##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2099158_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 587614 Sequences flagged as poor quality 0 Sequence length 43 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.875081260827685 37.0 35.0 37.0 31.0 37.0 2 34.82922292525365 37.0 35.0 37.0 31.0 37.0 3 33.172002368902035 35.0 32.0 36.0 28.0 37.0 4 35.52001143607879 37.0 35.0 39.0 30.0 39.0 5 33.150566528367264 35.0 32.0 37.0 25.0 39.0 6 35.09460632319856 37.0 34.0 39.0 30.0 39.0 7 35.90175012848571 39.0 35.0 39.0 30.0 39.0 8 35.277753763525034 38.0 35.0 39.0 28.0 39.0 9 30.820365069586497 31.0 30.0 33.0 27.0 34.0 10 31.00429363493722 31.0 31.0 34.0 27.0 34.0 11 30.970033048906256 31.0 30.0 34.0 27.0 34.0 12 34.52411106610802 35.0 35.0 37.0 31.0 37.0 13 34.580232942033376 35.0 35.0 37.0 31.0 37.0 14 31.40075627878165 31.0 31.0 33.0 30.0 34.0 15 31.46677751040649 31.0 31.0 34.0 30.0 34.0 16 31.493167283284606 31.0 31.0 34.0 30.0 34.0 17 35.12910686266835 35.0 35.0 37.0 32.0 37.0 18 35.2020986566011 35.0 35.0 37.0 33.0 37.0 19 35.16930161636721 35.0 35.0 37.0 32.0 37.0 20 35.08931543496241 35.0 35.0 37.0 32.0 37.0 21 35.159558826032054 35.0 35.0 37.0 32.0 37.0 22 36.791621710851004 38.0 37.0 39.0 33.0 39.0 23 36.91765172375062 38.0 37.0 39.0 33.0 39.0 24 37.052670630720165 39.0 37.0 39.0 33.0 39.0 25 37.16188518313042 39.0 37.0 39.0 34.0 39.0 26 37.22211009267989 39.0 37.0 39.0 34.0 39.0 27 38.509244163685686 40.0 38.0 41.0 35.0 41.0 28 38.61713982308114 40.0 38.0 41.0 35.0 41.0 29 38.685795437140705 40.0 38.0 41.0 35.0 41.0 30 38.73301010527319 40.0 38.0 41.0 36.0 41.0 31 38.77349246273914 40.0 38.0 41.0 36.0 41.0 32 38.848424305751735 40.0 38.0 41.0 36.0 41.0 33 38.86577242883934 40.0 38.0 41.0 36.0 41.0 34 38.852343545252495 40.0 38.0 41.0 35.0 41.0 35 38.843085767187304 40.0 38.0 41.0 35.0 41.0 36 38.84733004999881 40.0 38.0 41.0 35.0 41.0 37 38.851308852409915 40.0 38.0 41.0 35.0 41.0 38 38.8224361570691 40.0 38.0 41.0 35.0 41.0 39 38.78068596051149 40.0 38.0 41.0 35.0 41.0 40 38.72877262965144 40.0 38.0 41.0 35.0 41.0 41 38.682172310394236 40.0 38.0 41.0 35.0 41.0 42 38.461255177718705 40.0 38.0 41.0 35.0 41.0 43 38.51945835191129 40.0 38.0 41.0 35.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4450672794280166 1101 2 0.5463763810211404 1101 3 0.8048133315465975 1101 4 0.9803095042963719 1101 5 1.0107440590446402 1101 6 0.9476361489915277 1101 7 0.8126495387387465 1101 8 1.127207751875062 1101 9 0.36942901010320384 1101 10 0.5018554027650524 1101 11 0.5313703707466679 1101 12 0.5813311229441425 1101 13 0.45689222201213653 1101 14 0.2827619391234357 1101 15 0.3325251996488099 1101 16 0.4162987990626128 1101 17 0.2695208874098469 1101 18 0.23610756984559345 1101 19 0.4813791226839754 1101 20 0.17633631620574874 1101 21 0.2861872668462766 1101 22 0.21847032203878314 1101 23 0.35338348155501365 1101 24 0.5215322797911881 1101 25 0.298039290672385 1101 26 0.2395514690486067 1101 27 0.29804602956637893 1101 28 0.38203430120514525 1101 29 0.5374486104468232 1101 30 0.5163704469143369 1101 31 0.3784745408528565 1101 32 0.15664398260079793 1101 33 0.21944464059706092 1101 34 0.33388002936970906 1101 35 0.24233555961385633 1101 36 0.42645545583650346 1101 37 0.29180783961356127 1101 38 0.4517369855326905 1101 39 0.37778468509579 1101 40 0.37681599707605784 1101 41 0.2698354620546155 1101 42 0.6594049766600918 1101 43 0.5137361287646627 1102 1 0.6989682872096239 1102 2 0.6621679360664317 1102 3 0.3600372121436166 1102 4 0.5912930481157375 1102 5 0.6621613316559447 1102 6 0.6236024712265049 1102 7 0.6111595527711486 1102 8 0.8100767402664388 1102 9 0.34473325809402056 1102 10 0.5193933540343458 1102 11 0.47342675548962276 1102 12 0.43837283744854716 1102 13 0.6405610573227634 1102 14 0.17734415624296673 1102 15 0.15626036995114134 1102 16 0.2268524411087931 1102 17 0.24105552192955315 1102 18 0.21642138538329192 1102 19 0.31310893516005933 1102 20 0.25508743241955756 1102 21 0.2483150892726087 1102 22 0.14543422049522547 1102 23 0.08887525346469971 1102 24 0.1639241665114355 1102 25 0.21316749580059025 1102 26 0.41693889091364156 1102 27 0.337038247961587 1102 28 0.378366741567433 1102 29 0.3493085415604895 1102 30 0.3626021091161462 1102 31 0.3584796435445199 1102 32 0.3478660772557234 1102 33 0.3174839313229185 1102 34 0.4290337479562609 1102 35 0.34704151700638164 1102 36 0.28998396712621144 1102 37 0.449664709112227 1102 38 0.2179316532198996 1102 39 0.2925684585362802 1102 40 0.3167828295802053 1102 41 0.1733142221005366 1102 42 0.42899803699942396 1102 43 0.46503093677588936 1103 1 0.2569590380919422 1103 2 0.4230210454737531 1103 3 0.352589238430582 1103 4 0.4552945085027389 1103 5 0.31612506537871354 1103 6 0.2468591342422073 1103 7 0.28980626081545324 1103 8 0.5078946786867888 1103 9 0.4033641452791237 1103 10 0.31561581965440055 1103 11 0.2762331325128642 1103 12 0.20750390718202283 1103 13 0.0927528548157639 1103 14 0.2759336657963303 1103 15 0.4270672337700532 1103 16 0.35733923677631196 1103 17 0.4597995891073552 1103 18 0.3731647223675907 1103 19 0.32627797495560174 1103 20 0.15797291373877442 1103 21 0.36291165736314923 1103 22 0.3531243750528432 1103 23 0.3723655784008031 1103 24 0.3053224870663769 1103 25 0.4432916286958104 1103 26 0.26546998498690755 1103 27 0.30990182347576223 1103 28 0.334950652970619 1103 29 0.5197466576612584 1103 30 0.36444828168521326 1103 31 0.38637546927167676 1103 32 0.29480900046370806 1103 33 0.13121278613611054 1103 34 0.18895464366028847 1103 35 0.18367654195466088 1103 36 0.31437243006889304 1103 37 0.1979524053517281 1103 38 0.11465795235583442 1103 39 0.24656991892327795 1103 40 0.19128690649392155 1103 41 0.2655486142144454 1103 42 0.5580573043719426 1103 43 0.27133494067025765 1104 1 0.44763305656809393 1104 2 0.33981171645300634 1104 3 0.08981309261319836 1104 4 0.4256936173081982 1104 5 0.34737181428371855 1104 6 0.2838301482097876 1104 7 0.49904761158770583 1104 8 0.60902492003639 1104 9 0.21770017378238649 1104 10 0.29200571025396016 1104 11 0.32274965362788777 1104 12 0.3344418520341037 1104 13 0.3236323249617996 1104 14 0.29502351115818115 1104 15 0.3164382425943302 1104 16 0.25238190867667853 1104 17 0.22606441556171575 1104 18 0.18251174468603182 1104 19 0.22788303690320788 1104 20 0.08616652645488898 1104 21 0.12740438133948828 1104 22 0.2540444422431207 1104 23 0.3156699884344576 1104 24 0.18605508077681776 1104 25 0.1844197192086341 1104 26 0.06091137994482665 1104 27 0.3726104049638366 1104 28 0.37732915114884236 1104 29 0.41322529716347844 1104 30 0.30738213994740704 1104 31 0.2929964228638653 1104 32 0.2870893667138077 1104 33 0.306940826852383 1104 34 0.28486559901757147 1104 35 0.4152106145991681 1104 36 0.4255940702923482 1104 37 0.43808164258785354 1104 38 0.3099036679240328 1104 39 0.21056561257398698 1104 40 0.38973432963949506 1104 41 0.2547336457931735 1104 42 0.5293798702734662 1104 43 0.46640648521653816 1105 1 0.3902107218333484 1105 2 0.2596933443112661 1105 3 0.15880391306858854 1105 4 0.4677918490749704 1105 5 0.23873093311762972 1105 6 0.41192038832139843 1105 7 0.4081493894732162 1105 8 0.6676044323984485 1105 9 0.023181356757945082 1105 10 0.15599949769084986 1105 11 0.1302567200893705 1105 12 0.10971576310802078 1105 13 0.2575371907017754 1105 14 0.07163558024662109 1105 15 0.18487976015326524 1105 16 0.056863860544670075 1105 17 0.13140838249824327 1105 18 0.16185475297493213 1105 19 -0.0704230963575867 1105 20 0.10072064013909454 1105 21 0.0744473263400991 1105 22 -0.01131009833542862 1105 23 0.11459639084051076 1105 24 0.18416740049567437 1105 25 0.21162929778757444 1105 26 0.26836974622938925 1105 27 0.030245967500242443 1105 28 0.03821029529587605 1105 29 0.11581419418124739 1105 30 0.13302234435652593 1105 31 0.2057583919743493 1105 32 0.1765322871449584 1105 33 -0.016122326527941766 1105 34 0.01595506804304847 1105 35 0.1670974722421903 1105 36 0.1432898938134315 1105 37 0.15530362654970276 1105 38 0.12787798187125787 1105 39 0.14395934582644543 1105 40 0.006151334776049566 1105 41 0.10795964861647178 1105 42 0.3302290521872706 1105 43 0.1900965985322003 1106 1 0.33404570068867656 1106 2 0.18975901074767165 1106 3 0.15347983509443708 1106 4 0.30782310276055114 1106 5 -0.04660005267921008 1106 6 -0.06724452330901443 1106 7 0.17341820486586812 1106 8 0.30079167651416583 1106 9 0.1731758810448376 1106 10 0.14622850886130578 1106 11 0.2671790323566512 1106 12 0.2972526188693081 1106 13 0.3635027695267681 1106 14 0.12744889212639166 1106 15 0.1253450694046947 1106 16 0.056078306318262605 1106 17 0.1827495110005941 1106 18 0.010014281558156313 1106 19 0.07152423570650512 1106 20 0.0857477238585318 1106 21 0.04912110020156746 1106 22 -0.015804395169624286 1106 23 0.06939437914775937 1106 24 0.06149247252236023 1106 25 -0.03542540037427244 1106 26 0.08039062133258312 1106 27 -0.03385883764861575 1106 28 0.04446419147635794 1106 29 0.13590690221622737 1106 30 0.1723516537427443 1106 31 0.1443903903569108 1106 32 0.11500633226483359 1106 33 0.12888192767993445 1106 34 0.1424945858433304 1106 35 0.05853514177869101 1106 36 0.07160965018631771 1106 37 0.11944612987725378 1106 38 0.23669303755504956 1106 39 0.1329099885909244 1106 40 0.18217445070953175 1106 41 0.15376504177267236 1106 42 0.3516938475640856 1106 43 0.25576408795986083 1107 1 0.309975402699493 1107 2 0.31429163612116184 1107 3 0.30486479835051483 1107 4 0.1960549528776454 1107 5 0.7631027657008289 1107 6 0.18205895726919863 1107 7 0.3388223880201977 1107 8 0.3728955689473352 1107 9 -0.04231107688135083 1107 10 0.16042236333921878 1107 11 0.2749921632619241 1107 12 0.2323794055930435 1107 13 0.37567873657837225 1107 14 0.06437754431083675 1107 15 0.15977432725820506 1107 16 0.11426897093911492 1107 17 0.1319258010756954 1107 18 0.06456588456227053 1107 19 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2216 13 -0.2683278315661255 2216 14 0.4982738614583866 2216 15 0.7142195536246092 2216 16 0.7196642324920113 2216 17 0.7255453178479243 2216 18 0.5998227459275114 2216 19 0.844469479377743 2216 20 0.8485794959116149 2216 21 0.6547096470957356 2216 22 0.4823276445315017 2216 23 0.7115963419000693 2216 24 0.5596384522257196 2216 25 0.295684502603315 2216 26 0.7179366233399449 2216 27 0.6612105835624931 2216 28 0.7734914581207235 2216 29 0.6798527592475665 2216 30 0.7921032429029964 2216 31 0.6114501650864739 2216 32 0.5308365987976771 2216 33 0.5939691920938941 2216 34 0.5916414577068281 2216 35 0.6134587505664726 2216 36 0.6634533548813124 2216 37 0.8468075662550802 2216 38 0.8861856214738637 2216 39 0.8281466898287988 2216 40 0.6022703579248798 2216 41 0.7888924533930535 2216 42 1.0141227535527122 2216 43 0.8396341113790626 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 3.0 25 45.0 26 340.0 27 1362.0 28 3642.0 29 6233.0 30 9674.0 31 14755.0 32 21571.0 33 30775.0 34 46450.0 35 70433.0 36 116424.0 37 178689.0 38 87218.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 1.1751571272726655 2.4211130039943427 1.0114350191718249 95.39229484956117 2 1.3185638749576674 4.617526280230561 93.19122069267148 0.8726891521402838 3 1.2889545411226533 95.56575131720467 1.4810894337721747 1.6642047079005053 4 97.47367310402102 1.14037834489425 0.8338835830445941 0.5520649680401353 5 1.4387093480369633 96.60863497898265 1.1366382464559828 0.8160174265243955 6 0.8001919643844801 0.6643873732362846 97.49425213024307 1.0411685321361652 7 1.0610779258351628 1.23057810452511 2.1104133694116847 95.59793060022804 8 95.69899609773199 1.6056650107980335 1.2278614788455549 1.4674774126244243 9 24.02461966301325 20.35671928676591 17.220472762964175 38.398188287256666 10 32.7107146808746 22.23298409266935 14.246976415616249 30.809324810839794 11 31.89634772305141 21.22229467114526 28.81490397301949 18.066453632783837 12 24.21696900564385 21.932358925029583 32.7728073685017 21.07786470082487 13 30.994747460989043 29.288736411077153 22.82257995862668 16.893936169307125 14 31.103610044393605 26.17384317890895 16.92948770324373 25.793059073453712 15 30.513767932180656 25.629168716099265 18.61151437893257 25.245548972787507 16 28.102163694720613 24.136411132629686 23.305071765775025 24.456353406874676 17 24.991746282423495 23.798445918579205 23.201795736657058 28.008012062340242 18 24.126593523288285 23.45353149096429 21.832396492249153 30.58747849349827 19 26.077787951922062 23.454503987772874 20.446058691574788 30.02164936873028 20 24.85866379853167 25.357127054522927 24.449716306504698 25.334492840440703 21 23.587082676723156 27.02998907446045 24.17777656761071 25.205151681205688 22 23.77836470880544 24.35135990633307 25.002978145517297 26.867297239344197 23 22.677642125613072 24.39611717896442 29.14123897660709 23.785001718815412 24 23.679830637118926 28.22499123574319 24.970303634698972 23.124874492438913 25 22.745203483919717 25.290581912616105 28.432780702978487 23.531433900485695 26 24.202052521401704 23.831713668158688 26.43038276290485 25.535851047534763 27 23.82448341938756 24.690017596585516 25.660382495992266 25.82511648803466 28 25.459570398254638 23.898171248472636 24.841477568607964 25.800780784664763 29 23.19566926587862 26.85623555599424 26.160710942897893 23.78738423522925 30 25.558444829428844 25.93096828870653 25.12363558390372 23.386951297960906 31 27.691401809357195 24.360802706547858 26.006276250314837 21.941519233780113 32 25.521222445537767 24.55459327546038 24.972388101611788 24.95179617739007 33 25.359845068361203 23.915359402601027 27.12137559690545 23.60341993213232 34 26.42057541174989 26.51213211393874 25.945603746677243 21.121688727634126 35 25.797893160429535 27.122581303926076 27.50736032402444 19.572165211619954 36 23.724247550262586 24.804718743937347 27.11763164254085 24.353402063259214 37 25.048246285801806 23.78550399074216 27.384149350759856 23.782100372696178 38 24.772724952094404 23.93203701749788 26.311830555432646 24.98340747497507 39 25.272202500280798 25.866810525276797 25.651192789824613 23.20979418461779 40 25.820966643975495 22.731279782164737 26.605173587474475 24.842579986385296 41 24.651424000816874 22.330136741518537 27.844895805855973 25.173543451808612 42 27.408391347439302 26.63348151394136 23.523914022153487 22.434213116465852 43 29.39010153061745 23.96095342797725 24.916525699192995 21.732419342212296 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 5.5 7 11.0 8 55.5 9 100.0 10 155.5 11 211.0 12 211.0 13 317.0 14 423.0 15 1009.0 16 1595.0 17 1707.0 18 1819.0 19 1819.0 20 1907.5 21 1996.0 22 2036.5 23 2077.0 24 2458.5 25 2840.0 26 2840.0 27 3617.0 28 4394.0 29 4917.5 30 5441.0 31 5570.5 32 5700.0 33 5700.0 34 6363.5 35 7027.0 36 8150.0 37 9273.0 38 13833.0 39 18393.0 40 18393.0 41 18219.5 42 18046.0 43 25390.5 44 32735.0 45 46617.0 46 60499.0 47 60499.0 48 68013.0 49 75527.0 50 80498.0 51 85469.0 52 89455.0 53 93441.0 54 93441.0 55 76048.0 56 58655.0 57 49031.5 58 39408.0 59 32592.5 60 25777.0 61 25777.0 62 20263.5 63 14750.0 64 11280.5 65 7811.0 66 6088.5 67 4366.0 68 4366.0 69 5830.5 70 7295.0 71 4176.5 72 1058.0 73 796.0 74 534.0 75 534.0 76 460.0 77 386.0 78 406.0 79 426.0 80 273.5 81 121.0 82 121.0 83 65.5 84 10.0 85 5.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0056159315468998355 2 8.508987192272479E-4 3 0.0010210784630726973 4 3.403594876908991E-4 5 6.807189753817982E-4 6 8.508987192272479E-4 7 6.807189753817982E-4 8 0.0011912582069181468 9 0.13035768378561438 10 0.025356781832971985 11 0.03863080185291705 12 0.04237475621751694 13 0.04748014853288043 14 0.02416552362605384 15 0.009019526423808827 16 0.0013614379507635964 17 0.0 18 1.7017974384544955E-4 19 0.011572222581490571 20 0.0013614379507635964 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.007487908729199781 27 0.0 28 0.0 29 0.0 30 0.0 31 3.403594876908991E-4 32 8.508987192272479E-4 33 0.0 34 0.0 35 6.807189753817982E-4 36 0.0 37 6.807189753817982E-4 38 0.0 39 0.0 40 0.0023825164138362937 41 0.0015316176946090462 42 0.5815041847199012 43 0.0013614379507635964 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 587614.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.344913497636206 #Duplication Level Percentage of deduplicated Percentage of total 1 60.83086053412463 9.942751534170391 2 11.787183091259305 3.853209760148669 3 6.48029569472643 3.177596177082234 4 4.144932063095424 2.709942240994939 5 2.7226820761101567 2.225100150779253 6 2.072986620854808 2.0329672199777407 7 1.565932635743662 1.7916523432048932 8 1.2473319798011349 1.6310026650147886 9 0.9703784684262586 1.427467691375631 >10 6.835337602165652 20.2701092894315 >50 0.5403717007652663 6.230450601925754 >100 0.6778072778385132 24.034825582780535 >500 0.08850018220625748 10.238864288461473 >1k 0.03540007288250299 10.434060454652204 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAAGATTGGCGGCCACCCGATGCTGGTCCAGCGGACCGAGGCG 4440 0.755598062673796 No Hit CTAGATCGCAACTGATTATGATTTTGCGATGGACTGGGATAAA 3357 0.5712934000891742 No Hit CTAGATCGCGAATACGACTCACTATAGTGAGTCGTATTACCGG 3278 0.5578492003253837 No Hit CTAGATCGCCAGGGTCGGACTGTAGAACTCTGAACCCCTATAG 3090 0.5258554084824392 Illumina DpnII expression Sequencing Primer (95% over 24bp) CTAGATCGGACGTAGTTGTGGTTGAGCCCTTCCATTCCATCGC 2981 0.5073058164032852 No Hit CTAGATCGTCGAGCCGACGATCCAACCCTAGAGTGAGTCGTAT 2492 0.42408792166286036 No Hit CTAGATCGGGCAACGATCTGGCTCTAGATCGTTTTTTTTTTTT 2379 0.40485761060832454 No Hit CTAGATCGAGCGGCGACTCACTATAGTGAGTCGTATTACCGGC 2205 0.3752463351792163 No Hit CTAGATCGAAATGCGACGATCTAGCATTTGATCGTCGGACTGT 2183 0.3715023808146164 No Hit CTAGATCGTGATCCGACGATCTAGGATCAGATCGTCGGACTGT 2175 0.3701409428638528 No Hit CTAGATCGACGAAGTCGGACTGTAGAACTCTGAACCCCTATAG 2059 0.35040009257778065 Illumina DpnII expression Adapter 1 (95% over 23bp) CTAGATCGCTCCCTGCCACCCGGGTTCAAGCGATTCTCCTGCC 1926 0.32776618664633583 No Hit CTAGATCGTAGTTCGACTCACTATAGTGAGTCGTATTACCGGC 1843 0.31364126790716357 No Hit CTAGATCGCGTTGGGCCGACGATCTAGCAACGGATCGTCGGAC 1684 0.28658268863573705 No Hit CTAGATCGCGTTGAGTGAGTCGTATTACCGGCCGACGATCTAG 1678 0.28556161017266435 No Hit CTAGATCGCAGTAAGTGAGTCGTATTACCGGCCGACGATCTAG 1478 0.25152566140357446 No Hit CTAGATCGGCTCACGATCTAGTGAGCGATCGTCGGACTGTAGA 1449 0.2465904488320564 No Hit CTAGATCGGCTCAACGATCTAGTGAGCGATCGTCGGACTGTAG 1426 0.24267631472361106 No Hit CTAGATCGCGTTGCGGCCGACGATCTAGCAACGGATCGTCGGA 1415 0.24080433754131114 No Hit CTAGATCGCGTTGGAGTCGTATTACCGGCCGACGATCTAGCAA 1415 0.24080433754131114 No Hit CTAGATCGCGTTGCCGGCCGACGATCTAGCAACGGATCGTCGG 1391 0.23672002368902034 No Hit CTAGATCGGCGTTCAGTCCGACGATCCGAGTCTAGATCGTCGG 1351 0.22991283393520237 No Hit CTAGATCGCAGTACGACTCACTATAGTGAGTCGTATTACCGGC 1328 0.225998699826757 No Hit CTAGATCGGTTCCGAGTCGTATTACCGGCCGACGATCTAGGGA 1295 0.22038276827985717 No Hit CTAGATCGCGTTGACCGGCCGACGATCTAGCAACGGATCGTCG 1293 0.2200424087921663 No Hit CTAGATCGGCTCAAGTGAGCGATCGTCGGACTGTAGAACTCTG 1232 0.20966144441759388 Illumina DpnII expression Sequencing Primer (100% over 23bp) CTAGATCGGCTCACGGCCGACGATCTAGTGAGCGATCGTCGGA 1147 0.19519616619073063 No Hit CTAGATCGGCTCAGGCCGACGATCTAGTGAGCGATCGTCGGAC 1136 0.1933241890084307 No Hit CTAGATCGAGCGGAGTGAGTCGTATTACCGGCCGACGATCTAG 1051 0.1788589107815675 No Hit CTAGATCGCAGTACCGGCCGACGATCTAGTACTGGATCGTCGG 1049 0.17851855129387662 No Hit CTAGATCGGCTCAAGTGAGTCGTATTACCGGCCGACGATCTAG 1036 0.17630621462388577 No Hit CTAGACAGCGTATTAGCGCTTACGGGAACGTCATTTACAGCAT 1025 0.1744342374415858 No Hit CTAGATCGCGTTGACGATCTAGCAACGGATCGTCGGACTGTAG 1024 0.17426405769774034 No Hit CTAGATCGCAGTAGAACTCTGAACCCCTATAGTGAGTCGTATT 1001 0.17034992358929502 No Hit CTAGATCGCGTTGTACCGGCCGACGATCTAGCAACGGATCGTC 987 0.16796740717545872 No Hit CTAGATCGGCCGAAGCATGGCCGATGCATCGCGAATGGAACCA 974 0.16575507050546787 No Hit CTAGATCGCGTTGAGCAACGGATCGTCGGACTGTAGAACTCTG 959 0.16320237434778614 Illumina DpnII expression Sequencing Primer (100% over 23bp) CTAGATCGGCTCAACCGGCCGACGATCTAGTGAGCGATCGTCG 951 0.16184093639702254 No Hit CTAGATCGCAGTAGGCCGACGATCTAGTACTGGATCGTCGGAC 947 0.16116021742164074 No Hit CTAGATCGCGTTGGAACTCTGAACCCCTATAGTGAGTCGTATT 946 0.1609900376777953 No Hit CTAGATCGCAGTACGGCCGACGATCTAGTACTGGATCGTCGGA 945 0.16081985793394984 No Hit CTAGATCGCAGTAGAGTCGTATTACCGGCCGACGATCTAGTAC 942 0.1603093187024135 No Hit CTAGATCGCGTTGGCCGACGATCTAGCAACGGATCGTCGGACT 942 0.1603093187024135 No Hit CTAGATCGACGAAGGCCGACGATCTAGTTCGTGATCGTCGGAC 942 0.1603093187024135 No Hit CTAGATCGCAGTAACGATCTAGTACTGGATCGTCGGACTGTAG 928 0.1579268022885772 No Hit CTAGATCGGCTCAGAGTCGTATTACCGGCCGACGATCTAGTGA 917 0.15605482510627725 No Hit CTAGATCGCGTTGAGTCGTATTACCGGCCGACGATCTAGCAAC 881 0.14992835432784107 No Hit CTAGATCGCGTTGTAGCAACGGATCGTCGGACTGTAGAACTCT 868 0.14771601765785022 Illumina DpnII expression Sequencing Primer (100% over 22bp) CTGGGTCGTCGATGGCAAAAAGTGAGTGGAGATTGGATACAGC 850 0.14465278226863215 No Hit CTAGATCGCCAGGGGTCGGACTGTAGAACTCTGAACCCCTATA 848 0.14431242278094122 Illumina NlaIII expression Adapter 1 (95% over 24bp) CTAGATCGACGAAGGTCGGACTGTAGAACTCTGAACCCCTATA 845 0.14380188354940487 Illumina DpnII expression Adapter 1 (95% over 23bp) CTAGATCGCGTTGACCCCTATAGTGAGTCGTATTACCGGCCGA 837 0.1424404455986413 No Hit CTAGATCGGCTCACAGTCCGACGATCTAGTGAGCGATCGTCGG 834 0.14192990636710492 No Hit CTAGATCGCGTTGACTCTGAACCCCTATAGTGAGTCGTATTAC 832 0.14158954687941402 No Hit CTAGATCGCGTTGGAACCCCTATAGTGAGTCGTATTACCGGCC 828 0.14090882790403225 No Hit CTAGATCGGCTCAAGTCGTATTACCGGCCGACGATCTAGTGAG 820 0.13954738995326862 No Hit CTAGATCGGCTCACCGGCCGACGATCTAGTGAGCGATCGTCGG 818 0.13920703046557775 No Hit CTAAATCCCGTTCAAAATTAGCTGAGCATGGTGGCAGGCGCCT 808 0.13750523302712325 No Hit CTAGATCGGCTCAGACGATCTAGTGAGCGATCGTCGGACTGTA 777 0.1322296609679143 No Hit CTAGATCGCAGTAGAACCCCTATAGTGAGTCGTATTACCGGCC 764 0.13001732429792345 No Hit CTAGATCGGCTCAGAACTCTGAACCCCTATAGTGAGTCGTATT 764 0.13001732429792345 No Hit CTAGATCGACGAAAGTGAGTCGTATTACCGGCCGACGATCTAG 761 0.1295067850663871 No Hit CTAGATCGACGAAGAGTCGTATTACCGGCCGACGATCTAGTTC 757 0.12882606609100533 No Hit CTAGATCGCGTTGTGAGTCGTATTACCGGCCGACGATCTAGCA 756 0.12865588634715985 No Hit CTAGATCGCGTTGATCTAGCAACGGATCGTCGGACTGTAGAAC 739 0.12576283070178723 No Hit CTAGATCGAGCGGCGGCCGACGATCTAGCCGCTGATCGTCGGA 732 0.12457157249486908 No Hit CTAGATCGCGTTGGACGATCTAGCAACGGATCGTCGGACTGTA 731 0.12440139275102363 No Hit CTAGATCGAGCGGCCGGCCGACGATCTAGCCGCTGATCGTCGG 718 0.12218905608103278 No Hit CTAGATCGACGAACGGCCGACGATCTAGTTCGTGATCGTCGGA 717 0.12201887633718735 No Hit CTAGATCGGCCGAAAGCATGGCCGATGCATCGCGAATGGAACC 714 0.12150833710565098 No Hit CTAGATCGCAGTATGAGTCGTATTACCGGCCGACGATCTAGTA 712 0.12116797761796008 No Hit CTAGATCGAGCGGGCCGCTGATCGTCGGACTGTAGAACTCTGA 708 0.1204872586425783 Illumina DpnII expression Sequencing Primer (100% over 24bp) CTAGATCGGCTCATACCGGCCGACGATCTAGTGAGCGATCGTC 706 0.12014689915488738 No Hit CTAGATCGCCAGGAGTCGGACTGTAGAACTCTGAACCCCTATA 703 0.11963635992335105 Illumina NlaIII expression Adapter 1 (95% over 24bp) CTAGATCGCGTTGGCAACGGATCGTCGGACTGTAGAACTCTGA 696 0.1184451017164329 Illumina DpnII expression Sequencing Primer (100% over 24bp) CTAGATCGAGCGGGAGTCGTATTACCGGCCGACGATCTAGCCG 693 0.11793456248489656 No Hit CTAGATCGCGTTGAGAACTCTGAACCCCTATAGTGAGTCGTAT 692 0.1177643827410511 No Hit CTAGATCGCGTTGATTACCGGCCGACGATCTAGCAACGGATCG 688 0.11708366376566931 No Hit CTAGATCGCAGTAACCGGCCGACGATCTAGTACTGGATCGTCG 674 0.11470114735183301 No Hit CTAGATCGCAGTAACTCTGAACCCCTATAGTGAGTCGTATTAC 670 0.1140204283764512 No Hit CTAGATCGCGACTTACGACTCACTATAGGGAGTCGTATTACCG 670 0.1140204283764512 No Hit CTAGATCGGCTCAGCCGACGATCTAGTGAGCGATCGTCGGACT 664 0.1129993499133785 No Hit CTAGATCGCGTTGTCTAGCAACGGATCGTCGGACTGTAGAACT 664 0.1129993499133785 No Hit CTAGATCGCAGTAGCCGACGATCTAGTACTGGATCGTCGGACT 659 0.11214845119415127 No Hit CTAGATCGACGAAACCGGCCGACGATCTAGTTCGTGATCGTCG 655 0.11146773221876946 No Hit CTAGATCGCAGTATACCGGCCGACGATCTAGTACTGGATCGTC 647 0.11010629426800586 No Hit CTAGATCGGGTTGTCGGACTGTAGAACTCTGAACCCCTATAGT 646 0.10993611452416042 Illumina NlaIII expression Adapter 1 (95% over 24bp) CTAGATCGGGGATGAGTTCTACAGTGAGTCGTATTTCCGGCCT 642 0.10925539554877861 No Hit CTAGATCGCGTTGGATCGTCGGACTGTAGAACTCTGAACCCCT 639 0.10874485631724227 Illumina DpnII expression Sequencing Primer (96% over 27bp) CTAGATCGCTAGTCGACGATCTAGACTAGGATCGTCGGACTGT 636 0.10823431708570591 No Hit CTAGATCGTCGAGGCCGACGATCCAACCCTAGAGTGAGTCGTA 633 0.10772377785416958 No Hit CTAGATCGACGAAAGTCGTATTACCGGCCGACGATCTAGTTCG 621 0.10568162092802418 No Hit CTAGATCGCAGTAATTACCGGCCGACGATCTAGTACTGGATCG 614 0.10449036272110603 No Hit CTAGATCGCGAATCGGCCGACGATCTAGATTCGGATCGTCGGA 613 0.10432018297726058 No Hit CTAGATCGAGCGGACCGGCCGACGATCTAGCCGCTGATCGTCG 611 0.10397982348956969 No Hit CTAGATCGACGAAGCCGACGATCTAGTTCGTGATCGTCGGACT 603 0.10261838553880609 No Hit CTAGATCGAATACCGAGAAATACCCCAAGCCTTATTCGAACTA 600 0.10210784630726973 No Hit CTAGATCGGCTCAACTCTGAACCCCTATAGTGAGTCGTATTAC 592 0.10074640835650614 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0015316176946090462 0.0 0.0 0.0 0.0 4 0.0015316176946090462 0.0 0.002722875901527193 0.0 0.0 5 0.0015316176946090462 0.0 0.002722875901527193 0.0 0.0 6 0.0015316176946090462 0.0 0.002722875901527193 0.0 0.0 7 0.0015316176946090462 0.0 0.002722875901527193 0.0 0.0 8 0.0015316176946090462 0.0 0.002722875901527193 0.0 0.0 9 0.0015316176946090462 0.0 0.002722875901527193 0.0 0.0 10 0.0015316176946090462 0.0 0.02671821978373558 0.0 0.0 11 0.0015316176946090462 0.0 0.02705857927142648 0.0 0.0 12 0.0015316176946090462 0.0 0.028930556453726424 0.0 0.0 13 0.0015316176946090462 0.0 0.1809010677077129 0.0 0.0 14 0.0015316176946090462 0.0 0.7840180798959862 0.0 0.0 15 0.0015316176946090462 0.0 1.3423778194529061 0.0 0.0 16 0.0015316176946090462 0.0 1.7601690905934848 0.0 0.0 17 0.0015316176946090462 0.0 2.2009346271531993 0.0 0.0 18 0.0015316176946090462 0.0 2.7079000840687937 0.0 0.0 19 0.0015316176946090462 0.0 3.1583658660276983 0.0 0.0 20 0.0015316176946090462 0.0 4.040407478378664 0.0 0.0 21 0.0015316176946090462 0.0 5.30535351438189 0.0 0.0 22 0.0015316176946090462 0.0 6.015853944936642 0.0 0.0 23 0.0015316176946090462 0.0 6.437218990697975 0.0 0.0 24 0.0015316176946090462 0.0 7.132233064562791 0.0 0.0 25 0.0015316176946090462 0.0 7.944671161680968 0.0 0.0 26 0.0015316176946090462 0.0 8.60054389446133 0.0 0.0 27 0.0015316176946090462 0.0 9.536702665355149 1.7017974384544958E-4 0.0 28 0.0015316176946090462 0.0 11.084487435629512 1.7017974384544958E-4 0.0 29 0.0015316176946090462 0.0 12.171935998801935 1.7017974384544958E-4 0.0 30 0.0015316176946090462 0.0 14.249490311667182 1.7017974384544958E-4 0.0 31 0.0015316176946090462 0.0 16.088452623661112 1.7017974384544958E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGATGT 20 0.0017939387 37.19644 37 ACGACTT 55 1.0913936E-11 37.19644 37 CCAGGTA 95 0.0 37.018997 9 ATTCGTC 25 1.2291713E-4 37.018997 9 CTGACCG 25 1.2291713E-4 37.018997 9 ACGTGCA 40 3.810601E-8 37.018997 9 GTCAACG 25 1.2291713E-4 37.018997 9 GTCAAAG 25 1.2291713E-4 37.018997 9 CGTACGG 55 1.2732926E-11 37.018997 9 TAGATAC 30 8.275869E-6 37.018997 9 CAGTATG 170 0.0 37.018997 9 CAGTAGG 265 0.0 37.018997 9 CCGCCTC 20 0.0018365677 37.018997 9 CCGCCGT 30 8.275869E-6 37.018997 9 GATTACG 20 0.0018365677 37.018997 9 ACGAATG 40 3.810601E-8 37.018997 9 ACGAATC 120 0.0 37.018997 9 CGTTGAT 290 0.0 37.018993 9 ACGAATA 105 0.0 37.018993 9 TCGTTCC 105 0.0 37.01269 6 >>END_MODULE