FastQCFastQC Report
Thu 26 May 2016
SRR2099156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2099156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences608526
Sequences flagged as poor quality0
Sequence length42
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGCAGCCTGAGGCGATCGTCGGACTGTAGAACTCTGAA36900.6063832934007749Illumina DpnII expression Sequencing Primer (100% over 25bp)
CTGCTGCAGTCAGCCCGACAACGGCTGCCCGTTGGTGGGGGA28590.46982380374873056No Hit
CTGATGCGGCAATCCGGGCTTCACCGCGACGTGCTCGAACAG22690.37286820941093723No Hit
CTGATGCAGAAAAGGTGGCCTCCACCTCCACCAGCCCAGCTT11440.18799525410582293No Hit
CTGATGCATTAAGGGCAATAACCCACCCCCCCATAGAGGCTG9930.1631811952159809No Hit
CTGATTGATATTCGCTTTCGCCCTGCCAGCCGACTTCCTGCA8990.14773403272826469No Hit
CTGATGCACGTCGAATTGTGGGGGCAATCTAGCTGCATTAAG8420.1383671363261389No Hit
CTGATGCCGAAGCCCCCGCAGGGCTGTGGGCAGGAACCCTGG7490.12308430535424945No Hit
CTGATGCAACGTTGGGGATGATGGAGTCTGCTTGAATGAATT7470.12275564232259592No Hit
CTGATGCACCACACGATCCCCTCTTTATCTTCTTAAGTTAGT7230.11881168594275347No Hit
CTGATGTAACAACAAACTGGAGTTCCCATTGTGGTGCAGCAG7080.11634671320535195No Hit
CTGACGCTCCTCGCTCAGCGCAAGCCTAATCGAATTGCATCG6880.11306008288881658No Hit
CTGATGCACCGTGGAAAGGAAGACTAGCCACCTGACTTGGTC6860.11273141985716303No Hit
CTGATGCACACTGAAAGAAGGTGGATGCCAAGAAGCAGAAGC6670.10960912105645446No Hit
CTGATGCAAGGTGCTGTGACGAGGTAGCCACACCACGCCACC6370.1046791755816514No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG950.036.022718
ACGCATG251.4432242E-436.022718
ACGTTAT251.4432242E-436.022718
GACCGCT200.0020998236.022719
TACGGAG404.845606E-836.022719
CGTTCGT200.0020998236.022719
CCGTCAG251.4432242E-436.022719
AAACGGG404.845606E-836.022718
GCGGAAC251.4432242E-436.022719
CGCGTTT900.036.022719
ACGCGCC404.845606E-836.022718
CATAGCG200.002100668136.0197457
CATACCG750.036.0197457
CACGTCG1550.036.0197457
CACCGTT404.849062E-836.0197457
CACGTAG453.4015102E-936.0197457
CAATACC700.036.0197457
GCACGTC3350.036.016786
GCACGTA1100.036.016786
GCACGAG1300.036.016786
Produced by FastQC (version 0.11.5)