FastQCFastQC Report
Thu 26 May 2016
SRR2099157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148955
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGATTGGCGGCCACCCGATGCTGGTCCAGCGGACCGAGGCG13160.883488301836125No Hit
CTAGATCGTAGTTCGACTCACTATAGTGAGTCGTATTACCGGC12060.8096404954516464No Hit
CTAGATCGCAACTGATTATGATTTTGCGATGGACTGGGATAAA9190.6169648551575979No Hit
CTAGATCGCGAATACGACTCACTATAGTGAGTCGTATTACCGG9040.6068946997415327No Hit
CTAGATCGCCAGGGTCGGACTGTAGAACTCTGAACCCCTATAG7910.531032862273841Illumina DpnII expression Sequencing Primer (95% over 24bp)
CTAGATCGGACGTAGTTGTGGTTGAGCCCTTCCATTCCATCGC7760.5209627068577759No Hit
CTAGATCGTGATCCGACGATCTAGGATCAGATCGTCGGACTGT6750.45315699372293644No Hit
CTAGATCGTCGAGCCGACGATCCAACCCTAGAGTGAGTCGTAT6620.4444295256956799No Hit
CTAGATCGAGCGGCGACTCACTATAGTGAGTCGTATTACCGGC5890.39542143600416235No Hit
CTAGATCGCTCCCTGCCACCCGGGTTCAAGCGATTCTCCTGCC5600.3759524688664362No Hit
CTAGATCGAAATGCGACGATCTAGCATTTGATCGTCGGACTGT5450.3658823134503709No Hit
CTAGATCGGGCAACGATCTGGCTCTAGATCGTTTTTTTTTTTT5100.34238528414621866No Hit
CTAGATCGACGAAGTCGGACTGTAGAACTCTGAACCCCTATAG4980.33432915981336647Illumina DpnII expression Adapter 1 (95% over 23bp)
CTAGATCGCGTTGGGCCGACGATCTAGCAACGGATCGTCGGAC4470.3000906313987446No Hit
CTAGATCGCGTTGAGTGAGTCGTATTACCGGCCGACGATCTAG4460.29941928770434023No Hit
CTAGATCGCAGTAAGTGAGTCGTATTACCGGCCGACGATCTAG4100.2752509147057836No Hit
CTAGATCGCGTTGGAGTCGTATTACCGGCCGACGATCTAGCAA3870.25981000973448354No Hit
CTAGATCGGCTCAACGATCTAGTGAGCGATCGTCGGACTGTAG3700.24839716692960961No Hit
CTAGATCGGCTCACGATCTAGTGAGCGATCGTCGGACTGTAGA3660.2457117921519922No Hit
CTAGACAGCGTATTAGCGCTTACGGGAACGTCATTTACAGCAT3590.24101238629116178No Hit
CTAGATCGGTTCCGAGTCGTATTACCGGCCGACGATCTAGGGA3570.23966969890235304No Hit
CTAGATCGCGTTGCGGCCGACGATCTAGCAACGGATCGTCGGA3550.23832701151354435No Hit
CTAGATCGGCGTTCAGTCCGACGATCCGAGTCTAGATCGTCGG3550.23832701151354435No Hit
CTAGATCGCGTTGCCGGCCGACGATCTAGCAACGGATCGTCGG3410.22892819979188345No Hit
CTAGATCGCGTTGACCGGCCGACGATCTAGCAACGGATCGTCG3270.21952938807022257No Hit
CTAGATCGCAGTACGACTCACTATAGTGAGTCGTATTACCGGC3170.21281595112617904No Hit
CTAGATCGGCTCAGGCCGACGATCTAGTGAGCGATCGTCGGAC3100.20811654526534862No Hit
CTAGATCGAGCGGAGTGAGTCGTATTACCGGCCGACGATCTAG3040.20408848309892252No Hit
CTAGATCGGCTCACGGCCGACGATCTAGTGAGCGATCGTCGGA2890.19401832768285726No Hit
CTAGATCGCAGTAGAGTCGTATTACCGGCCGACGATCTAGTAC2880.19334698398845288No Hit
CTAGATCGGCTCAAGTGAGTCGTATTACCGGCCGACGATCTAG2810.18864757812762242No Hit
CTAGATCGCAGTAGAACTCTGAACCCCTATAGTGAGTCGTATT2800.1879762344332181No Hit
CTAGATCGGCCGAAGCATGGCCGATGCATCGCGAATGGAACCA2730.18327682857238764No Hit
CTAAATCCCGTTCAAAATTAGCTGAGCATGGTGGCAGGCGCCT2610.17522070423953542No Hit
CTAGATCGCAGTACCGGCCGACGATCTAGTACTGGATCGTCGG2560.17186398576751366No Hit
CTAGATCGACGAAGGCCGACGATCTAGTTCGTGATCGTCGGAC2560.17186398576751366No Hit
CTAGATCGCGTTGACGATCTAGCAACGGATCGTCGGACTGTAG2510.16850726729549192No Hit
CTAGATCGCGTTGTACCGGCCGACGATCTAGCAACGGATCGTC2500.16783592360108757No Hit
CTAGATCGCAGTAGGCCGACGATCTAGTACTGGATCGTCGGAC2450.16447920512906583No Hit
CTAGATCGCGTTGGCCGACGATCTAGCAACGGATCGTCGGACT2430.16313651774025711No Hit
CTAGATCGGCTCAGAGTCGTATTACCGGCCGACGATCTAGTGA2420.16246517404585278No Hit
CTAGATCGGCTCAACCGGCCGACGATCTAGTGAGCGATCGTCG2400.16112248665704407No Hit
CTAGATCGCAGTAACGATCTAGTACTGGATCGTCGGACTGTAG2340.15709442449061797No Hit
CTAGATCGCGTTGACCCCTATAGTGAGTCGTATTACCGGCCGA2310.15508039340740493No Hit
CTAGATCGGCTCACAGTCCGACGATCTAGTGAGCGATCGTCGG2280.15306636232419185No Hit
CTAGATCGCGTTGGAACTCTGAACCCCTATAGTGAGTCGTATT2280.15306636232419185No Hit
CTAGATCGGCTCACCGGCCGACGATCTAGTGAGCGATCGTCGG2280.15306636232419185No Hit
CTAGATCGCAGTACGGCCGACGATCTAGTACTGGATCGTCGGA2240.15038098754657447No Hit
CTAGATCGCGTTGGAACCCCTATAGTGAGTCGTATTACCGGCC2230.14970964385217012No Hit
CTAGATCGCAGTAGAACCCCTATAGTGAGTCGTATTACCGGCC2160.14501023799133966No Hit
CTAGATCGCGTTGACTCTGAACCCCTATAGTGAGTCGTATTAC2160.14501023799133966No Hit
CTGGGTCGTCGATGGCAAAAAGTGAGTGGAGATTGGATACAGC2150.1443388942969353No Hit
CTAGATCGCCAGGGGTCGGACTGTAGAACTCTGAACCCCTATA2140.14366755060253097Illumina NlaIII expression Adapter 1 (95% over 24bp)
CTAGATCGGCCGAAAGCATGGCCGATGCATCGCGAATGGAACC2130.14299620690812662No Hit
CTAGATCGCGTTGAGTCGTATTACCGGCCGACGATCTAGCAAC2100.14098217582491357No Hit
CTAGATCGGCTCAAGTCGTATTACCGGCCGACGATCTAGTGAG2090.1403108321305092No Hit
CTAGATCGGCTCAGACGATCTAGTGAGCGATCGTCGGACTGTA2090.1403108321305092No Hit
CTAGATCGGCTCAGAACTCTGAACCCCTATAGTGAGTCGTATT2070.13896814474170052No Hit
CTAGATCGGCTCATACCGGCCGACGATCTAGTGAGCGATCGTC2040.13695411365848748No Hit
CTAGATCGCGTTGTGAGTCGTATTACCGGCCGACGATCTAGCA2040.13695411365848748No Hit
CTAGATCGACGAAAGTGAGTCGTATTACCGGCCGACGATCTAG1990.1335973951864657No Hit
CTAGATCCATTAAGATCGTTTTTTTTTTTTTTTTTTTTTTTTT1960.13158336410325266No Hit
CTAGATCGCGACTTACGACTCACTATAGGGAGTCGTATTACCG1960.13158336410325266No Hit
CTAGATCGACGAAGGTCGGACTGTAGAACTCTGAACCCCTATA1930.1295693330200396Illumina DpnII expression Adapter 1 (95% over 23bp)
CTAGATCGCAGTAACCGGCCGACGATCTAGTACTGGATCGTCG1890.1268839582424222No Hit
CTAGATCGACGAAGAGTCGTATTACCGGCCGACGATCTAGTTC1860.12486992715920915No Hit
CTAGATCGACGAACGGCCGACGATCTAGTTCGTGATCGTCGGA1860.12486992715920915No Hit
CTAGATCGTAGTTCCGGCCGACGATCTAGAACTAGATCGTCGG1810.12151320868718742No Hit
CTAGATCGCGTTGAGAACTCTGAACCCCTATAGTGAGTCGTAT1790.1201705212983787No Hit
CTAGATCGAGCGGGAGTCGTATTACCGGCCGACGATCTAGCCG1780.11949917760397435No Hit
CTAGATCGGCTCAAGAACTCTGAACCCCTATAGTGAGTCGTAT1760.11815649021516565No Hit
CTAGATCGAGCGGCCGGCCGACGATCTAGCCGCTGATCGTCGG1760.11815649021516565No Hit
CTAGATCGGCTCAGCCGACGATCTAGTGAGCGATCGTCGGACT1760.11815649021516565No Hit
CTAGATCGAGCGGCGGCCGACGATCTAGCCGCTGATCGTCGGA1740.11681380282635696No Hit
CTAGATCGCAGTATGAGTCGTATTACCGGCCGACGATCTAGTA1740.11681380282635696No Hit
CTAGATCGGGGATGAGTTCTACAGTGAGTCGTATTTCCGGCCT1740.11681380282635696No Hit
CTAGATCGCGTTGGACGATCTAGCAACGGATCGTCGGACTGTA1730.1161424591319526No Hit
CTAGATCGTGATCGGCCGACGATCTAGGATCAGATCGTCGGAC1720.11547111543754826No Hit
CTAGATCGACGAAACCGGCCGACGATCTAGTTCGTGATCGTCG1710.11479977174314392No Hit
CTAGATCGCAGTAGCCGACGATCTAGTACTGGATCGTCGGACT1710.11479977174314392No Hit
CTAGATCGACGAAAGTCGTATTACCGGCCGACGATCTAGTTCG1690.1134570843543352No Hit
CTAGATCGCGTTGATTACCGGCCGACGATCTAGCAACGGATCG1690.1134570843543352No Hit
CTAGATCGTCGAGTCCGACGATCCAACCCTAGAGTGAGTCGTA1670.1121143969655265No Hit
CTAGATCGCAGTAACTCTGAACCCCTATAGTGAGTCGTATTAC1670.1121143969655265No Hit
CTAGATCGCCAGGAGTCGGACTGTAGAACTCTGAACCCCTATA1630.10942902218790909Illumina NlaIII expression Adapter 1 (95% over 24bp)
CTAGATCGGCTCAACCCCTATAGTGAGTCGTATTACCGGCCGA1630.10942902218790909No Hit
CTAGATCGTCGAGGCCGACGATCCAACCCTAGAGTGAGTCGTA1620.10875767849350476No Hit
CTAGATCGCGAATCGGCCGACGATCTAGATTCGGATCGTCGGA1610.10808633479910039No Hit
CATGATCGGTGGGCGCTGATCAGGCTGCGCGAAGGCGACGCTG1600.10741499110469604No Hit
CTAGATCGCTAGTCGACGATCTAGACTAGGATCGTCGGACTGT1590.1067436474102917No Hit
CTAGATCGGGTTGTCGGACTGTAGAACTCTGAACCCCTATAGT1590.1067436474102917Illumina NlaIII expression Adapter 1 (95% over 24bp)
CTAGATCGGCTCAACTCTGAACCCCTATAGTGAGTCGTATTAC1580.10607230371588734No Hit
CTAGATCGACGAAGCCGACGATCTAGTTCGTGATCGTCGGACT1550.10405827263267431No Hit
CTAGATCGGCTCATGAGTCGTATTACCGGCCGACGATCTAGTG1530.10271558524386559No Hit
CTAGATCGAGCGGACCGGCCGACGATCTAGCCGCTGATCGTCG1530.10271558524386559No Hit
CTAGATCGGCTCAAGTGAGCGATCGTCGGACTGTAGAACTCTG1520.10204424154946126Illumina DpnII expression Sequencing Primer (100% over 23bp)
CTAGATCGTAGTTAGTGAGTCGTATTACCGGCCGACGATCTAG1510.10137289785505689No Hit
CTAGATCGAATACCGAGAAATACCCCAAGCCTTATTCGAACTA1500.10070155416065255No Hit
CTAGATCGAGCGGGGCCGACGATCTAGCCGCTGATCGTCGGAC1500.10070155416065255No Hit
CTAGATCGCGTTGATCTAGCAACGGATCGTCGGACTGTAGAAC1500.10070155416065255No Hit
CTAGATCGCGTTGCTATAGTGAGTCGTATTACCGGCCGACGAT1490.1000302104662482No Hit
CTAGATCGCAGTATACCGGCCGACGATCTAGTACTGGATCGTC1490.1000302104662482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC308.251631E-637.00772511
TCGCCTT452.582965E-937.0077256
GTGTTGG355.578404E-737.0077259
CGGGTTG1900.037.0077257
GAAATGG750.037.0077258
CGGCGGA600.037.0077257
TCGCATG2500.037.0077256
GCGACTT355.578404E-737.0077258
GCGACTA355.578404E-737.0077258
GGTGTTG452.582965E-937.0077258
GTCGATG355.578404E-737.0077258
TTGGAGT452.582965E-937.00772511
GTCGAGG1350.037.0077258
CTCAACG355.578404E-737.00772510
GAAGACG355.578404E-737.00772511
CGAAAGT600.037.00772510
CCAGGGG551.2732926E-1137.0077259
CGTTGTG551.2732926E-1137.0077259
CGTTGTA1100.037.0077259
GACGTAG900.037.0077259
Produced by FastQC (version 0.11.5)