639702254No Hit
CTAGATCGCAGTAGGCCGACGATCTAGTACTGGATCGTCGGAC9470.16116021742164074No Hit
CTAGATCGCGTTGGAACTCTGAACCCCTATAGTGAGTCGTATT9460.1609900376777953No Hit
CTAGATCGCAGTACGGCCGACGATCTAGTACTGGATCGTCGGA9450.16081985793394984No Hit
CTAGATCGCAGTAGAGTCGTATTACCGGCCGACGATCTAGTAC9420.1603093187024135No Hit
CTAGATCGCGTTGGCCGACGATCTAGCAACGGATCGTCGGACT9420.1603093187024135No Hit
CTAGATCGACGAAGGCCGACGATCTAGTTCGTGATCGTCGGAC9420.1603093187024135No Hit
CTAGATCGCAGTAACGATCTAGTACTGGATCGTCGGACTGTAG9280.1579268022885772No Hit
CTAGATCGGCTCAGAGTCGTATTACCGGCCGACGATCTAGTGA9170.15605482510627725No Hit
CTAGATCGCGTTGAGTCGTATTACCGGCCGACGATCTAGCAAC8810.14992835432784107No Hit
CTAGATCGCGTTGTAGCAACGGATCGTCGGACTGTAGAACTCT8680.14771601765785022Illumina DpnII expression Sequencing Primer (100% over 22bp)
CTGGGTCGTCGATGGCAAAAAGTGAGTGGAGATTGGATACAGC8500.14465278226863215No Hit
CTAGATCGCCAGGGGTCGGACTGTAGAACTCTGAACCCCTATA8480.14431242278094122Illumina NlaIII expression Adapter 1 (95% over 24bp)
CTAGATCGACGAAGGTCGGACTGTAGAACTCTGAACCCCTATA8450.14380188354940487Illumina DpnII expression Adapter 1 (95% over 23bp)
CTAGATCGCGTTGACCCCTATAGTGAGTCGTATTACCGGCCGA8370.1424404455986413No Hit
CTAGATCGGCTCACAGTCCGACGATCTAGTGAGCGATCGTCGG8340.14192990636710492No Hit
CTAGATCGCGTTGACTCTGAACCCCTATAGTGAGTCGTATTAC8320.14158954687941402No Hit
CTAGATCGCGTTGGAACCCCTATAGTGAGTCGTATTACCGGCC8280.14090882790403225No Hit
CTAGATCGGCTCAAGTCGTATTACCGGCCGACGATCTAGTGAG8200.13954738995326862No Hit
CTAGATCGGCTCACCGGCCGACGATCTAGTGAGCGATCGTCGG8180.13920703046557775No Hit
CTAAATCCCGTTCAAAATTAGCTGAGCATGGTGGCAGGCGCCT8080.13750523302712325No Hit
CTAGATCGGCTCAGACGATCTAGTGAGCGATCGTCGGACTGTA7770.1322296609679143No Hit
CTAGATCGCAGTAGAACCCCTATAGTGAGTCGTATTACCGGCC7640.13001732429792345No Hit
CTAGATCGGCTCAGAACTCTGAACCCCTATAGTGAGTCGTATT7640.13001732429792345No Hit
CTAGATCGACGAAAGTGAGTCGTATTACCGGCCGACGATCTAG7610.1295067850663871No Hit
CTAGATCGACGAAGAGTCGTATTACCGGCCGACGATCTAGTTC7570.12882606609100533No Hit
CTAGATCGCGTTGTGAGTCGTATTACCGGCCGACGATCTAGCA7560.12865588634715985No Hit
CTAGATCGCGTTGATCTAGCAACGGATCGTCGGACTGTAGAAC7390.12576283070178723No Hit
CTAGATCGAGCGGCGGCCGACGATCTAGCCGCTGATCGTCGGA7320.12457157249486908No Hit
CTAGATCGCGTTGGACGATCTAGCAACGGATCGTCGGACTGTA7310.12440139275102363No Hit
CTAGATCGAGCGGCCGGCCGACGATCTAGCCGCTGATCGTCGG7180.12218905608103278No Hit
CTAGATCGACGAACGGCCGACGATCTAGTTCGTGATCGTCGGA7170.12201887633718735No Hit
CTAGATCGGCCGAAAGCATGGCCGATGCATCGCGAATGGAACC7140.12150833710565098No Hit
CTAGATCGCAGTATGAGTCGTATTACCGGCCGACGATCTAGTA7120.12116797761796008No Hit
CTAGATCGAGCGGGCCGCTGATCGTCGGACTGTAGAACTCTGA7080.1204872586425783Illumina DpnII expression Sequencing Primer (100% over 24bp)
CTAGATCGGCTCATACCGGCCGACGATCTAGTGAGCGATCGTC7060.12014689915488738No Hit
CTAGATCGCCAGGAGTCGGACTGTAGAACTCTGAACCCCTATA7030.11963635992335105Illumina NlaIII expression Adapter 1 (95% over 24bp)
CTAGATCGCGTTGGCAACGGATCGTCGGACTGTAGAACTCTGA6960.1184451017164329Illumina DpnII expression Sequencing Primer (100% over 24bp)
CTAGATCGAGCGGGAGTCGTATTACCGGCCGACGATCTAGCCG6930.11793456248489656No Hit
CTAGATCGCGTTGAGAACTCTGAACCCCTATAGTGAGTCGTAT6920.1177643827410511No Hit
CTAGATCGCGTTGATTACCGGCCGACGATCTAGCAACGGATCG6880.11708366376566931No Hit
CTAGATCGCAGTAACCGGCCGACGATCTAGTACTGGATCGTCG6740.11470114735183301No Hit
CTAGATCGCAGTAACTCTGAACCCCTATAGTGAGTCGTATTAC6700.1140204283764512No Hit
CTAGATCGCGACTTACGACTCACTATAGGGAGTCGTATTACCG6700.1140204283764512No Hit
CTAGATCGGCTCAGCCGACGATCTAGTGAGCGATCGTCGGACT6640.1129993499133785No Hit
CTAGATCGCGTTGTCTAGCAACGGATCGTCGGACTGTAGAACT6640.1129993499133785No Hit
CTAGATCGCAGTAGCCGACGATCTAGTACTGGATCGTCGGACT6590.11214845119415127No Hit
CTAGATCGACGAAACCGGCCGACGATCTAGTTCGTGATCGTCG6550.11146773221876946No Hit
CTAGATCGCAGTATACCGGCCGACGATCTAGTACTGGATCGTC6470.11010629426800586No Hit
CTAGATCGGGTTGTCGGACTGTAGAACTCTGAACCCCTATAGT6460.10993611452416042Illumina NlaIII expression Adapter 1 (95% over 24bp)
CTAGATCGGGGATGAGTTCTACAGTGAGTCGTATTTCCGGCCT6420.10925539554877861No Hit
CTAGATCGCGTTGGATCGTCGGACTGTAGAACTCTGAACCCCT6390.10874485631724227Illumina DpnII expression Sequencing Primer (96% over 27bp)
CTAGATCGCTAGTCGACGATCTAGACTAGGATCGTCGGACTGT6360.10823431708570591No Hit
CTAGATCGTCGAGGCCGACGATCCAACCCTAGAGTGAGTCGTA6330.10772377785416958No Hit
CTAGATCGACGAAAGTCGTATTACCGGCCGACGATCTAGTTCG6210.10568162092802418No Hit
CTAGATCGCAGTAATTACCGGCCGACGATCTAGTACTGGATCG6140.10449036272110603No Hit
CTAGATCGCGAATCGGCCGACGATCTAGATTCGGATCGTCGGA6130.10432018297726058No Hit
CTAGATCGAGCGGACCGGCCGACGATCTAGCCGCTGATCGTCG6110.10397982348956969No Hit
CTAGATCGACGAAGCCGACGATCTAGTTCGTGATCGTCGGACT6030.10261838553880609No Hit
CTAGATCGAATACCGAGAAATACCCCAAGCCTTATTCGAACTA6000.10210784630726973No Hit
CTAGATCGGCTCAACTCTGAACCCCTATAGTGAGTCGTATTAC5920.10074640835650614No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGT200.001793938737.1964437
ACGACTT551.0913936E-1137.1964437
CCAGGTA950.037.0189979
ATTCGTC251.2291713E-437.0189979
CTGACCG251.2291713E-437.0189979
ACGTGCA403.810601E-837.0189979
GTCAACG251.2291713E-437.0189979
GTCAAAG251.2291713E-437.0189979
CGTACGG551.2732926E-1137.0189979
TAGATAC308.275869E-637.0189979
CAGTATG1700.037.0189979
CAGTAGG2650.037.0189979
CCGCCTC200.001836567737.0189979
CCGCCGT308.275869E-637.0189979
GATTACG200.001836567737.0189979
ACGAATG403.810601E-837.0189979
ACGAATC1200.037.0189979
CGTTGAT2900.037.0189939
ACGAATA1050.037.0189939
TCGTTCC1050.037.012696
Produced by FastQC (version 0.11.5)